##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513209_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2626401
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.73617280834115	31.0	31.0	34.0	27.0	34.0
2	30.82838644974625	31.0	31.0	34.0	27.0	34.0
3	30.93334376586058	33.0	31.0	34.0	27.0	34.0
4	34.32927645093038	37.0	35.0	37.0	31.0	37.0
5	34.07666498756283	37.0	35.0	37.0	30.0	37.0
6	34.004889961586215	37.0	35.0	37.0	30.0	37.0
7	33.86393319222769	37.0	35.0	37.0	29.0	37.0
8	33.82812868255837	37.0	35.0	37.0	29.0	37.0
9	35.37862915830446	39.0	35.0	39.0	29.0	39.0
10	35.2181749854649	39.0	35.0	39.0	28.0	39.0
11	35.23712068339907	39.0	35.0	39.0	29.0	39.0
12	35.17157585608595	39.0	35.0	39.0	28.0	39.0
13	35.149268142983495	39.0	35.0	39.0	28.0	39.0
14	36.29895282555862	40.0	36.0	41.0	28.0	41.0
15	36.28302723003837	40.0	36.0	41.0	28.0	41.0
16	36.24304590197765	40.0	36.0	41.0	27.0	41.0
17	36.168023847082	40.0	36.0	41.0	27.0	41.0
18	36.122077321779884	40.0	36.0	41.0	27.0	41.0
19	36.20069859857653	40.0	37.0	41.0	27.0	41.0
20	36.16602605618868	40.0	37.0	41.0	27.0	41.0
21	36.11020365892337	40.0	37.0	41.0	27.0	41.0
22	36.04958191837423	40.0	37.0	41.0	27.0	41.0
23	35.97100785447462	40.0	36.0	41.0	27.0	41.0
24	35.91073678391076	40.0	36.0	41.0	27.0	41.0
25	35.85696357867668	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.607093452744671
1101	2	0.6481182073243019
1101	3	0.6057481564109324
1101	4	0.7488225113273757
1101	5	0.6580019176179519
1101	6	0.6739418225127878
1101	7	0.7968984242982202
1101	8	0.7702299749093129
1101	9	0.8025727311340418
1101	10	0.968520894638786
1101	11	0.8804055668453898
1101	12	0.814404978477441
1101	13	0.9165764670571122
1101	14	0.9629482093681361
1101	15	1.0762694064013871
1101	16	0.9996517808693639
1101	17	1.0569057490031568
1101	18	0.9919717157346923
1101	19	0.9467456841899278
1101	20	0.9219836890619888
1101	21	0.9499174763790421
1101	22	0.9738080971304655
1101	23	0.9683101868268977
1101	24	1.0508538882459817
1101	25	1.0360566360844388
1102	1	0.4557375407161608
1102	2	0.469220816721009
1102	3	0.4390445215404206
1102	4	0.56548418499424
1102	5	0.5445133036580216
1102	6	0.5601249959200842
1102	7	0.4857472844438746
1102	8	0.5634271960673587
1102	9	0.5172826211746866
1102	10	0.6824528269647132
1102	11	0.7303836359898526
1102	12	0.6307066810320237
1102	13	0.7017549222046355
1102	14	0.7469969253753064
1102	15	0.7526887603688976
1102	16	0.8204773788040143
1102	17	0.8215224641661081
1102	18	0.7565269769894485
1102	19	0.7306896222213837
1102	20	0.7525215051588887
1102	21	0.8578342651616495
1102	22	0.802073118891272
1102	23	0.8154374323014437
1102	24	0.7927487866838661
1102	25	0.8568911739060354
1103	1	0.5475050000268986
1103	2	0.5727531249569253
1103	3	0.5426135538317851
1103	4	0.6491343873735644
1103	5	0.6895590548833184
1103	6	0.4343429968610124
1103	7	0.5434667057124685
1103	8	0.6699965588752264
1103	9	0.7160311001040114
1103	10	0.6970971644131296
1103	11	0.6601074413166259
1103	12	0.7738446423903795
1103	13	0.7103933877743458
1103	14	0.7582248090573174
1103	15	0.786084326088087
1103	16	0.8214988286300198
