##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513202_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1697269 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.537339690997715 31.0 31.0 34.0 27.0 34.0 2 30.657261754029562 31.0 31.0 34.0 27.0 34.0 3 30.74387913760282 33.0 31.0 34.0 27.0 34.0 4 34.12225581213114 37.0 35.0 37.0 30.0 37.0 5 33.86902901072252 37.0 35.0 37.0 30.0 37.0 6 33.78292892876733 37.0 35.0 37.0 29.0 37.0 7 33.63392779812746 37.0 35.0 37.0 28.0 37.0 8 33.59680580980387 37.0 35.0 37.0 28.0 37.0 9 35.125666585555976 39.0 35.0 39.0 28.0 39.0 10 34.965370839860974 39.0 35.0 39.0 27.0 39.0 11 34.968339726937806 39.0 35.0 39.0 27.0 39.0 12 34.907190315736635 39.0 35.0 39.0 27.0 39.0 13 34.874777657519225 39.0 35.0 39.0 27.0 39.0 14 36.00984699537905 40.0 36.0 41.0 27.0 41.0 15 35.97753862233977 40.0 36.0 41.0 27.0 41.0 16 35.933822511340274 40.0 36.0 41.0 27.0 41.0 17 35.86238834268463 40.0 36.0 41.0 27.0 41.0 18 35.822749958904566 40.0 36.0 41.0 27.0 41.0 19 35.90008419407884 40.0 36.0 41.0 27.0 41.0 20 35.86714127224382 40.0 36.0 41.0 27.0 41.0 21 35.81254297344734 40.0 36.0 41.0 27.0 41.0 22 35.74780014246416 40.0 36.0 41.0 27.0 41.0 23 35.66492877675843 40.0 36.0 41.0 27.0 41.0 24 35.59347163001269 40.0 36.0 41.0 26.0 41.0 25 35.53495114799127 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6445085021742543 1101 2 0.7063557012714909 1101 3 0.6435350678288856 1101 4 0.8010991685742752 1101 5 0.6380572614789557 1101 6 0.6313018024871582 1101 7 0.7531249728242173 1101 8 0.6903185916911312 1101 9 0.7883457031351782 1101 10 0.7903993707741037 1101 11 0.7587673682252429 1101 12 0.6938171380940474 1101 13 0.764822384055627 1101 14 0.8323315844130548 1101 15 0.974055444962417 1101 16 0.8365623544273078 1101 17 1.015155011084829 1101 18 0.9561858891605155 1101 19 0.8122582988975822 1101 20 0.9656887747887097 1101 21 0.9201354618917819 1101 22 0.9562844609681349 1101 23 1.0687429027214534 1101 24 0.9374960000052823 1101 25 1.053623350986662 1102 1 0.7968128572162811 1102 2 0.7034961073735104 1102 3 0.6732553058216979 1102 4 0.7443371941375787 1102 5 0.8751786119477458 1102 6 0.891781984462078 1102 7 0.9117138568346377 1102 8 0.9039147277215989 1102 9 0.9191736157246098 1102 10 0.9648175300765303 1102 11 0.930493843010801 1102 12 1.0016875919822468 1102 13 1.0518903920730907 1102 14 1.214723274536155 1102 15 1.060737899736509 1102 16 1.2172279083589714 1102 17 1.3637540362667977 1102 18 1.317429878420981 1102 19 1.242741872364455 1102 20 1.2749528765401266 1102 21 1.270625757284627 1102 22 1.260724567830664 1102 23 1.2717750408545356 1102 24 1.2380547635705526 1102 25 1.3121664692831203 1103 1 0.5974037968947279 1103 2 0.5072308593737489 1103 3 0.5220176433691108 1103 4 0.5825624949900003 1103 5 0.631551765157873 1103 6 0.5885664687392946 1103 7 0.648748456689134 1103 8 0.6804877848309729 1103 9 0.7706173186304852 1103 10 0.7244892878408891 1103 11 0.751084407769504 1103 12 0.8553736457672443 1103 13 0.6685055945724798 1103 14 0.8229238720369239 1103 15 0.839545726627243 1103 16 0.7766241552469282 1103 17 0.8022123387128985 1103 18 0.800101143000461 1103 19 0.7221079161589401 1103 20 0.8837803619065383 1103 21 0.8783716267579749 1103 22 