FastQCFastQC Report
Thu 26 May 2016
SRR1513205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882438
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50580.17547645430708309No Hit
CTGTAGGACGTGGAATATGGCAAGA43430.15067106387023763No Hit
CTTTAGGACGTGAAATATGGCGAGG41420.14369780026491463No Hit
GTCCTAAAGTGTGTATTTCTCATTT41130.14269170750593768No Hit
GTACATGGGAAGCAGTGGTATCAAC39120.1357184439006147No Hit
CCCATGTACTCTGCGTTGATACCAC37560.13030635871439386No Hit
GTCCTACAGTGTGCATTTCTCATTT37470.12999412303057342No Hit
GTATCAACGCAGAGTACTTTTTTTT34990.12139029529863261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT855.4569682E-1116.7985346
TAGGACC11200.013.6847594
GTCCGCT550.003093195412.0785211
CTACACG752.0351792E-411.42379954
AGGACCT20600.011.41455655
TAGACGT600.00578718111.1064714
TATACCG600.00578718111.1064715
GTCCTAC28550.010.9083721
GGCGAGG11150.010.80766619
TGGCGAG27000.010.61577618
TGGACCG909.3214294E-510.5775915
GGACCTG19900.010.52370556
GTAGGAC27600.010.5204713
AAATGTC8300.010.4062557
AATGTCC8300.010.2899368
CTAGGAC4450.010.2689413
TCCTACA31800.010.209442
GAAATGT9000.010.1537816
GGCGAGA17200.010.15061719
TGTAGGA30750.010.0936242
Produced by FastQC (version 0.11.5)