##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513206_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61023
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.941644953542106	31.0	27.0	33.0	7.0	34.0
2	26.634744276748112	31.0	26.0	33.0	7.0	34.0
3	26.871687724300674	31.0	26.0	34.0	7.0	34.0
4	29.48429280762991	35.0	28.0	37.0	2.0	37.0
5	29.947101912393688	35.0	30.0	37.0	2.0	37.0
6	29.830260721367353	35.0	30.0	37.0	2.0	37.0
7	29.68826508037953	35.0	30.0	37.0	2.0	37.0
8	29.217082083804467	35.0	29.0	37.0	2.0	37.0
9	29.915032037100765	37.0	27.0	39.0	2.0	39.0
10	29.63499008570539	37.0	27.0	39.0	2.0	39.0
11	29.991396686495257	37.0	27.0	39.0	2.0	39.0
12	30.030562247021614	37.0	29.0	39.0	2.0	39.0
13	29.877390492109534	37.0	27.0	39.0	2.0	39.0
14	29.995493502449897	38.0	26.0	40.0	2.0	41.0
15	30.25768972354686	37.0	27.0	40.0	2.0	41.0
16	30.012683742195566	37.0	27.0	40.0	2.0	41.0
17	29.854317224653	37.0	27.0	40.0	2.0	41.0
18	29.670763482621307	37.0	25.0	40.0	2.0	41.0
19	30.150877537977482	37.0	27.0	40.0	2.0	41.0
20	30.46356291889943	38.0	28.0	40.0	2.0	41.0
21	30.485816823165035	38.0	29.0	40.0	2.0	41.0
22	30.275256870360355	38.0	27.0	40.0	2.0	41.0
23	29.886911492388116	38.0	27.0	40.0	2.0	41.0
24	30.07241531881422	38.0	28.0	40.0	2.0	41.0
25	29.719859725021713	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.279910168553286
1101	2	1.2459826668306313
1101	3	0.9374143774293806
1101	4	1.2117413877715926
1101	5	1.331622179312788
1101	6	1.3354942800743927
1101	7	1.1930436706706935
1101	8	1.523564259864056
1101	9	1.268403666856372
1101	10	1.6636072532844928
1101	11	1.1865537756493474
1101	12	1.8332089057984078
1101	13	1.1633413121005134
1101	14	2.4791609782964947
1101	15	2.333795382352548
1101	16	1.8323259445550377
1101	17	2.03655303195125
1101	18	1.7607787111558721
1101	19	1.8158532734484858
1101	20	1.408520946559637
1101	21	0.7639213644382394
1101	22	1.0812657113669317
1101	23	1.5051168891067235
1101	24	1.0972552172788106
1101	25	1.021341706396747
1102	1	2.661228849871968
1102	2	2.83487155571952
1102	3	2.6029821429971456
1102	4	2.720166296196499
1102	5	2.90207394976456
1102	6	2.860891105471218
1102	7	2.838587016214042
1102	8	2.922343258643057
1102	9	3.3723108707635774
1102	10	4.192056581733819
1102	11	3.0352717243672984
1102	12	2.832842605432109
1102	13	3.4773828261420263
1102	14	4.1649973641328835
1102	15	2.8312312797884474
1102	16	3.0004578126869035
1102	17	3.059752055150277
1102	18	4.02280557318273
1102	19	3.0842293418245568
1102	20	2.1981424361811257
1102	21	3.157938458455334
1102	22	3.1321814622826807
1102	23	4.056643140632975
1102	24	3.5457289657525592
1102	25	4.0761646612197
1103	1	0.8199245020458825
1103	2	0.9959826668306313
1103	3	0.8189241719826548
1103	4	1.5187170208341811
1103	5	-0.18295020482481306
1103	6	0.9954321682731511
1103	7	0.05066431090002865
1103	8	0.9486717610585131
1103	9	1.188494445642803
1103	10	1.4075155189318878
1103	11	0.8454166519035304
1103	12	1.0183498518089174
1103	13	1.0952572222772936
1103	14	1.6017123399782953
1103	15	1.2928254826870003
1103	16	2.0265447692086482
1103	17	2.068206161430467
1103	18	1.7218393896078545
1103	19	1.9120071196023325
1103	20	1.6251955762777435
1103	21	0.8023829028997795
1103	22	1.846674215905871
1103	23	2.139493382178866
