FastQCFastQC Report
Thu 26 May 2016
SRR1513199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63800
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24143.7836990595611284No Hit
CCCATGTACTCTGCGTTGATACCAC22643.54858934169279No Hit
GAGTACATGGGAAGCAGTGGTATCA10221.6018808777429467No Hit
CATGTACTCTGCGTTGATACCACTG8161.2789968652037618No Hit
TATCAACGCAGAGTACATGGGAAGC7041.103448275862069No Hit
GCGTTGATACCACTGCTTCCCATGT6771.061128526645768No Hit
GTATCAACGCAGAGTACATGGGAAG6310.9890282131661442No Hit
ACGCAGAGTACATGGGAAGCAGTGG5950.9326018808777429No Hit
GGTATCAACGCAGAGTACATGGGAA5760.9028213166144201No Hit
GTATCAACGCAGAGTACTTTTTTTT5680.890282131661442No Hit
ACTCTGCGTTGATACCACTGCTTCC5680.890282131661442No Hit
TATCAACGCAGAGTACTTTTTTTTT4060.6363636363636364No Hit
GGTATCAACGCAGAGTACTTTTTTT3870.6065830721003135No Hit
GTACTCTGCGTTGATACCACTGCTT3100.48589341692789967No Hit
GCTTCCCATGTACTCTGCGTTGATA3040.4764890282131662No Hit
GTGGTATCAACGCAGAGTACATGGG3030.47492163009404387No Hit
ATACCACTGCTTCCCATGTACTCTG2740.4294670846394984No Hit
GGGAAGCAGTGGTATCAACGCAGAG2670.41849529780564265No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2660.41692789968652033No Hit
CTGCTTCCCATGTACTCTGCGTTGA2620.4106583072100313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2560.4012539184952978No Hit
TACCACTGCTTCCCATGTACTCTGC2280.3573667711598746No Hit
GCAGTGGTATCAACGCAGAGTACAT2260.3542319749216301No Hit
GATACCACTGCTTCCCATGTACTCT2220.34796238244514105No Hit
GTTGATACCACTGCTTCCCATGTAC2140.335423197492163No Hit
GCAGAGTACATGGGAAGCAGTGGTA2080.32601880877742945No Hit
CCACTGCTTCCCATGTACTCTGCGT2070.32445141065830724No Hit
CAGTGGTATCAACGCAGAGTACATG2060.3228840125391849No Hit
CATGGGAAGCAGTGGTATCAACGCA2030.3181818181818182No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1950.3056426332288401No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1870.2931034482758621No Hit
ACATGGGAAGCAGTGGTATCAACGC1840.2884012539184953No Hit
AAGCAGTGGTATCAACGCAGAGTAC1820.2852664576802508No Hit
CTTCCCATGTACTCTGCGTTGATAC1620.25391849529780564No Hit
ATGGGAAGCAGTGGTATCAACGCAG1610.25235109717868337No Hit
GAACAAAAAAAAAAAAAAAAAAAAA1610.25235109717868337No Hit
GTACATGGGAAGCAGTTGTATCAAC1350.2115987460815047No Hit
TTCCCATGTACTCTGCGTTGATACC1310.20532915360501566No Hit
GTACATGGGAAGCAGTGTTATCAAC1230.19278996865203762No Hit
TGATACCACTGCTTCCCATGTACTC1220.19122257053291536No Hit
CCATGTACTCTGCGTTGATACCACT1220.19122257053291536No Hit
CCCATGTACTCTGCGTTTATACCAC1210.1896551724137931No Hit
ATCAACGCAGAGTACATGGGAAGCA1070.1677115987460815No Hit
GTACATGGTAAGCAGTGGTATCAAC1050.164576802507837No Hit
GCAGAGTACTTTTTTTTTTTTTTTT780.12225705329153606No Hit
GGAAGCAGTGGTATCAACGCAGAGT750.11755485893416928No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA740.11598746081504703No Hit
ACCATGTACTCTGCGTTGATACCAC730.11442006269592477No Hit
AAAAAGTACTCTGCGTTGATACCAC680.10658307210031348No Hit
ATTCCATTCCATTCCATTCCATTCC670.10501567398119123No Hit
GAGTACATGTGAAGCAGTGGTATCA650.10188087774294671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA504.206904E-617.1908511
AACAAAA801.8527771E-613.1009042
CATGGTA603.9251655E-412.693924
ACATGGT750.002542615910.1551363
TGGTAAG800.00445235159.4905966
ACAAAAA1252.5591053E-59.1396223
ATGGTAA850.00719195938.9533765
GGTAAGC850.0073326418.9323257
GGAAGCA6950.08.3298758
GAAGCAG6900.08.2526919
AAGCAGT7800.07.78715510
GTGGTAT7200.07.513388615
TACATGG12250.07.46678642
GTACATG12600.07.4281461
AGTGGTA7300.07.41046514
CAGTGGT7300.07.41046513
TGGTATC7450.07.388651416
AGCAGTG7800.07.30045811
TGGGAAG7850.07.2539586
GGGAAGC7750.07.22509867
Produced by FastQC (version 0.11.5)