FastQCFastQC Report
Thu 26 May 2016
SRR1513207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862724
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30350.10601790462510532No Hit
GTATCAACGCAGAGTACTTTTTTTT29470.10294390936744163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7400.012.2337494
GGATCGT550.003099565612.0751986
CGTGCGC1201.0202712E-811.86102110
GTCCTAC15850.011.0876771
TGTAGGA19250.010.9977182
GTAGGAC17600.010.9915453
GGCGAGG5550.010.77127619
TCCAACG1502.1773303E-910.7541818
AGGACCT12700.010.6357485
CTGTAGG19400.010.6341811
TGGCGAG13300.010.63051318
GTCCTAA9800.010.623131
TCCTACA17800.010.3935062
TATACTG4450.010.2604085
GGACGTG15100.010.2416416
TAGGACT5400.010.2353334
TAGACGT750.002581586610.1647444
AGGACGT15700.09.9969545
GTATTAG2700.09.9048141
TCTATAC2700.09.8825643
Produced by FastQC (version 0.11.5)