1103	24	2.3650526372501446
1103	25	1.630754033200379
1104	1	0.6242723281328395
1104	2	0.47424353639584993
1104	3	0.5906633024174361
1104	4	1.1491518034428765
1104	5	0.7844410995230149
1104	6	0.7019539074035848
1104	7	0.9637077891608996
1104	8	0.5573674132324271
1104	9	1.047190097816717
1104	10	1.0596894319753645
1104	11	1.0954166519035304
1104	12	1.0400889822437023
1104	13	0.7474311353207739
1104	14	1.7756253834565534
1104	15	1.531955917469606
1104	16	1.678718682252125
1104	17	2.0899452918652486
1104	18	2.1674915635208976
1104	19	1.0859201630805941
1104	20	0.5273694893212237
1104	21	1.6719481202910842
1104	22	1.2162394332971722
1104	23	1.4329716430484325
1104	24	0.5498352459457969
1104	25	0.9024931636351603
1105	1	1.0264462411763162
1105	2	1.256852232048022
1105	3	1.4276198241565652
1105	4	1.7904561512689625
1105	5	1.3387889256099719
1105	6	1.6258669508818464
1105	7	1.3876208326391613
1105	8	0.7747587175802515
1105	9	1.1450161847732403
1105	10	2.1792546493666727
1105	11	0.7041123040774409
1105	12	1.0944368083306593
1105	13	1.616996352712075
1105	14	2.8408427747609046
1105	15	1.944999395730477
1105	16	2.0917621605129924
1105	17	1.9377713788217719
1105	18	2.5914046069991556
1105	19	1.966354945689286
1105	20	2.1578042719299226
1105	21	2.215426381160647
1105	22	2.6184133463406525
1105	23	2.824275990874522
1105	24	2.2672265502936213
1105	25	2.435101859287336
1106	1	1.6924116455708926
1106	2	1.7728643872607392
1106	3	1.5094337559003712
1106	4	1.9266179090997255
1106	5	2.1952639139222647
1106	6	1.8669842954353761
1106	7	1.9005941846728192
1106	8	2.0743145848079294
1106	9	2.3683682184338295
1106	10	1.5332752197266508
1106	11	1.8449491437221397
1106	12	1.9737028204858724
1106	13	1.5770244032029588
1106	14	3.0011980809787637
1106	15	2.9946721400035017
1106	16	2.63594168365465
1106	17	3.1099312666198102
1106	18	3.2428772577705445
1106	19	3.135125399172228
1106	20	2.5146298913782594
1106	21	2.227114085695476
1106	22	1.789170709594508
1106	23	2.4591521012064526
1106	24	1.9711769944263935
1106	25	1.9905015787824247
1107	1	-0.7129284534988187
1107	2	-0.18098362530420076
1107	3	-0.4693025011976104
1107	4	0.0040614272826431375
1107	5	-0.4921832287622827
1107	6	-0.725500904516295
1107	7	-0.860913608005685
1107	8	-1.06317484372898
1107	9	-0.442795246589732
1107	10	0.33912274902470685
1107	11	0.02665749215756108
1107	12	-0.3810150724705146
1107	13	-0.13410281680429037
1107	14	0.0909649047071639
1107	15	0.5982724782903226
1107	16	-0.33325005248163464
1107	17	0.4879667867357931
1107	18	0.09970944334503073
1107	19	0.4709958836472765
1107	20	-0.050671546340719686
1107	21	-0.30435867013392937
1107	22	0.4876121738931687
1107	23	0.5419115551148721
1107	24	0.3188875175627963
1107	25	1.188399367836432
1108	1	1.1208033179570762
1108	2	1.7672592625753119
1108	3	1.4405707954794167
1108	4	2.2726485657000453
1108	5	1.7675585833342993
1108	6	1.9433970156367053
1108	7	1.349461720705765
1108	8	1.6334543697541655
1108	9	1.2678191449952543
1108	10	0.5818910971002502
1108	11	1.429597965502051
1108	12	1.2385163642973538
1108	13	1.335775261130209
1108	14	2.285801146638793
1108	15	2.234777379079226
1108	16	2.147266323325205
1108	17	2.278428178081718
1108	18	2.1464462351212674
1108	19	1.2790283961980755
1108	20	0.6830124125404637