1103	17	0.9338721436742787
1103	18	0.8531248902690649
1103	19	0.8038962972651049
1103	20	0.8503830485220547
1103	21	0.7130722568917847
1103	22	0.8361284769309663
1103	23	0.770985320469741
1103	24	0.8997233547791978
1103	25	0.766814261823427
1104	1	0.37874241623625693
1104	2	0.4378870586041437
1104	3	0.38969804348026216
1104	4	0.6085570880591646
1104	5	0.5301006487085758
1104	6	0.4967114495142866
1104	7	0.3966884139622735
1104	8	0.5039994315217982
1104	9	0.4201154740902808
1104	10	0.5598727922489672
1104	11	0.6076289671344668
1104	12	0.600970033732608
1104	13	0.6019099698307926
1104	14	0.7008058886351307
1104	15	0.667413793209441
1104	16	0.6523891354246487
1104	17	0.6449813393602497
1104	18	0.6255204461880624
1104	19	0.7006542683190986
1104	20	0.692605059556378
1104	21	0.5832085197450851
1104	22	0.6055077293896431
1104	23	0.5771541190291458
1104	24	0.6482652373916338
1104	25	0.5520472180390144
1105	1	0.37634880196486975
1105	2	0.34771035507110426
1105	3	0.32025245703122707
1105	4	0.40330423347166544
1105	5	0.3510272578313405
1105	6	0.3290226818994455
1105	7	0.35640526192456434
1105	8	0.4097350217078599
1105	9	0.4425219734558681
1105	10	0.45210672883973047
1105	11	0.3923387127855662
1105	12	0.3940270260256753
1105	13	0.4757431351868746
1105	14	0.5163306792708156
1105	15	0.5794367136108747
1105	16	0.5841138916935122
1105	17	0.6131882187302651
1105	18	0.5860634763466663
1105	19	0.6153323699119397
1105	20	0.5549434358178686
1105	21	0.5797502973366448
1105	22	0.48868381894818924
1105	23	0.529077626768327
1105	24	0.5243610489236232
1105	25	0.5101348425879522
1106	1	0.3029325022997966
1106	2	0.2797802440459911
1106	3	0.33089387242288737
1106	4	0.44960636424979583
1106	5	0.3874947081087825
1106	6	0.42869113293910033
1106	7	0.41172286303060446
1106	8	0.4637307321678179
1106	9	0.3793302680016808
1106	10	0.43034690198685865
1106	11	0.46275727561353364
1106	12	0.4742677940725173
1106	13	0.39912435729483065
1106	14	0.5353571963384667
1106	15	0.5644680877215862
1106	16	0.6215539247261006
1106	17	0.49658738567497807
1106	18	0.5877471501654981
1106	19	0.6694863452216424
1106	20	0.6703099498131806
1106	21	0.5232455875508393
1106	22	0.5545855959175512
1106	23	0.47793655432461435
1106	24	0.6055643705774827
1106	25	0.6459060253718647
1107	1	0.23305870991928757
1107	2	0.3431285532711499
1107	3	0.282682901160058
1107	4	0.3059311331703043
1107	5	0.31860811523650057
1107	6	0.27399681464810044
1107	7	0.3073392717897576
1107	8	0.3031669923726241
1107	9	0.392628601820185
1107	10	0.40205331714649617
1107	11	0.4347844320248413
1107	12	0.41919207571488926
1107	13	0.443661502812418
1107	14	0.463976493680903
1107	15	0.4400870442465674
1107	16	0.49883167756796354
1107	17	0.4382047102914157
1107	18	0.463577982338478
1107	19	0.4552100373884329
1107	20	0.4817880650783124
1107	21	0.4454532863924854
1107	22	0.4310076005838326
1107	23	0.4271167521134558
1107	24	0.46863151735221464
1107	25	0.4538452317595443
1108	1	0.34085599288715684
1108	2	0.38645777117701385
1108	3	0.3626440277246843
1108	4	0.4654496352397075
1108	5	0.3830219926562606
1108	6	0.312531311634487
1108	7	0.2674454795685577