0.8796499375745839 1103 23 0.8221558462869041 1103 24 0.9584076402387751 1103 25 0.9517466087918649 1104 1 0.558600686005331 1104 2 0.7084925591351876 1104 3 0.576808255217589 1104 4 0.7415428631186458 1104 5 0.6502409386172872 1104 6 0.703085217710246 1104 7 0.6970812125261503 1104 8 0.8034684249153301 1104 9 0.7835075006742542 1104 10 0.9118793348624621 1104 11 0.9481991924671362 1104 12 0.9237504387704405 1104 13 0.8964341084464564 1104 14 0.9325255743267888 1104 15 0.8833311426471298 1104 16 1.0211639480199537 1104 17 0.9168606489149767 1104 18 0.95346873838497 1104 19 0.994214625464771 1104 20 1.043463418819826 1104 21 1.0386115648743939 1104 22 1.0443629464778539 1104 23 1.117725562106834 1104 24 1.0497266008764612 1104 25 1.0944738003494408 1105 1 0.6112352783893797 1105 2 0.7028479951686286 1105 3 0.5031282323046931 1105 4 0.6335868556638715 1105 5 0.6323918355585576 1105 6 0.6649677395406783 1105 7 0.643622413303099 1105 8 0.6083139474298633 1105 9 0.636304805876243 1105 10 0.6206332251914901 1105 11 0.7103069364279122 1105 12 0.594927475651204 1105 13 0.644278412837707 1105 14 0.6942686357257486 1105 15 0.7143642949459519 1105 16 0.5900590447302108 1105 17 0.7782365489345651 1105 18 0.5558439036507039 1105 19 0.6004905604302948 1105 20 0.6530443989604535 1105 21 0.7094326758858145 1105 22 0.5552848322005914 1105 23 0.5768257459434878 1105 24 0.5761829937604261 1105 25 0.5459368011682599 1106 1 0.44256823709968884 1106 2 0.5222955493947659 1106 3 0.5126949775190113 1106 4 0.6110181735688229 1106 5 0.5907393893499346 1106 6 0.6366367829259332 1106 7 0.5187645273073329 1106 8 0.718125139600609 1106 9 0.7623705250437496 1106 10 0.656867948615357 1106 11 0.6247907174973548 1106 12 0.6878561762672675 1106 13 0.6193291173753579 1106 14 0.7418958481391797 1106 15 0.7108948654888394 1106 16 0.7755460800201348 1106 17 0.7085987847601203 1106 18 0.7969820221591064 1106 19 0.7955098314153446 1106 20 0.8340275344029848 1106 21 0.9514389669439964 1106 22 0.769642205172957 1106 23 0.5895766056924572 1106 24 0.7127686817117649 1106 25 0.6944214800286517 1107 1 0.40432859212927497 1107 2 0.4868083449857359 1107 3 0.40698529217519663 1107 4 0.5969088449635223 1107 5 0.494729466892494 1107 6 0.35207426823984633 1107 7 0.46900457814790997 1107 8 0.38130604020513914 1107 9 0.5917736822864015 1107 10 0.49189320869268727 1107 11 0.596454571554986 1107 12 0.5602423190053969 1107 13 0.6353377581375383 1107 14 0.6338267754456979 1107 15 0.6327819481368238 1107 16 0.5742400859586567 1107 17 0.6307520861934819 1107 18 0.8475045799956078 1107 19 0.7545631392571863 1107 20 0.6027402851346153 1107 21 0.6454037844092255 1107 22 0.7280912471057519 1107 23 0.5590367449700082 1107 24 0.6389240765807145 1107 25 0.6479800535018327 1108 1 0.3862554302314116 1108 2 0.46578289640264714 1108 3 0.42222214587624407 1108 4 0.6643326188538481 1108 5 0.4248080367613625 1108 6 0.33283504528264274 1108 7 0.34029055338055514 1108 8 0.47780066557631784 1108 9 0.467195657006485 1108 10 0.5019731383995278 1108 11 0.5054938329537606 1108 12 0.5619907568261553 1108 13 0.5122620923072603 1108 14 0.5839709313458883 1108 15 0.5610959974858645 1108 16 0.6568681558909404 1108 17 0.5905573678564622 1108 18 0.5587119984347453 