FastQCFastQC Report
Thu 26 May 2016
SRR1513204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590458
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC33910.21320902532478064No Hit
GTACATGGGAAGCAGTGGTATCAAC33260.20912215223539382No Hit
GTATCAACGCAGAGTACTTTTTTTT29470.1852925383757383No Hit
TATCAACGCAGAGTACTTTTTTTTT23160.14561843192338306No Hit
GGTATCAACGCAGAGTACTTTTTTT18710.11763907000373477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAAT802.794801E-511.90440751
CCACGAC657.8817277E-411.71719553
TCTACAC2550.011.2002593
GTATTAC1252.16447E-710.6663481
CCCCTAT1409.340329E-810.2037771
TAATACT1409.400901E-810.1992724
GTCCTAA3750.010.1584271
TCTATAC1605.9571903E-910.1152343
TAGCACC1605.9662852E-910.1142784
TAGGACT1701.5043042E-910.079284
GACAGTC1701.5825208E-910.0497997
GCAATAC1054.009572E-59.9735643
AGGACGT5600.09.6893085
TAGGACG5600.09.6893084
GTTCTAA1401.0229924E-69.5235261
CACTTAC1401.0229924E-69.5235261
CTTACAC2303.6379788E-129.5202213
TTAGACC1002.6992738E-49.519324
GGACGTG5700.09.5184196
GCCGTTT800.00454791879.491775515
Produced by FastQC (version 0.11.5)