1108	8	0.3666695965669575
1108	9	0.4220220331446072
1108	10	0.498814737811891
1108	11	0.42734301665779384
1108	12	0.39055408387348933
1108	13	0.46740241929898474
1108	14	0.41437603014635016
1108	15	0.5395929096161254
1108	16	0.5265959379491463
1108	17	0.5018985649877834
1108	18	0.5531168632113719
1108	19	0.6578197567389452
1108	20	0.6847523061927774
1108	21	0.5275022309376354
1108	22	0.44184229569157196
1108	23	0.5160701939495738
1108	24	0.5580223561698361
1108	25	0.5396572070159777
1109	1	0.2632256361719776
1109	2	0.29850587508624216
1109	3	0.27676984638263136
1109	4	0.37703948589742
1109	5	0.3322303383941687
1109	6	0.3945463705808976
1109	7	0.2926634347737007
1109	8	0.29396126900489605
1109	9	0.1920233177264805
1109	10	0.2882475148476331
1109	11	0.26371335167651466
1109	12	0.41744737228970763
1109	13	0.39757510848055233
1109	14	0.3603753758161119
1109	15	0.3849519280412892
1109	16	0.21183854684025505
1109	17	0.3848821565875298
1109	18	0.4058970011209979
1109	19	0.3497103982619407
1109	20	0.35708819852074214
1109	21	0.34215272940566166
1109	22	0.35403259194373504
1109	23	0.3499850957188002
1109	24	0.3769843859634818
1109	25	0.34323487857516
1110	1	0.19669027514477122
1110	2	0.20607906060138248
1110	3	0.1269428554246801
1110	4	0.04634023676967303
1110	5	0.13395454403373463
1110	6	0.16107454561069545
1110	7	0.17319388329833885
1110	8	0.14499454626869124
1110	9	0.17639254247927738
1110	10	0.19131608813642487
1110	11	0.07067917387711731
1110	12	0.13180901599107386
1110	13	0.19138023947026284
1110	14	0.348490755972378
1110	15	0.2168713313948416
1110	16	0.2374338856248599
1110	17	0.2964143812140705
1110	18	0.17709144401218424
1110	19	0.15908980122395633
1110	20	0.07535982195478397
1110	21	0.06039592536218663
1110	22	0.0929501251795628
1110	23	0.2086521764302134
1110	24	0.10497984788302972
1110	25	0.19802228265879762
1111	1	0.022042644130440436
1111	2	0.06049684359466667
1111	3	0.059563653326470956
1111	4	0.17203646788779992
1111	5	0.07543829869960206
1111	6	0.039154864844583415
1111	7	0.11241930587964077
1111	8	0.01511043411565538
1111	9	-0.030170269052710808
1111	10	0.03776345973570727
1111	11	0.10191755829964677
1111	12	0.04445670750900632
1111	13	-0.049130251455480334
1111	14	-0.0065536379585964255
1111	15	0.07384926094754007
1111	16	0.12253004296611891
1111	17	0.036054018952278
1111	18	0.13076925389439253
1111	19	-0.008060307975661374
1111	20	0.021059364355259902
1111	21	0.05323222864844723
1111	22	0.004202012692580581
1111	23	0.004967976148286368
1111	24	0.08584910171181548
1111	25	0.08446300355216607
1112	1	-0.5713576012209849
1112	2	-0.46518052117593456
1112	3	-0.2933770641325424
1112	4	-1.1345620098740312
1112	5	-0.5444522626481785
1112	6	-0.42911130739892656
1112	7	-0.4146165245081832
1112	8	-0.22759511390994902
1112	9	-0.19258777980456898
1112	10	-0.09596335176459547
1112	11	-0.08561655112860223
1112	12	-0.15500955383281934
1112	13	-0.1635319732663163
1112	14	-0.15160241503214422
1112	15	-0.1765658454738741
1112	16	-0.21147066074035337
1112	17	-0.13615319328237518
1112	18	-0.08343996541704968
1112	19	-0.10019525314993416