1108	21	0.8662126901338212
1108	22	0.41374174597062563
1108	23	0.6337116985525952
1108	24	0.649511471662727
1108	25	0.9064247085010244
1109	1	1.2528955165386364
1109	2	1.8084826668306313
1109	3	1.0883988096638113
1109	4	2.0680829628631656
1109	5	1.8659628386534486
1109	6	0.8831133276934402
1109	7	1.5108092384362628
1109	8	1.3001210364208333
1109	9	1.7188386485413538
1109	10	1.8010843595115986
1109	11	1.8331883910339641
1109	12	1.2932592720987728
1109	13	0.5558550483642506
1109	14	1.9622195863551077
1109	15	1.8145646131217816
1109	16	1.2108744793535742
1109	17	1.7194742773724947
1109	18	1.907527795404956
1109	19	0.8703404529356646
1109	20	1.5300868806255714
1109	21	1.2190495695664474
1109	22	1.5613481289493478
1109	23	1.5552542517440848
1109	24	1.588784521308117
1109	25	1.4622757723308126
1110	1	-1.2367909521082296
1110	2	-0.9718391153475849
1110	3	-0.9240805632734812
1110	4	-0.5663023506681846
1110	5	-0.593195577188137
1110	6	-1.6036853521745442
1110	7	-1.470213863873969
1110	8	-1.4655555312359319
1110	9	-1.142650625386036
1110	10	-0.3522778517095233
1110	11	-1.396081410946234
1110	12	-1.4407547543038675
1110	13	-1.5360385159921854
1110	14	-0.41443057866139554
1110	15	-0.9700888522247517
1110	16	-0.21043327642200538
1110	17	-0.42759667972321225
1110	18	-0.3760943168062667
1110	19	-0.3973988209917252
1110	20	-0.7168190599684863
1110	21	-0.6382111565061628
1110	22	-0.5902607819417405
1110	23	-2.4490774314242323
1110	24	-1.0970653136753832
1110	25	-0.22955591083750448
1111	1	-1.0485750716966606
1111	2	-0.6265663527772105
1111	3	-0.6597629550420692
1111	4	-0.5072846841956569
1111	5	-0.5874685338955707
1111	6	-0.6757102017183243
1111	7	-0.7489946831323664
1111	8	-0.7096828851477959
1111	9	-0.4950093906743298
1111	10	0.28944220264885345
1111	11	-0.38433611876995855
1111	12	-0.9867652377051463
1111	13	-1.2964733830082977
1111	14	-0.393172570507641
1111	15	-0.3962197006037087
1111	16	-0.06424806966603569
1111	17	0.33099388521563355
1111	18	0.6391454424637786
1111	19	-0.23995366471139334
1111	20	-1.0397660605508996
1111	21	-0.7466367049433593
1111	22	-0.7658577534035942
1111	23	0.6030483693911428
1111	24	-0.1575390081036474
1111	25	0.2637463605661061
1112	1	-1.8506523382468423
1112	2	-1.0411460460406552
1112	3	-1.5280409593130848
1112	4	-1.25937165759888
1112	5	-1.5634926068911064
1112	6	-1.9997249561349406
1112	7	-1.777144556943277
1112	8	-2.2378327589587066
1112	9	-1.112947655089009
1112	10	-1.0354461685412062
1112	11	-1.950536856490789
1112	12	-1.6684775265810927
1112	13	-1.0211870308436701
1112	14	-0.8896781034138712
1112	15	-0.5542472680663373
1112	16	-0.5668689199863621
1112	17	-0.09096301635687709
1112	18	-0.1483715445290379
1112	19	-0.5855176328729144
1112	20	-0.40988836689918173
1112	21	-1.1332606614566565
1112	22	-0.14471622748629542
1112	23	-0.08274079776086651
1112	24	-0.45350095723973993
1112	25	0.10707775252883067
1113	1	-1.548771150128033
1113	2	-1.5240173331693683
1113	3	-1.4536845236695193
1113	4	-1.9865003704701678
1113	5	-1.7090371613465507
1113	6	-1.480220005639893
1113	7	-2.065857428230405
1113	8	-1.8965456302458357
1113	9	-1.893244684791977
1113	10	-1.3768323071550697
1113	11	-1.3480616089660344
1113	12	-2.02382406123456
1113	13	-2.160394951635748
1113	14	-2.5961137469782294
