##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513209_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2626401 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.317526912303187 31.0 30.0 34.0 24.0 34.0 2 29.14411660671771 31.0 30.0 34.0 22.0 34.0 3 29.357231054968377 31.0 30.0 34.0 23.0 34.0 4 32.44613674758729 35.0 33.0 37.0 22.0 37.0 5 32.578429569589716 37.0 33.0 37.0 23.0 37.0 6 32.93430706125988 37.0 35.0 37.0 26.0 37.0 7 32.87190036860327 37.0 35.0 37.0 26.0 37.0 8 32.77120020895514 37.0 35.0 37.0 25.0 37.0 9 33.98260242818976 39.0 34.0 39.0 23.0 39.0 10 33.57115459520462 38.0 34.0 39.0 18.0 39.0 11 34.02170460641768 39.0 34.0 39.0 25.0 39.0 12 34.0198846253866 39.0 34.0 39.0 25.0 39.0 13 34.06125454566915 39.0 35.0 39.0 25.0 39.0 14 34.281918488456256 39.0 33.0 41.0 17.0 41.0 15 34.704878272586704 39.0 34.0 41.0 19.0 41.0 16 34.54159703716226 39.0 34.0 41.0 17.0 41.0 17 34.15619815862087 39.0 33.0 41.0 17.0 41.0 18 33.88723161466966 39.0 33.0 41.0 15.0 41.0 19 34.51825178257243 39.0 34.0 41.0 18.0 41.0 20 34.72895837307403 39.0 34.0 41.0 19.0 41.0 21 34.77384032369771 40.0 35.0 41.0 19.0 41.0 22 34.458934488678615 40.0 34.0 41.0 17.0 41.0 23 33.99425792177204 39.0 34.0 41.0 10.0 41.0 24 34.23767810018348 39.0 34.0 41.0 10.0 41.0 25 33.86602464741675 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1329374756974282 1101 2 1.4093134070998907 1101 3 1.3224768159637996 1101 4 1.65866358505302 1101 5 1.5849220131352268 1101 6 1.267615951993605 1101 7 1.215972866379083 1101 8 1.2270171135962684 1101 9 1.7337932283730453 1101 10 2.0283460828967677 1101 11 1.6329041821672874 1101 12 1.6436083534022714 1101 13 1.5372900717812215 1101 14 2.5254428382187726 1101 15 2.2173221594918573 1101 16 2.15264635359636 1101 17 2.590834403936917 1101 18 2.6279045480634196 1101 19 2.1903814531933605 1101 20 1.8316009633877286 1101 21 1.6945905411919853 1101 22 2.0641121282569372 1101 23 2.4022976419173006 1101 24 2.021721617351936 1101 25 2.2624156422882535 1102 1 0.982077070969364 1102 2 1.1784837130715857 1102 3 1.0824830501283245 1102 4 1.423039986052089 1102 5 1.3848655355557113 1102 6 1.0715305346871844 1102 7 1.033276336405578 1102 8 1.1267922910686536 1102 9 1.4137055774702532 1102 10 1.6701117468290434 1102 11 1.2961778387335912 1102 12 1.1882137630466474 1102 13 1.161438344370005 1102 14 2.2037664506232844 1102 15 1.7338401948072857 1102 16 1.8376831959332662 1102 17 2.2292387954094437 1102 18 2.4418652505709915 1102 19 1.8367504712211726 1102 20 1.6159109991672835 1102 21 1.4748117352507961 1102 22 1.721196194702678 1102 23 2.05866814284105 1102 24 1.8759977090684217 1102 25 2.1941963652412824 1103 1 1.0749018356872746 1103 2 1.3316052825981615 1103 3 1.1105951905055278 1103 4 1.4967137761929692 1103 5 1.3776230803640246 1103 6 1.0418844205885165 1103 7 1.0678550122353911 1103 8 1.085628719148069 1103 9 1.468957787365106 1103 10 1.785825647578804 1103 11 1.2886085890755226 1103 12 1.2777539580215986 1103 13 1.2320475368973547 1103 14 2.2224470843415247 1103 15 1.795098648784105 1103 16 1.7384698103128216 1103 17 2.232413068918298 1103 18 2.4243431615479096 1103 19 1.8224748631402932 1103 20 1.4583804338351882 1103 21 1.4985903130174592 1103 22 1.787552344592406 1103 23 2.1132524960113273 1103 24 1.8204937656988989 1103 25 2.0116114334189916 1104 1 0.9328974955303622 1104 2 1.1959234415897448 1104 3 1.078864331312115 1104 4 1.452054656792484 1104 5 1.4143124349958995 1104 6 1.248462407982565 1104 7 1.077195779382805 1104 8 1.1903315053303842 