1112	20	-0.1157232262871446
1112	21	-0.10848441315834378
1112	22	-0.17692283467216185
1112	23	-0.0944187398787264
1112	24	-0.04244030041197533
1112	25	-0.058110268465377146
1113	1	-0.43329793531344407
1113	2	-0.3442446197786069
1113	3	-0.36649841393017013
1113	4	-1.2544388519030036
1113	5	-0.5813152534585413
1113	6	-0.37771283404659783
1113	7	-0.22652691311287754
1113	8	-0.2006699242234049
1113	9	-0.12237657828478632
1113	10	-0.15049656455303762
1113	11	-0.17193108174738825
1113	12	-0.26487182366264506
1113	13	-0.2616201350711336
1113	14	-0.23181006886993316
1113	15	-0.1557399031446849
1113	16	-0.21982583329884164
1113	17	-0.28855331459021727
1113	18	-0.239266651615047
1113	19	-0.20509744393892504
1113	20	-0.1889780909983898
1113	21	-0.14745646276485047
1113	22	-0.2195450866491342
1113	23	-0.165080554231956
1113	24	-0.13449787967215343
1113	25	-0.14383399559141452
1114	1	-0.44445368656222684
1114	2	-0.39971165651106233
1114	3	-0.28557555284951874
1114	4	-1.1642219903045614
1114	5	-0.5408250305053244
1114	6	-0.4792896112761298
1114	7	-0.3769065922368995
1114	8	-0.4357859124361241
1114	9	-0.3555337306056927
1114	10	-0.24803907262442948
1114	11	-0.2594733423468014
1114	12	-0.3100235620581131
1114	13	-0.26674899182673784
1114	14	-0.3706175775504903
1114	15	-0.2939401536624757
1114	16	-0.28523648449279904
1114	17	-0.3713155587422605
1114	18	-0.27783849082678813
1114	19	-0.44876929990262227
1114	20	-0.4336721965965893
1114	21	-0.47078193410379043
1114	22	-0.41055585739109546
1114	23	-0.5009124027334195
1114	24	-0.46477969314680934
1114	25	-0.4041564325431679
1115	1	-0.4160239953701179
1115	2	-0.3376076350673003
1115	3	-0.2916781454475803
1115	4	-0.13970639484142566
1115	5	-0.294587288303056
1115	6	-0.2801382422425789
1115	7	-0.3965924492489492
1115	8	-0.40493235250583126
1115	9	-0.378476026653658
1115	10	-0.4258874414512235
1115	11	-0.383847530083095
1115	12	-0.48059386348178634
1115	13	-0.43030521155145607
1115	14	-0.45017280779033086
1115	15	-0.48038744144012213
1115	16	-0.4779183577205899
1115	17	-0.5005319457932984
1115	18	-0.6225738220826926
1115	19	-0.6492659806977557
1115	20	-0.66646558993898
1115	21	-0.5573198631267076
1115	22	-0.5945257408681783
1115	23	-0.43136693811857896
1115	24	-0.5644721405711977
1115	25	-0.6529334218508254
1116	1	-0.3789770418409333
1116	2	-0.31873537464661794
1116	3	-0.32254122521292317
1116	4	-0.17552618743085446
1116	5	-0.3980795088579825
1116	6	-0.4483900364636142
1116	7	-0.46640773299581895
1116	8	-0.48803465253018175
1116	9	-0.4412448143315899
1116	10	-0.47870974483028306
1116	11	-0.5431661025402761
1116	12	-0.46757500944830355
1116	13	-0.5172940771596402
1116	14	-0.4857523146195888
1116	15	-0.5189976224085342
1116	16	-0.5285388953983983
1116	17	-0.5618139281019765
1116	18	-0.6907307528675304
1116	19	-0.631457077091774
1116	20	-0.7066421552988515
1116	21	-0.7377871563868439
1116	22	-0.5648020825966995
1116	23	-0.782131015862042
1116	24	-0.8167294209795557
1116	25	-0.7059860040471406
1201	1	0.4248425425567035
1201	2	0.46226609905464144
1201	3	0.3727517288765476
1201	4	0.42636972383960625
