##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513203_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2167424 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.5270989893994 31.0 30.0 34.0 25.0 34.0 2 29.363641816275912 31.0 30.0 34.0 22.0 34.0 3 29.568918679501564 31.0 30.0 34.0 24.0 34.0 4 32.65461718611587 37.0 33.0 37.0 23.0 37.0 5 32.796236453965626 37.0 33.0 37.0 25.0 37.0 6 33.14795766771984 37.0 35.0 37.0 27.0 37.0 7 33.12373951751019 37.0 35.0 37.0 27.0 37.0 8 32.99887285551881 37.0 35.0 37.0 26.0 37.0 9 34.241471903974485 39.0 35.0 39.0 25.0 39.0 10 33.831310348136775 39.0 34.0 39.0 19.0 39.0 11 34.278669055985354 39.0 35.0 39.0 26.0 39.0 12 34.272049216027874 39.0 35.0 39.0 26.0 39.0 13 34.303784123457156 39.0 35.0 39.0 26.0 39.0 14 34.536559067353686 40.0 34.0 41.0 17.0 41.0 15 34.95899464064253 40.0 34.0 41.0 23.0 41.0 16 34.80579157562157 40.0 34.0 41.0 19.0 41.0 17 34.404598269650975 39.0 34.0 41.0 17.0 41.0 18 34.1292183716707 39.0 33.0 41.0 17.0 41.0 19 34.77146049873029 39.0 34.0 41.0 19.0 41.0 20 34.98103139948621 40.0 35.0 41.0 24.0 41.0 21 35.03164124785921 40.0 35.0 41.0 24.0 41.0 22 34.715050216293626 40.0 35.0 41.0 18.0 41.0 23 34.24643217017067 39.0 34.0 41.0 11.0 41.0 24 34.49101652468553 39.0 34.0 41.0 18.0 41.0 25 34.1241183081852 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9184097963852551 1101 2 1.1548767967424638 1101 3 1.0123593563373312 1101 4 1.4086079139220686 1101 5 1.2417481994060964 1101 6 1.01924642066772 1101 7 0.9416477711080518 1101 8 0.9414373177087398 1101 9 1.3303385906612988 1101 10 1.585396848920631 1101 11 1.214169187860854 1101 12 1.1365237886301216 1101 13 1.0082093655945314 1101 14 2.056324512090626 1101 15 1.6506459743889366 1101 16 1.7094692321612044 1101 17 2.067337527692011 1101 18 2.0402161836574777 1101 19 1.5579653282095336 1101 20 1.2549301828292556 1101 21 1.2951887165699247 1101 22 1.6116918450187043 1101 23 1.9135811307687547 1101 24 1.6283651702317954 1101 25 1.7696982307502651 1102 1 0.9739107096914239 1102 2 1.2277017061911195 1102 3 1.1183412359976757 1102 4 1.4311374435154391 1102 5 1.4257540959735095 1102 6 1.0791167211212311 1102 7 1.1348317113060418 1102 8 1.1338347931568933 1102 9 1.4888707021609022 1102 10 1.6872690666217451 1102 11 1.4385429772925917 1102 12 1.23820082879881 1102 13 1.1130482817885579 1102 14 2.163071468496021 1102 15 1.74811475677366 1102 16 1.854945165620535 1102 17 2.289351155591561 1102 18 2.470698310526714 1102 19 1.8630005728600096 1102 20 1.6410080303711467 1102 21 1.6157270996463566 1102 22 1.8410355162073841 1102 23 2.1753452767040073 1102 24 2.0445166625246785 1102 25 2.2664552625363896 1103 1 0.9548971185868638 1103 2 1.2179945663448777 1103 3 0.9630232706774997 1103 4 1.561352536437532 1103 5 1.3407960717352907 1103 6 1.082905579646237 1103 7 0.956192465808769 1103 8 0.9310390535318334 1103 9 1.2865683677101885 1103 10 1.5823078689910872 1103 11 1.2813806209913494 1103 12 1.1870334122748005 1103 13 1.2084179007616243 1103 14 2.1277425301200523 1103 15 1.6571247061236392 1103 16 1.7002381739296553 1103 17 2.0561118770221896 1103 18 2.3209239368176924 1103 19 1.8078539898485602 1103 20 1.4269199840243232 1103 21 1.5012121746655396 1103 22 1.6241427453291877 1103 23 2.0125266351262567 1103 24 1.7190874659760311 1103 25 1.9412891134576782 1104 1 0.9013835312779364 1104 2 1.1397814070755317 1104 3 0.9754375198784508 1104 4 1.3692573602421803 1104 5 1.2180763276286442 1104 6 1.0580636949201434 1104 7 0.9659109371702996 1104 8 1.0059598416198625 