##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513208_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3223437 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.486335858277982 31.0 30.0 34.0 25.0 34.0 2 29.306189945700815 31.0 30.0 34.0 22.0 34.0 3 29.51103247868657 31.0 30.0 34.0 23.0 34.0 4 32.59309922917681 37.0 33.0 37.0 22.0 37.0 5 32.73567096239201 37.0 33.0 37.0 25.0 37.0 6 33.09087380953932 37.0 35.0 37.0 27.0 37.0 7 33.041206947739326 37.0 35.0 37.0 26.0 37.0 8 32.92851884494718 37.0 35.0 37.0 26.0 37.0 9 34.17840832626789 39.0 35.0 39.0 25.0 39.0 10 33.75591643329775 39.0 34.0 39.0 19.0 39.0 11 34.22358122711876 39.0 35.0 39.0 26.0 39.0 12 34.21339582563581 39.0 35.0 39.0 25.0 39.0 13 34.25845487285776 39.0 35.0 39.0 26.0 39.0 14 34.487753599651555 40.0 34.0 41.0 17.0 41.0 15 34.92258263462261 40.0 34.0 41.0 22.0 41.0 16 34.7687493194376 40.0 34.0 41.0 18.0 41.0 17 34.3737259329095 39.0 34.0 41.0 17.0 41.0 18 34.110025727197396 39.0 33.0 41.0 17.0 41.0 19 34.74247674144089 39.0 34.0 41.0 19.0 41.0 20 34.951921815130866 40.0 35.0 41.0 22.0 41.0 21 35.00073927301821 40.0 35.0 41.0 23.0 41.0 22 34.68975537601634 40.0 35.0 41.0 18.0 41.0 23 34.228782197387446 39.0 34.0 41.0 10.0 41.0 24 34.47444296258931 40.0 34.0 41.0 17.0 41.0 25 34.11812422578757 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9532701788799756 1101 2 1.1715772532495947 1101 3 1.0790597405798223 1101 4 1.468384852430603 1101 5 1.3161061991471144 1101 6 1.0626918141547392 1101 7 0.9624434735861342 1101 8 1.0730369841169747 1101 9 1.4216589037705418 1101 10 1.761155658204295 1101 11 1.357822309885215 1101 12 1.2720863812265506 1101 13 1.1784901787296036 1101 14 2.1657826564126026 1101 15 1.7465208737298212 1101 16 1.760662224292588 1101 17 2.209415055441731 1101 18 2.2270647565104724 1101 19 1.817993444723541 1101 20 1.4995704948462105 1101 21 1.416096297803783 1101 22 1.6333647476165396 1101 23 1.9236556928168795 1101 24 1.5164370126802496 1101 25 1.7876363226912986 1102 1 0.9268919719783355 1102 2 1.1091306851426488 1102 3 1.0357934106427678 1102 4 1.2828997957952168 1102 5 1.2613430208903296 1102 6 0.9515042933506592 1102 7 0.9693134076015326 1102 8 0.9630130863267965 1102 9 1.3031649476015943 1102 10 1.5467768470512837 1102 11 1.1976642559961803 1102 12 1.0583848058694656 1102 13 1.0752231813055815 1102 14 2.048058242461771 1102 15 1.561143756764146 1102 16 1.6684002426818552 1102 17 2.0879969793675386 1102 18 2.279917077652513 1102 19 1.571133048745672 1102 20 1.3690510582403448 1102 21 1.352635412979545 1102 22 1.536880471765258 1102 23 1.7843694296109547 1102 24 1.5477994150712604 1102 25 1.8536217135093125 1103 1 0.9673429966627758 1103 2 1.2878737065822996 1103 3 1.0757344946215497 1103 4 1.5350617264638515 1103 5 1.3766649346899484 1103 6 1.119644993749425 1103 7 1.0835782186767844 1103 8 1.066735113028166 1103 9 1.4422141845123804 1103 10 1.7328576973220606 1103 11 1.3202961044344832 1103 12 1.1560019270458497 1103 13 1.257772365304561 1103 14 2.1657520343128667 1103 15 1.789818167207315 1103 16 1.7727037761263773 1103 17 2.2181855388783163 1103 18 2.427616739487732 1103 19 1.8675973203821101 1103 20 1.5824048449902932 1103 21 1.6227836932764248 1103 22 1.7645458969660197 1103 23 2.139308296873125 1103 24 1.8937813699694885 1103 25 2.148670093371031 1104 1 0.6989540547525301 1104 2 0.8734041475293175 