FastQCFastQC Report
Thu 26 May 2016
SRR1513206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61023
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC28724.706422168690494No Hit
CCCATGTACTCTGCGTTGATACCAC28674.6982285367812135No Hit
GTATCAACGCAGAGTACTTTTTTTT16322.674401455189027No Hit
TATCAACGCAGAGTACTTTTTTTTT12232.0041623650099143No Hit
GGTATCAACGCAGAGTACTTTTTTT11271.8468446323517362No Hit
GAGTACATGGGAAGCAGTGGTATCA11241.841928453206168No Hit
CATGTACTCTGCGTTGATACCACTG9471.5518738836176522No Hit
TATCAACGCAGAGTACATGGGAAGC8881.4551890270881471No Hit
ACGCAGAGTACATGGGAAGCAGTGG7891.292955115284401No Hit
GTATCAACGCAGAGTACATGGGAAG7341.2028251642823198No Hit
GCGTTGATACCACTGCTTCCCATGT7121.1667731838814872No Hit
GGTATCAACGCAGAGTACATGGGAA6871.1258050243350868No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6561.07500450649755No Hit
ACTCTGCGTTGATACCACTGCTTCC6491.063533421824558No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6241.0225652622781574No Hit
ATACCACTGCTTCCCATGTACTCTG3730.611244940432296No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3720.60960621405044No Hit
GTGGTATCAACGCAGAGTACATGGG3220.5276698949576389No Hit
GCTTCCCATGTACTCTGCGTTGATA3210.5260311685757829No Hit
GTACTCTGCGTTGATACCACTGCTT3040.49817282008423047No Hit
TACCACTGCTTCCCATGTACTCTGC3000.4916179145568064No Hit
ACATGGGAAGCAGTGGTATCAACGC2970.4867017354112384No Hit
GGGAAGCAGTGGTATCAACGCAGAG2890.47359192435639025No Hit
CTGCTTCCCATGTACTCTGCGTTGA2850.4670370188289661No Hit
CATGGGAAGCAGTGGTATCAACGCA2840.4653982924471101No Hit
CAGTGGTATCAACGCAGAGTACATG2780.45556593415597396No Hit
GATACCACTGCTTCCCATGTACTCT2690.4408173967192698No Hit
AAGCAGTGGTATCAACGCAGAGTAC2680.43917867033741376No Hit
GCAGAGTACATGGGAAGCAGTGGTA2530.4145977746095734No Hit
GCAGTGGTATCAACGCAGAGTACAT2440.39984923717286924No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2360.3867394261180211No Hit
CCACTGCTTCCCATGTACTCTGCGT2360.3867394261180211No Hit
GTTGATACCACTGCTTCCCATGTAC2140.3506874457171886No Hit
ATCAACGCAGAGTACATGGGAAGCA1950.3195516444619242No Hit
CTTCCCATGTACTCTGCGTTGATAC1930.31627419169821214No Hit
ATGGGAAGCAGTGGTATCAACGCAG1880.308080559788932No Hit
ATCAACGCAGAGTACTTTTTTTTTT1850.303164380643364No Hit
CCATGTACTCTGCGTTGATACCACT1730.2834996640610917No Hit
CTTATACACATCTCCGAGCCCACGA1670.27366730576995557No Hit
GTGGTATCAACGCAGAGTACTTTTT1540.25236386280582734No Hit
TTCCCATGTACTCTGCGTTGATACC1500.2458089572784032No Hit
TGATACCACTGCTTCCCATGTACTC1380.22614424069613095No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1350.2212280615505629No Hit
AAAAAGTACTCTGCGTTGATACCAC1240.20320207135014665No Hit
GTACATGGTAAGCAGTGGTATCAAC1090.17862117562230634No Hit
GGAAGCAGTGGTATCAACGCAGAGT1080.1769824492404503No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1000.16387263818560216No Hit
TCAACGCAGAGTACATGGGAAGCAG990.16223391180374613No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA940.154040279894466No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT840.13765301607590583No Hit
GTACTGGTTCACTATCGGTCAGTCA770.12618193140291367No Hit
ATACACATCTCCGAGCCCACGAGAC760.12454320502105762No Hit
AAAGTACTCTGCGTTGATACCACTG740.1212657522573456No Hit
CCCCATGTACTCTGCGTTGATACCA680.11143339396620945No Hit
CTGCGTTGATACCACTGCTTCCCAT670.10979466758435344No Hit
CAACGCAGAGTACTTTTTTTTTTTT670.10979466758435344No Hit
TCAACGCAGAGTACTTTTTTTTTTT650.1065172148206414No Hit
ACCATGTACTCTGCGTTGATACCAC640.10487848843878539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC250.005888534719.0436233
AAAAGTA250.005888534719.0436232
ATACACA250.005888534719.0436234
AAGTACT307.490197E-419.0436214
AGTACTC307.490197E-419.0436215
CCATGTA8050.010.0540862
TGCGTTG8550.09.87906812
CTGCGTT8650.09.75685611
CTCTGCG8850.09.6435129
TCTGCGT8850.09.64351210
CGTTGAT8850.09.54418614
TACTCTG9100.09.4983597
TTGATAC8950.09.43754616
GTTGATA9000.09.38511515
TGATACC8900.09.38393117
ACTCTGC9200.09.284258
GATACCA9100.09.28198218
CCCATGT8600.09.2048071
GCGTTGA9250.09.13146413
GTACTCT9500.09.1208936
Produced by FastQC (version 0.11.5)