##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513205_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2882438
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.557768805434844	31.0	30.0	34.0	25.0	34.0
2	29.384469327701066	31.0	30.0	34.0	23.0	34.0
3	29.592881789651678	31.0	30.0	34.0	24.0	34.0
4	32.67082414261816	37.0	33.0	37.0	23.0	37.0
5	32.8237405279836	37.0	33.0	37.0	25.0	37.0
6	33.18252153211969	37.0	35.0	37.0	27.0	37.0
7	33.12825115405778	37.0	35.0	37.0	27.0	37.0
8	33.016413189112825	37.0	35.0	37.0	26.0	37.0
9	34.27138346080644	39.0	35.0	39.0	25.0	39.0
10	33.8519503281597	39.0	34.0	39.0	21.0	39.0
11	34.31927972084742	39.0	35.0	39.0	27.0	39.0
12	34.310245007871806	39.0	35.0	39.0	26.0	39.0
13	34.357972660643526	39.0	35.0	39.0	27.0	39.0
14	34.59448668106651	40.0	34.0	41.0	17.0	41.0
15	35.03536450740658	40.0	34.0	41.0	24.0	41.0
16	34.88611585054041	40.0	34.0	41.0	20.0	41.0
17	34.50889490077497	39.0	34.0	41.0	17.0	41.0
18	34.24433691201684	39.0	33.0	41.0	17.0	41.0
19	34.86551245855071	40.0	34.0	41.0	22.0	41.0
20	35.08291834898097	40.0	35.0	41.0	24.0	41.0
21	35.1254281965475	40.0	36.0	41.0	24.0	41.0
22	34.81377292417044	40.0	35.0	41.0	18.0	41.0
23	34.36164940928478	40.0	34.0	41.0	16.0	41.0
24	34.6162772625118	40.0	34.0	41.0	18.0	41.0
25	34.26132669635913	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.901697944886628
1101	2	1.1339331763668739
1101	3	1.0092942320795864
1101	4	1.4219481172143773
1101	5	1.2928161969782934
1101	6	1.0843818769055105
1101	7	0.9766725615197842
1101	8	0.9991839619687681
1101	9	1.3570936257704744
1101	10	1.667956341575568
1101	11	1.2474174571632304
1101	12	1.121280461509258
1101	13	1.040136947054691
1101	14	2.0567777615734215
1101	15	1.5860712675473607
1101	16	1.6249607181249175
1101	17	2.051550334242634
1101	18	2.036302275403095
1101	19	1.6051708342826387
1101	20	1.2636068952934494
1101	21	1.28991756429933
1101	22	1.5969110678640774
1101	23	1.837316159439915
1101	24	1.3504462326057407
1101	25	1.7057273938605633
1102	1	0.8925724169763782
1102	2	1.081926627113834
1102	3	1.0112453356970867
1102	4	1.3204256736392068
1102	5	1.2926486553408338
1102	6	0.9149474111812239
1102	7	0.8997504414848905
1102	8	0.9914369263070526
1102	9	1.3241534829622381
1102	10	1.5572283535489078
1102	11	1.1605122089634676
1102	12	1.1298622915906549
1102	13	1.0848420429540724
1102	14	2.098500616326028
1102	15	1.638514825907606
1102	16	1.6239880240497868
1102	17	2.0240688548779957
1102	18	2.3475599527604842
1102	19	1.6460451662874078
1102	20	1.5129504850887656
1102	21	1.417116968545919
1102	22	1.5575933041358923
1102	23	1.876559790196076
1102	24	1.5957139097894242
1102	25	1.9159249641145877
1103	1	0.7182960685471755
1103	2	0.9934234105636044
1103	3	0.7045995045822764
1103	4	1.2058743850791913
1103	5	1.0783999115413465
1103	6	0.7810550510761871
1103	7	0.7764315460993387
1103	8	0.8213932327349553
1103	9	1.2136647968154861
1103	10	1.452062143794997
1103	11	1.0146656573546124
1103	12	0.9723191036356482
1103	13	0.9433678203455145
1103	14	1.8280194917345511
1103	15	1.4712753466643207
