##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513208_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3223437 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.80012421523982 31.0 31.0 34.0 27.0 34.0 2 30.907058831923813 33.0 31.0 34.0 27.0 34.0 3 31.002858439609646 33.0 31.0 34.0 27.0 34.0 4 34.393993119766264 37.0 35.0 37.0 31.0 37.0 5 34.132641959498514 37.0 35.0 37.0 30.0 37.0 6 34.049803362063535 37.0 35.0 37.0 30.0 37.0 7 33.91641158179918 37.0 35.0 37.0 29.0 37.0 8 33.88831765596784 37.0 35.0 37.0 29.0 37.0 9 35.45194523733518 39.0 37.0 39.0 30.0 39.0 10 35.29561924120124 39.0 35.0 39.0 29.0 39.0 11 35.30655942709598 39.0 35.0 39.0 29.0 39.0 12 35.24053487007812 39.0 35.0 39.0 29.0 39.0 13 35.21129403180518 39.0 35.0 39.0 29.0 39.0 14 36.381060960707465 40.0 37.0 41.0 28.0 41.0 15 36.360434840203176 40.0 37.0 41.0 28.0 41.0 16 36.3225814557567 40.0 37.0 41.0 28.0 41.0 17 36.256613049983606 40.0 37.0 41.0 27.0 41.0 18 36.20941032816835 40.0 36.0 41.0 27.0 41.0 19 36.28728807170731 40.0 37.0 41.0 27.0 41.0 20 36.25036754247097 40.0 37.0 41.0 27.0 41.0 21 36.19577115979 40.0 37.0 41.0 27.0 41.0 22 36.137062086214186 40.0 37.0 41.0 27.0 41.0 23 36.05519077928311 40.0 37.0 41.0 27.0 41.0 24 35.9915447393574 40.0 37.0 41.0 27.0 41.0 25 35.93825968989002 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36265819999353255 1101 2 0.4557308432526277 1101 3 0.36447060382578655 1101 4 0.551365281102342 1101 5 0.4396917520923651 1101 6 0.4505354813512312 1101 7 0.41284169466181453 1101 8 0.4510633589878026 1101 9 0.5122349575058323 1101 10 0.6012897364396181 1101 11 0.6055174847697913 1101 12 0.5280447658024343 1101 13 0.5822617432597141 1101 14 0.7105491767703569 1101 15 0.7577587193584137 1101 16 0.6968867869881876 1101 17 0.6990954950639505 1101 18 0.6470889671377194 1101 19 0.7150698975198111 1101 20 0.657468527195924 1101 21 0.6652889875456864 1101 22 0.7280449768909136 1101 23 0.667575165690927 1101 24 0.6587388321870975 1101 25 0.5942863565146226 1102 1 0.3918289616049506 1102 2 0.46944989622913624 1102 3 0.3488586983173505 1102 4 0.53777515850431 1102 5 0.4624515648095766 1102 6 0.4463250437871764 1102 7 0.42050795820147613 1102 8 0.5006081065240267 1102 9 0.5034415192540891 1102 10 0.47922472496498614 1102 11 0.5825295847277232 1102 12 0.49888875916621345 1102 13 0.5030239255556737 1102 14 0.5734570135065553 1102 15 0.509415887509796 1102 16 0.5479099303424277 1102 17 0.7263349358152595 1102 18 0.6239284865822299 1102 19 0.6291541361323496 1102 20 0.6311245729152688 1102 21 0.6396097975938275 1102 22 0.6927355308084913 1102 23 0.7036044437774223 1102 24 0.6724126541384621 1102 25 0.679693246912386 1103 1 0.47800379787040015 1103 2 0.4485425016356217 1103 3 0.5137046587414176 1103 4 0.5336971147379757 1103 5 0.49272922883135095 1103 6 0.45221969683659324 1103 7 0.4726395888035668 1103 8 0.4383167136780912 1103 9 0.5087354011336913 1103 10 0.5406061569517107 1103 11 0.6005865415306033 1103 12 0.49210698362764305 