1108 19 0.6189429681529575 1108 20 0.5776538896597359 1108 21 0.581157585744748 1108 22 0.5121790507974495 1108 23 0.45421206600290986 1108 24 0.6286844966660396 1108 25 0.5538727282900453 1109 1 0.3868322524513843 1109 2 0.47509742850450465 1109 3 0.38704142592363056 1109 4 0.5328518866515495 1109 5 0.4872047960572061 1109 6 0.4482374049641109 1109 7 0.46191095318688724 1109 8 0.4829012535080963 1109 9 0.3767306834122479 1109 10 0.45096129332673485 1109 11 0.5270947711416483 1109 12 0.47098913042877655 1109 13 0.4683110496788885 1109 14 0.6402618067103987 1109 15 0.5421626797783929 1109 16 0.5121842799213709 1109 17 0.5238646021165465 1109 18 0.5806044631824392 1109 19 0.42859123784538156 1109 20 0.4833318566152869 1109 21 0.5528959484127967 1109 22 0.5814312579942182 1109 23 0.5805108108610924 1109 24 0.4940844700455145 1109 25 0.5776794049508922 1110 1 0.15480688499167528 1110 2 0.1705764054889194 1110 3 0.10572560611382542 1110 4 0.008819811564038105 1110 5 0.15073635239707528 1110 6 0.001480951323337365 1110 7 -0.0076011762425309826 1110 8 0.043799963587964896 1110 9 0.08768913296552228 1110 10 0.25022023296993723 1110 11 0.007074506047061391 1110 12 0.0823593460187837 1110 13 0.007007307046670519 1110 14 0.18198705469116305 1110 15 0.10567478981537448 1110 16 0.19855850866807145 1110 17 0.20111280481489757 1110 18 0.2307304601421336 1110 19 0.28255515929740227 1110 20 0.2618971827260168 1110 21 0.14168555880800682 1110 22 0.23594236932080292 1110 23 0.08814765950420878 1110 24 0.1823335701826636 1110 25 0.22920084921326378 1111 1 0.0059596178632368435 1111 2 0.018943019428288466 1111 3 0.03196046368477923 1111 4 0.12156461544489616 1111 5 0.002740269739234691 1111 6 -0.021946691062368018 1111 7 0.08182026635179085 1111 8 -0.07346266555990866 1111 9 0.03953820221003923 1111 10 0.10975545931192698 1111 11 0.019048563256575335 1111 12 -0.0042093017487374595 1111 13 0.08445655935652496 1111 14 0.08128471748769073 1111 15 0.03445026567457177 1111 16 0.011737452633916234 1111 17 -0.025675516022040767 1111 18 0.016731776057589798 1111 19 0.049157790434883 1111 20 0.07268112855745557 1111 21 0.0794088063721361 1111 22 0.13756048660156495 1111 23 0.08637634768798819 1111 24 0.1685045895850834 1111 25 0.1280197790864932 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25 -0.8345978609879978 2215 1 -0.7639732946631774 2215 2 -0.7665313457710248 2215 3 -0.8053721766620789 2215 4 -0.657017999256901 2215 5 -0.8459149033820097 2215 6 -0.8321747611178267 2215 7 -0.9142881130856608 2215 8 -1.0299057301102366 2215 9 -1.205005828646101 2215 10 -1.1835498810150966 2215 11 -1.1338226575166033 2215 12 -1.1065378730796098 2215 13 -1.0522693530946654 2215 14 -1.1943593154200514 2215 15 -1.1850792329816286 2215 16 -1.229100285950615 2215 17 -1.2859906425034708 2215 18 -1.2078731190712162 2215 19 -1.342404369768083 2215 20 -1.2003466980409172 2215 21 -1.1869673074782483 2215 22 -1.2452852258865477 2215 23 -1.1903767827188432 2215 24 -1.2169072700578027 2215 25 -1.2653800718302293 2216 1 -1.6760169802249365 2216 2 -1.5251942904712976 2216 3 -1.5449406187338823 2216 4 -1.5002736331458095 2216 5 -1.923960055996215 2216 6 -2.0243773773613576 2216 7 -2.1474646891482507 2216 8 -1.976140295495945 