1104 3 0.7193596922005305 1104 4 1.0861124078940065 1104 5 1.0329168518068244 1104 6 0.7822186185872013 1104 7 0.7128708240134856 1104 8 0.8469605448329887 1104 9 1.0284089671243777 1104 10 1.1655950343417842 1104 11 0.7997924990252159 1104 12 0.7634195763333338 1104 13 0.6870003268623961 1104 14 1.6781262876241598 1104 15 1.2765598075423412 1104 16 1.3819398984004465 1104 17 1.8365950033763951 1104 18 1.8461192868857594 1104 19 1.1981970985120967 1104 20 1.0021991031351973 1104 21 1.0022459971610331 1104 22 1.3506753196440968 1104 23 1.6471426880019848 1104 24 1.3833962588847797 1104 25 1.6209742635764073 1105 1 0.9171424078529888 1105 2 1.099871151965118 1105 3 1.0000240143399033 1105 4 1.347768060016847 1105 5 1.2619580646018491 1105 6 0.9322432619285621 1105 7 0.9251700647220602 1105 8 1.0832362388814971 1105 9 1.33081614863071 1105 10 1.6573959656063337 1105 11 1.1943786151912477 1105 12 1.1485376172729218 1105 13 1.140707920682594 1105 14 1.9167681202832725 1105 15 1.5257489216044533 1105 16 1.7780020818009206 1105 17 2.0261294986228435 1105 18 1.7653077616250172 1105 19 1.5794254030223058 1105 20 1.409909714610798 1105 21 1.412173457417282 1105 22 1.5499447827206652 1105 23 1.731679889743809 1105 24 1.6243124750878835 1105 25 1.8245405000648063 1106 1 0.49983029274145707 1106 2 0.712546229842598 1106 3 0.585037468350869 1106 4 1.0748953442275777 1106 5 0.9322468334632745 1106 6 0.5674193736338964 1106 7 0.5757260308534597 1106 8 0.6368340337198077 1106 9 0.8043719612975124 1106 10 0.7502484206915483 1106 11 0.6673909056577187 1106 12 0.5867383536074513 1106 13 0.602264532969599 1106 14 1.645333749829021 1106 15 1.1287992573583878 1106 16 1.2719810556737343 1106 17 1.558154308802763 1106 18 1.628532261751566 1106 19 1.1026490714425208 1106 20 0.8198828445855995 1106 21 0.8136036545633516 1106 22 1.0212265751702532 1106 23 1.3301488745913446 1106 24 1.028707895299064 1106 25 1.3000687655201375 1107 1 0.5870501070241794 1107 2 0.655073105319449 1107 3 0.7481673984352959 1107 4 1.0055058952898364 1107 5 0.9179720186789808 1107 6 0.658874913747006 1107 7 0.5850457764984611 1107 8 0.6183348980389738 1107 9 0.8669254011129723 1107 10 1.1659694143859554 1107 11 0.7512429761735149 1107 12 0.6316694224370565 1107 13 0.6025627262033098 1107 14 1.4500776195730225 1107 15 0.8719411667857315 1107 16 1.037371410346367 1107 17 1.4959000195694756 1107 18 1.5241540574958634 1107 19 0.9818625805450338 1107 20 0.7878119625055433 1107 21 0.6805323017175908 1107 22 0.9419172479739402 1107 23 1.2461375512402455 1107 24 1.0409718204527323 1107 25 1.262004673954337 1108 1 0.7043078893074117 1108 2 0.9724389613450235 1108 3 0.753548638959451 1108 4 1.2435461525938152 1108 5 1.1801818098167374 1108 6 0.8215821774974401 1108 7 0.6847493329300178 1108 8 0.8005416489837884 1108 9 1.0379257731322369 1108 10 1.275751903600863 1108 11 0.9858624387558663 1108 12 0.7348180357162661 1108 13 0.8242357862977912 1108 14 1.7884124719025039 1108 15 1.2545906272587644 1108 16 1.3856799770554957 1108 17 1.7508620369636034 1108 18 1.986584898058041 1108 19 1.3228917667273024 1108 20 1.077896955868944 1108 21 1.0779150620081168 1108 22 1.3191147446506832 1108 23 1.6614504352874206 1108 24 1.3080139834064113 1108 25 1.7271339745812213 1109 1 0.5355336669858666 1109 2 0.7092917725351917 1109 3 0.6101315233322815 1109 4 0.9052044561047623 1109 