FastQCFastQC Report
Thu 26 May 2016
SRR1513206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61023
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24804.064041427002933No Hit
CCCATGTACTCTGCGTTGATACCAC20573.3708601674778365No Hit
GAGTACATGGGAAGCAGTGGTATCA10091.6534749192927256No Hit
GTATCAACGCAGAGTACTTTTTTTT8461.3863625190501943No Hit
CATGTACTCTGCGTTGATACCACTG7681.2585418612654244No Hit
TATCAACGCAGAGTACATGGGAAGC7361.2061026170460318No Hit
GCGTTGATACCACTGCTTCCCATGT6661.0913917703161102No Hit
GTATCAACGCAGAGTACATGGGAAG6521.068449600970126No Hit
ACGCAGAGTACATGGGAAGCAGTGG6010.9848745554954689No Hit
GGTATCAACGCAGAGTACATGGGAA5840.9570162070039165No Hit
GGTATCAACGCAGAGTACTTTTTTT5630.9226029529849401No Hit
ACTCTGCGTTGATACCACTGCTTCC5110.8373891811284271No Hit
TATCAACGCAGAGTACTTTTTTTTT5080.8324730019828589No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3180.5211149894302148No Hit
GTACTCTGCGTTGATACCACTGCTT2970.4867017354112384No Hit
GGGAAGCAGTGGTATCAACGCAGAG2960.4850630090293824No Hit
GTGGTATCAACGCAGAGTACATGGG2930.4801468298838143No Hit
ATACCACTGCTTCCCATGTACTCTG2610.42770758566442163No Hit
GCTTCCCATGTACTCTGCGTTGATA2590.4244301329007096No Hit
GATACCACTGCTTCCCATGTACTCT2550.4178752273732855No Hit
CTGCTTCCCATGTACTCTGCGTTGA2280.3736296150631729No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2270.3719908886813169No Hit
CATGGGAAGCAGTGGTATCAACGCA2220.3637972567720368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2220.3637972567720368No Hit
CAGTGGTATCAACGCAGAGTACATG2210.36215853039018076No Hit
TACCACTGCTTCCCATGTACTCTGC2080.34085508742605247No Hit
GTTGATACCACTGCTTCCCATGTAC2020.3310227291349163No Hit
GCAGTGGTATCAACGCAGAGTACAT2020.3310227291349163No Hit
GCAGAGTACATGGGAAGCAGTGGTA2020.3310227291349163No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1940.31791291808006816No Hit
GAACAAAAAAAAAAAAAAAAAAAAA1910.3129967389345001No Hit
ACATGGGAAGCAGTGGTATCAACGC1900.31135801255264406No Hit
CCACTGCTTCCCATGTACTCTGCGT1900.31135801255264406No Hit
ATGGGAAGCAGTGGTATCAACGCAG1710.2802222112973797No Hit
CTTCCCATGTACTCTGCGTTGATAC1700.27858348491552365No Hit
AAGCAGTGGTATCAACGCAGAGTAC1650.2703898530062436No Hit
CCATGTACTCTGCGTTGATACCACT1390.22778296707798698No Hit
ATCAACGCAGAGTACATGGGAAGCA1330.21795060878685088No Hit
TTCCCATGTACTCTGCGTTGATACC1270.20811825049571472No Hit
TGATACCACTGCTTCCCATGTACTC1230.20156334496829065No Hit
CCCATGTACTCTGCGTTTATACCAC1220.19992461858643462No Hit
GTACATGGGAAGCAGTGTTATCAAC1210.19828589220457862No Hit
GTACATGGGAAGCAGTTGTATCAAC1070.1753437228585943No Hit
GCAGAGTACTTTTTTTTTTTTTTTT970.15895645904003408No Hit
GGAAGCAGTGGTATCAACGCAGAGT970.15895645904003408No Hit
AAAAAGTACTCTGCGTTGATACCAC970.15895645904003408No Hit
GTACATGGTAAGCAGTGGTATCAAC790.1294593841666257No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA770.12618193140291367No Hit
GTGGTATCAACGCAGAGTACTTTTT760.12454320502105762No Hit
ATCAACGCAGAGTACTTTTTTTTTT760.12454320502105762No Hit
CTGCGTTGATACCACTGCTTCCCAT700.11471084672992152No Hit
GTACTGGTTCACTATCGGTCAGTCA650.1065172148206414No Hit
AAAGTACTCTGCGTTGATACCACTG630.10323976205692935No Hit
GACTTACTCCTTAGATTTAAGGAAT620.10160103567507332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT350.002153079316.26885214
TGCGTTT350.002153079316.26885212
GTTTATA350.002153079316.26885215
GAACAAA602.3621069E-514.3292071
GCGTTTA400.00523845914.23524613
CCATGTA5500.09.5292182
ATGTACT6000.08.7279344
GGAAGCA8700.08.6175058
TGTACTC6100.08.5777985
CCCATGT5800.08.5645841
GAAGCAG8750.08.3513449
CATGTAC6350.08.2468663
AAGCAGT8700.08.18117510
GTACTCT6500.08.1761416
GGGAAGC9300.08.1635817
TACTCTG6400.08.155617
CTGCGTT6300.07.98378911
AGCAGTG8450.07.973984211
TGGGAAG9800.07.94074966
CTCTGCG6600.07.908479
Produced by FastQC (version 0.11.5)