2216 9 -2.238632708307044 2216 10 -2.010952383154546 2216 11 -2.0399088875993314 2216 12 -2.4184841626594604 2216 13 -2.250091595239823 2216 14 -2.5781076344224516 2216 15 -2.617402066022912 2216 16 -2.5392143900298336 2216 17 -2.798900704038175 2216 18 -2.6478217722355666 2216 19 -2.6885382790553436 2216 20 -2.7341284332379487 2216 21 -2.916768440368749 2216 22 -2.8149025697065895 2216 23 -2.892332445133775 2216 24 -2.947302151674819 2216 25 -3.0348996121538008 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47223.0 3 0.0 4 727.0 5 6193.0 6 6971.0 7 5411.0 8 4313.0 9 3815.0 10 3511.0 11 3148.0 12 2807.0 13 2864.0 14 2693.0 15 2621.0 16 2669.0 17 2765.0 18 3075.0 19 3716.0 20 4863.0 21 6231.0 22 7755.0 23 9350.0 24 10763.0 25 11999.0 26 13335.0 27 14402.0 28 16527.0 29 19999.0 30 24821.0 31 31819.0 32 40394.0 33 52544.0 34 69958.0 35 93886.0 36 134584.0 37 222446.0 38 807071.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.49567729296486 19.38775570433829 13.607658631572672 27.508908371124186 2 14.284754694924203 20.731967437212457 38.30405290745909 26.67922496040425 3 16.987226510690924 24.75896881133311 29.97748627581993 28.276318402156036 4 11.740722279373902 17.339701326508127 36.51385368463351 34.40572270948447 5 13.166953223946281 37.8239099620518 35.428326481902076 13.58081033209984 6 32.367443586979135 36.88970616050994 17.510674970209386 13.232175282301537 7 27.83439051255561 31.434202159226928 22.377919520795185 18.353487807422276 8 25.861303945849528 35.60645567688381 20.302124452505495 18.230115924761158 9 26.46694901472782 14.550764908708247 19.512478228796205 39.46980784776773 10 15.009810217945924 28.618733097259618 34.18121505293982 22.190241631854633 11 34.56594093216809 22.20184307849846 23.975987306667356 19.256228682666094 12 23.013558840702327 24.692609126779548 31.123233853914726 21.17059817860339 13 28.975725120767535 21.275708211249956 25.665760701456282 24.082805966526223 14 21.620575413507385 21.185871961038128 27.05613613559354 30.137416489860946 15 23.94912061670837 29.37625090660349 23.074244565828987 23.60038391085915 16 23.171577398750582 27.368378259427352 26.321166532824204 23.138877808997865 17 22.497052027515586 27.819549315263043 26.993056279903282 22.690342377318085 18 22.626346445007993 26.90626909864137 28.04135826443334 22.42602619191729 19 23.676329444537078 26.664600602497305 27.184612456835065 22.474457496130547 20 24.097830102358554 26.448488719230717 27.116385204702375 22.33729597370835 21 23.719333679381073 26.102231772249525 26.8437205591833 23.334713989186106 22 23.744158259037466 26.71080819293123 26.373560333429957 23.17147321460135 23 23.14306454596984 26.565333748139558 26.861708082475733 23.42989362341487 24 23.425691507946 26.579817341859187 26.972565927970173 23.021925222224645 25 23.385256052969893 26.31437853583351 26.86084561877749 23.4395197924191 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 229.0 1 229.0 2 358.5 3 488.0 4 488.0 5 488.0 6 1042.0 7 1596.0 8 1596.0 9 1596.0 10 2118.5 11 2641.0 12 2641.0 13 2641.0 14 4567.0 15 6493.0 16 6493.0 17 6493.0 18 12091.5 19 17690.0 20 17690.0 21 17690.0 22 27578.5 23 37467.0 24 37467.0 25 37467.0 26 53214.5 27 68962.0 28 68962.0 29 68962.0 30 88221.5 31 