FastQCFastQC Report
Thu 26 May 2016
SRR1513205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882438
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50950.17676008989612266No Hit
GTCCTAAAGTGTGTATTTCTCATTT40730.14130399335562466No Hit
CTGTAGGACGTGGAATATGGCAAGA40330.13991627920531163No Hit
GTACATGGGAAGCAGTGGTATCAAC34680.12031481683214003No Hit
GTCCTACAGTGTGCATTTCTCATTT34270.11889240982806916No Hit
CTTTAGGACGTGAAATATGGCGAGG33820.117331231408967No Hit
CCCATGTACTCTGCGTTGATACCAC28940.10040111877514798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12200.013.3583344
TATACCG500.001486546513.3160145
GGCGAGG11750.011.952519
AGGACCT19100.011.8519515
TGTAGGA29550.011.6515532
GTAGGAC27250.011.6116843
GTCCTAC27550.011.511531
CGTGCGC752.0991503E-411.38579710
GGACCTG19550.011.305796
TGGCGAG27500.011.17975218
TCCTACA29700.011.1431282
CGTTAGG951.2805418E-511.0607771
CTGTAGG30000.011.0490461
AGGACGT22050.010.9564885
GGACGTG21000.010.8413576
TAGGACT6450.010.6387064
GAATCGA909.662086E-510.5402096
CCTACAG29800.010.4901383
ATGGCGA29950.010.45585517
TTAGGAC17500.010.4020493
Produced by FastQC (version 0.11.5)