##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513201_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2522760 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.91724817263632 31.0 31.0 34.0 28.0 34.0 2 31.00742559736162 33.0 31.0 34.0 27.0 34.0 3 31.113671534351266 33.0 31.0 34.0 27.0 34.0 4 34.51220131919009 37.0 35.0 37.0 31.0 37.0 5 34.28988250963231 37.0 35.0 37.0 30.0 37.0 6 34.21457768475796 37.0 35.0 37.0 30.0 37.0 7 34.08449991279392 37.0 35.0 37.0 30.0 37.0 8 34.05884983113732 37.0 35.0 37.0 30.0 37.0 9 35.631040209928805 39.0 37.0 39.0 30.0 39.0 10 35.47672192360748 39.0 35.0 39.0 30.0 39.0 11 35.49961748243987 39.0 35.0 39.0 30.0 39.0 12 35.44189776276776 39.0 35.0 39.0 30.0 39.0 13 35.41785623681999 39.0 35.0 39.0 30.0 39.0 14 36.58863427357339 40.0 37.0 41.0 30.0 41.0 15 36.57348538901838 40.0 37.0 41.0 30.0 41.0 16 36.535835751319986 40.0 37.0 41.0 29.0 41.0 17 36.470388780541946 40.0 37.0 41.0 29.0 41.0 18 36.427552363284654 40.0 37.0 41.0 29.0 41.0 19 36.505647386196074 40.0 37.0 41.0 29.0 41.0 20 36.47639450443165 40.0 37.0 41.0 29.0 41.0 21 36.42209960519431 40.0 37.0 41.0 29.0 41.0 22 36.36815154830424 40.0 37.0 41.0 29.0 41.0 23 36.293786963484436 40.0 37.0 41.0 29.0 41.0 24 36.23700193438932 40.0 37.0 41.0 29.0 41.0 25 36.187426865813634 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4638880924859947 1101 2 0.554430230141552 1101 3 0.4812902330708688 1101 4 0.6412997969661873 1101 5 0.5669364558872445 1101 6 0.5376087555722933 1101 7 0.6081937445697534 1101 8 0.5660931308179187 1101 9 0.5496444095567128 1101 10 0.6258509716474805 1101 11 0.663472200631773 1101 12 0.673227844138097 1101 13 0.653249401911772 1101 14 0.7734954094202067 1101 15 0.8838103056298863 1101 16 0.739057343804987 1101 17 0.7257918494093261 1101 18 0.7788273656627069 1101 19 0.8034401146113481 1101 20 0.834008420741668 1101 21 0.8691107596178824 1101 22 0.8475377876395527 1101 23 0.7663707869997154 1101 24 0.9155510653621803 1101 25 0.7433730238304008 1102 1 0.2588816478369793 1102 2 0.25726305583905074 1102 3 0.2762138271254422 1102 4 0.42152994821465484 1102 5 0.37643477271125647 1102 6 0.30760892333662326 1102 7 0.3583865843166194 1102 8 0.3741713047138546 1102 9 0.385340519101824 1102 10 0.37116615264199737 1102 11 0.3938165208631901 1102 12 0.4061143294360505 1102 13 0.49881933483102614 1102 14 0.5661321257510608 1102 15 0.4313236991132783 1102 16 0.4164147628613719 1102 17 0.530645806987792 1102 18 0.49353269475029293 1102 19 0.4739731661481912 1102 20 0.47170235363378765 1102 21 0.39402222823756006 1102 22 0.3307447563557844 1102 23 0.3668525204978579 1102 24 0.4412376765904682 1102 25 0.5199184992084867 1103 1 0.502306555763262 1103 2 0.552944712111298 1103 3 0.545810816377962 1103 4 0.6828976048792512 1103 5 0.5768412737321285 1103 6 0.5132632922077818 1103 7 0.6060783462146091 1103 8 0.5613156766847567 1103 9 0.6568710512253944 1103 10 0.5686967999648829 1103 11 0.5875690364740649 1103 12 0.5524331408198435 1103 13 0.6980753823928083 1103 14 0.7999269025747822 1103 15 0.6927011540187991 1103 16 0.7588951273615336 1103 17 0.8307680260458099 1103 18 0.7427632703850477 1103 19 0.818771654475583 1103 20 0.7867667501226876 1103 21 0.6550716520345077 1103 22 0.7224050815775982 1103 23 0.7293294150209846 1103 24 0.8307169133830925 1103 25 0.7447588036286348 1104 1 0.2999214436602031 1104 2 0.33056447413003553 1104 3 0.2788950511739614 1104 4 0.49448030335067017 1104 5 0.3843830532886372 1104 6 0.3938882396924299 1104 7 0.3694999099848886 1104 8 0.4610084814977924 1104 9 0.3467360506030559 1104 10 0.47092840163686844 1104 11 0.5696671332306096 1104 12 0.5849859289883454 1104 13 