1103	16	1.49293240286719
1103	17	1.8051335978057494
1103	18	2.0079630761120697
1103	19	1.4730245643512845
1103	20	1.225714737093277
1103	21	1.197116985055871
1103	22	1.3831345208033383
1103	23	1.78305207881305
1103	24	1.4120896299326375
1103	25	1.6148012146398045
1104	1	0.8667082455606057
1104	2	1.026751553077549
1104	3	0.9116186278853249
1104	4	1.3319512773356266
1104	5	1.260949512253788
1104	6	0.983637321508148
1104	7	0.8945807594468818
1104	8	1.0553686055412257
1104	9	1.3158863053000474
1104	10	1.481184587113681
1104	11	1.03635273854065
1104	12	1.0159111182088765
1104	13	0.9753695462929599
1104	14	1.968699456631633
1104	15	1.5943551895771506
1104	16	1.6841386681861934
1104	17	2.0665022222223897
1104	18	2.166724773704665
1104	19	1.548920345675043
1104	20	1.2280522279485169
1104	21	1.260091194794171
1104	22	1.501960325015368
1104	23	1.7496819231046885
1104	24	1.6072370248712602
1104	25	1.883140785067063
1105	1	0.5993931579409484
1105	2	0.869557985224084
1105	3	0.7897888502179846
1105	4	1.0947155857852167
1105	5	1.0569288290452903
1105	6	0.746194561222751
1105	7	0.7393407372017791
1105	8	0.8346510827932434
1105	9	0.9901695516645077
1105	10	1.2576248980717608
1105	11	0.8072814713983192
1105	12	0.7970359155773323
1105	13	0.7398649493670462
1105	14	1.6682320729155151
1105	15	1.2393518173148905
1105	16	1.422752845886663
1105	17	1.7045200419660986
1105	18	1.3656551157822179
1105	19	1.2103546812210482
1105	20	1.060326552608629
1105	21	1.054538202946759
1105	22	1.2510829452039758
1105	23	1.458109028993725
1105	24	1.2544084659513288
1105	25	1.5580180228884615
1106	1	0.4272182548143313
1106	2	0.6179909669300301
1106	3	0.5450081864005121
1106	4	0.892170789816447
1106	5	0.8590647973037449
1106	6	0.5619484576082314
1106	7	0.5223513138505993
1106	8	0.5005080557058008
1106	9	0.6442753367041547
1106	10	0.5876680533961434
1106	11	0.5270814403246291
1106	12	0.5578914159680721
1106	13	0.35605619632634244
1106	14	1.3913921742992272
1106	15	0.9371671514845872
1106	16	1.0423633580691742
1106	17	1.3100697100109855
1106	18	1.4564297086357314
1106	19	0.8756249643394582
1106	20	0.6718973275725091
1106	21	0.5456706070067483
1106	22	0.8284552706057866
1106	23	1.358098130013353
1106	24	0.9267167076675236
1106	25	1.2385167324028075
1107	1	0.5424381419911377
1107	2	0.7681416916014463
1107	3	0.7242112369747495
1107	4	1.062010496248348
1107	5	0.8662729573861512
1107	6	0.6813472548868731
1107	7	0.5777540617284629
1107	8	0.7493025908764821
1107	9	0.9171065288132638
1107	10	1.2118166938604418
1107	11	0.8177746075421339
1107	12	0.6915245705739252
1107	13	0.631835373459424
1107	14	1.5446780428013014
1107	15	1.0025259996587863
1107	16	1.1256573405965824
1107	17	1.5431955925463399
1107	18	1.7218647505006928
1107	19	1.1682518634141985
1107	20	1.0140464884650697
1107	21	0.951888145189848
1107	22	1.079375372812578
1107	23	1.3645810367299163
1107	24	1.1473414003259563
1107	25	1.4076429283590102
1108	1	0.5205020161523919
1108	2	0.7325415298200149
1108	3	0.6206033532852722
1108	4	1.0203297006836536
1108	5	0.8848258310861965