1103 13 0.5526046322585287 1103 14 0.6511854882991841 1103 15 0.6130857869390312 1103 16 0.6563250677535777 1103 17 0.7006289555967768 1103 18 0.6179351578270271 1103 19 0.5749415987737478 1103 20 0.6208696217396934 1103 21 0.6175711191579012 1103 22 0.6433371823887555 1103 23 0.598085769574439 1103 24 0.62347238066031 1103 25 0.6701482901039313 1104 1 0.21797877040843971 1104 2 0.30210354811589823 1104 3 0.31838126339682304 1104 4 0.42770780650147344 1104 5 0.36130347370409055 1104 6 0.3670120865070814 1104 7 0.2910157218924425 1104 8 0.3773494418776622 1104 9 0.2962421452866053 1104 10 0.48382481142752454 1104 11 0.44967856477992996 1104 12 0.4521007936320487 1104 13 0.43619544058963555 1104 14 0.6028706917333793 1104 15 0.48224555327920626 1104 16 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1107 15 0.2134880505448038 1107 16 0.2005829004919235 1107 17 0.2738841607838367 1107 18 0.26097620815424705 1107 19 0.20421260155423226 1107 20 0.2859876763288014 1107 21 0.2906547510070894 1107 22 0.3564334090837491 1107 23 0.1575957362742244 1107 24 0.2434514560878398 1107 25 0.25178734309795203 1108 1 0.4079540991476698 1108 2 0.4225895278703433 1108 3 0.4303275072355035 1108 4 0.5284011713419687 1108 5 0.4186050287863381 1108 6 0.4603418703121491 1108 7 0.4345967522926202 1108 8 0.5131965038622113 1108 9 0.6377191122116272 1108 10 0.6430526239286252 1108 11 0.6195396253038865 1108 12 0.6347566660005555 1108 13 0.6602690782437577 1108 14 0.6223781096216925 1108 15 0.6231192233165572 1108 16 0.541553246892363 1108 17 0.5701198506683127 1108 18 0.6113610021720959 1108 19 0.5943742434300674 1108 20 0.6712945823201579 1108 21 0.6481377848489629 1108 22 0.49345632408411433 1108 23 0.53816286264491 1108 24 0.5926328816643434 1108 25 0.6209832071151666 1109 1 0.15850535306395486 1109 2 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-0.9736156174785222 2215 20 -0.8236670749906452 2215 21 -0.9130111358893345 2215 22 -0.9303209785549242 2215 23 -0.7964347091106916 2215 24 -0.8965079398891618 2215 25 -0.9337114490850169 2216 1 -0.44908699710582667 2216 2 -0.3409286808877674 2216 3 -0.40394090131967175 2216 4 -0.28997134257468105 2216 5 -0.4232002089793667 2216 6 -0.5931534691008551 2216 7 -0.7580358498631696 2216 8 -0.7492476696405745 2216 9 -0.7959909017266256 2216 10 -0.7841211306600826 2216 11 -0.7236274592032146 2216 12 -0.802257850185164 2216 13 -0.8098840927819495 2216 14 -0.9540296457886726 2216 15 -1.0016705360108134 2216 16 -0.9762004579242571 2216 17 -1.0762698869939626 2216 18 -1.0605183463310226 2216 19 -1.0342295222620024 2216 20 -1.1300850977855887 2216 21 -1.2631164509351933 2216 22 -1.0819898989591294 2216 23 -1.1882170047255656 2216 24 -1.1585824664377427 2216 25 -1.2199171098519983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76343.0 3 2.0 4 1215.0 5 10673.0 6 12104.0 7 9321.0 8 7372.0 9 6464.0 10 5963.0 11 5515.0 12 4901.0 13 4771.0 14 4714.0 15 4727.0 16 4851.0 17 4894.0 18 5474.0 19 6565.0 20 8304.0 21 10586.0 22 13522.0 23 16567.0 24 19009.0 25 21753.0 26 23779.0 27 26196.0 28 29566.0 29 35919.0 30 45154.0 31 57919.0 32 74065.0 33 97174.0 34 128283.0 35 173190.0 36 248571.0 37 405870.0 38 1612141.