FastQCFastQC Report
Thu 26 May 2016
SRR1513204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590458
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC29200.18359491416937762No Hit
CCCATGTACTCTGCGTTGATACCAC26120.16422942322274464No Hit
GTATCAACGCAGAGTACTTTTTTTT19360.12172594309312161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT500.001510862113.2867698
CTAAGAC1354.5965862E-911.2792743
CGCGGTT600.005906984711.07544318
GTCCTAA2800.010.89981651
TATACTG2800.010.8689015
GTAGACC2650.010.7738353
GTAGGAC5050.010.7418333
TGCACCG803.733238E-410.6990765
GTATTAA2150.010.6463321
AGGACCG1451.4186298E-810.4941125
TAGGACC2100.010.42264
GTCTAGC1105.7678426E-610.404371
TGTAGGA6600.010.3879562
GTCTTAC1303.638761E-710.2709811
ATTATAC1303.7476093E-710.2489563
GGACGTG4950.010.1606416
GCTAGAC1502.3770554E-810.1513463
TTTATAC2450.010.0995543
TAGAACA3700.010.0306824
TAACACT1909.640644E-1110.0171444
Produced by FastQC (version 0.11.5)