##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513204_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1590458
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.793522997778	31.0	30.0	34.0	20.0	34.0
2	28.648617568021287	31.0	29.0	34.0	16.0	34.0
3	28.869053442467514	31.0	30.0	34.0	16.0	34.0
4	31.960809402071604	35.0	32.0	37.0	17.0	37.0
5	32.048865169655535	35.0	33.0	37.0	19.0	37.0
6	32.372420397143465	36.0	33.0	37.0	22.0	37.0
7	32.296504528884135	36.0	33.0	37.0	22.0	37.0
8	32.243949855953446	36.0	33.0	37.0	21.0	37.0
9	33.348437997105236	38.0	33.0	39.0	17.0	39.0
10	32.92918769310475	38.0	33.0	39.0	16.0	39.0
11	33.33766059839367	38.0	33.0	39.0	17.0	39.0
12	33.33272302695198	38.0	33.0	39.0	17.0	39.0
13	33.37041217058231	38.0	34.0	39.0	17.0	39.0
14	33.59935188480299	39.0	33.0	41.0	10.0	41.0
15	33.957849248455474	39.0	33.0	41.0	16.0	41.0
16	33.74743627307354	39.0	33.0	41.0	10.0	41.0
17	33.39261709520151	39.0	32.0	41.0	10.0	41.0
18	33.10315456302524	38.0	32.0	41.0	10.0	41.0
19	33.708732327417636	39.0	33.0	41.0	10.0	41.0
20	33.919470366397604	39.0	34.0	41.0	10.0	41.0
21	33.95570709820693	39.0	34.0	41.0	10.0	41.0
22	33.65905858564011	39.0	33.0	41.0	2.0	41.0
23	33.15746910638319	39.0	33.0	41.0	2.0	41.0
24	33.36037481027478	39.0	33.0	41.0	2.0	41.0
25	32.94660971870996	39.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5813528639378163
1101	2	1.789854683073294
1101	3	1.6136729104035936
1101	4	2.1469566940236753
1101	5	2.0578790549620187
1101	6	1.8158821506659635
1101	7	1.7000184830188445
1101	8	1.7370408795289443
1101	9	2.265472869369262
1101	10	2.5927940518308503
1101	11	2.0839592699570417
1101	12	1.9592969312102468
1101	13	1.9214306013773168
1101	14	2.9791790727999654
1101	15	2.54599777328783
1101	16	2.686116394921001
1101	17	2.8813325857954553
1101	18	3.0218978738705644
1101	19	2.6071362471855366
1101	20	2.1643328009974354
1101	21	2.2703367018476754
1101	22	2.508494425139723
1101	23	2.779998928474569
1101	24	2.5195640743179553
1101	25	2.796328460920151
1102	1	1.2868536753225008
1102	2	1.3904171724447778
1102	3	1.2627511467411061
1102	4	1.5930252927414763
1102	5	1.563794706197978
1102	6	1.1827155641563962
1102	7	1.205078838664221
1102	8	1.3131169867964765
1102	9	1.7073558205201351
1102	10	2.002327775333697
1102	11	1.6166091404521055
1102	12	1.516151810184489
1102	13	1.5043063802228005
1102	14	2.494484351005269
1102	15	2.0586059334381304
1102	16	2.2288520199021065
1102	17	2.5687345115428997
1102	18	2.821190657596972
1102	19	2.1445243406399754
1102	20	1.9361221724955726
1102	21	1.8485544864659076
1102	22	1.9995017154959527
1102	23	2.298432267638816
1102	24	2.130940107388234
1102	25	2.5090053025957673
1103	1	1.2010523616691238
1103	2	1.5163572481008742
1103	3	1.2944486245635325
1103	4	1.7886632479966202
1103	5	1.6539184548164307
1103	6	1.3090000603349097
1103	7	1.28170317681532
1103	8	1.3935024084099332
1103	9	1.8193775306546343
1103	10	2.1468743675794073
1103	11	1.6522332393828592
1103	12	1.4610614210929356
1103	13	1.4691598701780322
1103	14	2.529299217875945
1103	15	2.2064461001683284
1103	16	2.115307675759844
1103	17	2.553550620655777
1103	18	2.8203668025716055
1103	19	2.454466750291701
1103	20	2.052457657392395
1103	21	2.0797919797489257
1103	22	2.2225744832044754
1103	23	2.571022512985195
1103	24	2.3247606978011106
1103	25	2.4953108246107902
1104	1	1.17893313129656
1104	2	1.3350574033656812