1113	15	-2.365435386878218
1113	16	-1.649542187313095
1113	17	-1.8013590559608375
1113	18	-1.7516388712617115
1113	19	-2.137992880397668
1113	20	-2.727413119374429
1113	21	-2.5676170971002215
1113	22	-2.6011518710506536
1113	23	-1.8222457482559165
1113	24	-2.12538214535855
1113	25	-1.842724227669187
1114	1	0.07122884987196798
1114	2	-0.044017333169367845
1114	3	0.8963154763304786
1114	4	1.0134996295298322
1114	5	-2.99903716134655
1114	6	0.21977999436010975
1114	7	0.6141425717695945
1114	8	1.4534543697541658
1114	9	1.2067553152080208
1114	10	1.05316769284493
1114	11	1.761938391033965
1114	12	0.3161759387654399
1114	13	2.249605048364252
1114	14	-3.6861137469782292
1114	15	-0.2154353868782195
1114	16	0.4104578126869072
1114	17	1.3386409440391631
1114	18	-5.291638871261711
1114	19	-0.8179928803976679
1114	20	0.3125868806255703
1114	21	1.3123829028997775
1114	22	0.7988481289493485
1114	23	1.687754251744085
1114	24	1.954617854641448
1114	25	2.2872757723308155
1115	1	-1.9527496447516874
1115	2	-1.8615442148898005
1115	3	-1.4583081795835007
1115	4	-5.148650908104575
1115	5	-2.3316178065078432
1115	6	-1.90720925295172
1115	7	-1.4972552776927692
1115	8	-0.49557788831035054
1115	9	-1.2337823192005821
1115	10	-1.3376925222088332
1115	11	-1.090534727245604
1115	12	-1.2951143838152035
1115	13	-0.9283519408830614
1115	14	-5.988049230849196
1115	15	-2.8225321610717664
1115	16	-2.28878949914105
1115	17	-1.2126493785414816
1115	18	-0.5098109142724638
1115	19	-0.6068100846987434
1115	20	-0.4638647322776528
1115	21	-0.6976170971002205
1115	22	-1.6301841291151682
1115	23	-0.5623532751376352
1115	24	-0.4804359087994108
1115	25	-0.2675629373466073
1116	1	0.2794106680537851
1116	2	0.7005281213760846
1116	3	-0.2705027054877043
1116	4	0.7944087204389234
1116	5	0.025053747744358645
1116	6	0.674779994360108
1116	7	0.5486880263150482
1116	8	0.576636187935982
1116	9	0.8258462242989317
1116	10	0.5236222382994775
1116	11	0.9951202092157807
1116	12	0.1502668478563507
1116	13	0.9677868665460672
1116	14	0.9811589802944987
1116	15	2.0532009767581485
1116	16	1.620912358141453
1116	17	1.1531863985846158
1116	18	1.5874520378291983
1116	19	0.07109802869324255
1116	20	0.9334959715346614
1116	21	-0.027162551645673716
1116	22	0.43066631076753126
1116	23	-0.09815483916500511
1116	24	-0.469927599904004
1116	25	0.43954849960353926
1201	1	1.2112288498719685
1201	2	1.416437212285178
1201	3	1.252224567239569
1201	4	2.2375905386207435
1201	5	0.9114173841079953
1201	6	0.924779994360108
1201	7	1.310051662678685
1201	8	1.2925452788450755
1201	9	1.2917553152080217
1201	10	0.9440767837540207
1201	11	0.3133020273976008
1201	12	0.2525395751290773
1201	13	0.09278686654606716
1201	14	0.6743407984763188
1201	15	0.31456461312178163
1201	16	0.6095487217778164
1201	17	1.0736409440391625
1201	18	0.6783611287382882
1201	19	0.2870071196023325
1201	20	0.9334959715346614
1201	21	0.8478374483543263
1201	22	1.1011208562220745
1201	23	1.7768451608349949
1201	24	1.4846178546414492
1201	25	1.485003045058086
1202	1	-0.45366476714930926
1202	2	-0.19018754593532705
1202	3	-0.016876013031222215
1202	4	0.017329416763875116
1202	5	0.00160113652578886
1202	6	-0.056602984363294695
1202	7	-0.16117657716657519
1202	8	-0.5686732898203033
1202	9	-0.16835106777070052