1104 9 1.4917169671014747 1104 10 1.7174287626494475 1104 11 1.3218166585160702 1104 12 1.2701010978866805 1104 13 1.2681580893427977 1104 14 2.2166631143380613 1104 15 1.7311984699374179 1104 16 1.8766415812191326 1104 17 2.2320549089739146 1104 18 2.384203094856737 1104 19 1.7882901724458407 1104 20 1.5165717862800463 1104 21 1.5491361723471613 1104 22 1.805862212739946 1104 23 2.056663838716986 1104 24 1.8581685820084033 1104 25 2.0184482733309537 1105 1 0.8458611952116577 1105 2 1.1257314354687118 1105 3 1.0275511792103948 1105 4 1.3397132032731136 1105 5 1.242900677704398 1105 6 0.9476551570767455 1105 7 0.9403175024108847 1105 8 1.047525691134723 1105 9 1.302490348476141 1105 10 1.6703249300601541 1105 11 1.0935560083727225 1105 12 1.0906828079459672 1105 13 0.9934942366848603 1105 14 1.8870592534379966 1105 15 1.5218892970994133 1105 16 1.8185884958126692 1105 17 2.035295039961703 1105 18 1.6988577600535422 1105 19 1.4561776078295807 1105 20 1.3953899863155925 1105 21 1.3725847789401726 1105 22 1.4627350343490875 1105 23 1.8093198815405955 1105 24 1.6827517120375646 1105 25 1.845700986118409 1106 1 0.6409035671394356 1106 2 0.7844045449253656 1106 3 0.8122498302165901 1106 4 1.3065600195322702 1106 5 1.112441763455081 1106 6 0.8548270742878401 1106 7 0.8099268352915203 1106 8 0.7937461699702908 1106 9 1.0714919149801574 1106 10 0.8642424229492391 1106 11 0.86547776927587 1106 12 0.7491619390010058 1106 13 0.7617174434487097 1106 14 1.8517933440290903 1106 15 1.3449301152430735 1106 16 1.4978805553691785 1106 17 1.7436334694068876 1106 18 1.891998605496049 1106 19 1.356652010997422 1106 20 1.1783844159799557 1106 21 1.1277977344469647 1106 22 1.2767735912867622 1106 23 1.7581161880744176 1106 24 1.315751914577433 1106 25 1.6480311241488437 1107 1 0.6732574446430455 1107 2 0.8986327654710671 1107 3 0.8427252681765793 1107 4 1.1034230171293373 1107 5 1.0654218607673087 1107 6 0.7779677190876981 1107 7 0.7546645755427335 1107 8 0.7677033904485882 1107 9 1.0788892789746285 1107 10 1.229775118719708 1107 11 0.9269005554800813 1107 12 0.905151769925908 1107 13 0.8311705915647281 1107 14 1.6236428260391378 1107 15 1.0548385342946247 1107 16 1.2600706349522 1107 17 1.6549579539145114 1107 18 1.8561356944178584 1107 19 1.3854068705141884 1107 20 1.2002046201931833 1107 21 1.0869797056337873 1107 22 1.3321046837333768 1107 23 1.7116292607899481 1107 24 1.475193896500251 1107 25 1.8344434539970464 1108 1 0.7021259995896685 1108 2 0.9633969146436243 1108 3 0.7581759004011559 1108 4 1.180449510990556 1108 5 1.0348901347881991 1108 6 0.6987609695033115 1108 7 0.6788666890732813 1108 8 0.7244226935034277 1108 9 0.9455972415830303 1108 10 1.1546157964264907 1108 11 0.9407339876261673 1108 12 0.5874768943626947 1108 13 0.7562393532035614 1108 14 1.6432778775811485 1108 15 1.2685294493999422 1108 16 1.3883143674253304 1108 17 1.6054481572846342 1108 18 1.8847017902371945 1108 19 1.3030869877578297 1108 20 0.9998814068447501 1108 21 1.058414672317241 1108 22 1.2553370863851043 1108 23 1.5988244021771933 1108 24 1.3607042626316286 1108 25 1.6317529844345628 1109 1 0.5597977177372115 1109 2 0.7419667343568257 1109 3 0.6638333555988787 1109 4 0.8805936202709503 1109 5 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2215 18 -3.5821773128809724 2215 19 -4.148975618832022 2215 20 -4.003065608369049 2215 21 -4.175469820092076 2215 22 -10.157179501766564 2215 23 -11.339195709014103 2215 24 -7.5385749129203425 2215 25 -5.911616417148714 2216 1 -2.5564785326142534 