##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513200_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2304797 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.62389095438774 31.0 30.0 34.0 25.0 34.0 2 29.4713998673202 31.0 30.0 34.0 23.0 34.0 3 29.670377911807417 31.0 30.0 34.0 25.0 34.0 4 32.76056372860603 37.0 33.0 37.0 25.0 37.0 5 32.90696490840625 37.0 35.0 37.0 26.0 37.0 6 33.26783313237565 37.0 35.0 37.0 27.0 37.0 7 33.244055767167346 37.0 35.0 37.0 27.0 37.0 8 33.12382392028452 37.0 35.0 37.0 26.0 37.0 9 34.3793566201275 39.0 35.0 39.0 25.0 39.0 10 33.96345968864069 39.0 34.0 39.0 22.0 39.0 11 34.41546826032835 39.0 35.0 39.0 27.0 39.0 12 34.41030077703156 39.0 35.0 39.0 27.0 39.0 13 34.4480069177459 39.0 35.0 39.0 27.0 39.0 14 34.69217375760208 40.0 34.0 41.0 17.0 41.0 15 35.12535464077747 40.0 35.0 41.0 24.0 41.0 16 34.9694515395499 40.0 35.0 41.0 22.0 41.0 17 34.58817804778469 39.0 34.0 41.0 17.0 41.0 18 34.327792859848394 39.0 33.0 41.0 17.0 41.0 19 34.95611977974633 40.0 34.0 41.0 23.0 41.0 20 35.167073282375846 40.0 35.0 41.0 25.0 41.0 21 35.210320908956405 40.0 36.0 41.0 25.0 41.0 22 34.903137239418484 40.0 35.0 41.0 19.0 41.0 23 34.44607746365515 40.0 34.0 41.0 17.0 41.0 24 34.694862497651634 40.0 35.0 41.0 18.0 41.0 25 34.3377286589665 39.0 34.0 41.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9329291797780677 1101 2 1.098566285223228 1101 3 1.0699203386854954 1101 4 1.4309549822223104 1101 5 1.3203949864336906 1101 6 1.1265530823580505 1101 7 1.0396015644852241 1101 8 1.1182099241708485 1101 9 1.3986431676283004 1101 10 1.72148621959699 1101 11 1.2351013040109962 1101 12 1.216105675736479 1101 13 1.172172734798508 1101 14 2.186876302312868 1101 15 1.8244414883044513 1101 16 1.795856258274469 1101 17 2.131162861501217 1101 18 2.1270099887477443 1101 19 1.720711670217547 1101 20 1.2930022145083768 1101 21 1.4521867333291425 1101 22 1.7103917381317046 1101 23 1.9734101754751023 1101 24 1.6552629896805016 1101 25 1.9266938457801004 1102 1 1.0624475460765517 1102 2 1.2916722874494475 1102 3 1.1497739220402359 1102 4 1.5272654540092745 1102 5 1.5046363598389476 1102 6 1.1886461553951264 1102 7 1.1187461830313339 1102 8 1.2608753921047509 1102 9 1.5487026761478688 1102 10 1.7796825548996722 1102 11 1.4494364354434808 1102 12 1.3831259685697717 1102 13 1.363617207784209 1102 14 2.307876816055483 1102 15 1.8742291413569347 1102 16 1.9988667899980754 1102 17 2.3354663121391113 1102 18 2.5510124718370477 1102 19 2.0030376399067364 1102 20 1.856661346283353 1102 21 1.5976285519779978 1102 22 1.8378525652573146 1102 23 2.113041137030031 1102 24 1.7772755763745636 1102 25 2.0847921418670907 1103 1 0.9358519048091054 1103 2 1.2579007579542107 1103 3 1.0693382255280035 1103 4 1.503294318650724 1103 5 1.3306892741401057 1103 6 1.0314974729503632 1103 7 1.090548114831293 1103 8 1.113652843749783 1103 9 1.5208982396484387 1103 10 1.800991942094143 1103 11 1.3657602839950087 1103 12 1.332251280552562 1103 13 1.3258512886137837 1103 14 2.2241006786201964 1103 15 1.833991851381164 1103 16 1.908804917356484 1103 17 2.305814154802576 1103 18 2.4665339075954265 1103 19 1.947801189459767 1103 20 1.6429958372827187 1103 21 1.5339607074461128 1103 22 1.8766069822519 1103 23 2.2262025500138165 