FastQCFastQC Report
Thu 26 May 2016
SRR1513208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3223437
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32500.10082405829553982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10050.014.0328094
GTATTAA4150.011.7383721
GTAGGAC20750.011.7107473
TACCGAC658.116776E-411.6769057
TGTAGGA24150.010.9379672
GTCCTAC20300.010.9163581
GGACCGT1408.545612E-910.8421676
AGGACCT16950.010.7727975
GGACCTG16850.010.7536926
CTGTAGG23450.010.34611
TGGCGAG17750.010.157351518
TCCTACA22800.010.0798992
GGACGTG15550.010.0664826
GGCGAGG7550.010.0546819
AGGACGT16000.010.0453185
TAGGACG16700.010.0425774
GTATAAA5850.09.7967311
TTAGGAC11100.09.7868833
CTTACAC4600.09.7364923
ATAGGAC4050.09.6469783
Produced by FastQC (version 0.11.5)