FastQCFastQC Report
Thu 26 May 2016
SRR1513201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522760
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT35970.14258193407220662No Hit
GTCCTAAAGTGTGTATTTCTCATTT31110.1233173191266708No Hit
CTGTAGGACGTGGAATATGGCAAGA28830.11427959853493792No Hit
GTACATGGGAAGCAGTGGTATCAAC26330.10436981718435363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7950.013.1856164
TCCAACG1406.9485395E-1011.52209918
GTCCTAC19800.011.33793451
GGCGAGG8500.011.27513419
AGGACGT16550.011.1471215
GTATTAC2400.011.1449481
GGACGTG16450.011.129876
TTAGGAC13050.011.0263713
GACGTGG7600.010.8602267
GTAGGAC18450.010.7431883
TGTAGGA20450.010.7251532
TGGCGAG18500.010.51460118
GTCCTAG3550.010.4946391
AGTGCGG1002.430949E-510.43787210
TCCTACA21700.010.414962
GACGTGA9800.010.3583577
CTGTGCG1106.133272E-610.3516089
CTGTAGG20950.010.3051821
ACACCGT1201.5530422E-610.276876
CCTACAG21900.010.2691393
Produced by FastQC (version 0.11.5)