1103 24 1.9136009146163033 1103 25 2.1688364235397373 1104 1 0.8864317916993194 1104 2 1.1009729111654778 1104 3 0.960435916626249 1104 4 1.3015621050251482 1104 5 1.3085725119998912 1104 6 1.0195363728683517 1104 7 0.8801744855830833 1104 8 0.9882722019023049 1104 9 1.2477931446570096 1104 10 1.4277348370653868 1104 11 1.0020399961786381 1104 12 0.8681387203688544 1104 13 0.8342816697812339 1104 14 1.8526748525689527 1104 15 1.4099299260862779 1104 16 1.591727550962787 1104 17 1.9326306421338586 1104 18 1.9460485124592282 1104 19 1.3949793113208528 1104 20 1.1896147993745458 1104 21 1.1708013941196995 1104 22 1.4362415058663913 1104 23 1.7895351733481277 1104 24 1.4711103752068837 1104 25 1.6253330943635405 1105 1 0.5251362851610857 1105 2 0.7746756156775199 1105 3 0.6720494231842231 1105 4 1.0339612745011948 1105 5 0.8986293527354192 1105 6 0.6110481818030493 1105 7 0.5395480885493953 1105 8 0.5929107457096734 1105 9 0.8841733722538905 1105 10 1.2001595271764032 1105 11 0.7289190134865251 1105 12 0.725173473776259 1105 13 0.5724777331959103 1105 14 1.4467181518717354 1105 15 1.0527326200711826 1105 16 1.1990000360035111 1105 17 1.6159190146077123 1105 18 1.161530223569521 1105 19 0.8524393478099483 1105 20 0.8356357778275907 1105 21 0.7941225790755269 1105 22 1.026250895674849 1105 23 1.451848576932619 1105 24 1.2731240365942469 1105 25 1.4394054299836156 1106 1 0.6765315994693495 1106 2 0.8767619464664484 1106 3 0.7030075310092343 1106 4 1.2090567761313622 1106 5 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1107 18 1.7528738657076985 1107 19 1.3034735797351757 1107 20 1.046981208482876 1107 21 1.0080878951521086 1107 22 1.2059446859661875 1107 23 1.5167987703018255 1107 24 1.3974343336000743 1107 25 1.652541244162201 1108 1 0.6057084263081407 1108 2 0.7972906738550876 1108 3 0.6949005105295143 1108 4 1.1097851210193994 1108 5 1.0721530829307184 1108 6 0.6672140208907678 1108 7 0.704231438906902 1108 8 0.716395647898544 1108 9 0.9532796514353095 1108 10 1.178706973066923 1108 11 0.7130193476329367 1108 12 0.4921036929208853 1108 13 0.5126453058493965 1108 14 1.5381353902176897 1108 15 0.9786814089918252 1108 16 1.1056844142559612 1108 17 1.4799202970597065 1108 18 1.7789101870293038 1108 19 1.1647433952671165 1108 20 0.9907715469604526 1108 21 0.8714068258605394 1108 22 1.12810819550117 1108 23 1.4476170492358449 1108 24 1.0871499691914082 1108 25 1.3708015787609327 1109 1 0.2891000746763126 1109 2 0.500993104292089 1109 3 0.4449774895079095 1109 4 0.6744258492421054 1109 5 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1.2012877373580082 1110 18 0.8331231259483758 1110 19 0.530724776484135 1110 20 0.4166036700366149 1110 21 0.31863119043664767 1110 22 0.6168355439961459 1110 23 -0.5938770715576709 1110 24 0.11872524200462209 1110 25 0.4839783250298666 1111 1 0.33700017369287494 1111 2 0.709063242626879 1111 3 0.6192550242838735 1111 4 0.9615635014920869 1111 5 0.8498574610787273 1111 6 0.48115551643513044 1111 7 0.42150420140205824 1111 8 0.46140262193961235 1111 9 0.6964342426790111 1111 10 0.8958979843028771 1111 11 0.6150363952888824 1111 12 0.41950121326183165 1111 13 0.2854546820601698 1111 14 1.256261697850391 1111 15 0.9949607580813264 1111 16 0.9709637633454591 1111 17 