1108	6	0.5391089833747671
1108	7	0.453093641895812
1108	8	0.4910400796049501
1108	9	0.7643565620567259
1108	10	0.9811945073893114
1108	11	0.6830384479374629
1108	12	0.39398232269304856
1108	13	0.3810817354773377
1108	14	1.4787500605349706
1108	15	0.956444620074592
1108	16	1.019071580353959
1108	17	1.3549638394162784
1108	18	1.604027896911866
1108	19	0.9298599269974162
1108	20	0.8006536863864966
1108	21	0.7158041450578949
1108	22	0.9697875589538327
1108	23	1.3498220666809928
1108	24	1.018595005628029
1108	25	1.2007269668054619
1109	1	0.3403356244143865
1109	2	0.5467639353787348
1109	3	0.46933264923980644
1109	4	0.7417807660117006
1109	5	0.7738843434359737
1109	6	0.3056157485778144
1109	7	0.4004342061428616
1109	8	0.30093542139414353
1109	9	0.5479113051556368
1109	10	0.8403551304698524
1109	11	0.5659179769367029
1109	12	0.43915261351614987
1109	13	0.4332487700203487
1109	14	1.3332900661422684
1109	15	0.8255850545533505
1109	16	0.955529251020792
1109	17	1.3267789808321098
1109	18	1.494513850288513
1109	19	0.8836179901496592
1109	20	0.7364307608821292
1109	21	0.6785918125964301
1109	22	0.8971861024668684
1109	23	1.1575703626669238
1109	24	0.8851011671868534
1109	25	1.150962030428289
1110	1	0.5474925243086339
1110	2	0.7558877782307789
1110	3	0.7255192569795099
1110	4	1.1032793749112102
1110	5	0.8670522109275325
1110	6	0.6583013238740989
1110	7	0.6396170910573886
1110	8	0.6443728361878769
1110	9	0.8042328062668176
1110	10	1.077548035703579
1110	11	0.764839475194762
1110	12	0.583699732965492
1110	13	0.40391178051878995
1110	14	1.2531418286103673
1110	15	1.0142648391131104
1110	16	1.2070110743658233
1110	17	1.5186640456305796
1110	18	1.1081361854450122
1110	19	0.8244115956796065
1110	20	0.6174355007508936
1110	21	0.5458774529903607
1110	22	0.8679504846282597
1110	23	-0.4587090593744563
1110	24	0.36858562629593905
1110	25	0.8632223205972451
1111	1	0.2991879112849496
1111	2	0.6100170021674671
1111	3	0.5339274360983879
1111	4	0.7822965880532777
1111	5	0.7384736282087516
1111	6	0.555490778335006
1111	7	0.48518824593202226
1111	8	0.4844016735370573
1111	9	0.6493117008265088
1111	10	0.9068600198369907
1111	11	0.5193351816294793
1111	12	0.49845091883378245
1111	13	0.296481545995384
1111	14	1.183147331451849
1111	15	0.848940010784311
1111	16	0.8174506133332429
1111	17	1.188296474069837
1111	18	1.5170855831080274
1111	19	0.9652128740686337
1111	20	0.6242435809217852
1111	21	0.38215760356270323
1111	22	0.6991457636901686
1111	23	1.1240660053345124
1111	24	0.8165914116192639
1111	25	1.0199163764969086
1112	1	0.06315067724239753
1112	2	0.34184324330221827
1112	3	0.18375013113384853
1112	4	0.6411175100390949
1112	5	0.42486547218038595
1112	6	0.24010957266124677
1112	7	0.07760845869798061
1112	8	0.34078465226046006
1112	9	0.5316255306137379
1112	10	0.7348742042341527
1112	11	0.17868801850890748
1112	12	0.21697928762811358
1112	13	0.2053911204634673
1112	14	0.687136693153974
1112	15	0.48834603915310737
1112	16	0.45552685446799757
1112	17	0.8423301620130985
1112	18	0.9439472852356872
1112	19	0.4195568456998373
1112	20	0.3762382617728477