1104	3	1.13710194326044
1104	4	1.6921333245553107
1104	5	1.56653686365593
1104	6	1.343013648477708
1104	7	1.2712245684626708
1104	8	1.295206789307418
1104	9	1.7537604201623225
1104	10	1.8954308569842482
1104	11	1.5700160749222505
1104	12	1.4481764201816887
1104	13	1.4350862387758312
1104	14	2.4456537302328485
1104	15	2.010350748646836
1104	16	2.0647516751777815
1104	17	2.694940064061825
1104	18	2.9218216860664867
1104	19	2.1954737534549267
1104	20	1.8350942769992713
1104	21	1.8452090402287382
1104	22	2.0494560623563203
1104	23	2.433925950709032
1104	24	2.2195968362142438
1104	25	2.5917763251667196
1105	1	0.9646574195114681
1105	2	1.1213914154401294
1105	3	1.108772003632673
1105	4	1.5690775606143212
1105	5	1.3828805209988353
1105	6	1.0905270982687227
1105	7	0.9951258294922098
1105	8	1.0548594167499878
1105	9	1.3858903097775794
1105	10	1.7905956481820482
1105	11	1.1936957091165112
1105	12	1.1110888625780007
1105	13	1.039314295790085
1105	14	1.9611248837355078
1105	15	1.7000463153956673
1105	16	1.8838780281946086
1105	17	2.129643168409345
1105	18	1.6073326352323534
1105	19	1.6014541056858391
1105	20	1.3764307539756615
1105	21	1.4088982482450305
1105	22	1.6141246580766833
1105	23	1.795887726867356
1105	24	1.7552024519038767
1105	25	2.0899667299107634
1106	1	0.9034271724014893
1106	2	1.246617420318696
1106	3	1.1488738171866046
1106	4	1.5826633067167712
1106	5	1.4918609006785495
1106	6	0.9697791928704831
1106	7	0.9832789740462431
1106	8	1.0652244607631438
1106	9	1.397616261628258
1106	10	1.0512548909437385
1106	11	1.146279015935903
1106	12	1.2589386875859603
1106	13	1.0028451049957283
1106	14	2.2895423193066193
1106	15	1.7811637191500367
1106	16	1.8944293061668205
1106	17	2.1511690990488574
1106	18	2.334056147799963
1106	19	1.762338186712455
1106	20	1.490632047856245
1106	21	1.4258294180403368
1106	22	1.6742578641430725
1106	23	2.134885711807435
1106	24	1.7347523511508811
1106	25	2.0263192593412427
1107	1	1.0536395402408019
1107	2	1.2353471598196641
1107	3	1.1300005396567485
1107	4	1.467838403241334
1107	5	1.5335542685647745
1107	6	1.2056816388953848
1107	7	1.0224007454065287
1107	8	1.3110095318146016
1107	9	1.4845775110521444
1107	10	1.8871854870915854
1107	11	1.3213551178614225
1107	12	1.1974149379895778
1107	13	1.1666451508086695
1107	14	2.0844543225742456
1107	15	1.4431930670331212
1107	16	1.7017091487469145
1107	17	2.108401886766572
1107	18	2.317114189489743
1107	19	1.8150062388067454
1107	20	1.5595824622426093
1107	21	1.5077766337999918
1107	22	1.666519064166252
1107	23	1.9556632623603676
1107	24	1.7552831741556076
1107	25	2.1853478593141915
1108	1	0.6990596484881664
1108	2	0.8828584873752838
1108	3	0.6960161778981266
1108	4	1.2298425048899055
1108	5	1.1050466810798873
1108	6	0.7794115967489645
1108	7	0.6849059713361854
1108	8	0.799840166496697
1108	9	0.9309936532426306
1108	10	1.322199498232706
1108	11	0.9623117172218869
1108	12	0.6716542121220783
1108	13	0.6109921073402376
1108	14	1.6139556841407767
1108	15	1.1036613103298123
1108	16	1.2643126786505334
1108	17	1.6480616081256585
1108	18	1.874179646377236
1108	19	1.1857783204781356
1108	20	0.8999738137527657
1108	21	0.9986976403422929
1108	22	1.2551206053917738
1108	23	1.5844753661784026
1108	24	1.2549015400497723
1108	25	1.5579292425753977
1109	1	0.4912516028114453
1109	2	0.7356555650227179
1109	3	0.5679609832760271
1109	4	0.8287266834213405
1109	5	0.9558161694725378