0.5667927596829614 1104 14 0.5262752962936474 1104 15 0.5265352295690775 1104 16 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-1.1127168919320383 2215 23 -1.1459182229003417 2215 24 -1.2011232343777323 2215 25 -1.2990258146874183 2216 1 -0.5050198504606733 2216 2 -0.4189670678939841 2216 3 -0.46878339122232404 2216 4 -0.31991856735005086 2216 5 -0.48057177778904503 2216 6 -0.5301444319357529 2216 7 -0.7759266413534078 2216 8 -0.7349002477051414 2216 9 -0.9111777336166398 2216 10 -0.8817010912455601 2216 11 -0.822376550894873 2216 12 -0.9420172509769529 2216 13 -1.0161031124598736 2216 14 -1.1212074090244855 2216 15 -1.0778865381013745 2216 16 -0.9946019876424828 2216 17 -1.236959909567659 2216 18 -1.0720512594310918 2216 19 -1.1229801749252104 2216 20 -1.003397115679597 2216 21 -1.3590535936656991 2216 22 -1.0524135481154815 2216 23 -0.9937047524669893 2216 24 -1.0806207853547534 2216 25 -1.2514123668493013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51399.0 3 0.0 4 810.0 5 6962.0 6 7876.0 7 6257.0 8 4996.0 9 4464.0 10 4064.0 11 3793.0 12 3511.0 13 3438.0 14 3325.0 15 3246.0 16 3349.0 17 3676.0 18 4032.0 19 4852.0 20 6280.0 21 8341.0 22 10440.0 23 12801.0 24 14705.0 25 16647.0 26 18704.0 27 20983.0 28 23363.0 29 28662.0 30 36151.0 31 46657.0 32 59743.0 33 78023.0 34 103894.0 35 139463.0 36 200189.0 37 325487.0 38 1252177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.73608930675587 19.526738372492478 13.391843359718314 27.34532896103334 2 13.69418961941886 20.511413707707757 40.171581229532016 25.622815443341366 3 16.96514827354782 24.55858060787259 30.029283267548056 28.446987851031537 4 11.10626178060281 17.25990363442126 37.902512600661595 33.731321984314334 5 12.53006102258653 38.29256565422544 36.31407232735591 12.86330099583212 6 31.991441401484426 37.79721499481098 17.773809717963232 12.437533885741358 7 27.891258963780086 31.590016475313792 22.391596995188255 18.127127565717867 8 24.942286718551465 37.33465536359863 20.451454041674083 17.271603876175824 9 26.06530726476228 14.857476069608333 20.00864970631829 39.0685669593111 10 13.975088924239678 29.528476568120944 35.16650922659597 21.329925281043412 11 34.29069749005058 22.47411565111227 24.610624871172842 18.624561987664304 12 22.681864307345922 24.909900267960488 32.10907894528215 20.299156479411437 13 28.58777687929093 21.791173159555406 26.04361889359273 23.577431067560926 14 20.465876991793795 21.940829087265126 27.920392464342143 29.672901456598932 15 22.890762498216237 30.95050658802264 23.304674245667446 22.854056668093676 16 22.078556818722355 28.494347460717627 27.5628280137627 21.864267706797317 17 21.524950986125454 28.795735545119527 27.890708475980723 21.788604992774292 18 21.468511227156117 27.8902938529189 29.365228528039143 21.275966391885838 19 23.002465553600025 27.3795763370277 27.711712568773883 21.906245540598395 20 23.393228051816266 27.447874550095925 27.97091280977976 21.187984588308044 21 22.760584350153444 27.056360282138346 27.282384904631247 22.900670463076963 22 22.785129749024378 28.131229548648207 27.158126730499 21.925513971828416 23 21.98865854939636 27.768199936177083 27.61859268494275 22.624548829483807 24 22.616737224309883 27.220187413784902 28.239745358258418 21.9233300036468 25 22.489650207947182 27.413986925410143 27.796782566367895 22.29958030027478 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 98.0 1 98.0 2 250.0 3 402.0 4 402.0 5 402.0 6 938.5 7 1475.0 8 1475.0 9 1475.0 10 2210.5 11 2946.0 12 2946.0 13 2946.0 14 5980.0 15 9014.0 16 9014.0 17 9014.0 18 18027.0 19 27040.0 20 27040.0 21 27040.0 22 44457.0 23 61874.0 24 61874.0 25 61874.0 26 90016.0 27 118158.0 28 