1112	21	0.24563278086767326
1112	22	0.47950923886747177
1112	23	0.8665305443416074
1112	24	0.5258112697752964
1112	25	0.8157940360713738
1113	1	0.241808921236629
1113	2	0.3658681346592765
1113	3	0.31083029620520364
1113	4	0.6451737191263334
1113	5	0.6023078588566548
1113	6	0.27701168337111426
1113	7	0.286343072519216
1113	8	0.29655203670031227
1113	9	0.4174256746862852
1113	10	0.7500048131368331
1113	11	0.3586332831048864
1113	12	0.2897721174294716
1113	13	0.18121247521621342
1113	14	0.8622817465847987
1113	15	0.44317594173934793
1113	16	0.5954184704817038
1113	17	0.8932225034901435
1113	18	0.7810983252103227
1113	19	0.5856356183053393
1113	20	0.3940011274667867
1113	21	0.3496249479039335
1113	22	0.5074810195601813
1113	23	0.5951876897434474
1113	24	0.414166327773124
1113	25	0.8093473586071127
1114	1	-0.9925276850665732
1114	2	-0.6648735864247932
1114	3	-0.7263834092675943
1114	4	-0.5328201367499616
1114	5	-3.422453933853774
1114	6	-1.4107687296581908
1114	7	-1.1244378444182317
1114	8	-0.7936392339956839
1114	9	-0.4932446464827649
1114	10	-0.336433283802684
1114	11	-0.7628072784508504
1114	12	-0.8093335913598025
1114	13	-0.8726259591813204
1114	14	-6.264344747150652
1114	15	-2.594762333439789
1114	16	-1.496300857452546
1114	17	-0.5454157313676689
1114	18	-6.446680392868366
1114	19	-2.6034810739373526
1114	20	-1.5880893922427433
1114	21	-1.2343153131329103
1114	22	-1.0547894568813803
1114	23	-0.5979672054719387
1114	24	-0.6328277438022596
1114	25	-0.45398716427512653
1115	1	-0.758130532905934
1115	2	-0.46121694530104307
1115	3	-0.4309144436698311
1115	4	-3.252192024157832
1115	5	-1.1873772398584528
1115	6	-0.6722883511367712
1115	7	-0.6443675687758983
1115	8	-0.5648361108780051
1115	9	-0.22666809088210726
1115	10	-0.7211817111516723
1115	11	-0.6742801616821552
1115	12	-0.6725191686441221
1115	13	-0.750233977417821
1115	14	-6.229679871716151
1115	15	-2.4807707360219666
1115	16	-1.3438914510111175
1115	17	-0.4438424278698889
1115	18	-0.2685571893375496
1115	19	-0.6133431531815177
1115	20	-0.6339553753840121
1115	21	-0.7338004025674607
1115	22	-1.8210379175412328
1115	23	-1.1268058372947465
1115	24	-0.8358894595801445
1115	25	-0.41308402062146854
1116	1	-0.40887839002603243
1116	2	-0.14580477947878379
1116	3	-0.2966952804285832
1116	4	-0.10212797674362406
1116	5	-0.6583201070158324
1116	6	-0.41477759690677374
1116	7	-0.30444021957409717
1116	8	-0.23207506190244231
1116	9	-0.2840826146688755
1116	10	-0.14823266230356325
1116	11	-0.3628236406357317
1116	12	-0.7202660379682797
1116	13	-0.6410251701017557
1116	14	0.12155104948322304
1116	15	-0.3049921297701701
1116	16	-0.38893048488296245
1116	17	0.016914032634204546
1116	18	-0.10631329933340794
1116	19	-0.25090871415402205
1116	20	-0.8183813384850538
1116	21	-0.8087976940947215
1116	22	-0.8555221924671486
1116	23	-1.4551976417748804
1116	24	-1.1863760287417904
1116	25	-0.5621583602320825
1201	1	0.890598872692081
1201	2	0.9797617743787477
1201	3	0.9716665849022732
1201	4	1.359247211369727
1201	5	1.1206234436693805
1201	6	0.8997497704953901