1109	6	0.4690798109857681
1109	7	0.46194150176642523
1109	8	0.4954192335581986
1109	9	0.7200381974824595
1109	10	1.10280970851273
1109	11	0.6925304217974997
1109	12	0.6534444638113044
1109	13	0.5303888887472965
1109	14	1.5014510949469937
1109	15	1.1032504408171349
1109	16	1.0674591392456136
1109	17	1.5433196618010925
1109	18	1.7129452999877657
1109	19	1.0769807640583906
1109	20	0.9466392969157411
1109	21	0.8619534762430803
1109	22	1.2355679064854712
1109	23	1.5254816397070101
1109	24	1.268175243595337
1109	25	1.495810692334807
1110	1	0.5145925259916808
1110	2	0.795061463881769
1110	3	0.6824197506393475
1110	4	1.0885913162125327
1110	5	0.9523992121605183
1110	6	0.6073420146383413
1110	7	0.6451929165957679
1110	8	0.6197737755837345
1110	9	0.7641606689769276
1110	10	1.1837063327629807
1110	11	0.8185409700615836
1110	12	0.6717600992108501
1110	13	0.4396367526039029
1110	14	1.5215848051320933
1110	15	1.1313984769896663
1110	16	1.3494069560805428
1110	17	1.6023304913231513
1110	18	1.186064378217452
1110	19	0.7477756485064191
1110	20	0.5918238624666543
1110	21	0.5032749962570904
1110	22	0.8366622688983583
1110	23	-0.5950227931947936
1110	24	0.24140151963077727
1110	25	0.7794898082399797
1111	1	0.21092035947006238
1111	2	0.5925601525001696
1111	3	0.512736806945302
1111	4	0.8621935026433043
1111	5	0.6444529847669536
1111	6	0.361874924355682
1111	7	0.31402748580691053
1111	8	0.4048107625056865
1111	9	0.559809564435966
1111	10	0.8937519628818364
1111	11	0.563656371642395
1111	12	0.4197627756214857
1111	13	0.303836034920522
1111	14	1.127369190153729
1111	15	0.6685235410685451
1111	16	0.733595419498954
1111	17	1.0848678060905002
1111	18	1.3894019934104023
1111	19	0.7293309172800377
1111	20	0.4211678140170676
1111	21	0.17851626066738646
1111	22	0.6293772460684011
1111	23	1.011361846950173
1111	24	0.7887987251958037
1111	25	0.8028000632440921
1112	1	0.10678296237626483
1112	2	0.31833446007461674
1112	3	0.3098150693121404
1112	4	0.8407797167680648
1112	5	0.38788417517243445
1112	6	0.11224643477793705
1112	7	-0.06285392906448806
1112	8	0.2963591967343042
1112	9	0.4890968758109864
1112	10	0.7334492707881779
1112	11	0.22017580247969448
1112	12	0.22435936708354376
1112	13	0.1920310495064399
1112	14	0.9072827976041538
1112	15	0.6450733103640047
1112	16	0.5244483088580978
1112	17	1.0808618257396887
1112	18	1.2078364902898429
1112	19	0.7087822734334495
1112	20	0.4775450664744625
1112	21	0.4047157308829199
1112	22	0.770023106487784
1112	23	1.0362296503784236
1112	24	0.7570225747300299
1112	25	1.0395094416519441
1113	1	0.012469470749334022
1113	2	0.03988047282424034
1113	3	0.04561616469878871
1113	4	0.3088443479082521
1113	5	0.4407067028587939
1113	6	-0.027450499525521366
1113	7	-0.08375199232409258
1113	8	0.037631086575945716
1113	9	0.020719650922970345
1113	10	0.48644124528698995
1113	11	0.09698527198629137
1113	12	-0.06125325931296999
1113	13	-0.2612502397949541
1113	14	0.1655828077977759
1113	15	-0.01972070166447537
1113	16	0.09141711717157364
1113	17	0.428806130095829
1113	18	0.47119018018036485
1113	19	0.11136828800533038
1113	20	-0.04307731656256664
1113	21	-0.14245886589134216
1113	22	0.07693217143184228
1113	23	0.11654669636589432
1113	24	0.10911686398958409
1113	25	0.5507357574252651
1114	1	-1.6277179796277785
1114	2	-1.2371269796730182
1114	3	-1.3173226791383925
1114	4	-1.074515386768713
1114	5	-4.400011051956877
1114	6	-2.205852743463886