1104 9 1.2697753664222518 1104 10 1.4191632296632832 1104 11 1.0417936147259823 1104 12 0.9537241173619293 1104 13 0.8832792763554878 1104 14 1.8740273504651341 1104 15 1.513776446564144 1104 16 1.6458856153663888 1104 17 2.0693659519237 1104 18 2.151431236731547 1104 19 1.5321958716315578 1104 20 1.4039605392700594 1104 21 1.2456036371148116 1104 22 1.594946127948873 1104 23 1.9307093267419901 1104 24 1.6507912944077887 1104 25 1.8439563907685752 1105 1 0.6053307245596073 1105 2 0.834160572532177 1105 3 0.7689380087274884 1105 4 1.077620027475767 1105 5 1.0364084981921735 1105 6 0.7124709163044471 1105 7 0.6198954888494441 1105 8 0.7872433545403368 1105 9 1.024550343142728 1105 10 1.3225336492664113 1105 11 0.7578855798075779 1105 12 0.8003310697508255 1105 13 0.8133470090589796 1105 14 1.5625837620189529 1105 15 1.2134676127859834 1105 16 1.384840321893968 1105 17 1.6761424541782333 1105 18 1.3472595754789438 1105 19 1.153505766614245 1105 20 0.9695016653125919 1105 21 0.9649044358718228 1105 22 1.0039267523025543 1105 23 1.383402872337406 1105 24 1.2212057787988542 1105 25 1.4461148178357277 1106 1 0.5110823869525838 1106 2 0.649163397513707 1106 3 0.508069652866741 1106 4 1.0042487379803546 1106 5 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1107 18 2.045657252024334 1107 19 1.3833967065932882 1107 20 1.2301579809986123 1107 21 1.1041506717616443 1107 22 1.2830901204108613 1107 23 1.6453671978279587 1107 24 1.434689971032988 1107 25 1.6626889966949676 1108 1 0.5831249262166658 1108 2 0.8561316230865827 1108 3 0.6799088214500948 1108 4 1.1256044528489184 1108 5 1.0801237429566513 1108 6 0.676872520789253 1108 7 0.631730659619393 1108 8 0.6547439200938427 1108 9 1.005043690836672 1108 10 1.2477631224862051 1108 11 0.8065525697513394 1108 12 0.6086765531870455 1108 13 0.5908012980951867 1108 14 1.6187067154363959 1108 15 1.1378455356816772 1108 16 1.1794433541315144 1108 17 1.4256670173414463 1108 18 1.7259364215643984 1108 19 1.005499644304848 1108 20 0.8881542093781505 1108 21 0.8592008084016811 1108 22 1.007199887522539 1108 23 1.3235572434990601 1108 24 1.1262473050295583 1108 25 1.4077467975233304 1109 1 0.3441858661029755 1109 2 0.4759060505141761 1109 3 0.4062213136501356 1109 4 0.6700967459132556 1109 5 0.7968547354237785 1109 6 0.35735640844997363 1109 7 0.2822552517448216 1109 8 0.3435211050008249 1109 9 0.4866298424069697 1109 10 0.7920977742898572 1109 11 0.49052853087927417 1109 12 0.3667964539227597 1109 13 0.29710808467572747 1109 14 1.2141192659885576 1109 15 0.7899326047844681 1109 16 0.9945637961377258 1109 17 1.3208136547805722 1109 18 1.429293204035261 1109 19 0.6810006124316672 1109 20 0.7198207704180675 1109 21 0.5455625137889868 1109 22 0.8640993466603462 1109 23 1.1077300254217235 1109 24 0.8022763181224803 1109 25 1.0335968043491306 1110 1 0.311928190494843 1110 2 0.5685671389097635 1110 3 0.4787161627269647 1110 4 0.7592159668713805 1110 5 0.7005483783226154 1110 6 0.38669315223580014 1110 7 0.3439536004133643 1110 8 0.4041659927497321 1110 9 0.59214322442309 1110 10 0.8831862833993327 1110 11 0.4881832141163116 1110 12 0.4091623734983969 1110 13 0.31199848329157476 1110 14 1.193387883738886 1110 15 0.9055933032472794 1110 16 1.0366527763169842 1110 17 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-9.483830666251112 2215 17 -4.744997487415603 2215 18 -3.1177400011098655 2215 19 -3.597352308583069 2215 20 -3.4735914047050365 2215 21 -3.527262965738906 2215 22 -9.491465949071852 2215 23 -10.871564071497588 2215 