1201	7	0.8078621465916385
1201	8	0.8665430144074051
1201	9	1.0355531931646524
1201	10	1.5668472108038216
1201	11	0.975360532642874
1201	12	1.011802325781126
1201	13	0.8612121718418138
1201	14	1.7746312570143559
1201	15	1.4847014485874297
1201	16	1.5705067882917803
1201	17	1.8386298148442748
1201	18	1.3309813067884306
1201	19	1.2937291779401576
1201	20	1.2104221545944966
1201	21	1.2016746676669854
1201	22	1.5092798027708412
1201	23	1.8126796388419706
1201	24	1.4632172224928652
1201	25	1.7703473920918071
1202	1	0.8282482113181331
1202	2	1.0498948766818081
1202	3	0.9476318349287212
1202	4	1.3670882230510486
1202	5	1.1526450411237832
1202	6	0.8718354336057743
1202	7	0.8438479630123084
1202	8	0.9854850052358657
1202	9	1.1884576764655606
1202	10	1.5164593837620686
1202	11	1.1131407043583437
1202	12	0.9844540832717783
1202	13	1.0715732734254715
1202	14	1.9480880023257754
1202	15	1.4772636616075374
1202	16	1.5152033168355388
1202	17	1.9586186100981706
1202	18	2.062480189833437
1202	19	1.5570399590262767
1202	20	1.2965366359120623
1202	21	1.2174260087088413
1202	22	1.4034886115862903
1202	23	1.7426151019332323
1202	24	1.4084229404383706
1202	25	1.7537958348647678
1203	1	0.6986669261494214
1203	2	0.8419397894986638
1203	3	0.807940114617768
1203	4	1.1523842690014945
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1203	6	0.690813934849146
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2211	13	-0.64164222018141
2211	14	-0.044365098174530715
2211	15	-0.4010348557799972
2211	16	-6.5686012437991685
2211	17	-2.0098875209583156
2211	18	-0.6824738963099719
2211	19	-0.7284402479610534
2211	20	-0.8819415291169719
2211	21	-0.8163417018405283
2211	22	-0.7392727491415982
2211	23	-0.2915656207530759
2211	24	-0.6554190804477074
2211	25	-7.432638551484374
2212	1	-1.3643585430690308
2212	2	-3.75875739898445
2212	3	-1.6660247059148041
2212	4	-3.359184954703885
2212	5	-1.0671635478149604
2212	6	-0.9371855709807022
2212	7	-0.885522193983725
2212	8	-0.7979172933347911
2212	9	-0.867990547856742
2212	10	-0.3156678148516292
2212	11	-0.7673092293735522
2212	12	-0.8254747505736688
2212	13	-1.0529910151736956
2212	14	-6.314036526317388
2212	15	-4.955883919417818
2212	16	-2.7367557335347925
2212	17	-7.066160513422872
2212	18	-2.7733036114877585
2212	19	-1.6922328201479147
2212	20	-1.8007898290145476
2212	21	-1.384649350841137
2212	22	-1.3316291504147557
2212	23	-0.7585562383929556
2212	24	-1.0277913752189178
2212	25	-0.8837044560431977
2213	1	-0.2549584260010711
2213	2	-0.12476379423788231
2213	3	-0.24389682124625267
2213	4	0.20992120045464446
2213	5	-0.06008873830987227
2213	6	-0.4362221134709827
2213	7	-0.3610148698992717
2213	8	-0.39737450742868674
2213	9	-0.24971745954773183
2213	10	-3.9932170717062867
2213	11	-1.5927716719781344
2213	12	-1.015615076442053
2213	13	-0.8377127515016412
2213	14	-0.0394450518068723
2213	15	-0.6971377777663008
2213	16	-0.3533903714428064
2213	17	-0.03876353260236698
2213	18	0.012957527371270317
2213	19	-0.48236031542502644
2213	20	-0.843879410341799
2213	21	-0.6311551620968743
