FastQCFastQC Report
Thu 26 May 2016
SRR1513209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626401
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31500.11993598844959319No Hit
GTCCTACAGTGGACATTTCTAAATT31390.11951716436294382No Hit
GTACATGGGAAGCAGTGGTATCAAC30150.11479587465889633No Hit
CCCATGTACTCTGCGTTGATACCAC30090.11456742515708758No Hit
CTGTAGGACGTGGAATATGGCAAGA29530.11243522980687261No Hit
CTTTAGGACGTGAAATATGGCGAGG27190.1035256992363314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6200.013.6678424
TTGCGCG655.506225E-513.1408218
GGACCGT751.4476267E-512.6935266
GTAGGAC17100.010.6909193
TTTGCGC909.612269E-510.54550517
ATAGGAC2750.010.3871993
AGGACCT12700.010.34610755
GTCCTAT3050.010.30581
GTCCTAA8700.010.1819581
TAGGACA5650.010.1112394
GTCCTAC16800.010.0353281
GACCGTG1356.5367203E-79.84190757
GGCGAGG7750.09.79412919
TAGACAG3700.09.7787535
TGGCGAG16550.09.74864518
CTACACT4700.09.7240014
TGTAGGA19300.09.7194962
AGGACGT16550.09.6077145
GGACGTG16700.09.5771516
CCTACAC4800.09.52163
Produced by FastQC (version 0.11.5)