1201	5	0.45193384443275164
1201	6	0.37071055805598263
1201	7	0.4012289668300113
1201	8	0.398433652777058
1201	9	0.44057909344759594
1201	10	0.601027290566833
1201	11	0.5225388940756659
1201	12	0.5330263101203272
1201	13	0.46182789511359346
1201	14	0.5863029413411667
1201	15	0.6936045621111475
1201	16	0.5687927006230069
1201	17	0.657613139858185
1201	18	0.6375494563067576
1201	19	0.6124231537874607
1201	20	0.6274111588305544
1201	21	0.7148529331204756
1201	22	0.7149214777471542
1201	23	0.7855942708871524
1201	24	0.5453727347854453
1201	25	0.6126433694834006
1202	1	0.4376445253056538
1202	2	0.4581648918687975
1202	3	0.39340201111665607
1202	4	0.4819543528496837
1202	5	0.517041919079972
1202	6	0.4220271644186582
1202	7	0.409271373282877
1202	8	0.3644055677995439
1202	9	0.4745917234632273
1202	10	0.44786573586389977
1202	11	0.47562241348694556
1202	12	0.587901176138999
1202	13	0.5608140434813507
1202	14	0.5420475966688372
1202	15	0.5849312191053784
1202	16	0.612962859017351
1202	17	0.6888971718222336
1202	18	0.5750324796137605
1202	19	0.5303198953847357
1202	20	0.5512621084796976
1202	21	0.5847119734421682
1202	22	0.6120435614587123
1202	23	0.5930937666076943
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2210	17	-0.1640493463362489
2210	18	-0.1486146744499095
2210	19	-0.21954328075318585
2210	20	-0.20869752848098955
2210	21	-0.29348994925886274
2210	22	-0.283167684811211
2210	23	-0.22857261772401927
2210	24	-0.21535975658665762
2210	25	-0.17406582516534996
2211	1	-0.6089244917196375
2211	2	-0.4903830157325473
2211	3	-0.34168840584421645
2211	4	-1.4716821508605165
2211	5	-0.7888850105856022
2211	6	-0.5336247952340116
2211	7	-0.519244788959135
2211	8	-0.37604072902116314
2211	9	-0.5027142544906411
2211	10	-0.3458040830897744
2211	11	-0.4287444096994264
2211	12	-0.40622801488356686
2211	13	-0.37935503766831147
2211	14	-0.5389831657626587
2211	15	-0.42294252708612134
2211	16	-0.4541111231670172
2211	17	-0.5342819123847207
2211	18	-0.41598821028446054
2211	19	-0.5037580412345477
2211	20	-0.5078605825936222
2211	21	-0.5457449239529666
2211	22	-0.4553697909932737
2211	23	-0.477920826491804
2211	24	-0.5028609502609953
2211	25	-0.4496880448050291
2212	1	-0.19305341518359143
2212	2	-0.39679722416101626
2212	3	-0.41434433182265806
2212	4	-0.08603364067422348
2212	5	-0.21065188105981747
2212	6	-0.25130558749393117
2212	7	-0.3338781608296415
2212	8	-0.3369052328622786
2212	9	-0.33609465198880173
2212	10	-0.33012522951910483
2212	11	-0.35574917595340594
2212	12	-0.31818104970493266
2212	13	-0.3834394184864962
2212	14	-0.5194190467961093
2212	15	-0.33922809403556897
2212	16	-0.35382713222414
2212	17	-0.32598237695118115
2212	18	-0.36781027928001464
2212	19	-0.4288300619800438
2212	20	-0.44441071587350933
2212	21	-0.31087285246847784
2212	22	-0.33417570883621295
2212	23	-0.3428922230813214
2212	24	-0.3020612766639843
2212	25	-0.4940908982821952
2213	1	-0.32042335243440334
2213	2	-0.3285963890322492
2213	3	-0.4115268277141624
2213	4	-0.10484110518215317
2213	5	-0.45049207970495075
2213	6	-0.39865148009675266
2213	7	-0.4166886404066261
2213	8	-0.4681766146783559