2213	22	-1.0288270172797453
2213	23	-1.0802382197511946
2213	24	-0.7283698434109525
2213	25	-0.5058035479343914
2214	1	-0.9707244366996655
2214	2	-0.7120395408859572
2214	3	-0.7274859457020071
2214	4	-0.29245190568824597
2214	5	-3.678661451495106
2214	6	-1.4661255592892175
2214	7	-1.1828728214549784
2214	8	-0.8158716484576516
2214	9	-0.7817907303080247
2214	10	-4.333122944435228
2214	11	-1.8468640446761313
2214	12	-1.2477895489400694
2214	13	-1.001561568569187
2214	14	-0.3390410786951108
2214	15	-0.5552951600732001
2214	16	-0.6770461240330832
2214	17	-0.3749494295740874
2214	18	0.001906833024655441
2214	19	-0.8214276970867971
2214	20	-1.0259466467400173
2214	21	-1.0135765168045765
2214	22	-0.6402870827492819
2214	23	-0.5018573422276802
2214	24	-0.955362444624761
2214	25	-0.639831644801923
2215	1	-2.238097280501467
2215	2	-4.741522162735432
2215	3	-2.959514043980331
2215	4	-1.9782155540877469
2215	5	-1.836144423478892
2215	6	-2.123389369078751
2215	7	-2.257246819605829
2215	8	-2.4454930110271995
2215	9	-8.260840772160016
2215	10	-3.9264366921306326
2215	11	-3.1707986789772598
2215	12	-3.427237321484064
2215	13	-2.9483634746128367
2215	14	-7.67677144245183
2215	15	-4.780330857360209
2215	16	-9.043597574628585
2215	17	-4.450732061818172
2215	18	-2.962425203717565
2215	19	-3.5175224959510487
2215	20	-3.178343261378913
2215	21	-3.3698532760927087
2215	22	-9.373583433146194
2215	23	-10.60144757566879
2215	24	-6.649317485191101
2215	25	-5.066834440405547
2216	1	-1.808499769382511
2216	2	-1.9000719545406604
2216	3	-1.6224294432488406
2216	4	-6.816970441122514
2216	5	-3.3641867201672113
2216	6	-2.643085844950118
2216	7	-2.0825939422311315
2216	8	-7.938217278773138
2216	9	-3.7540190652675776
2216	10	-2.0793303945933523
2216	11	-2.134883310178843
2216	12	-2.3157225902460254
2216	13	-2.0010826990334607
2216	14	-1.390833725418105
2216	15	-1.884614847008784
2216	16	-1.6651643466797381
2216	17	-1.4221762075156406
2216	18	-7.188064587044963
2216	19	-4.542021163147265
2216	20	-3.8356721271665677
2216	21	-3.095899172454473
2216	22	-2.707655445527557
2216	23	-2.097129543574333
2216	24	-2.408667820834907
2216	25	-1.8804137619311945
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	126114.0
3	2.0
4	1077.0
5	6280.0
6	7662.0
7	6859.0
8	6198.0
9	5932.0
10	5664.0
11	5290.0
12	5169.0
13	5161.0
14	5369.0
15	5408.0
16	5542.0
17	5865.0
18	6525.0
19	8050.0
20	10360.0
21	13357.0
22	16551.0
23	19754.0
24	23113.0
25	26255.0
26	29584.0
27	33461.0
28	37677.0
29	44514.0
30	53834.0
31	64309.0
32	82542.0
33	107330.0
34	142095.0
35	191832.0
36	272567.0
37	398054.0
38	1097082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38126618772039	18.425392006511057	12.494444091136714	25.69889771463184
2	14.158259628267682	19.761409850252456	42.21168067028131	23.86864985119855
3	18.734592947722373	24.220729687735336	29.536217073214	27.50846029132829
4	11.225811398616997	16.49809000669195	39.22247936649565	33.0536192281954
5	12.526364850575991	38.09418303745339	36.48185764371974	12.897594468250876
6	32.24059851910866	37.171177617089754	17.98399188316927	12.60423198063231