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.190588307687335 18.891631685904404 13.31526257248531 27.60251743392295 2 14.051318804226254 19.987465814093824 39.57430305737958 26.386912324300344 3 17.144955282891903 24.20953549244973 29.587284686356654 29.058224538301715 4 11.428464175935451 16.693770855540983 37.21503373494275 34.66273123358082 5 13.038437943187548 37.83930624927041 35.745474053891606 13.376781753650437 6 32.69393734091476 36.99970715400058 17.448424404497192 12.857931100587466 7 28.36801721874773 31.19721933112265 22.057051996968845 18.37771145316077 8 25.613379820000166 36.52062998390011 19.958591413039333 17.907398783060394 9 26.371654454982025 14.784726981437228 19.428290259701377 39.41532830387937 10 14.534677375320786 28.806023121559438 34.44466437006408 22.214635133055687 11 34.9639530724503 22.129081474215255 23.935414279850978 18.97155117348346 12 23.11101473365231 24.483711020255708 31.352590418239913 21.05268382785207 13 28.93461234080269 21.25914047645417 25.611110128722853 24.19513705402029 14 21.25693487676449 21.157008743304004 27.18102514099326 30.405031238938246 15 23.375204789173793 29.895046808732417 22.996943945236094 23.732804456857696 16 22.94252997654367 27.72934603654422 26.482416129119322 22.845707857792785 17 22.321994296867 28.03964887566363 27.117745804101418 22.52061102336795 18 22.32852711504016 27.289346257492646 28.29201748212253 22.09010914534466 19 23.73922617380144 26.725976031174177 26.949712372228774 22.585085422795608 20 23.936717236912028 26.77328578160516 27.196436598574753 22.093560382908056 21 23.52368162009948 26.349387464878 26.628812309460148 23.498118605562375 22 23.615939900100297 27.079576339883598 26.514402501928846 22.790081258087262 23 22.881686950106367 26.911797053707186 26.921693936871538 23.284822059314905 24 23.33776028506219 26.58265075445867 27.260684790799385 22.818904169679755 25 23.19940138262744 26.593769117845973 27.021140227112255 23.185689272414336 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 101.0 1 101.0 2 238.0 3 375.0 4 375.0 5 375.0 6 942.5 7 1510.0 8 1510.0 9 1510.0 10 2456.0 11 3402.0 12 3402.0 13 3402.0 14 6683.5 15 9965.0 16 9965.0 17 9965.0 18 19634.5 19 29304.0 20 29304.0 21 29304.0 22 48633.0 23 67962.0 24 67962.0 25 67962.0 26 98898.5 27 129835.0 28 129835.0 29 129835.0 30 169092.0 31 208349.0 32 208349.0 33 208349.0 34 246116.5 35 283884.0 36 283884.0 37 283884.0 38 316617.5 39 349351.0 40 349351.0 41 349351.0 42 372859.5 43 396368.0 44 396368.0 45 396368.0 46 414661.0 47 432954.0 48 432954.0 49 432954.0 50 423316.0 51 413678.0 52 413678.0 53 413678.0 54 374501.5 55 335325.0 56 335325.0 57 335325.0 58 290285.5 59 245246.0 60 245246.0 61 245246.0 62 201966.5 63 158687.0 64 158687.0 65 158687.0 66 123509.0 67 88331.0 68 88331.0 69 88331.0 70 65060.0 71 41789.0 72 41789.0 73 41789.0 74 29385.5 75 16982.0 76 16982.0 77 16982.0 78 11894.5 79 6807.0 80 6807.0 81 6807.0 82 4548.5 83 2290.0 84 2290.0 85 2290.0 86 1506.0 87 722.0 88 722.0 89 722.0 90 460.5 91 199.0 92 199.0 93 199.0 94 108.0 95 17.0 96 17.0 97 17.0 98 10.