FastQCFastQC Report
Thu 26 May 2016
SRR1513202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697269
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC26960.15884341256453752No Hit
CCCATGTACTCTGCGTTGATACCAC24090.14193389498070136No Hit
GTATCAACGCAGAGTACTTTTTTTT21700.12785245002412698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT508.802448E-515.1821536
TGAACCG604.0506304E-412.6820675
TAGGACC3750.011.9363044
TTATACT2000.011.4283764
ATTTGCG752.0916208E-411.38963616
TCGAACT1053.5017165E-610.84791219
CTACACT2650.010.7814874
GTAGGAC6900.010.7664993
GTCCTAA4800.010.7382411
TAGGACA3250.010.5492714
GGCGAGG4250.010.4969519
GTCCTAG2100.010.454161
GTCTTAG2600.010.2793421
TTGAACC1303.7119025E-710.2562344
TTAGGAC5300.010.2430183
GTCCTAC7150.010.14584351
GTGCGCC750.002670446610.12292811
CTTACAC2650.010.0633163
TGGCGAG8400.010.05691818
GTATAAA2850.010.0474771
Produced by FastQC (version 0.11.5)