##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513199_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	63800
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.26796238244514	31.0	26.0	33.0	2.0	34.0
2	26.021379310344827	31.0	26.0	34.0	2.0	34.0
3	26.26717868338558	31.0	26.0	34.0	2.0	34.0
4	28.799059561128526	35.0	27.0	37.0	2.0	37.0
5	29.213291536050157	35.0	29.0	37.0	2.0	37.0
6	29.172570532915362	35.0	29.0	37.0	2.0	37.0
7	29.057460815047023	35.0	29.0	37.0	2.0	37.0
8	28.676050156739812	35.0	28.0	37.0	2.0	37.0
9	29.385611285266457	37.0	26.0	39.0	2.0	39.0
10	29.082899686520378	37.0	25.0	39.0	2.0	39.0
11	29.456880877742947	37.0	27.0	39.0	2.0	39.0
12	29.456300940438872	37.0	27.0	39.0	2.0	39.0
13	29.402210031347963	37.0	27.0	39.0	2.0	39.0
14	29.594184952978058	38.0	23.0	41.0	2.0	41.0
15	29.840156739811913	38.0	26.0	41.0	2.0	41.0
16	29.656755485893417	38.0	25.0	40.0	2.0	41.0
17	29.458056426332288	38.0	24.0	40.0	2.0	41.0
18	29.26015673981191	37.0	23.0	40.0	2.0	41.0
19	29.717460815047023	38.0	26.0	40.0	2.0	41.0
20	29.969702194357367	38.0	27.0	40.0	2.0	41.0
21	29.99101880877743	38.0	27.0	41.0	2.0	41.0
22	29.802633228840126	38.0	27.0	41.0	2.0	41.0
23	29.418996865203763	38.0	25.0	41.0	2.0	41.0
24	29.617210031347962	38.0	27.0	41.0	2.0	41.0
25	29.303557993730408	38.0	25.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.1849562545030068
1101	2	0.9657100331465713
1101	3	1.0731732116452264
1101	4	0.9700108483103023
1101	5	1.2554049457246101
1101	6	0.7630816157939151
1101	7	0.5149740113923045
1101	8	0.4112724707110367
1101	9	0.29731054218536457
1101	10	1.6183406265116282
1101	11	1.4256521174482408
1101	12	1.483294676256076
1101	13	1.5420120691781705
1101	14	1.7804859944289824
1101	15	1.2828889671020605
1101	16	2.030273086700788
1101	17	1.7521425096169807
1101	18	2.4529667937160937
1101	19	1.8175206991501014
1101	20	1.8025776396547748
1101	21	1.5493254411801871
1101	22	1.357476566282461
1101	23	2.0725916163717812
1101	24	1.6327852561162857
1101	25	1.804927300539223
1102	1	1.2422661960234755
1102	2	0.906645705661191
1102	3	0.9497813987797308
1102	4	1.7267359945091343
1102	5	1.463592080227535
1102	6	1.0227307386009308
1102	7	1.8290090991116017
1102	8	0.7849566812373503
1102	9	1.355790074349109
1102	10	1.7107382873303436
1102	11	1.7876404215418091
1102	12	1.058733272747304
1102	13	0.8624799054354781
1102	14	2.249491842382202
1102	15	1.7255790255815917
1102	16	1.516822794303124
1102	17	2.2632536207280936
1102	18	2.3664170861137563
1102	19	1.670152278097472
1102	20	1.7417589262044793
1102	21	2.029442400244516
1102	22	1.5878859230076081
1102	23	2.583117932161251
1102	24	2.5199197590402633
1102	25	2.4300734993696302
1103	1	0.8450439738012534
1103	2	1.1830345945500795
1103	3	1.160892509890843
1103	4	1.1052082167313557
1103	5	0.7684531913386472
1103	6	0.8977307386009308
1103	7	1.4769257657782688
1103	8	1.0641233479040189
1103	9	1.5488456299046653
1103	10	1.598932731774788
1103	11	2.2056959770973634
1103	12	2.127483272747302
1103	13	2.029841016546591
1103	14	2.711297397937752
1103	15	1.8519679144704817
1103	16	2.822378349858681
1103	17	2.485475842950315
1103	18	2.640028197224865
1103	19	1.9743189447641356
1103	20	1.5618978150933671
1103	21	1.8322201780222933
1103	22	1.9038581452298295
1103	23	1.460201265494586
1103	24	2.4289475368180433
1103	25	1.7897957215918545
1104	1	0.7874198603260751
1104	2	1.4565275023514985