118158.0 29 118158.0 30 154187.0 31 190216.0 32 190216.0 33 190216.0 34 219124.0 35 248032.0 36 248032.0 37 248032.0 38 270850.5 39 293669.0 40 293669.0 41 293669.0 42 307918.5 43 322168.0 44 322168.0 45 322168.0 46 330591.0 47 339014.0 48 339014.0 49 339014.0 50 324123.5 51 309233.0 52 309233.0 53 309233.0 54 271986.5 55 234740.0 56 234740.0 57 234740.0 58 200055.5 59 165371.0 60 165371.0 61 165371.0 62 133995.0 63 102619.0 64 102619.0 65 102619.0 66 78872.0 67 55125.0 68 55125.0 69 55125.0 70 40293.0 71 25461.0 72 25461.0 73 25461.0 74 17790.5 75 10120.0 76 10120.0 77 10120.0 78 7116.5 79 4113.0 80 4113.0 81 4113.0 82 2738.0 83 1363.0 84 1363.0 85 1363.0 86 883.0 87 403.0 88 403.0 89 403.0 90 246.0 91 89.0 92 89.0 93 89.0 94 51.0 95 13.0 96 13.0 97 13.0 98 8.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1897524933009878 2 0.008839524964721178 3 0.006302620938971603 4 0.0029729344051752844 5 0.08141876357640046 6 0.34870538616435964 7 0.031909495948881386 8 0.014943950276681096 9 0.0051927254277061635 10 0.004241386418050072 11 0.0 12 0.0 13 0.0 14 0.0013477302636794623 15 0.0 16 0.0 17 0.020414149582203618 18 0.02972934405175284 19 0.0 20 0.0 21 0.001030617260460765 22 1.981956270116856E-4 23 0.006144064437362254 24 0.0 25 1.585565016093485E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2522760.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.78355004894104 #Duplication Level Percentage of deduplicated Percentage of total 1 81.8488482321969 57.93552045291889 2 12.375204279122201 17.51921782914231 3 3.1691437371865474 6.729697330002961 4 1.1071326615304427 3.134671206330295 5 0.49699353575858907 1.7589483406184128 6 0.2688390631247998 1.1417629967882812 7 0.1550327103262972 0.7681635927423105 8 0.0983863753196327 0.5571309537256893 9 0.07571111844236063 0.48231915673735226 >10 0.3664303295473954 4.553521328225816 >50 0.02272591946323156 1.1147269823674515 >100 0.01230180135783878 1.6812413669903843 >500 0.00224154249913601 1.1013288199934337 >1k 0.0010086941245867618 1.5217496434165079 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3703 0.14678368136485437 No Hit CTGTAGGACGTGGAATATGGCAAGA 3345 0.13259287447081766 No Hit CTTTAGGACGTGAAATATGGCGAGG 3103 0.1230002061234521 No Hit GTATCAACGCAGAGTACTTTTTTTT 3098 0.12280201049644042 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2943 0.11665794605907814 No Hit CCCATGTACTCTGCGTTGATACCAC 2902 0.11503274191758232 No Hit GTACATGGGAAGCAGTGGTATCAAC 2834 0.1123372813902234 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.9639125402337126E-5 0.0 5 0.0 0.0 0.0 3.9639125402337126E-5 0.0 6 0.0 0.0 0.0 3.9639125402337126E-5 0.0 7 0.0 0.0 0.0 3.9639125402337126E-5 0.0 8 0.0 0.0 0.0 3.9639125402337126E-5 0.0 9 0.0 0.0 0.0 3.9639125402337126E-5 0.0 10 3.9639125402337126E-5 0.0 0.0 1.1891737620701137E-4 0.0 11 3.9639125402337126E-5 0.0 0.0 2.7747387781635983E-4 0.0 12 3.9639125402337126E-5 0.0 0.0 2.7747387781635983E-4 0.0 13 3.9639125402337126E-5 0.0 0.0 2.7747387781635983E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCC 50 0.0015131528 13.284598 8 GCACCGC 75 1.4469946E-5 12.694019 6 TAGGACC 800 0.0 12.496669 4 CCGTTGA 55 0.0030075887 12.12377 1 TCCAACG 130 2.6611815E-9 11.680388 18 TGTAGGA 2085 0.0 11.143507 2 AGGACGT 1895 0.0 11.103963 5 GGACGTG 1895 0.0 11.0026 6 AATCCCG 200 0.0 10.911699 19 GGCGAGG 820 0.0 10.761272 19 GTCCTAC 1890 0.0 10.685046 1 GTAGGAC 2025 0.0 10.673441 3 GCACCGT 125 2.1761298E-7 10.662976 6 GACGTGG 955 0.0 10.634182 7 CTGTAGG 2125 0.0 10.534435 1 GTACTAG 210 0.0 10.433039 1 GACGTGA 1070 0.0 10.289586 7 ACGTGGA 1015 0.0 10.283645 8 GTCCTAA 1205 0.0 10.276817 1 TAGGACG 2110 0.0 10.243263 4 >>END_MODULE