2213	9	-0.5961970758564021
2213	10	-0.49498478604839136
2213	11	-0.5828736925115834
2213	12	-0.6323512567085388
2213	13	-0.6831972652789489
2213	14	-0.7918383174575041
2213	15	-0.6768726549866315
2213	16	-0.642472610316716
2213	17	-0.7761016345426341
2213	18	-0.8920857284974559
2213	19	-0.7713783769683005
2213	20	-0.7080913166236584
2213	21	-0.8355119357222023
2213	22	-0.9071999582915211
2213	23	-0.8517070781689213
2213	24	-0.8713110755620619
2213	25	-0.8010720766698185
2214	1	-0.5026862547387232
2214	2	-0.49825851688091305
2214	3	-0.6117373696215225
2214	4	-0.34715738356298687
2214	5	-0.5543506040071975
2214	6	-0.6723691846609583
2214	7	-0.7342379431849366
2214	8	-0.7735311503223627
2214	9	-0.8617853764975436
2214	10	-0.964350409527519
2214	11	-0.9766981492288238
2214	12	-1.0416106041344264
2214	13	-1.0939788095350735
2214	14	-1.216438658133356
2214	15	-1.0903296155655653
2214	16	-1.0645443321051573
2214	17	-1.1387693274688502
2214	18	-1.2180130650894299
2214	19	-1.2056408983927938
2214	20	-1.0933301071185966
2214	21	-1.0647870473411487
2214	22	-1.203865672758603
2214	23	-1.1313932226070165
2214	24	-1.163870429335951
2214	25	-1.0710590103755635
2215	1	-0.825138025918605
2215	2	-0.7826450902284741
2215	3	-0.8064520767962122
2215	4	-0.6023621206583769
2215	5	-0.7836781205891228
2215	6	-0.8983358534199866
2215	7	-0.9737513133437403
2215	8	-1.0796638475792832
2215	9	-1.2885123553438191
2215	10	-1.2888069198951442
2215	11	-1.2138876958151457
2215	12	-1.3045374448851632
2215	13	-1.4515207089764104
2215	14	-1.5529615275912292
2215	15	-1.6041483233261147
2215	16	-1.5328778223652364
2215	17	-1.4831856122804368
2215	18	-1.5340138392613767
2215	19	-1.5754610080322777
2215	20	-1.5352515182039
2215	21	-1.5359138336906142
2215	22	-1.6901868913471034
2215	23	-1.5534385535132174
2215	24	-1.5383564942408512
2215	25	-1.6306270956600173
2216	1	-0.6642349745452094
2216	2	-0.5957103490967839
2216	3	-0.6647914141436573
2216	4	-0.4297350404580911
2216	5	-0.8289419541235432
2216	6	-0.8554335987697357
2216	7	-1.0045211709056545
2216	8	-1.0495337034257588
2216	9	-1.084834753747323
2216	10	-1.0958869867588987
2216	11	-1.028773695788459
2216	12	-1.1612554601677587
2216	13	-1.2065257218516834
2216	14	-1.3897197697683765
2216	15	-1.411157390340442
2216	16	-1.4782929951760124
2216	17	-1.6169455165211772
2216	18	-1.4529977694699525
2216	19	-1.4207980810086838
2216	20	-1.3853810810163694
2216	21	-1.6992368363076054
2216	22	-1.4646353797650704
2216	23	-1.5825313374506678
2216	24	-1.5311049794185578
2216	25	-1.621540544461027
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62405.0
3	1.0
4	1032.0
5	8491.0
6	9391.0
7	7334.0
8	6044.0
9	5216.0
10	4868.0
11	4329.0
12	4123.0
13	3791.0
14	3779.0
15	3724.0
16	3910.0
17	4083.0
18	4636.0
19	5511.0
20	7012.0
21	9177.0
22	11513.0
23	13700.0
24	16150.0
25	18048.0
26	20244.0
27	22190.0
28	25356.0
29	30244.0
30	38777.0
31	49380.0
32	62767.0
33	81863.0
34	108694.0
35	145821.0
36	209017.0
37	341123.0
38	1272657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.6855318803204	19.273635936074957	13.607816197003928	27.43301598660072