1202	10	-1.056406775240177
1202	11	-0.38955097066816435
1202	12	-0.28276023144732676
1202	13	-0.9408204835506417
1202	14	-0.746113746978228
1202	15	-0.43543538687821837
1202	16	-0.19315920858969093
1202	17	0.14013030574129104
1202	18	-0.32163887126171176
1202	19	-0.7954396889083064
1202	20	-0.7318812044808105
1202	21	-1.2401702885895816
1202	22	-0.6181731476463952
1202	23	-0.13224574825591517
1202	24	-0.563254485784082
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2211	12	-0.4671573945678915
2211	13	0.14691687632123873
2211	14	0.5173271132368242
2211	15	0.2957474088207057
2211	16	-4.976961542151802
2211	17	-0.7932945398318054
2211	18	0.5869632792759241
2211	19	1.1888888400324404
2211	20	1.4501137623460032
2211	21	1.3776517201040832
2211	22	1.8644395267988116
2211	23	2.018291886152685
2211	24	0.9604243062543532
2211	25	-5.708423152400371
2212	1	-2.338771150128032
2212	2	-4.134017333169368
2212	3	-1.623684523669521
2212	4	-3.7965003704701665
2212	5	-1.7890371613465526
2212	6	-1.3902200056398932
2212	7	-2.445857428230404
2212	8	-1.5465456302458342
2212	9	-0.7732446847919796
2212	10	-0.7168323071550695
2212	11	-0.7180616089660354
2212	12	-0.9638240612345577
2212	13	-0.5503949516357487
2212	14	-5.596113746978229
2212	15	-3.9654353868782195
2212	16	-1.9395421873130942
2212	17	-7.091359055960837
2212	18	-1.731638871261712
2212	19	-0.5779928803976659
2212	20	-1.4674131193744273
2212	21	-0.7976170971002219
2212	22	-0.971151871050651
2212	23	-0.2322457482559166
2212	24	-0.2953821453585519
2212	25	-0.4527242276691865
2213	1	0.2042195040775745
2213	2	-0.24934443597310718
2213	3	-0.18798358909008073
2213	4	0.5936865454176825
2213	5	-0.06511192770169316
2213	6	0.37688279809842484
2213	7	0.9352640670966963
2213	8	0.7923328744270641
2213	9	0.9074095208154986
2213	10	-2.9061781015475923
2213	11	-0.7588092725174391
2213	12	0.07178341540095445
2213	13	-0.13777812920584154
2213	14	1.6604283090965382
2213	15	0.022508538355427987
2213	16	-0.21543003778038283
2213	17	0.4626596356279471
2213	18	0.6456508483644576
2213	19	-0.2351891420799106
2213	20	0.12763360959753456
2213	21	0.5734109402829581
2213	22	0.43342756820168304
2213	23	0.07336172837959865
2213	24	0.8748047705292983
2213	25	1.442415959246702
2214	1	-2.0580019193588015
2214	2	-2.388632717784752
2214	3	-2.002146062131061
2214	4	-2.18111575508555
2214	5	-4.951344853654245
2214	6	-2.7963738517937387
2214	7	-2.8289343513073284
2214	8	-2.4050071687073746
2214	9	-2.5255523770996717
2214	10	-5.329524614847376
2214	11	-3.851907762812189
2214	12	-3.0376702150807127
2214	13	-3.8696257208665195
2214	14	-3.2172675931320747
2214	15	-3.0604353868782184
2214	16	-3.1553114180823236
2214	17	-2.8109744405762207
2214	18	-2.8024081020309417
2214	19	-3.7226082650130508
2214	20	-3.9843361962975052
2214	21	-3.0918478663309905
2214	22	-3.3953826402814222
2214	23	-4.564938055948222
2214	24	-4.448074453050857
2214	25	-3.6819549968999574
2215	1	-2.0484798879921087
2215	2	-4.423920245790729
2215	3	-3.089898115902532
2215	4	-1.7018401762954092
2215	5	-1.6692313361038345
2215	6	-2.610414180397175
2215	7	-2.8281875253177837
2215	8	-3.4733417467506875
2215	9	-7.287128179937611
2215	10	-4.013337161524003
2215	11	-3.3145664633349696
2215	12	-3.42508619715689
