FastQCFastQC Report
Thu 26 May 2016
SRR1513207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862724
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40400.1411243277381962No Hit
GTCCTACAGTGGACATTTCTAAATT31100.10863778694697777No Hit
TATCAACGCAGAGTACTTTTTTTTT29650.10357268112469102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGCG801.3040153E-714.2371317
TAGGACC7400.011.8355074
GGCGAGG8300.011.66048819
TTAGGAC13500.011.1421583
ACCGCCC600.005926599711.0710048
CAGTCCG600.00593199611.0696519
TAGACGT700.001466289510.8798454
GTACCGT1053.3853212E-610.8792736
TGGCGAG17250.010.78445518
GGACGTG17450.010.5285816
AGGACGT18050.010.49558455
GACGTGA9900.010.450157
GCCGGTT1002.429973E-510.438374511
ACCGTGT1002.429973E-510.43837458
TAGGACG19350.010.2332394
CGGAAGT750.002603229110.1545224
GACCGTG1502.4818291E-810.1241837
TATGGCG19000.010.09123116
GTAGGAC17100.010.0769353
GACGGTG856.6515664E-410.049747
Produced by FastQC (version 0.11.5)