1114	7	-1.8020597876117748
1114	8	-1.385065059162823
1114	9	-1.1442154794768946
1114	10	-0.974836532853633
1114	11	-1.309392448595549
1114	12	-1.5848944776968814
1114	13	-1.6070797067088733
1114	14	-7.352919819860212
1114	15	-3.8322607788706122
1114	16	-2.4800236794272195
1114	17	-1.4448152516933916
1114	18	-7.496341944803746
1114	19	-3.7975974006309734
1114	20	-2.473169690283676
1114	21	-2.2680130387187383
1114	22	-1.970316269314683
1114	23	-1.3186501045917538
1114	24	-1.422080346929942
1114	25	-1.3251414194714428
1115	1	-1.3177265385803345
1115	2	-0.9088032687455403
1115	3	-0.9501004254110796
1115	4	-3.772882162860281
1115	5	-1.793773006417542
1115	6	-1.2255625577920775
1115	7	-1.1410356201191938
1115	8	-0.9035496175580526
1115	9	-0.7497392148907167
1115	10	-1.0917824702429613
1115	11	-1.243887355849779
1115	12	-1.3304966069569701
1115	13	-1.385327724047606
1115	14	-6.8050107167625775
1115	15	-3.512698070365854
1115	16	-2.221733719840948
1115	17	-1.233056475199227
1115	18	-1.1656238678377697
1115	19	-1.2491270270377655
1115	20	-1.2669346116475921
1115	21	-1.3595072650874442
1115	22	-2.738847302934957
1115	23	-1.6982514804837514
1115	24	-1.2309276524372272
1115	25	-0.918836224273619
1116	1	-1.539454799019353
1116	2	-1.4254243837477638
1116	3	-1.2570382584566566
1116	4	-1.3797596486880046
1116	5	-2.0075233180561654
1116	6	-1.777974932000749
1116	7	-1.6652595525675906
1116	8	-1.556631170182456
1116	9	-2.0269764307170632
1116	10	-1.3976007342468932
1116	11	-1.9464046969373818
1116	12	-1.991016711434547
1116	13	-2.1268459109282603
1116	14	-1.3533793613733849
1116	15	-1.6784047735670526
1116	16	-1.586536146777707
1116	17	-1.2299995041635619
1116	18	-1.3913399376727824
1116	19	-1.3242395081938199
1116	20	-2.3249040559172904
1116	21	-2.3270840330395792
1116	22	-2.3881950170802035
1116	23	-3.2772935208255163
1116	24	-3.10452586112676
1116	25	-2.348708796113602
1201	1	1.4743649715808118
1201	2	1.5649612501434724
1201	3	1.532202222655222
1201	4	1.971083327193238
1201	5	1.9409423774074348
1201	6	1.627941593513981
1201	7	1.5929362487332455
1201	8	1.6768890376242886
1201	9	1.933851842039978
1201	10	2.3589707265323057
1201	11	1.8120758377004762
1201	12	1.8163417503446198
1201	13	1.6352044800698877
1201	14	2.531077210962003
1201	15	2.322820648746905
1201	16	2.3648502519092105
1201	17	2.7131723863394654
1201	18	2.125229015898192
1201	19	2.1850250149069907
1201	20	2.1428209830270433
1201	21	1.9312196365220373
1201	22	2.256656508575439
1201	23	2.664051411747103
1201	24	2.1282251779555637
1201	25	2.6056294029529
1202	1	1.215665459090939
1202	2	1.359912132817989
1202	3	1.2940286517464656
1202	4	1.7450825080478403
1202	5	1.5774680287291964
1202	6	1.3181486196045284
1202	7	1.2741660273895548
1202	8	1.355376423552709
1202	9	1.6226695305568057
1202	10	1.9838473488623194
1202	11	1.5268954617448358
1202	12	1.4068513857691016
1202	13	1.4135480620238496
1202	14	2.439977079159135
1202	15	1.8915664143443323
1202	16	2.009310310198316
1202	17	2.364438468347501
1202	18	2.5258533526102127
1202	19	2.1392698137872586
1202	20	1.8063577793312575
1202	21	1.785714574775639
1202	22	1.983702889369198
1202	23	2.3727436328661256
1202	24	1.9229501327944192
1202	25	2.3982314176040163
1203	1	0.9275381344128704
1203	2	1.0165768781734315
1203	3	0.9898415411632371
1203	4	1.3700134059535678
1203	5	1.278800587141287
1203	6	1.0193258739297093