2215	13	-3.828550291441573
2215	14	-6.906307921735511
2215	15	-3.891746066489869
2215	16	-8.551969371779112
2215	17	-4.303786240426856
2215	18	-3.190570910096664
2215	19	-3.65838122991223
2215	20	-3.9389665174326822
2215	21	-4.1053840873914815
2215	22	-9.268142162312788
2215	23	-9.423507884178246
2215	24	-7.042081174484764
2215	25	-5.194277625727441
2216	1	-1.3093593854221481
2216	2	-1.601076156698781
2216	3	-1.469566876610699
2216	4	-6.0759121351760506
2216	5	-3.5266842201700825
2216	6	-1.8561023585810688
2216	7	-1.3646809576421681
2216	8	-6.566545630245834
2216	9	-3.671479978909627
2216	10	-1.8968323071550692
2216	11	-0.9392380795542721
2216	12	-1.0867652377051478
2216	13	-0.5356890692828067
2216	14	0.35388625302177346
2216	15	-0.5295530339370416
2216	16	-1.2760127755483879
2216	17	-1.9690061147843672
2216	18	-6.362815341849949
2216	19	-4.608581115691784
2216	20	-6.1397660605508975
2216	21	-4.074087685335513
2216	22	-3.7246812828153573
2216	23	-2.5793045717853254
2216	24	-3.135970380652669
2216	25	-2.5362536394338946
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6014.0
3	0.0
4	39.0
5	331.0
6	687.0
7	523.0
8	490.0
9	324.0
10	328.0
11	311.0
12	355.0
13	281.0
14	276.0
15	210.0
16	215.0
17	188.0
18	211.0
19	289.0
20	464.0
21	552.0
22	760.0
23	793.0
24	840.0
25	887.0
26	917.0
27	940.0
28	916.0
29	1047.0
30	1255.0
31	1449.0
32	1815.0
33	2246.0
34	2943.0
35	3752.0
36	5323.0
37	7672.0
38	15380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.704155997636406	17.40200906046878	10.992383953778479	20.90145098811634
2	13.714539152717775	24.735121638714126	36.08095011252197	25.469389096046125
3	22.177141167581063	32.80659680015769	26.009395840862055	19.00686619139919
4	12.447566251583293	20.160879077495025	30.517675313780003	36.87387935714168
5	10.568157553259637	43.460192835203095	31.46794566449303	14.503703947044233
6	30.072119324700868	32.14063268316669	19.270611375184394	18.51663661694804
7	24.661530896574334	36.41698082281593	19.880347484019012	19.04114079659072
8	29.80495000819538	31.60137682347156	20.53761678413375	18.056056384199312
9	30.947385674479595	15.902311096541549	17.1299786920177	36.02032453696115
10	17.934176883236084	33.05907034681702	27.88795646758015	21.118796302366746
11	28.515932336742722	29.494492525570415	16.53389719381065	25.455677943876214
12	26.03314591085684	34.03275248758258	24.48568103208039	15.44842056948019
13	31.0318826325711	23.89804114416851	20.69830341775264	24.371772805507746
14	22.491599049258255	30.371281042537497	24.653716908450125	22.48340299975412
15	33.36885420594455	24.983195894880076	21.765004836303426	19.882945062871947
16	17.835073513743875	30.51025258568408	30.877411529446476	20.77726237112557
17	23.860115095174855	32.04630039512731	25.388159296968503	18.705425212729327
18	23.742622950819673	25.79016393442623	30.30655737704918	20.160655737704918
19	21.65103927611304	32.060192774244314	30.873385351780215	15.415382597862434
20	24.45122051181167	27.51684453843506	27.87586681748881	20.156068132264462
21	21.18032786885246	29.142622950819675	30.854098360655733	18.822950819672133
22	22.553777544596013	29.190713536201468	22.760362014690454	25.49514690451207
23	18.368852459016395	30.703278688524588	28.468852459016393	22.459016393442624