2	13.84688493828364	20.199199758739596	39.74635672421986	26.207558578756903
3	17.087117056845997	24.194380549455865	29.77129930612644	28.947203087571694
4	11.298181539882643	16.98532902975735	37.35795362601153	34.35853580434848
5	12.780097154711335	38.004464483048224	36.03469615146014	13.180742210780302
6	32.53601201310868	37.35240986061998	17.474473863046434	12.637104263224904
7	28.278393663564238	31.296930160494245	21.97494112722651	18.449735048715006
8	25.25378767973057	37.08392262950617	19.997113495967653	17.66517619479561
9	26.08911034348308	14.914106383497217	19.734094564462477	39.26268870855723
10	14.29824684984699	29.113342301586908	34.71479468651918	21.873616162046925
11	34.61752413283425	22.33436554433234	24.19542941081731	18.8526809120161
12	22.851004092672824	24.737882752862188	31.56787558335532	20.843237571109665
13	28.760269281042767	21.393610495883912	25.82122836535624	24.024891857717083
14	20.805293998941504	21.57498753031751	27.561158557248216	30.058559913492765
15	23.24766857764675	30.364784356996516	23.009471897094162	23.378075168262576
16	22.56905933252386	28.203004796297293	26.85720878114195	22.370727090036898
17	21.985356450024486	28.501644790709047	27.35687959705484	22.15611916221163
18	21.910904245696393	27.6598491724135	28.729833065907183	21.69941351598292
19	23.329034675207634	27.145245528005812	27.34228322331586	22.18343657347069
20	23.528394940452735	27.09586236069816	27.697369898960595	21.678372799888518
21	23.15853279642276	26.684026402841322	27.050804452052706	23.10663634868321
22	23.155887586006543	27.59870073054399	26.885138168267652	22.360273515181813
23	22.399875413037005	27.36039368261852	27.286448730865505	22.95328217347897
24	22.91059133772794	26.94824590761274	27.766133199005026	22.375029555654297
25	22.79403197839779	27.010300799802618	27.38122706459569	22.814440157203897
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	95.0
1	95.0
2	212.0
3	329.0
4	329.0
5	329.0
6	841.5
7	1354.0
8	1354.0
9	1354.0
10	2185.0
11	3016.0
12	3016.0
13	3016.0
14	5936.5
15	8857.0
16	8857.0
17	8857.0
18	17680.0
19	26503.0
20	26503.0
21	26503.0
22	43492.0
23	60481.0
24	60481.0
25	60481.0
26	87531.0
27	114581.0
28	114581.0
29	114581.0
30	147486.0
31	180391.0
32	180391.0
33	180391.0
34	211272.5
35	242154.0
36	242154.0
37	242154.0
38	267043.5
39	291933.0
40	291933.0
41	291933.0
42	310179.0
43	328425.0
44	328425.0
45	328425.0
46	341725.0
47	355025.0
48	355025.0
49	355025.0
50	343659.5
51	332294.0
52	332294.0
53	332294.0
54	296790.0
55	261286.0
56	261286.0
57	261286.0
58	224552.0
59	187818.0
60	187818.0
61	187818.0
62	152908.0
63	117998.0
64	117998.0
65	117998.0
66	91076.0
67	64154.0
68	64154.0
69	64154.0
70	47186.0
71	30218.0
72	30218.0
73	30218.0
74	21214.5
75	12211.0
76	12211.0
77	12211.0
78	8516.0
79	4821.0
80	4821.0
81	4821.0
82	3270.0
83	1719.0
84	1719.0
85	1719.0
86	1134.5
87	550.0
88	550.0
89	550.0
90	360.5
91	171.0
92	171.0
93	171.0
94	93.0
95	15.0
96	15.0
97	15.0
98	8.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18154120410401914
2	0.00734845897484809
3	0.004949739205856227