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2211	19	-0.7941625553873592
2211	20	-0.9401895987856506
2211	21	-0.9362182077343917
2211	22	-0.770772683162086
2211	23	-0.3125667609575302
2211	24	-0.7382402150611043
2211	25	-8.276130745571212
2212	1	-1.793383118497598
2212	2	-4.533888491644657
2212	3	-2.137603928329412
2212	4	-4.199949452236453
2212	5	-1.7088094119373594
2212	6	-1.2812247281896312
2212	7	-1.228354594620786
2212	8	-1.0708298418015794
2212	9	-1.2340993496581802
2212	10	-0.5361465495625097
2212	11	-1.1773719916065772
2212	12	-1.158825940164494
2212	13	-1.566926459520694
2212	14	-6.959904976517009
2212	15	-5.421180914756821
2212	16	-3.2231373702543102
2212	17	-7.410461286275758
2212	18	-3.406623230040587
2212	19	-2.30857908902399
2212	20	-2.3059409818154286
2212	21	-1.8858761700982356
2212	22	-1.6900458863015757
2212	23	-1.108276673117654
2212	24	-1.4160498803429462
2212	25	-1.1401231131074852
2213	1	0.014259080339289909
2213	2	-0.0648486260805683
2213	3	-0.061727542234720545
2213	4	0.39777874135910096
2213	5	0.15285690610328473
2213	6	-0.029812333416757042
2213	7	-0.13527925679298392
2213	8	-0.08189210783804768
2213	9	0.272734996426081
2213	10	-3.7051033267757845
2213	11	-1.2660079369195572
2213	12	-0.7613377625679689
2213	13	-0.491338876650552
2213	14	0.38113059283114126
2213	15	-0.018722595983597046
2213	16	0.14957207256984617
2213	17	0.6447001296134403
2213	18	0.762750826487057
2213	19	0.10299919456607398
2213	20	-0.19138655496426082
2213	21	-0.22123643451090658
2213	22	-0.42550580773362157
2213	23	-0.9859345126551524
2213	24	-0.33224947491589774
2213	25	0.009616918051413847
2214	1	-1.2110322961782458
2214	2	-0.9962044661557528
2214	3	-0.9278888130281437
2214	4	-0.4260903345535745
2214	5	-4.001269707151394
2214	6	-1.6586452429036171
2214	7	-1.2628444524780704
2214	8	-0.9285527746622009
2214	9	-0.8049541970669267
2214	10	-5.105109511523114
2214	11	-2.3168057533849478
2214	12	-1.562285654796515
2214	13	-1.2657078172320269
2214	14	-0.44316153255090285
2214	15	-0.8596579083085558
2214	16	-0.8579396856231085
2214	17	-0.5911566443904732
2214	18	-0.10662922272950937
2214	19	-0.8106942504625181
2214	20	-1.2362202346753293
2214	21	-1.0256060222592467
2214	22	-0.7403590204069985
2214	23	-0.5235207205410859
2214	24	-1.2036757509743232
2214	25	-0.7367532888092576
2215	1	-2.92396804792471
2215	2	-5.60888046489621
2215	3	-3.9820426862923206
2215	4	-2.780203722484483
2215	5	-2.751082429955492
2215	6	-3.0829226131637384
2215	7	-3.0547777219419814
2215	8	-3.3479094482176954
2215	9	-8.962625610071974
2215	10	-5.008348821480535
2215	11	-4.209058630204325
2215	12	-4.735041479163776
2215	13	-3.922544987006102
2215	14	-9.285148466457947
2215	15	-6.270499650062693
2215	16	-10.853057838480709
2215	17	-6.239038153597445
2215	18	-4.476062907642085
2215	19	-4.9021606188240305
2215	20	-4.562702289709357
2215	21	-4.77128098786158
2215	22	-10.869810408823433
2215	23	-12.251090117198093
2215	24	-8.742221580260054
2215	25	-6.968189982797021
2216	1	-3.6384602214179864
2216	2	-3.6298216719830236
2216	3	-3.1435297573342957
2216	4	-8.397807924433089
2216	5	-5.201743007187055
2216	6	-4.2216617107340895
2216	7	-3.762107889378271
2216	8	-9.759699716312426
2216	9	-6.629775835892939
2216	10	-4.776184804042607
2216	11	-4.2455723954995115
2216	12	-4.424873625699583
2216	13	-3.979819183422169
2216	14	-3.585278784077346
2216	15	-3.902391112967365