24 -6.825068274792375 2215 25 -5.137023855135116 2216 1 -1.7966534575129494 2216 2 -1.7413282036575062 2216 3 -1.6596803856599962 2216 4 -6.853836604826505 2216 5 -3.412767901662246 2216 6 -2.647352719613451 2216 7 -2.245632600787445 2216 8 -8.054678534474245 2216 9 -3.9199104285744326 2216 10 -2.0958337613003373 2216 11 -2.071050466183152 2216 12 -2.2631810365526803 2216 13 -2.0927741265052973 2216 14 -1.3183090296624371 2216 15 -1.8776018941369443 2216 16 -1.6646070286409227 2216 17 -1.3417367299110268 2216 18 -7.615827168449361 2216 19 -4.68561447726762 2216 20 -3.9510548304969575 2216 21 -3.125799471416837 2216 22 -2.751745621197184 2216 23 -2.0669024886856704 2216 24 -2.426577125250276 2216 25 -1.8260999545555237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94043.0 3 3.0 4 834.0 5 4763.0 6 5834.0 7 5344.0 8 4890.0 9 4645.0 10 4416.0 11 4168.0 12 4080.0 13 4154.0 14 4203.0 15 4131.0 16 4258.0 17 4604.0 18 5105.0 19 6479.0 20 8204.0 21 10304.0 22 12849.0 23 15209.0 24 17860.0 25 20350.0 26 22799.0 27 25779.0 28 28799.0 29 34142.0 30 41683.0 31 49314.0 32 63274.0 33 82392.0 34 108102.0 35 144806.0 36 204277.0 37 297115.0 38 814212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46917336596565 17.724552060438796 11.921486495729024 25.884788077866528 2 15.26780584873875 19.968851502056985 39.56534570022138 25.197996948982887 3 18.511412158321004 24.377978047617308 29.442179677601843 27.668430116459845 4 12.00804234746445 16.189302917761065 37.70147002728521 34.101184707489274 5 13.465552801368757 37.29257835825901 35.3758462802983 13.866022560073931 6 33.00681855518249 35.95901852675646 17.570192909367115 13.463970008693934 7 28.712564709096327 31.15381314126495 21.880373196936233 18.253248952702485 8 26.229620913223044 34.90380932604232 20.38513186128608 18.481437899448558 9 26.94004801032194 14.615875893040903 19.341874654481657 39.1022014421555 10 14.514472201430376 27.967375824995315 34.75164999063213 22.766501982942174 11 35.246580981229314 22.003409327935998 23.554635080083212 19.195374610751472 12 23.46147118940365 24.23641778260607 30.636511189699007 21.66559983829128 13 29.203957208540753 20.887072248083765 25.381643015929345 24.52732752744613 14 21.43350934466008 20.406376649471643 27.92566913377876 30.234444872089515 15 24.188304495203493 28.427804978916036 23.053980105013625 24.32991042086684 16 23.584714977129504 26.885708431626632 25.985055727222722 23.544520864021145 17 22.606781194324697 27.09057593072954 27.199595676134734 23.10304719881103 18 22.705189379132552 26.652316720902014 27.907167788679203 22.73532611128623 19 24.133187193539083 26.334237092587255 26.652483415056246 22.880092298817424 20 24.511656284295743 26.215448118758694 26.426356853887782 22.846538743057778 21 24.400394145053685 25.859515441578612 26.131493989749462 23.60859642361824 22 24.16868716660597 26.05976433424123 26.211388373090216 23.56016012606258 23 23.534664774497248 25.9498575104127 26.69851049346394 23.81696722162611 24 23.9656268275807 26.21455214666158 26.28914398024055 23.530677045517173 25 23.799855817469716 25.87310275820627 26.48804771131002 23.838993713013995 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 244.0 1 244.0 2 387.0 3 530.0 4 530.0 5 530.0 6 1040.5 7 1551.0 8 1551.0 9 1551.0 10 2191.5 11 2832.0 12 2832.0 13 2832.0 14 5046.5 15 7261.0 16 7261.0 17 7261.0 18 13340.0 19 19419.0 20 19419.0 21 19419.0 22 30725.5 23 42032.0 24 42032.0 25 42032.0 26 60246.0 27 78460.0 28 78460.0 29 78460.0 30 101898.5 31 125337.0 32 125337.0 33 125337.0 