1104	3	0.9623109496071542
1104	4	2.1191709826888037
1104	5	1.7739939714804898
1104	6	1.8170569797356855
1104	7	1.5963849856364263
1104	8	1.5955949791096913
1104	9	1.7744661972805496
1104	10	2.1700763487960657
1104	11	1.7141179629129688
1104	12	1.8253999394139697
1104	13	2.001250591014678
1104	14	2.755402007866831
1104	15	2.4038296165981414
1104	16	2.4979102647522993
1104	17	2.875546764936125
1104	18	2.964053019919902
1104	19	2.5722799376719365
1104	20	2.38880384346216
1104	21	1.8029648588733558
1104	22	2.83138474097451
1104	23	1.9001392087569968
1104	24	2.5218110119953465
1104	25	2.9693169981875975
1105	1	0.744866668836714
1105	2	0.9246126087344742
1105	3	0.781459885777366
1105	4	0.746830557156887
1105	5	1.8378237587145314
1105	6	0.48726974569312986
1105	7	0.41553392180663806
1105	8	0.467935404641608
1105	9	0.9872321547273586
1105	10	1.372204008370531
1105	11	1.341777537381052
1105	12	1.219016960690567
1105	13	1.5969952718657403
1105	14	1.276678603611515
1105	15	1.637872169789631
1105	16	1.2106762221991083
1105	17	1.6734191053616598
1105	18	1.1236274880050097
1105	19	0.9658969589485338
1105	20	1.2611442689940766
1105	21	1.1859435822776128
1105	22	1.3952145282085517
1105	23	1.2192881449272122
1105	24	1.7664918630591764
1105	25	1.6395297641450455
1106	1	1.4492106404679177
1106	2	1.2767845945500795
1106	3	1.9213091765575072
1106	4	1.7718748833980236
1106	5	1.0913698580053115
1106	6	1.5331474052675986
1106	7	1.6540090991116045
1106	8	0.866206681237351
1106	9	1.2050956299046653
1106	10	1.0780993984414557
1106	11	1.4973626437640313
1106	12	0.7212332727473019
1106	13	0.998591016546591
1106	14	1.4821307312710879
1106	15	0.6540512478038139
1106	16	1.6244616831920133
1106	17	1.6417258429503114
1106	18	1.9629448638915328
1106	19	1.3805689447641392
1106	20	0.541064481760035
1106	21	0.15513684468896116
1106	22	0.7267748118964974
1106	23	1.3560345988279217
1106	24	1.1268642034847076
1106	25	0.9981290549251867
1107	1	0.9372156909729696
1107	2	0.9500800490955328
1107	3	0.8881652371635695
1107	4	0.9590592268323661
1107	5	1.316117332752789
1107	6	0.7298014456716402
1107	7	1.013857583960089
1107	8	1.347582943863614
1107	9	1.4547799733390079
1107	10	1.4319504085424661
1107	11	1.2997616336630209
1107	12	1.093076707090738
1107	13	0.9897526327082069
1107	14	1.9161585090488664
1107	15	0.8235588235613918
1107	16	0.9713682488485809
1107	17	1.6268899843644533
1107	18	1.774497894194564
1107	19	1.456010863956056
1107	20	1.5154962999418515
1107	21	1.2425737133758297
1107	22	1.294956630078314
1107	23	1.6871583362016587
1107	24	0.8219399610604654
1107	25	1.2108815801777126
1108	1	0.6019209964549752
1108	2	0.1492562773979742
1108	3	0.3971384645833993
1108	4	0.48293879925562777
1108	5	0.1585219615651816
1108	6	0.3690090557530361
1108	7	-0.011443975322052324
1108	8	0.04612172978103857
1108	9	0.35730032246129895
1108	10	0.2503283628427475
1108	11	0.7176296340552923
1108	12	-0.1697456916539899
1108	13	-0.29631189607476927
1108	14	0.7306137409798268
1108	15	0.6426232542763053
1108	16	0.6840288352955746
1108	17	0.8608803736946484
1108	18	1.381735316965969
1108	19	0.918695967417861
1108	20	0.9497416662260534
1108	21	0.40624930423588523
1108	22	0.2858363005696347
1108	23	0.7297198739088273
1108	24	0.4915486695041267
1108	25	0.4590918380967075
1109	1	0.3917611455184229
1109	2	0.3339184329339169
1109	3	1.2518016007999329
1109	4	0.5247157924889301