2216	16	-3.543934413193327
2216	17	-2.9839419102610734
2216	18	-9.768100520962292
2216	19	-7.280012185863605
2216	20	-6.538806090871688
2216	21	-5.665073617963163
2216	22	-5.312318188646252
2216	23	-4.353334321689481
2216	24	-4.772444937905437
2216	25	-4.190107876737152
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91033.0
3	2.0
4	786.0
5	4583.0
6	5424.0
7	5037.0
8	4530.0
9	4224.0
10	3935.0
11	3735.0
12	3502.0
13	3524.0
14	3548.0
15	3631.0
16	3665.0
17	3890.0
18	4367.0
19	5374.0
20	6890.0
21	8612.0
22	10673.0
23	12697.0
24	14333.0
25	16651.0
26	18488.0
27	20664.0
28	23174.0
29	27744.0
30	33141.0
31	39865.0
32	50441.0
33	64276.0
34	83827.0
35	110981.0
36	155550.0
37	222285.0
38	515376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.030997011767155	18.068134228018856	12.181095097597764	25.71977366261623
2	15.306573146519867	19.899515408204476	39.82354079020408	24.97037065507158
3	18.785567748211847	24.277366688677297	29.411786943832574	27.525278619278286
4	12.29892985351842	16.335589967581633	37.387730449683445	33.9777497292165
5	13.475223776404418	37.55234890406739	35.23623870407205	13.736188615456149
6	32.88141668605638	36.09593822121885	17.62198227884718	13.400662813877586
7	28.55132332161365	31.301664001388502	21.75451813384832	18.392494543149525
8	26.103839589692505	35.43247420800366	20.194098685592326	18.269587516711503
9	26.944112026230677	14.894362277928739	19.389082750020595	38.77244294581999
10	14.958819101497378	28.190096838154698	34.302742018351395	22.54834204199653
11	34.74948952095149	22.404920722462272	23.661324899869385	19.184264856716847
12	23.303714222644565	24.60742467188696	30.812092747709702	21.276768357758773
13	29.014267332299415	21.27339368302951	25.307754520881527	24.404584463789554
14	21.974635653220602	20.819052314751342	27.490242788845524	29.71606924318253
15	24.238282294258067	28.846801002644995	23.003217395953566	23.911699307143376
16	23.360688727062346	27.32363631790112	26.287024110478058	23.028650844558477
17	22.805814720721184	27.370713920445432	27.088544854251438	22.734926504581946
18	22.939365885197198	26.8517027715793	27.894724263457267	22.314207079766238
19	23.943740806216585	26.68028871703052	26.847954396947554	22.52801607980534
20	24.23975230827381	26.477493347655816	26.816666991605874	22.466087352464495
21	24.022428237188358	26.16267075901281	26.44984779491308	23.365053208885758
22	24.150337650835823	26.524901120135265	26.134099211980317	23.1906620170486
23	23.55260385712354	26.337682266854085	26.618051690360968	23.491662185661415
24	23.58382797343915	26.657914535247983	26.55204603612739	23.20621145518548
25	23.70161787912733	26.095573302069724	26.60585022777861	23.596958591024332
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	338.0
1	338.0
2	446.5
3	555.0
4	555.0
5	555.0
6	893.0
7	1231.0
8	1231.0
9	1231.0
10	1815.5
11	2400.0
12	2400.0
13	2400.0
14	4291.0
15	6182.0
16	6182.0
17	6182.0
18	10885.0
19	15588.0
20	15588.0
21	15588.0
22	24118.5
23	32649.0
24	32649.0
25	32649.0
26	46427.0
27	60205.0
28	60205.0
29	60205.0
30	78145.0
31	96085.0
32	96085.0
33	96085.0
34	112556.0
35	129027.0
36	129027.0
37	129027.0
38	145252.5
39	161478.0
40	161478.0
41	161478.0
42	174533.5
43	187589.0
44	187589.0
45	187589.0
46	200696.0
47	213803.0
48	213803.0
49	213803.0
50	210700.0
51	207597.0
52	207597.0
53	207597.0
54	190258.5
55	172920.0
56	172920.0
57	172920.0
58	151197.5
59	129475.0