2216 2 -2.584049827280822 2216 3 -2.4227689714679954 2216 4 -7.843788044711246 2216 5 -4.370212667496972 2216 6 -3.6065587968301465 2216 7 -2.8574259516785254 2216 8 -8.887652743531937 2216 9 -4.863098924073213 2216 10 -3.1667146935710804 2216 11 -2.998520287900096 2216 12 -3.3331847778970527 2216 13 -2.9859602903643463 2216 14 -2.4113462816614977 2216 15 -3.0593692601059175 2216 16 -2.7493425496004633 2216 17 -2.3945987612217614 2216 18 -8.499285703443046 2216 19 -5.957088757330332 2216 20 -5.302639466319796 2216 21 -4.333236012346326 2216 22 -3.9026283637676364 2216 23 -3.1438686903787847 2216 24 -3.511108930901891 2216 25 -2.893669418298053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 124646.0 3 2.0 4 1063.0 5 6103.0 6 7524.0 7 6712.0 8 6070.0 9 5831.0 10 5415.0 11 5355.0 12 5152.0 13 5071.0 14 5092.0 15 5197.0 16 5362.0 17 5588.0 18 6429.0 19 7747.0 20 10113.0 21 13066.0 22 16134.0 23 19223.0 24 22073.0 25 25251.0 26 28778.0 27 32328.0 28 36182.0 29 43022.0 30 51640.0 31 61252.0 32 78237.0 33 101261.0 34 132851.0 35 177530.0 36 250548.0 37 360779.0 38 951774.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56481297846178 18.414580936892627 12.457588720430337 25.563017364215252 2 14.49586086648508 19.73760550133747 41.52538749984732 24.24114613233013 3 18.752366600096497 24.07286532671296 29.409777570127705 27.764990503062837 4 11.507131424533691 16.342933921048466 38.79944603464348 33.35048861977436 5 12.888524500059544 37.88287545535881 36.09285350042292 13.135746544158735 6 32.49647075937986 36.89828208714739 17.86905841345449 12.736188740018257 7 28.652520621187076 31.196752401044957 21.91268650464215 18.238040473125814 8 25.29079808055178 36.73638965971722 20.386273530891334 17.586538728839667 9 26.446955133575635 15.110810349712558 19.995788155653926 38.44644636105788 10 13.970381022162442 28.765055882133407 35.40819015615313 21.856372939551026 11 34.673656566702874 22.41854552600191 24.250385774847633 18.657412132447586 12 23.168467965470516 24.575446224204004 31.522894102825227 20.733191707500254 13 28.8107578422923 21.580618882893944 25.683247826986083 23.925375447827673 14 20.589492060662977 21.43739055658001 28.473645605544394 29.499471777212616 15 23.30070165535204 30.20793695957473 23.214161241702577 23.27720014337065 16 22.413439197889005 27.994863418652326 27.42008270779486 22.171614675663808 17 21.83619133230237 28.163427770456085 27.999032521006484 22.001348376235057 18 21.745098390206948 27.380878475421206 29.31390983528824 21.560113299083604 19 23.463895020512375 27.082314569803774 27.367911119642063 22.085879290041788 20 23.674985813144836 27.11822125400374 27.559213457898363 21.647579474953062 21 23.28877107185569 26.72745416181052 26.961196553851778 23.022578212482003 22 23.260476148575787 27.53641240358622 27.047015681797664 22.15609576604033 23 22.41371167936309 27.260886157230484 27.59059197752653 22.73481018587989 24 23.054981732688145 26.95955059860634 27.65641683373451 22.329050834971 25 22.917406215101565 26.907006577093068 27.53089344560758 22.644693762197786 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 173.0 1 173.0 2 320.0 3 467.0 4 467.0 5 467.0 6 905.5 7 1344.0 8 1344.0 9 1344.0 10 2280.0 11 3216.0 12 3216.0 13 3216.0 14 6282.5 15 9349.0 16 9349.0 17 9349.0 18 17901.5 19 26454.0 20 26454.0 21 26454.0 22 43815.5 23 61177.0 24 61177.0 25 61177.0 26 87852.5 27 114528.0 28 114528.0 29 114528.0 30 148567.5 31 182607.0 32 182607.0 33 182607.0 34 213483.0 35 244359.0 36 244359.0 37 