1.2942054578500972 1111 18 1.554642848788312 1111 19 1.0362796215360035 1111 20 0.6203957236361646 1111 21 0.4483510335319849 1111 22 0.8224018801195356 1111 23 1.2183148407895033 1111 24 0.9070500079360784 1111 25 1.076134008051909 1112 1 0.022387661157196703 1112 2 0.17183382042522766 1112 3 0.1316435917451102 1112 4 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-1.9897134962175116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96658.0 3 2.0 4 829.0 5 4895.0 6 5803.0 7 5429.0 8 5091.0 9 4690.0 10 4423.0 11 4311.0 12 4220.0 13 4188.0 14 4184.0 15 4236.0 16 4221.0 17 4689.0 18 5342.0 19 6555.0 20 8275.0 21 10603.0 22 13301.0 23 15690.0 24 18223.0 25 20958.0 26 23664.0 27 26479.0 28 29521.0 29 35749.0 30 42459.0 31 51212.0 32 65948.0 33 85634.0 34 112853.0 35 151910.0 36 216960.0 37 316983.0 38 888609.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.505345966620304 18.48404902642559 12.220184283727399 25.790420723226703 2 14.392039260823305 20.365638601720313 41.251530091583646 23.990792045872734 3 18.334390424063038 25.039216761021805 29.789469288251674 26.83692352666348 4 11.422060875292082 16.85322344927444 39.10544275653937 32.61927291889411 5 12.689508291993715 37.98770164482671 36.29454630240242 13.028243760777155 6 31.597381491213415 37.25165067166009 18.346963263198322 12.804004573928177 7 27.83154541825062 31.477026159107087 22.717457341734796 17.973971080907496 8 24.989639309090546 36.49684101991726 21.109468347926537 17.40405132306565 9 26.700174060815833 14.804195685843444 20.161719031734094 38.33391122160663 10 13.900571149342033 28.701632295433626 35.744896174588305 21.652900380636037 11 34.24654324084352 22.236306842000655 24.853992013282845 18.663157903872975 12 23.060494390292913 24.628417394670702 32.040365760453724 20.27072245458266 13 29.015850089685962 21.712118693609924 25.71398315474984 23.558048061954274 14 20.471143430499815 21.551810935047982 28.711922104795367 29.26512352965684 15 23.21788300209348 30.332634638398243 23.374535933595478 23.0749464259128 16 22.186004971566252 27.684932196446304 27.85821662419542 22.270846207792026 17 21.648383272832394 28.06372543700227 28.30866634169929 21.979224948466047 18 21.499000024304966 27.239094883110482 29.78573437451173 21.476170718072822 19 23.568634871476164 26.845354757734256 27.4112432969181 22.17476707387148 20 23.8222114201516 27.012979121863996 27.568557583596093 21.596251874388315 21 23.334094337952024 26.555357443580043 26.904146404557 23.206401813910933 22 23.27732482201899 27.566492472947452 27.066005659451065 22.0901770455825 23 22.381633152612636 27.2916026505702 27.74231937044618 22.584444826370987 24 23.23504923899426 26.95621145810453 27.657529620230747 22.151209682670462 25 22.870351441221956 26.941164879055762 27.592838792089807 22.595644887632478 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 211.0 1 211.0 2 339.0 3 467.0 4 467.0 5 467.0 6 923.0 7 1379.0 8 1379.0 9 1379.0 10 2276.5 11 3174.0 12 3174.0 13 3174.0 14 6065.5 15 8957.0 16 8957.0 17 8957.0 18 17348.0 19 25739.0 20 25739.0 21 25739.0 22 41219.5 23 56700.0 24 56700.0 25 56700.0 26 81490.5 27 106281.0 28 106281.0 29 106281.0 30 137043.5 31 167806.0 32 167806.0 33 167806.0 34 193932.5 35 220059.0 36 220059.0 37 220059.0 38 240499.0 39 