FastQCFastQC Report
Thu 26 May 2016
SRR1513200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304797
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31120.1350227373603836No Hit
GTACATGGGAAGCAGTGGTATCAAC30160.13085751152921493No Hit
GTCCTACAGTGGACATTTCTAAATT28470.12352497855559513No Hit
CCCATGTACTCTGCGTTGATACCAC28320.12287416201947504No Hit
GTCCTAAAGTGTGTATTTCTCATTT26910.11675648657994608No Hit
CTTTAGGACGTGAAATATGGCGAGG26560.11523791466233253No Hit
CTGTAGGACGTGGAATATGGCAAGA24710.10721117738351794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7250.012.34448054
TATGTCG701.1012098E-412.20251216
ATGTCGA855.3777814E-511.16569617
TTATACT2850.010.3561854
GGTGCGT856.473265E-410.0793126
GTCCTAC16400.09.9906691
AGGACGT15950.09.7896275
TGTAGGA18550.09.7532512
GTAGGAC17500.09.5762483
GGACGTG16150.09.5488226
GTCCTAA8200.09.5259871
ATAGGAC2900.09.5218383
TCTACGC900.00109078939.5218383
TCCTACA17250.09.4946522
AGGACCT13450.09.4856155
TTAGGAC10500.09.4311543
TGGCGAG18650.09.41328118
GGCGAGG8300.09.37344419
TTAGACA2750.09.3478994
CCTACAG17750.09.33408453
Produced by FastQC (version 0.11.5)