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17732625145147865 2 0.007817742366300319 3 0.005397964967207363 4 0.002264663463253664 5 0.07609889692275666 6 0.3146951530307557 7 0.02801357681257614 8 0.013463889630850549 9 0.004901600372521628 10 0.003877848395982301 11 0.0 12 0.0 13 0.0 14 0.0017372760814000708 15 0.0 16 0.0 17 0.02007174329760439 18 0.029316533873626194 19 0.0 20 0.0 21 9.306836150357522E-4 22 1.2409114867143365E-4 23 0.006204557433571681 24 0.0 25 2.7920508451072565E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3223437.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.65916833749984 #Duplication Level Percentage of deduplicated Percentage of total 1 80.04665996219073 54.15890441236472 2 12.76169128759863 17.268908381976818 3 3.6387098890090517 7.385762547353663 4 1.3710529296116178 3.710572038568591 5 0.6987324699908054 2.363782890499248 6 0.38687138734925436 1.5705237792975186 7 0.24208880139692687 1.146566887643677 8 0.1694873488032888 0.9173898451006616 9 0.11347425438338805 0.690981631136814 >10 0.5328785715191272 6.150184841755677 >50 0.022815424335434657 1.0519763390169932 >100 0.013107655684043403 1.6068991862093323 >500 0.0015130301579572155 0.7200048795681162 >1k 9.169879699364356E-4 1.257542339508185 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3394 0.10529133964771144 No Hit GTCCTACAGTGGACATTTCTAAATT 3346 0.10380224586365423 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.1022787167858405E-5 2 0.0 0.0 0.0 0.0 3.1022787167858405E-5 3 0.0 0.0 0.0 0.0 3.1022787167858405E-5 4 0.0 0.0 0.0 0.0 3.1022787167858405E-5 5 0.0 0.0 0.0 0.0 3.1022787167858405E-5 6 0.0 0.0 0.0 0.0 3.1022787167858405E-5 7 0.0 0.0 0.0 0.0 3.1022787167858405E-5 8 0.0 0.0 0.0 0.0 3.1022787167858405E-5 9 0.0 0.0 0.0 0.0 3.1022787167858405E-5 10 0.0 0.0 0.0 0.0 3.1022787167858405E-5 11 0.0 0.0 0.0 0.0 3.1022787167858405E-5 12 0.0 0.0 0.0 3.1022787167858405E-5 6.204557433571681E-5 13 0.0 0.0 0.0 3.1022787167858405E-5 6.204557433571681E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 910 0.0 12.762323 4 TGGCGAG 1775 0.0 11.335647 18 ATAACGC 60 0.0057872073 11.10653 3 GTAGGAC 2005 0.0 10.968044 3 AAACCGT 70 0.0014678779 10.87851 6 AGGACCT 1660 0.0 10.838395 5 GTCTTAT 265 0.0 10.782428 1 GGCGAGA 1150 0.0 10.726215 19 TATGGCG 1995 0.0 10.466839 16 TAGGACT 495 0.0 10.384842 4 TGTAGGA 2260 0.0 10.3633175 2 TCCAACG 110 6.1199116E-6 10.353744 18 GTCCTAC 1960 0.0 10.35058 1 GCACCGT 175 2.2737368E-10 10.334585 6 GGACCTG 1565 0.0 10.278976 6 ACCGTGC 185 6.184564E-11 10.259352 8 AGGACGT 1740 0.0 10.17595 5 ATGGCGA 2030 0.0 10.099507 17 ACCGGGT 85 6.6618586E-4 10.048129 8 GTCCTAA 1045 0.0 10.025766 1 >>END_MODULE