107481.0 32 107481.0 33 107481.0 34 125381.5 35 143282.0 36 143282.0 37 143282.0 38 159410.5 39 175539.0 40 175539.0 41 175539.0 42 188616.0 43 201693.0 44 201693.0 45 201693.0 46 214485.5 47 227278.0 48 227278.0 49 227278.0 50 222821.5 51 218365.0 52 218365.0 53 218365.0 54 198586.0 55 178807.0 56 178807.0 57 178807.0 58 155618.5 59 132430.0 60 132430.0 61 132430.0 62 109871.5 63 87313.0 64 87313.0 65 87313.0 66 68483.0 67 49653.0 68 49653.0 69 49653.0 70 36852.5 71 24052.0 72 24052.0 73 24052.0 74 16936.0 75 9820.0 76 9820.0 77 9820.0 78 6886.0 79 3952.0 80 3952.0 81 3952.0 82 2720.5 83 1489.0 84 1489.0 85 1489.0 86 957.0 87 425.0 88 425.0 89 425.0 90 272.0 91 119.0 92 119.0 93 119.0 94 62.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18123232086369337 2 0.0068934270289506255 3 0.0048312907382389 4 0.0018264635717732426 5 0.07500284280217219 6 0.32175217953076385 7 0.026984526318456294 8 0.012961999541616562 9 0.0043010271206273135 10 0.0034761726043426233 11 0.0 12 0.0 13 0.0 14 0.0011783635946924147 15 0.0 16 0.0 17 0.02003218110977105 18 0.028634235351025678 19 0.0 20 0.0 21 4.7134543787696594E-4 22 5.891817973462074E-5 23 0.005361554355850487 24 0.0 25 1.7675453920386222E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1697269.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.94858459172126 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84931905666902 55.74446114161805 2 12.465386426041809 17.18941500928875 3 3.4427772681839905 7.1212385911751666 4 1.3223651813956043 3.6470083028240654 5 0.6077875022413247 2.095304400603847 6 0.3490497629679254 1.443989226522855 7 0.2094839521166985 1.011053539518839 8 0.14882031074679747 0.8208759827593446 9 0.10538579457194269 0.6539581234628414 >10 0.4606286770880199 5.467583321290905 >50 0.02428372279998812 1.1546641888987461 >100 0.012146622096107191 1.6062753297314911 >500 0.0015394338484504473 0.7197592370961801 >1k 0.001026289232300298 1.3244136052089301 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3378 0.19902561114354886 No Hit GTACATGGGAAGCAGTGGTATCAAC 3131 0.18447282074909752 No Hit CCCATGTACTCTGCGTTGATACCAC 2952 0.1739264665766004 No Hit TATCAACGCAGAGTACTTTTTTTTT 2569 0.15136080373824068 No Hit GGTATCAACGCAGAGTACTTTTTTT 2175 0.1281470409228001 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 5.8918179734620735E-5 0.0 0.0 0.0 0.0 9 5.8918179734620735E-5 0.0 0.0 0.0 0.0 10 5.8918179734620735E-5 0.0 0.0 0.0 0.0 11 5.8918179734620735E-5 0.0 0.0 0.0 0.0 12 5.8918179734620735E-5 0.0 0.0 0.0 0.0 13 5.8918179734620735E-5 0.0 0.0 0.0 5.8918179734620735E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACG 820 0.0 11.261162 4 AGGACGT 830 0.0 11.24018 5 GGACGTG 825 0.0 11.191258 6 CGTGAAA 580 0.0 11.124253 9 TTAGGAC 665 0.0 11.022862 3 GACGTGA 535 0.0 10.998742 7 TAGGACC 340 0.0 10.919707 4 ACGTGAA 640 0.0 10.823587 8 ACACCGT 80 3.704442E-4 10.70813 6 GTACTAG 90 9.269735E-5 10.582811 1 GGCGAGG 480 0.0 10.477011 19 GTAGGAC 805 0.0 10.170162 3 TGTAGGA 820 0.0 9.985008 2 TTAGACT 125 2.5022164E-6 9.900534 4 ACAATAC 165 9.8752935E-9 9.808222 3 TGGCGAG 855 0.0 9.769224 18 TACACCG 80 0.004439113 9.519745 5 TTTAGGA 740 0.0 9.3919325 2 CTTTAGG 700 0.0 9.388466 1 GTCCTAC 630 0.0 9.373347 1 >>END_MODULE