4	0.0022083451841512394
5	0.08121379789301024
6	0.3272158364240647
7	0.03137373158173486
8	0.014734992866664305
9	0.004911664288888102
10	0.003807491696812482
11	0.0
12	0.0
13	0.0
14	0.0011803224260118696
15	0.0
16	0.0
17	0.02037008057794678
18	0.028708487393966114
19	0.0
20	0.0
21	6.472735884581219E-4
22	7.614983393624965E-5
23	0.004683214787079353
24	0.0
25	1.1422475090437447E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2626401.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.25776466424041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18397572849435	57.038046612399604
2	12.609191149989288	17.717871688447403
3	3.320102747129569	6.997889924067819
4	1.232765956936352	3.464455219540853
5	0.5525862886288472	1.9411738711585782
6	0.30827533121284845	1.299524140328584
7	0.17672702374627813	0.8691511950924345
8	0.11582572333634217	0.6510125129784116
9	0.08514388283023785	0.5383816994237859
>10	0.37902858746734075	4.627945966576485
>50	0.021528979788089226	1.0394430138754256
>100	0.012244813100217114	1.661906050163836
>500	0.0016816126572549476	0.8209265483629647
>1k	9.221746829791462E-4	1.332271557583892
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3150	0.11993598844959319	No Hit
GTCCTACAGTGGACATTTCTAAATT	3139	0.11951716436294382	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3015	0.11479587465889633	No Hit
CCCATGTACTCTGCGTTGATACCAC	3009	0.11456742515708758	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2953	0.11243522980687261	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2719	0.1035256992363314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.807491696812482E-5	0.0	0.0	0.0	0.0
2	3.807491696812482E-5	0.0	0.0	0.0	0.0
3	3.807491696812482E-5	0.0	0.0	0.0	0.0
4	3.807491696812482E-5	0.0	0.0	7.614983393624964E-5	0.0
5	3.807491696812482E-5	7.614983393624964E-5	0.0	7.614983393624964E-5	0.0
6	1.903745848406241E-4	7.614983393624964E-5	0.0	7.614983393624964E-5	0.0
7	1.903745848406241E-4	7.614983393624964E-5	0.0	7.614983393624964E-5	0.0
8	1.903745848406241E-4	7.614983393624964E-5	0.0	7.614983393624964E-5	0.0
9	1.903745848406241E-4	7.614983393624964E-5	0.0	2.6652441877687377E-4	0.0
10	1.903745848406241E-4	7.614983393624964E-5	0.0	2.6652441877687377E-4	0.0
11	1.903745848406241E-4	7.614983393624964E-5	0.0	2.6652441877687377E-4	0.0
12	1.903745848406241E-4	7.614983393624964E-5	0.0	2.6652441877687377E-4	0.0
13	1.903745848406241E-4	7.614983393624964E-5	0.0	2.6652441877687377E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	620	0.0	13.667842	4
TTGCGCG	65	5.506225E-5	13.14082	18
GGACCGT	75	1.4476267E-5	12.693526	6
GTAGGAC	1710	0.0	10.690919	3
TTTGCGC	90	9.612269E-5	10.545505	17
ATAGGAC	275	0.0	10.387199	3
AGGACCT	1270	0.0	10.3461075	5
GTCCTAT	305	0.0	10.3058	1
GTCCTAA	870	0.0	10.181958	1
TAGGACA	565	0.0	10.111239	4
GTCCTAC	1680	0.0	10.035328	1
GACCGTG	135	6.5367203E-7	9.8419075	7
GGCGAGG	775	0.0	9.794129	19
TAGACAG	370	0.0	9.778753	5
TGGCGAG	1655	0.0	9.748645	18
CTACACT	470	0.0	9.724001	4
TGTAGGA	1930	0.0	9.719496	2
AGGACGT	1655	0.0	9.607714	5
GGACGTG	1670	0.0	9.577151	6
CCTACAC	480	0.0	9.5216	3
>>END_MODULE