5 0.9853368572512835 1109 6 0.5883954629052468 1109 7 0.5010895345043025 1109 8 0.5421456971159841 1109 9 0.726625256269223 1109 10 1.1247101328248377 1109 11 0.6749328652325701 1109 12 0.5798155764143473 1109 13 0.5660332525450613 1109 14 1.5308676908164216 1109 15 1.0256849166646944 1109 16 1.1022093040259904 1109 17 1.5226202231948562 1109 18 1.6368734769026716 1109 19 1.0490488264966942 1109 20 0.9154417140168292 1109 21 0.8132481360306443 1109 22 1.04251487946172 1109 23 1.3788528062445167 1109 24 1.1327095034791341 1109 25 1.402266336143498 1110 1 0.5480506597794879 1110 2 0.7593634909220768 1110 3 0.6416595909306899 1110 4 0.982444034618311 1110 5 0.9074185445265286 1110 6 0.5862699788631573 1110 7 0.5937836759495099 1110 8 0.5245873188052386 1110 9 0.7187765569755271 1110 10 1.0628258975754932 1110 11 0.6480917457349449 1110 12 0.7025761282386256 1110 13 0.5472949910952067 1110 14 1.3343232234614817 1110 15 1.0877581417568152 1110 16 1.237426078634094 1110 17 1.5485489779342672 1110 18 1.1278534478894713 1110 19 0.891477404628354 1110 20 0.7105553816319272 1110 21 0.5312376555663292 1110 22 0.8377014547963313 1110 23 -0.19974863959294709 1110 24 0.4510816296930642 1110 25 0.9027356337660066 1111 1 0.12249061097965352 1111 2 0.5027529701632041 1111 3 0.4245912686396238 1111 4 0.7893381791436127 1111 5 0.4947447014387052 1111 6 0.2855624823299152 1111 7 0.17310013730494234 1111 8 0.30664772880465563 1111 9 0.527205484010679 1111 10 0.7468630859777079 1111 11 0.39064610879862727 1111 12 0.2930975122645094 1111 13 0.17638295582393937 1111 14 1.0389530507171472 1111 15 0.6680205418901792 1111 16 0.6909596777732787 1111 17 1.0599027691406988 1111 18 1.3096486098357047 1111 19 0.8362223926683541 1111 20 0.5484642254648833 1111 21 0.39293010295874353 1111 22 0.7201513541403628 1111 23 1.071203620177755 1111 24 0.7473464344558494 1111 25 1.0007446473375126 1112 1 -0.09046027288882286 1112 2 0.1135710881467844 1112 3 0.040934338323801 1112 4 0.4470964287472796 1112 5 0.04088291176977066 1112 6 -0.04386321823275807 1112 7 -0.23071996185906585 1112 8 -0.17261149963658795 1112 9 0.060313119790016856 1112 10 0.33975130951089483 1112 11 -0.18409248523238375 1112 12 -0.08325727244371706 1112 13 -0.1654747577186484 1112 14 0.38975165776381004 1112 15 0.16888988411414374 1112 16 0.25330813737141256 1112 17 0.6724150290951414 1112 18 0.7181392834950344 1112 19 0.23778688743286835 1112 20 0.05461492649374122 1112 21 -0.06270034037092387 1112 22 0.0973248664020403 1112 23 0.5344931757386391 1112 24 0.21067990568673878 1112 25 0.5340688057824323 1113 1 -0.04127599577178387 1113 2 0.11031085212592373 1113 3 0.07620711694315574 1113 4 0.23196577243933092 1113 5 0.34694645305902583 1113 6 0.14854834907765024 1113 7 0.11238728174677703 1113 8 0.10696415447976904 1113 9 0.2839966681124082 1113 10 0.4661067232589815 1113 11 0.07506781516474348 1113 12 0.004031003228853081 1113 13 -0.1921339810039484 1113 14 0.43819918959754034 1113 15 0.197417227568792 1113 16 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-4.628385841911271 2215 18 -3.0776151941155163 2215 19 -3.5568050427076408 2215 20 -3.408367748457927 2215 21 -3.466619272642344 2215 22 -9.475852125754802 2215 23 -10.547583555601001 2215 24 -6.776274051407995 2215 25 -5.079080109840689 2216 1 -1.9863542557322909 2216 2 -1.988123806623605 2216 3 -1.772597982986479 