1109	5	1.2757132923487475
1109	6	0.6792963951665882
1109	7	1.1855747556772585
1109	8	1.731421327701998
1109	9	1.3335678521268868
1109	10	2.3612433378353934
1109	11	1.249256583157969
1109	12	1.2647938788079074
1109	13	1.4139950569506325
1109	14	1.47171406460442
1109	15	1.6518416518442187
1109	16	2.324903602383934
1109	17	2.616788974263443
1109	18	1.8553059750026435
1109	19	1.991364399309596
1109	20	1.858930643376194
1109	21	1.7981292689313868
1109	22	2.062633397755082
1109	23	1.9699866190299424
1109	24	1.983556122676628
1109	25	2.2815886508847854
1110	1	1.3533773071345863
1110	2	1.5657100331465692
1110	3	0.9573837379610168
1110	4	1.6331687430471469
1110	5	1.2448786299351369
1110	6	0.9209763526360213
1110	7	1.304447695602832
1110	8	0.7270619443952455
1110	9	1.0236263316590488
1110	10	1.323603784406366
1110	11	0.9309152753429792
1110	12	1.3464525709929163
1110	13	1.752538384967643
1110	14	1.864696520744772
1110	15	1.6513100197336392
1110	16	1.8618520340692086
1110	17	1.6574056675117177
1110	18	1.7477036358213596
1110	19	1.1754154359922069
1110	20	1.318367113338983
1110	21	1.075641230653872
1110	22	0.7048449873350933
1110	23	0.5462758268980963
1110	24	0.38015367716891646
1110	25	0.994400984749749
1111	1	1.0742106404679177
1111	2	1.1309512612167438
1111	3	1.1713091765575072
1111	4	1.1677082167313557
1111	5	1.4038698580053115
1111	6	0.8560640719342665
1111	7	0.5498424324449367
1111	8	-0.14420998542931684
1111	9	1.4863456299046653
1111	10	1.30726606510812
1111	11	1.0077793104306991
1111	12	1.4087332727473019
1111	13	1.061091016546591
1111	14	2.523797397937752
1111	15	2.1853012478038174
1111	16	2.259878349858681
1111	17	2.6000591762836436
1111	18	3.1191948638915363
1111	19	2.5993189447641356
1111	20	1.791064481760035
1111	21	1.9051368446889576
1111	22	2.0496914785631652
1111	23	2.251867932161254
1111	24	2.6581142034847076
1111	25	2.84187905492519
1112	1	-0.3093677909046306
1112	2	-0.17113207211658832
1112	3	0.7393238824398622
1112	4	0.6940562559470429
1112	5	0.3413698580053115
1112	6	0.14037779742445977
1112	7	0.13685223636650434
1112	8	0.8790743282961735
1112	9	0.3270319044144685
1112	10	0.8238101827551816
1112	11	0.6726077418032474
1112	12	-0.047149080193872095
1112	13	-0.244056042276938
1112	14	1.148184652839717
1112	15	0.9046639929018525
1112	16	0.35669207534887803
1112	17	1.2795934900091375
1112	18	1.6461556482052586
1112	19	1.4779954153523747
1112	20	1.148294873916896
1112	21	1.128788805473274
1112	22	1.035598341308262
1112	23	1.1066473439259603
1112	24	0.8192661642690204
1112	25	1.0679819961016577
1113	1	-0.5331611544038743
1113	2	0.15579100480648833
1113	3	-1.0634664644681315
1113	4	-0.41642639865326103
1113	5	-0.040040398404944
1113	6	0.006705097575292029
1113	7	0.2950347401372433
1113	8	0.2484182196988911
1113	9	-0.3437825752235426
1113	10	0.17825965485171125
1113	11	-0.9401373562359687
1113	12	-0.9246000605860303
1113	13	0.10436024731582094
1113	14	0.551842269732628
1113	15	0.7317756067781751
1113	16	0.14930142678175784
1113	17	0.8444502019246727
1113	18	0.3820153767120473
1113	19	-0.12023233728714544
1113	20	0.21654525099080502
1113	21	-0.08124136043924679
1113	22	0.0793389144605996
1113	23	-0.13995899091566955
1113	24	-0.5317896426691391
1113	25	-0.03231966302352873
1114	1	-1.3097805876022548
1114	2	-1.0027110194850088
1114	3	-1.7057741567758242
1114	4	-1.3036733622160135
1114	5	-2.9867003174332822