244359.0 38 270107.5 39 295856.0 40 295856.0 41 295856.0 42 313697.5 43 331539.0 44 331539.0 45 331539.0 46 342329.0 47 353119.0 48 353119.0 49 353119.0 50 340627.0 51 328135.0 52 328135.0 53 328135.0 54 293758.0 55 259381.0 56 259381.0 57 259381.0 58 222691.0 59 186001.0 60 186001.0 61 186001.0 62 150919.5 63 115838.0 64 115838.0 65 115838.0 66 89671.5 67 63505.0 68 63505.0 69 63505.0 70 46681.0 71 29857.0 72 29857.0 73 29857.0 74 21097.5 75 12338.0 76 12338.0 77 12338.0 78 8539.0 79 4740.0 80 4740.0 81 4740.0 82 3187.5 83 1635.0 84 1635.0 85 1635.0 86 1102.5 87 570.0 88 570.0 89 570.0 90 373.5 91 177.0 92 177.0 93 177.0 94 99.0 95 21.0 96 21.0 97 21.0 98 18.0 99 15.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16969990492693232 2 0.24859113288488696 3 0.2516371262423369 4 0.4216797054219824 5 0.24706813620616197 6 0.018352109978636164 7 0.017552536722305542 8 0.016676813632038674 9 0.01800943572592304 10 0.019684732072520535 11 0.019684732072520535 12 0.02676666662859175 13 0.025510194368643633 14 0.023416073935396766 15 0.039521763812913564 16 0.020179705993106156 17 0.03887449022445544 18 0.029850734903009858 19 0.025472119451675504 20 0.027832764303699246 21 0.03278250350955547 22 0.0429485063400448 23 0.027680464635826744 24 0.046451398701112284 25 0.03521929819551546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2626401.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.157945354471 #Duplication Level Percentage of deduplicated Percentage of total 1 83.08552611489478 60.78366379260945 2 11.522136516730097 16.858716673153904 3 2.94588597515457 6.465448955725817 4 1.023170800021702 2.9941229390511217 5 0.4829962947018901 1.7667508267106427 6 0.25355869451296015 1.1129899870398088 7 0.1547876209560348 0.7926761020815114 8 0.10153148090960933 0.5942267625714972 9 0.07393604939197057 0.4868108515028927 >10 0.3269836571060977 4.126955669477387 >50 0.01730806599865881 0.8733493533559502 >100 0.009918054585612021 1.3966929322013453 >500 0.0015772151414615286 0.7691234083742466 >1k 6.834598945711477E-4 0.97847174614429 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3228 0.12290583197310692 No Hit GTACATGGGAAGCAGTGGTATCAAC 2728 0.1038683734890445 No Hit CTGTAGGACGTGGAATATGGCAAGA 2675 0.10185040288973389 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.807491696812482E-5 0.0 0.0 0.0 3.807491696812482E-5 7 3.807491696812482E-5 0.0 0.0 0.0 7.614983393624964E-5 8 3.807491696812482E-5 0.0 0.0 0.0 7.614983393624964E-5 9 3.807491696812482E-5 0.0 0.0 1.1422475090437447E-4 7.614983393624964E-5 10 3.807491696812482E-5 0.0 0.0 1.1422475090437447E-4 7.614983393624964E-5 11 3.807491696812482E-5 0.0 0.0 1.1422475090437447E-4 7.614983393624964E-5 12 3.807491696812482E-5 0.0 0.0 1.1422475090437447E-4 7.614983393624964E-5 13 3.807491696812482E-5 0.0 0.0 1.1422475090437447E-4 7.614983393624964E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 640 0.0 12.803269 4 ACCGCTC 60 0.00593174 11.069659 8 GCCGGTT 95 1.37836705E-5 10.987474 11 GTCCTAC 1640 0.0 10.8913355 1 GTAGGAC 1580 0.0 10.492877 3 GTATTAA 310 0.0 10.476124 1 GGACTGT 455 0.0 10.217368 6 AGGACGT 1585 0.0 10.200985 5 CCGCCGG 75 0.0026712269 10.1229515 19 CGTGCGC 150 2.4905603E-8 10.121795 10 GGACGTG 1585 0.0 10.11607 6 TTATACT 415 0.0 10.101991 4 GTATTAC 315 0.0 10.006606 1 TCCTACA 1950 0.0 9.827574 2 CCTACAC 370 0.0 9.785529 3 CCCCTAC 215 9.094947E-12 9.773894 1 GTCCTAA 920 0.0 9.759407 1 TATACTG 410 0.0 9.742892 5 TTAGGAC 1170 0.0 9.6908865 3 TAGGACT 495 0.0 9.624257 4 >>END_MODULE