2216 4 -6.82071734098243 2216 5 -3.542499723172682 2216 6 -2.8420376439845327 2216 7 -2.2463444486709037 2216 8 -8.041090873080314 2216 9 -4.12685782662599 2216 10 -2.4050160373686857 2216 11 -2.4144785847236747 2216 12 -2.595070250788403 2216 13 -2.33047167450194 2216 14 -1.8115769548427068 2216 15 -2.3521017244462925 2216 16 -2.007255878724159 2216 17 -1.7394094162279856 2216 18 -7.692977202095747 2216 19 -4.997879176239927 2216 20 -4.299076310218108 2216 21 -3.4586465040090424 2216 22 -3.19453152437325 2216 23 -2.417136649438639 2216 24 -2.814118057243288 2216 25 -2.3171390089953725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 147742.0 3 6.0 4 1229.0 5 7519.0 6 8755.0 7 7902.0 8 7217.0 9 6824.0 10 6512.0 11 6227.0 12 6024.0 13 6083.0 14 5999.0 15 6186.0 16 6179.0 17 6624.0 18 7601.0 19 9132.0 20 11766.0 21 15266.0 22 18639.0 23 22386.0 24 25887.0 25 29357.0 26 33514.0 27 37454.0 28 41749.0 29 50379.0 30 60013.0 31 72621.0 32 92380.0 33 120968.0 34 158692.0 35 212620.0 36 302910.0 37 440617.0 38 1222458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91317696104448 18.14732894735207 12.327865979394257 25.611628112209196 2 14.657331138275284 19.717732040144305 41.1742897034961 24.450647118084316 3 18.61116839305637 24.28727522797955 29.39537325693114 27.70618312203294 4 11.527401591342636 16.24714022994094 38.63788150914207 33.587576669574354 5 12.956852582689965 37.733035210822976 36.01978456926761 13.290327637219448 6 32.57491228222597 36.598821658936416 17.866531216605498 12.959734842232121 7 28.675046465373597 31.14737668446676 21.98068157488915 18.196895275270492 8 25.527572922536173 36.24832137298658 20.417782648519704 17.80632305595754 9 26.52963009620713 15.00345347795949 19.781168026441293 38.68574839939209 10 14.099613529418153 28.54098434414034 35.1474747537433 22.211927372698202 11 34.953580449177416 22.25808153209341 24.053301807756036 18.735036210973135 12 23.35611877142341 24.348973445055215 31.322447049302315 20.972460734219055 13 29.065042353355174 21.34648200436241 25.586006775419694 24.002468866862724 14 20.772223559989772 21.060033215251213 28.345760090559967 29.821983134199048 15 23.43799511683865 29.811988666993784 23.126023985030848 23.623992231136718 16 22.7567732610637 27.62488883703525 27.00616182597077 22.61217607593028 17 21.993841925371285 27.868437803663497 27.829208863381798 22.308511407583413 18 22.014895150836754 27.098697830275718 29.049888731690228 21.8365182871973 19 23.801875293247306 26.7081267672417 27.066695298361292 22.423302641149704 20 24.01065275428377 26.845946240333063 27.14714535789971 21.996255647483455 21 23.694160933694484 26.438508442427388 26.53924406696343 23.328086556914695 22 23.720745600395283 27.038225397067777 26.681024235302736 22.560004767234204 23 22.815234506158934 26.86839366275725 27.312369316245842 23.00400251483798 24 23.45459562321008 26.566742989063147 27.245202226416488 22.733459161310286 25 23.26625159283703 26.549901031519084 27.272442321144496 22.91140505449939 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 204.0 1 204.0 2 349.0 3 494.0 4 494.0 5 494.0 6 1046.5 7 1599.0 8 1599.0 9 1599.0 10 2712.0 11 3825.0 12 3825.0 13 3825.0 14 7506.0 15 11187.0 16 11187.0 17 11187.0 18 21196.0 19 31205.0 20 31205.0 21 31205.0 22 50883.0 23 70561.0 24 70561.0 25 70561.0 26 102044.5 27 133528.0 28 133528.0 29 133528.0 30 173649.5 