FastQCFastQC Report
Thu 26 May 2016
SRR1513201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522760
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT37030.14678368136485437No Hit
CTGTAGGACGTGGAATATGGCAAGA33450.13259287447081766No Hit
CTTTAGGACGTGAAATATGGCGAGG31030.1230002061234521No Hit
GTATCAACGCAGAGTACTTTTTTTT30980.12280201049644042No Hit
GTCCTAAAGTGTGTATTTCTCATTT29430.11665794605907814No Hit
CCCATGTACTCTGCGTTGATACCAC29020.11503274191758232No Hit
GTACATGGGAAGCAGTGGTATCAAC28340.1123372813902234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC500.001513152813.2845988
GCACCGC751.4469946E-512.6940196
TAGGACC8000.012.4966694
CCGTTGA550.003007588712.123771
TCCAACG1302.6611815E-911.68038818
TGTAGGA20850.011.1435072
AGGACGT18950.011.1039635
GGACGTG18950.011.00266
AATCCCG2000.010.91169919
GGCGAGG8200.010.76127219
GTCCTAC18900.010.6850461
GTAGGAC20250.010.6734413
GCACCGT1252.1761298E-710.6629766
GACGTGG9550.010.6341827
CTGTAGG21250.010.5344351
GTACTAG2100.010.4330391
GACGTGA10700.010.2895867
ACGTGGA10150.010.2836458
GTCCTAA12050.010.2768171
TAGGACG21100.010.2432634
Produced by FastQC (version 0.11.5)