FastQCFastQC Report
Thu 26 May 2016
SRR1513208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3223437
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33940.10529133964771144No Hit
GTCCTACAGTGGACATTTCTAAATT33460.10380224586365423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9100.012.7623234
TGGCGAG17750.011.33564718
ATAACGC600.005787207311.106533
GTAGGAC20050.010.9680443
AAACCGT700.001467877910.878516
AGGACCT16600.010.8383955
GTCTTAT2650.010.7824281
GGCGAGA11500.010.72621519
TATGGCG19950.010.46683916
TAGGACT4950.010.3848424
TGTAGGA22600.010.36331752
TCCAACG1106.1199116E-610.35374418
GTCCTAC19600.010.350581
GCACCGT1752.2737368E-1010.3345856
GGACCTG15650.010.2789766
ACCGTGC1856.184564E-1110.2593528
AGGACGT17400.010.175955
ATGGCGA20300.010.09950717
ACCGGGT856.6618586E-410.0481298
GTCCTAA10450.010.0257661
Produced by FastQC (version 0.11.5)