60	129475.0
61	129475.0
62	107162.0
63	84849.0
64	84849.0
65	84849.0
66	66734.0
67	48619.0
68	48619.0
69	48619.0
70	36073.5
71	23528.0
72	23528.0
73	23528.0
74	16760.5
75	9993.0
76	9993.0
77	9993.0
78	7053.5
79	4114.0
80	4114.0
81	4114.0
82	2827.5
83	1541.0
84	1541.0
85	1541.0
86	1037.0
87	533.0
88	533.0
89	533.0
90	330.5
91	128.0
92	128.0
93	128.0
94	75.0
95	22.0
96	22.0
97	22.0
98	15.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16114854966305303
2	0.21056827655933072
3	0.21037965164751285
4	0.27161987301770935
5	0.2056640288520665
6	0.016850492122394933
7	0.016033117504517566
8	0.014838493063004492
9	0.01628461738694137
10	0.017542116799060398
11	0.017919366622696106
12	0.02395536380086742
13	0.024646988477532886
14	0.022131989653294836
15	0.041686105511745675
16	0.017479241828454447
17	0.03967410645235524
18	0.029048236419949473
19	0.024458363565715034
20	0.02621886274268167
21	0.031311735361763716
22	0.04086873089386831
23	0.02596736286025786
24	0.047156227954463434
25	0.03376385921539582
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1590458.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.96158243048895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.38689885847964	59.28691613396839
2	11.658162990114597	16.778797140024142
3	3.1303222545460647	6.7578882886353195
4	1.185238502913931	3.4116655290892064
5	0.5378306641332525	1.9351572835334834
6	0.30327802353790334	1.3094619894107158
7	0.1863833009497991	0.9388706092475918
8	0.11401629793846423	0.6563834578014385
9	0.08264505135386924	0.5352541807926108
>10	0.38536884452173265	4.756556174825074
>50	0.017371812085302783	0.8427213869037745
>100	0.01062439077441378	1.429049217128387
>500	0.0013278633221096637	0.6751289575724028
>1k	5.311453288438655E-4	0.6861496510674043
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2920	0.18359491416937762	No Hit
CCCATGTACTCTGCGTTGATACCAC	2612	0.16422942322274464	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1936	0.12172594309312161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.287497060595124E-5	0.0	0.0	0.0
3	0.0	6.287497060595124E-5	0.0	0.0	0.0
4	0.0	6.287497060595124E-5	0.0	0.0	0.0
5	0.0	6.287497060595124E-5	0.0	0.0	0.0
6	0.0	6.287497060595124E-5	0.0	0.0	0.0
7	0.0	6.287497060595124E-5	0.0	0.0	0.0
8	6.287497060595124E-5	6.287497060595124E-5	0.0	0.0	0.0
9	6.287497060595124E-5	6.287497060595124E-5	0.0	0.0	0.0
10	6.287497060595124E-5	6.287497060595124E-5	0.0	6.287497060595124E-5	0.0
11	6.287497060595124E-5	6.287497060595124E-5	0.0	6.287497060595124E-5	0.0
12	6.287497060595124E-5	6.287497060595124E-5	0.0	6.287497060595124E-5	0.0
13	6.287497060595124E-5	6.287497060595124E-5	0.0	6.287497060595124E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTAT	50	0.0015108621	13.286769	8
CTAAGAC	135	4.5965862E-9	11.279274	3
CGCGGTT	60	0.0059069847	11.075443	18
GTCCTAA	280	0.0	10.8998165	1
TATACTG	280	0.0	10.868901	5
GTAGACC	265	0.0	10.773835	3
GTAGGAC	505	0.0	10.741833	3
TGCACCG	80	3.733238E-4	10.699076	5
GTATTAA	215	0.0	10.646332	1
AGGACCG	145	1.4186298E-8	10.494112	5
TAGGACC	210	0.0	10.4226	4
GTCTAGC	110	5.7678426E-6	10.40437	1
TGTAGGA	660	0.0	10.387956	2
GTCTTAC	130	3.638761E-7	10.270981	1
ATTATAC	130	3.7476093E-7	10.248956	3
GGACGTG	495	0.0	10.160641	6
GCTAGAC	150	2.3770554E-8	10.151346	3
TTTATAC	245	0.0	10.099554	3
TAGAACA	370	0.0	10.030682	4
TAACACT	190	9.640644E-11	10.017144	4
>>END_MODULE