FastQCFastQC Report
Thu 26 May 2016
SRR1513203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167424
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23910.11031528671824249No Hit
GTACATGGGAAGCAGTGGTATCAAC22470.10367145514675485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT1451.376975E-810.5148344
TAGAACC1451.376975E-810.5148344
GCGCGCT750.002672243910.12233911
GTGCGCG856.668391E-410.046759
ACCGTGC856.6783023E-410.0451278
AGGACGT4750.010.010415
TAGGACC3050.09.9977114
GAACCGT951.6676536E-49.9861536
GGACCGT1054.1692245E-59.9386016
GTATAAG2600.09.91902351
TAGGACG5000.09.9102314
GTAGAAC3100.09.8364583
CCTATAC1659.726136E-99.8178273
GTCCTAT1855.875336E-109.8098051
GTATTGG2750.09.7253181
GGACGTG4750.09.5867076
GTGTAGC3200.09.5516521
CCCTATA1304.043046E-69.5354792
TTAGGAC3600.09.5290683
ATTATAC3300.09.5290683
Produced by FastQC (version 0.11.5)