260939.0 40 260939.0 41 260939.0 42 274512.5 43 288086.0 44 288086.0 45 288086.0 46 297380.0 47 306674.0 48 306674.0 49 306674.0 50 294186.0 51 281698.0 52 281698.0 53 281698.0 54 250830.5 55 219963.0 56 219963.0 57 219963.0 58 189208.0 59 158453.0 60 158453.0 61 158453.0 62 129598.5 63 100744.0 64 100744.0 65 100744.0 66 77785.5 67 54827.0 68 54827.0 69 54827.0 70 40397.0 71 25967.0 72 25967.0 73 25967.0 74 18204.0 75 10441.0 76 10441.0 77 10441.0 78 7303.0 79 4165.0 80 4165.0 81 4165.0 82 2807.0 83 1449.0 84 1449.0 85 1449.0 86 935.0 87 421.0 88 421.0 89 421.0 90 284.5 91 148.0 92 148.0 93 148.0 94 94.0 95 40.0 96 40.0 97 40.0 98 24.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17342091299146953 2 0.25663865407669306 3 0.2618451863656539 4 0.45557157528407055 5 0.2582873893015307 6 0.0187435162402589 7 0.01896045508563227 8 0.017962536396914783 9 0.018656740702109556 10 0.021520333461038002 11 0.020435639234171167 12 0.02889625420373248 13 0.02802849882223901 14 0.024861191679787852 15 0.04243323815503058 16 0.02139017015381398 17 0.04234646261688123 18 0.032150336884332976 19 0.027464457824268252 20 0.03089209158116745 21 0.03579490948660554 22 0.045123279837660325 23 0.029894172892449965 24 0.049245117899754304 25 0.038354787862011276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2304797.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.53326597726146 #Duplication Level Percentage of deduplicated Percentage of total 1 83.20654786309584 60.35242667203667 2 11.397355706091547 16.53374865734794 3 2.8482854643535505 6.197863414953711 4 1.0466659569325543 3.036724009741355 5 0.46884855891028654 1.7003558613247778 6 0.2719275587688018 1.1834276368034942 7 0.16636737484032144 0.8447018334462255 8 0.11305487580732139 0.6560191501566964 9 0.08356381352966623 0.5455040681539405 >10 0.36668379015332553 4.543803474388278 >50 0.017364799005254224 0.8830228898190826 >100 0.010670978251138203 1.5040312730660876 >500 0.0018763879107887163 0.9001514327509217 >1k 7.868723496825237E-4 1.118219626010636 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2716 0.11784118080681293 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2683 0.1164093844273487 No Hit GTCCTACAGTGGACATTTCTAAATT 2665 0.11562840458400458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.338776907467339E-5 7 0.0 0.0 0.0 0.0 4.338776907467339E-5 8 0.0 0.0 0.0 0.0 4.338776907467339E-5 9 0.0 0.0 0.0 0.0 4.338776907467339E-5 10 0.0 0.0 0.0 0.0 4.338776907467339E-5 11 0.0 0.0 0.0 0.0 4.338776907467339E-5 12 0.0 0.0 0.0 0.0 1.7355107629869355E-4 13 0.0 0.0 0.0 0.0 1.7355107629869355E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCGAG 40 0.0053164857 14.235266 18 TAGGACC 660 0.0 11.837595 4 ACTGCGC 70 0.0015072587 10.843799 8 GGCGAGG 680 0.0 10.74623 19 AGGACGT 1295 0.0 10.573901 5 GTAATAC 200 1.8189894E-12 10.480373 3 GTCCTAA 935 0.0 10.4189625 1 GGACGTG 1260 0.0 10.315774 6 TGTAGGA 1755 0.0 10.21334 2 GTATTAG 210 5.456968E-12 10.005511 1 GTAGGAC 1615 0.0 9.911076 3 TAGAAAT 655 0.0 9.891477 4 GTGTAGG 350 0.0 9.823592 1 CGCAGAT 155 4.143476E-8 9.794398 8 GTCCTAG 235 0.0 9.753922 1 TTAGGAC 945 0.0 9.6788435 3 GACGTGG 670 0.0 9.62994 7 GTAGAAC 485 0.0 9.625835 3 TAGGACG 1455 0.0 9.560561 4 GACGTGA 715 0.0 9.554675 7 >>END_MODULE