FastQCFastQC Report
Thu 26 May 2016
SRR1513202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697269
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33780.19902561114354886No Hit
GTACATGGGAAGCAGTGGTATCAAC31310.18447282074909752No Hit
CCCATGTACTCTGCGTTGATACCAC29520.1739264665766004No Hit
TATCAACGCAGAGTACTTTTTTTTT25690.15136080373824068No Hit
GGTATCAACGCAGAGTACTTTTTTT21750.1281470409228001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG8200.011.2611624
AGGACGT8300.011.240185
GGACGTG8250.011.1912586
CGTGAAA5800.011.1242539
TTAGGAC6650.011.0228623
GACGTGA5350.010.9987427
TAGGACC3400.010.9197074
ACGTGAA6400.010.8235878
ACACCGT803.704442E-410.708136
GTACTAG909.269735E-510.5828111
GGCGAGG4800.010.47701119
GTAGGAC8050.010.1701623
TGTAGGA8200.09.9850082
TTAGACT1252.5022164E-69.9005344
ACAATAC1659.8752935E-99.8082223
TGGCGAG8550.09.76922418
TACACCG800.0044391139.5197455
TTTAGGA7400.09.39193252
CTTTAGG7000.09.3884661
GTCCTAC6300.09.3733471
Produced by FastQC (version 0.11.5)