24	20.923102147893097	35.80259058862109	24.438432529922938	18.83587473356288
25	21.196124399560635	26.535731265472073	24.007738085479613	28.26040624948768
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	260.0
1	260.0
2	272.5
3	285.0
4	285.0
5	285.0
6	550.5
7	816.0
8	816.0
9	816.0
10	761.5
11	707.0
12	707.0
13	707.0
14	620.5
15	534.0
16	534.0
17	534.0
18	613.0
19	692.0
20	692.0
21	692.0
22	960.0
23	1228.0
24	1228.0
25	1228.0
26	1679.0
27	2130.0
28	2130.0
29	2130.0
30	2573.5
31	3017.0
32	3017.0
33	3017.0
34	3192.0
35	3367.0
36	3367.0
37	3367.0
38	3576.0
39	3785.0
40	3785.0
41	3785.0
42	4712.5
43	5640.0
44	5640.0
45	5640.0
46	10413.0
47	15186.0
48	15186.0
49	15186.0
50	13148.5
51	11111.0
52	11111.0
53	11111.0
54	8281.0
55	5451.0
56	5451.0
57	5451.0
58	4135.0
59	2819.0
60	2819.0
61	2819.0
62	2364.0
63	1909.0
64	1909.0
65	1909.0
66	1504.5
67	1100.0
68	1100.0
69	1100.0
70	848.0
71	596.0
72	596.0
73	596.0
74	421.5
75	247.0
76	247.0
77	247.0
78	176.5
79	106.0
80	106.0
81	106.0
82	67.0
83	28.0
84	28.0
85	28.0
86	17.5
87	7.0
88	7.0
89	7.0
90	3.5
91	0.0
92	0.0
93	0.0
94	1.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16223391180374613
2	0.23925405175097914
3	0.2376153253691231
4	0.380184520590597
5	0.23269914622355506
6	0.02130344296412828
7	0.02130344296412828
8	0.02130344296412828
9	0.02130344296412828
10	0.018025990200416234
11	0.02458089572784032
12	0.03277452763712043
13	0.02949707487340839
14	0.02949707487340839
15	0.04260688592825656
16	0.022942169345984302
17	0.04916179145568064
18	0.037690706782688495
19	0.031135801255264405
20	0.039329433164544514
21	0.037690706782688495
22	0.05080051783753666
23	0.037690706782688495
24	0.05407797060124871
25	0.04260688592825656
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	61023.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.91686741064845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43844742686106	44.520918342264395
2	7.370216600688745	7.505366828900578
3	2.063016961153487	3.151270832309129
4	0.8882881143188183	1.8091539255690476
5	0.4698915387338676	1.1962702587548957
6	0.24781951015416304	0.7570915884174819
7	0.2413826397605484	0.8603313504744112
8	0.15448488944675098	0.6292709306327122
9	0.14161114865952174	0.6489356472149845
>10	0.7852981880209843	7.212034806548351
>50	0.06115026873933894	2.151647739376956
>100	0.09655305590421936	9.924126968520067
>500	0.03218435196807312	10.545204267243498
>1k	0.009655305590421936	9.088376513773495
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2480	4.064041427002933	No Hit
CCCATGTACTCTGCGTTGATACCAC	2057	3.3708601674778365	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1009	1.6534749192927256	No Hit
GTATCAACGCAGAGTACTTTTTTTT	846	1.3863625190501943	No Hit
CATGTACTCTGCGTTGATACCACTG	768	1.2585418612654244	No Hit
TATCAACGCAGAGTACATGGGAAGC	736	1.2061026170460318	No Hit
GCGTTGATACCACTGCTTCCCATGT	666	1.0913917703161102	No Hit
GTATCAACGCAGAGTACATGGGAAG	652	1.068449600970126	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	601	0.9848745554954689	No Hit
GGTATCAACGCAGAGTACATGGGAA	584	0.9570162070039165	No Hit
GGTATCAACGCAGAGTACTTTTTTT	563	0.9226029529849401	No Hit
ACTCTGCGTTGATACCACTGCTTCC	511	0.8373891811284271	No Hit