34 148745.5 35 172154.0 36 172154.0 37 172154.0 38 196401.0 39 220648.0 40 220648.0 41 220648.0 42 239266.0 43 257884.0 44 257884.0 45 257884.0 46 273420.0 47 288956.0 48 288956.0 49 288956.0 50 286304.5 51 283653.0 52 283653.0 53 283653.0 54 262596.0 55 241539.0 56 241539.0 57 241539.0 58 210710.0 59 179881.0 60 179881.0 61 179881.0 62 149783.5 63 119686.0 64 119686.0 65 119686.0 66 94217.5 67 68749.0 68 68749.0 69 68749.0 70 50873.5 71 32998.0 72 32998.0 73 32998.0 74 23788.5 75 14579.0 76 14579.0 77 14579.0 78 10279.0 79 5979.0 80 5979.0 81 5979.0 82 4026.5 83 2074.0 84 2074.0 85 2074.0 86 1405.5 87 737.0 88 737.0 89 737.0 90 469.5 91 202.0 92 202.0 93 202.0 94 115.0 95 28.0 96 28.0 97 28.0 98 19.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16222022087048957 2 0.2547724856788519 3 0.2566641321679561 4 0.45422584598122007 5 0.2520503602433119 6 0.018270536821590976 7 0.019285566645012697 8 0.017255506998169256 9 0.017993710506112325 10 0.0203928719069273 11 0.019746943837477115 12 0.02606781137423965 13 0.02505278155081793 14 0.023899338569656883 15 0.0391247859209827 16 0.020300596468434417 17 0.038940235043996935 18 0.029205176282997698 19 0.02532960786629658 20 0.028144008740329535 21 0.03271164294572727 22 0.040647330656115276 23 0.027636493828618672 24 0.04461517451130928 25 0.03349598417291679 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2167424.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.99035773890643 #Duplication Level Percentage of deduplicated Percentage of total 1 82.07163676697571 58.262948543051905 2 11.691414343596001 16.59955373451324 3 3.1021633626729055 6.6067106064203545 4 1.207147280776625 3.4278326922352296 5 0.5942941571769591 2.1094577410067106 6 0.34818492543230023 1.4830663449440071 7 0.21278567604624973 1.0574011884966823 8 0.1558914960130709 0.8853434456336967 9 0.10789671166598974 0.6893663544018225 >10 0.47755628676096 5.6751537093133715 >50 0.019697757648277598 0.947943818771643 >100 0.00975219392029246 1.2054361525406934 >500 0.0011842809860054234 0.5748225762738162 >1k 3.947603286684039E-4 0.4749630923968168 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2391 0.11031528671824249 No Hit GTACATGGGAAGCAGTGGTATCAAC 2247 0.10367145514675485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.3841315773932558E-4 0.0 0.0 0.0 4.613771924644186E-5 7 1.3841315773932558E-4 0.0 0.0 0.0 4.613771924644186E-5 8 1.3841315773932558E-4 0.0 0.0 0.0 4.613771924644186E-5 9 1.3841315773932558E-4 0.0 0.0 4.613771924644186E-5 4.613771924644186E-5 10 1.8455087698576744E-4 0.0 0.0 4.613771924644186E-5 4.613771924644186E-5 11 1.8455087698576744E-4 0.0 0.0 4.613771924644186E-5 4.613771924644186E-5 12 2.306885962322093E-4 0.0 0.0 4.613771924644186E-5 9.227543849288372E-5 13 2.306885962322093E-4 0.0 0.0 4.613771924644186E-5 9.227543849288372E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGACT 145 1.376975E-8 10.514834 4 TAGAACC 145 1.376975E-8 10.514834 4 GCGCGCT 75 0.0026722439 10.122339 11 GTGCGCG 85 6.668391E-4 10.04675 9 ACCGTGC 85 6.6783023E-4 10.045127 8 AGGACGT 475 0.0 10.01041 5 TAGGACC 305 0.0 9.997711 4 GAACCGT 95 1.6676536E-4 9.986153 6 GGACCGT 105 4.1692245E-5 9.938601 6 GTATAAG 260 0.0 9.9190235 1 TAGGACG 500 0.0 9.910231 4 GTAGAAC 310 0.0 9.836458 3 CCTATAC 165 9.726136E-9 9.817827 3 GTCCTAT 185 5.875336E-10 9.809805 1 GTATTGG 275 0.0 9.725318 1 GGACGTG 475 0.0 9.586707 6 GTGTAGC 320 0.0 9.551652 1 CCCTATA 130 4.043046E-6 9.535479 2 TTAGGAC 360 0.0 9.529068 3 ATTATAC 330 0.0 9.529068 3 >>END_MODULE