FastQCFastQC Report
Thu 26 May 2016
SRR1513203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167424
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33530.15469977263331955No Hit
GTACATGGGAAGCAGTGGTATCAAC24980.11525202267761175No Hit
TATCAACGCAGAGTACTTTTTTTTT24530.11317582531152187No Hit
CCCATGTACTCTGCGTTGATACCAC23940.1104536998759818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC1352.7284841E-1112.6994931
TAGGACC3550.011.2638684
GTAGGAC5600.011.05075553
GTCCTAC6600.010.9677451
GACGTGA3650.010.9206327
TGTAGGA7000.010.8817522
TTAGATA1053.3786018E-610.8807434
CGTGAAA4050.010.7761569
ATAAGCC1803.45608E-1110.5785013
GGACGTG5850.010.4143096
AGGACGT5800.010.3413975
TAGGACG5850.010.2530094
TGGCGAG6600.010.210237518
GTCCTAA4200.010.204951
TCTATAC2151.8189894E-1210.1848823
GTCTTAT1701.4915713E-910.0848921
GTTCTAG2700.09.8773841
TTAGGAC5450.09.7826883
GTATAAG2054.0017767E-119.75692751
TATGGCG7100.09.6253316
Produced by FastQC (version 0.11.5)