TATCAACGCAGAGTACTTTTTTTTT	508	0.8324730019828589	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	318	0.5211149894302148	No Hit
GTACTCTGCGTTGATACCACTGCTT	297	0.4867017354112384	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	296	0.4850630090293824	No Hit
GTGGTATCAACGCAGAGTACATGGG	293	0.4801468298838143	No Hit
ATACCACTGCTTCCCATGTACTCTG	261	0.42770758566442163	No Hit
GCTTCCCATGTACTCTGCGTTGATA	259	0.4244301329007096	No Hit
GATACCACTGCTTCCCATGTACTCT	255	0.4178752273732855	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	228	0.3736296150631729	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	227	0.3719908886813169	No Hit
CATGGGAAGCAGTGGTATCAACGCA	222	0.3637972567720368	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	222	0.3637972567720368	No Hit
CAGTGGTATCAACGCAGAGTACATG	221	0.36215853039018076	No Hit
TACCACTGCTTCCCATGTACTCTGC	208	0.34085508742605247	No Hit
GTTGATACCACTGCTTCCCATGTAC	202	0.3310227291349163	No Hit
GCAGTGGTATCAACGCAGAGTACAT	202	0.3310227291349163	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	202	0.3310227291349163	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	194	0.31791291808006816	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	191	0.3129967389345001	No Hit
ACATGGGAAGCAGTGGTATCAACGC	190	0.31135801255264406	No Hit
CCACTGCTTCCCATGTACTCTGCGT	190	0.31135801255264406	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	171	0.2802222112973797	No Hit
CTTCCCATGTACTCTGCGTTGATAC	170	0.27858348491552365	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	165	0.2703898530062436	No Hit
CCATGTACTCTGCGTTGATACCACT	139	0.22778296707798698	No Hit
ATCAACGCAGAGTACATGGGAAGCA	133	0.21795060878685088	No Hit
TTCCCATGTACTCTGCGTTGATACC	127	0.20811825049571472	No Hit
TGATACCACTGCTTCCCATGTACTC	123	0.20156334496829065	No Hit
CCCATGTACTCTGCGTTTATACCAC	122	0.19992461858643462	No Hit
GTACATGGGAAGCAGTGTTATCAAC	121	0.19828589220457862	No Hit
GTACATGGGAAGCAGTTGTATCAAC	107	0.1753437228585943	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	97	0.15895645904003408	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	97	0.15895645904003408	No Hit
AAAAAGTACTCTGCGTTGATACCAC	97	0.15895645904003408	No Hit
GTACATGGTAAGCAGTGGTATCAAC	79	0.1294593841666257	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	77	0.12618193140291367	No Hit
GTGGTATCAACGCAGAGTACTTTTT	76	0.12454320502105762	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	76	0.12454320502105762	No Hit
CTGCGTTGATACCACTGCTTCCCAT	70	0.11471084672992152	No Hit
GTACTGGTTCACTATCGGTCAGTCA	65	0.1065172148206414	No Hit
AAAGTACTCTGCGTTGATACCACTG	63	0.10323976205692935	No Hit
GACTTACTCCTTAGATTTAAGGAAT	62	0.10160103567507332	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAT	35	0.0021530793	16.268852	14
TGCGTTT	35	0.0021530793	16.268852	12
GTTTATA	35	0.0021530793	16.268852	15
GAACAAA	60	2.3621069E-5	14.329207	1
GCGTTTA	40	0.005238459	14.235246	13
CCATGTA	550	0.0	9.529218	2
ATGTACT	600	0.0	8.727934	4
GGAAGCA	870	0.0	8.617505	8
TGTACTC	610	0.0	8.577798	5
CCCATGT	580	0.0	8.564584	1
GAAGCAG	875	0.0	8.351344	9
CATGTAC	635	0.0	8.246866	3
AAGCAGT	870	0.0	8.181175	10
GTACTCT	650	0.0	8.176141	6
GGGAAGC	930	0.0	8.163581	7
TACTCTG	640	0.0	8.15561	7
CTGCGTT	630	0.0	7.983789	11
AGCAGTG	845	0.0	7.9739842	11
TGGGAAG	980	0.0	7.9407496	6
CTCTGCG	660	0.0	7.90847	9
>>END_MODULE