1114	6	-1.1842868052587185
1114	7	-1.695552304397168
1114	8	-0.536095950341597
1114	9	-0.6500578788672655
1114	10	-0.4132383208567916
1114	11	-0.5532952509728091
1114	12	-0.2114421658491885
1114	13	-0.28956687819025007
1114	14	-5.808987689781542
1114	15	-2.3697426118453073
1114	16	-1.748674281720266
1114	17	-0.16364696406722956
1114	18	-6.062822679968114
1114	19	-2.8456371955867397
1114	20	-1.7868960445557533
1114	21	-0.5769903482934957
1114	22	-0.45304974950701293
1114	23	0.49364424795072637
1114	24	0.44331157190575965
1114	25	-0.17402006788183044
1115	1	-1.6943291148837645
1115	2	-1.2261779436762197
1115	3	-1.175498927418026
1115	4	-3.473060131892499
1115	5	-2.280954301016095
1115	6	-2.631703512163593
1115	7	-1.550501604252311
1115	8	-2.5021992820654013
1115	9	-1.1241283762115444
1115	10	-1.5475695618031935
1115	11	-1.846100658988263
1115	12	-1.555334005540157
1115	13	-1.7823722862057032
1115	14	-6.558866975150931
1115	15	-3.7552568561717194
1115	16	-2.6248693565633374
1115	17	-2.0129951050619184
1115	18	-1.6350100290748273
1115	19	-2.1023247861227112
1115	20	-2.0825020320014325
1115	21	-2.127642207298809
1115	22	-2.6761303868802635
1115	23	-2.248992159581867
1115	24	-2.3668284570657505
1115	25	-1.8495391407934676
1116	1	-2.7040484354396703
1116	2	-2.1909340523146064
1116	3	-2.010724651825331
1116	4	-1.546091288219138
1116	5	-3.5216664456250513
1116	6	-2.676939228395767
1116	7	-2.038647666564966
1116	8	-2.142766091039878
1116	9	-2.456311135771905
1116	10	-2.2844212286212517
1116	11	-2.759444286929039
1116	12	-3.5557881793979114
1116	13	-3.735319874542519
1116	14	-2.643797486550696
1116	15	-3.204343966717637
1116	16	-2.87811669964627
1116	17	-2.719144619095893
1116	18	-2.5891384694417994
1116	19	-3.4763823753678764
1116	20	-3.1343686865567975
1116	21	-4.282878831878698
1116	22	-2.902763141898884
1116	23	-3.9941097906110237
1116	24	-3.3811803509707374
1116	25	-2.825922100190322
1201	1	3.1783773071345855
1201	2	3.130951261216744
1201	3	3.192142509890843
1201	4	4.30312488339802
1201	5	3.7580365246719794
1201	6	3.9602307386009343
1201	7	2.8415090991116045
1201	8	3.2516233479040153
1201	9	3.486345629904662
1201	10	3.3385160651081236
1201	11	2.9140293104306956
1201	12	2.7524832727473054
1201	13	2.9152576832132553
1201	14	3.617547397937752
1201	15	3.4353012478038174
1201	16	3.374461683192017
1201	17	3.860475842950315
1201	18	4.765028197224865
1201	19	4.088902278097471
1201	20	4.666064481760035
1201	21	4.300970178022293
1201	22	4.9038581452298295
1201	23	5.251867932161254
1201	24	4.710197536818043
1201	25	4.3835457215918545
1202	1	1.8007999875469558
1202	2	1.8804143196359888
1202	3	1.8962876988942767
1202	4	2.0417417218859946
1202	5	1.7966963184864113
1202	6	1.8234180238242992
1202	7	1.3778063499707116
1202	8	2.033410289484774
1202	9	2.1560191694235655
1202	10	2.270646649300563
1202	11	2.227388416959908
1202	12	1.5109669497232474
1202	13	1.9921477175775202
1202	14	2.70539104054944
1202	15	2.1309627598313092
1202	16	2.0517597931576503
1202	17	2.358328076627288
1202	18	2.225294520248923
1202	19	2.1691213502624223
1202	20	1.4498918013476647
1202	21	1.604127394517139
1202	22	2.315262784405089
1202	23	2.7779632929859943
1202	24	1.7963229663713065
1202	25	1.9914709793238146
1203	1	1.2301449839022638
1203	2	1.7076558066712906
1203	3	1.1810945300928637
1203	4	2.171180438953577
1203	5	2.164602181237637
1203	6	1.1035388194090139