7	28.394025889846358	31.26997378814408	22.120356625589626	18.215643696419935
8	24.839006631271662	37.36756801208625	20.522451630857013	17.270973725785066
9	26.330858114438392	15.053436968666507	20.21496235122662	38.40074256566848
10	13.731706961608475	28.922068377290294	35.82808725277029	21.518137408330944
11	34.55986630870378	22.261398269105783	24.71581751110306	18.462917911087377
12	23.410213058934414	24.484738572842446	31.929933052104982	20.175115316118163
13	29.0576668415661	21.73729272335074	25.665971233207575	23.539069201875588
14	20.066862474563944	21.94361315540355	28.645129186018426	29.344395184014083
15	22.912667454969064	31.018044998485056	23.21492716648093	22.85436038006495
16	21.879050148186625	28.05101187123916	28.246893736950607	21.823044243623603
17	21.342175335132556	28.545928708670687	28.545581636846258	21.566314319350504
18	21.30027117613539	27.36786323338847	30.307802612647066	21.02406297782908
19	23.401842408998696	27.011946578051138	27.57644720303769	22.009763809912474
20	23.680314658959826	27.31167033824311	27.911372725392457	21.096642277404605
21	22.926535206066728	26.849044273641375	27.015836977657898	23.208583542634
22	23.01620825028018	28.123651814339016	27.203406096168003	21.656733839212798
23	21.883124815207086	27.81898553722163	28.08272753646927	22.21516211110201
24	22.985778453765658	27.020521330583875	28.167984530645242	21.82571568500523
25	22.54838537266958	27.2529227659573	28.040436657034597	22.158255204338527
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	177.0
1	177.0
2	333.5
3	490.0
4	490.0
5	490.0
6	1001.5
7	1513.0
8	1513.0
9	1513.0
10	2587.0
11	3661.0
12	3661.0
13	3661.0
14	7258.5
15	10856.0
16	10856.0
17	10856.0
18	20887.5
19	30919.0
20	30919.0
21	30919.0
22	50970.0
23	71021.0
24	71021.0
25	71021.0
26	102273.5
27	133526.0
28	133526.0
29	133526.0
30	173845.0
31	214164.0
32	214164.0
33	214164.0
34	248434.5
35	282705.0
36	282705.0
37	282705.0
38	308330.0
39	333955.0
40	333955.0
41	333955.0
42	351224.0
43	368493.0
44	368493.0
45	368493.0
46	378532.0
47	388571.0
48	388571.0
49	388571.0
50	370176.5
51	351782.0
52	351782.0
53	351782.0
54	311380.0
55	270978.0
56	270978.0
57	270978.0
58	230748.0
59	190518.0
60	190518.0
61	190518.0
62	154054.5
63	117591.0
64	117591.0
65	117591.0
66	90550.5
67	63510.0
68	63510.0
69	63510.0
70	46443.5
71	29377.0
72	29377.0
73	29377.0
74	20621.5
75	11866.0
76	11866.0
77	11866.0
78	8193.0
79	4520.0
80	4520.0
81	4520.0
82	3021.0
83	1522.0
84	1522.0
85	1522.0
86	1019.5
87	517.0
88	517.0
89	517.0
90	337.5
91	158.0
92	158.0
93	158.0
94	96.0
95	34.0
96	34.0
97	34.0
98	24.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16898889065436967
2	0.25901684615592774
3	0.2614800387727334
4	0.4620394263467245
5	0.25665773210039555
6	0.018213748222858567
7	0.01859536961419465
8	0.016895419780061184
9	0.018664755321710302
10	0.020954483669726808
11	0.01994839091074986
12	0.027546125883713718
13	0.027129811638619803
14	0.024354383337993737
15	0.03958454613767929
16	0.02078101940093768
17	0.04128449597181275
