FastQCFastQC Report
Thu 26 May 2016
SRR1513200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304797
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC27160.11784118080681293No Hit
GTCCTAAAGTGTGTATTTCTCATTT26830.1164093844273487No Hit
GTCCTACAGTGGACATTTCTAAATT26650.11562840458400458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAG400.005316485714.23526618
TAGGACC6600.011.8375954
ACTGCGC700.001507258710.8437998
GGCGAGG6800.010.7462319
AGGACGT12950.010.5739015
GTAATAC2001.8189894E-1210.4803733
GTCCTAA9350.010.41896251
GGACGTG12600.010.3157746
TGTAGGA17550.010.213342
GTATTAG2105.456968E-1210.0055111
GTAGGAC16150.09.9110763
TAGAAAT6550.09.8914774
GTGTAGG3500.09.8235921
CGCAGAT1554.143476E-89.7943988
GTCCTAG2350.09.7539221
TTAGGAC9450.09.67884353
GACGTGG6700.09.629947
GTAGAAC4850.09.6258353
TAGGACG14550.09.5605614
GACGTGA7150.09.5546757
Produced by FastQC (version 0.11.5)