31 213771.0 32 213771.0 33 213771.0 34 251149.5 35 288528.0 36 288528.0 37 288528.0 38 321009.0 39 353490.0 40 353490.0 41 353490.0 42 376847.5 43 400205.0 44 400205.0 45 400205.0 46 414957.0 47 429709.0 48 429709.0 49 429709.0 50 419195.0 51 408681.0 52 408681.0 53 408681.0 54 369473.0 55 330265.0 56 330265.0 57 330265.0 58 284814.0 59 239363.0 60 239363.0 61 239363.0 62 196631.5 63 153900.0 64 153900.0 65 153900.0 66 119860.0 67 85820.0 68 85820.0 69 85820.0 70 63193.5 71 40567.0 72 40567.0 73 40567.0 74 28612.0 75 16657.0 76 16657.0 77 16657.0 78 11677.0 79 6697.0 80 6697.0 81 6697.0 82 4456.5 83 2216.0 84 2216.0 85 2216.0 86 1461.0 87 706.0 88 706.0 89 706.0 90 454.0 91 202.0 92 202.0 93 202.0 94 124.0 95 46.0 96 46.0 97 46.0 98 28.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16457588592548886 2 0.25860595383126767 3 0.2589161817029463 4 0.4560349713675186 5 0.25451094592511037 6 0.0186446950878829 7 0.018830831810890052 8 0.017527874749839998 9 0.018582649513547187 10 0.021157540848479435 11 0.020381971169282975 12 0.02826175910991901 13 0.02810664517407972 14 0.025469708264811753 15 0.0416946259536017 16 0.021436745932990157 17 0.041136215784580245 18 0.032449835377579894 19 0.027951531238240426 20 0.030526422573172673 21 0.03539700015852644 22 0.04476588188321968 23 0.029874944042647643 24 0.048953958150880564 25 0.03719632181426223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3223437.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.47973333933241 #Duplication Level Percentage of deduplicated Percentage of total 1 81.81112059974016 57.66025964061651 2 11.936298639915496 16.825342903997605 3 3.127901448929781 6.613609800968473 4 1.2457596821707801 3.5120324081715233 5 0.611625338816821 2.1553595391694187 6 0.3164810005702869 1.3383297916295356 7 0.21085301019525524 1.0402604752649987 8 0.145739242253752 0.8217330344896646 9 0.10711489593948965 0.6794486372237013 >10 0.45437975158088806 5.383954325193521 >50 0.019991907130567435 0.9537045568581349 >100 0.010644902021678362 1.3696710514443389 >500 0.00151161159690812 0.7715856685879778 >1k 5.779691382149257E-4 0.8747081663847457 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3250 0.10082405829553982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.1022787167858405E-5 0.0 4 0.0 0.0 0.0 3.1022787167858405E-5 0.0 5 0.0 0.0 0.0 3.1022787167858405E-5 0.0 6 0.0 0.0 0.0 3.1022787167858405E-5 0.0 7 0.0 0.0 0.0 6.204557433571681E-5 0.0 8 0.0 0.0 0.0 6.204557433571681E-5 0.0 9 0.0 0.0 0.0 6.204557433571681E-5 0.0 10 0.0 0.0 0.0 6.204557433571681E-5 0.0 11 0.0 0.0 0.0 6.204557433571681E-5 0.0 12 0.0 0.0 0.0 9.306836150357523E-5 3.1022787167858405E-5 13 0.0 0.0 0.0 9.306836150357523E-5 3.1022787167858405E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1005 0.0 14.032809 4 GTATTAA 415 0.0 11.738372 1 GTAGGAC 2075 0.0 11.710747 3 TACCGAC 65 8.116776E-4 11.676905 7 TGTAGGA 2415 0.0 10.937967 2 GTCCTAC 2030 0.0 10.916358 1 GGACCGT 140 8.545612E-9 10.842167 6 AGGACCT 1695 0.0 10.772797 5 GGACCTG 1685 0.0 10.753692 6 CTGTAGG 2345 0.0 10.3461 1 TGGCGAG 1775 0.0 10.1573515 18 TCCTACA 2280 0.0 10.079899 2 GGACGTG 1555 0.0 10.066482 6 GGCGAGG 755 0.0 10.05468 19 AGGACGT 1600 0.0 10.045318 5 TAGGACG 1670 0.0 10.042577 4 GTATAAA 585 0.0 9.796731 1 TTAGGAC 1110 0.0 9.786883 3 CTTACAC 460 0.0 9.736492 3 ATAGGAC 405 0.0 9.646978 3 >>END_MODULE