18	0.03177865404216847
19	0.026921654516072854
20	0.029211382864089357
21	0.035282632271708886
22	0.04340076005104013
23	0.02893384003402675
24	0.04811898816210444
25	0.03788459630354582
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2882438.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.9135098798529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.04378863997769	58.180130163016216
2	12.113916803388912	17.180807178416714
3	3.11314759003716	6.622926671506213
4	1.1704539078542975	3.3200397903415264
5	0.5294221652142237	1.8771591971765982
6	0.28215445200396916	1.2005137511896768
7	0.1615912899351945	0.8021303874722314
8	0.10838471195355116	0.614875227355453
9	0.08680244311863716	0.5539919316919961
>10	0.355326852627329	4.345454972873838
>50	0.02002925375239359	0.9802575662967645
>100	0.012006487365419719	1.6864754507100805
>500	0.0019340118014546373	0.9647452045698751
>1k	9.918009236802308E-4	1.491321521863601
>5k	4.959004618401154E-5	0.17917098551912486
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	5095	0.17676008989612266	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4073	0.14130399335562466	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4033	0.13991627920531163	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3468	0.12031481683214003	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3427	0.11889240982806916	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3382	0.117331231408967	No Hit
CCCATGTACTCTGCGTTGATACCAC	2894	0.10040111877514798	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.938570751565168E-5
2	0.0	0.0	0.0	0.0	6.938570751565168E-5
3	0.0	0.0	0.0	0.0	6.938570751565168E-5
4	0.0	0.0	0.0	3.469285375782584E-5	6.938570751565168E-5
5	0.0	3.469285375782584E-5	0.0	3.469285375782584E-5	1.0407856127347753E-4
6	0.0	3.469285375782584E-5	0.0	3.469285375782584E-5	1.0407856127347753E-4
7	0.0	3.469285375782584E-5	0.0	3.469285375782584E-5	1.3877141503130337E-4
8	0.0	3.469285375782584E-5	0.0	3.469285375782584E-5	1.3877141503130337E-4
9	0.0	3.469285375782584E-5	0.0	3.469285375782584E-5	1.3877141503130337E-4
10	0.0	3.469285375782584E-5	0.0	1.0407856127347753E-4	1.3877141503130337E-4
11	0.0	3.469285375782584E-5	0.0	1.0407856127347753E-4	1.3877141503130337E-4
12	0.0	3.469285375782584E-5	0.0	1.0407856127347753E-4	3.122356838204326E-4
13	0.0	3.469285375782584E-5	0.0	1.0407856127347753E-4	3.122356838204326E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1220	0.0	13.358334	4
TATACCG	50	0.0014865465	13.316014	5
GGCGAGG	1175	0.0	11.9525	19
AGGACCT	1910	0.0	11.851951	5
TGTAGGA	2955	0.0	11.651553	2
GTAGGAC	2725	0.0	11.611684	3
GTCCTAC	2755	0.0	11.51153	1
CGTGCGC	75	2.0991503E-4	11.385797	10
GGACCTG	1955	0.0	11.30579	6
TGGCGAG	2750	0.0	11.179752	18
TCCTACA	2970	0.0	11.143128	2
CGTTAGG	95	1.2805418E-5	11.060777	1
CTGTAGG	3000	0.0	11.049046	1
AGGACGT	2205	0.0	10.956488	5
GGACGTG	2100	0.0	10.841357	6
TAGGACT	645	0.0	10.638706	4
GAATCGA	90	9.662086E-5	10.540209	6
CCTACAG	2980	0.0	10.490138	3
ATGGCGA	2995	0.0	10.455855	17
TTAGGAC	1750	0.0	10.402049	3
>>END_MODULE