1203	7	1.8522414223439263
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2211	16	-7.075769798289468
2211	17	-4.033042675568208
2211	18	-3.1076569879603184
2211	19	-2.7594773515321585
2211	20	-3.1267595923140377
2211	21	-3.4594464886443745
2211	22	-3.6065585214368383
2211	23	-3.152067253023933
2211	24	-3.5189691298486245
2211	25	-8.481037611741478
2212	1	-0.4172109281595304
2212	2	-2.7495634446656076
2212	3	-0.24106827442288292
2212	4	-2.4137868813078605
2212	5	-0.25666935768096266
2212	6	0.4246915229146566
2212	7	0.3819502755821915
2212	8	0.4967213871197025
2212	9	1.2584044534340748
2212	10	1.402241555304201
2212	11	-0.043078532706555706
2212	12	0.4822626845120084
2212	13	-0.5479776109043897
2212	14	-3.234168288336754
2212	15	-2.673767379647167
2212	16	-1.290366748180535
2212	17	-4.85766141195165
2212	18	-1.981295332186896
2212	19	-0.7278869375888029
2212	20	-0.6066070868674167
2212	21	-0.45944648864437454
2212	22	-0.2683232273191898
2212	23	0.33213754000439266
2212	24	0.2506387132886303
2212	25	0.911119251003619
2213	1	0.05337730713458555
2213	2	-0.22668762767214545
2213	3	-0.015033416035084457
2213	4	-0.05104178326864428
2213	5	-0.11233384569839089
2213	6	-0.856898891028699
2213	7	-0.026546456443952593
2213	8	0.13125297753364862
2213	9	0.11134562990466534
2213	10	-4.957780231188174
2213	11	-2.495692911791526
2213	12	-1.2394148754008434
2213	13	-1.4690015760460007
2213	14	-0.6301377872474312
2213	15	-0.39108764108507543
2213	16	-0.5387327612524295
2213	17	0.23547584295031143
2213	18	0.27197264166931134
2213	19	-0.4909588330136394
2213	20	-0.37675959231403766
2213	21	-0.7372242664221496
2213	22	-0.967669632547949
2213	23	-0.04095614191281882
2213	24	-0.17637653725603286
2213	25	-0.0088153895192562
2214	1	-1.437363433606155
2214	2	-1.76372466470918
2214	3	-1.8020704530721225
2214	4	-1.4584491906760526
2214	5	-3.269741253105799
2214	6	-2.606898891028699
2214	7	-2.57284275274025
2214	8	-2.359487763207092
2214	9	-0.9534691849101513
2214	10	-5.078150601558544
2214	11	-3.079026245124858
2214	12	-2.0820074679934386
2214	13	-1.9412237982682221
2214	14	-1.7042118613215038
2214	15	-1.1503469003443314
2214	16	-1.9091031316227998
2214	17	-1.5700797126052422
2214	18	-0.7095088398121696
2214	19	-1.2965143885691965
2214	20	-1.4230558886103353
2214	21	-1.5520390812369662
2214	22	-1.263965928844243
2214	23	-1.3372524382091164
2214	24	-1.4078580187375138
2214	25	-1.5273339080377752
2215	1	-3.3994528815446614
2215	2	-5.256037732493947
2215	3	-4.081245854889033
2215	4	-3.5038719719478912
2215	5	-4.039133286648774
2215	6	-4.5275837268078725
2215	7	-5.307468888309781
2215	8	-5.179194262158877
2215	9	-8.498717263177095
2215	10	-5.942930475772382
2215	11	-5.727873205292571
2215	12	-5.750071758699239
2215	13	-4.9188618136420885
2215	14	-9.446524929106271
2215	15	-6.896852840246499
2215	16	-9.927621650141319
2215	17	-6.423329188496229
2215	18	-5.758949790196517
2215	19	-5.629847721902529
2215	20	-5.708345895598455
2215	21	-5.519194916317328
2215	22	-9.265363552883379
2215	23	-11.367825464065163
2215	24	-8.19035277764737
2215	25	-6.204308051993053
2216	1	-3.622548618791342
2216	2	-3.717428368412886
2216	3	-3.700218601220268
2216	4	-7.504745486972347
2216	5	-6.12159310495765
2216	6	-4.430972965102772
2216	7	-4.7580279379254335
2216	8	-7.683561837281168
2216	9	-5.675691407132373
2216	10	-4.374446897854842
2216	11	-4.310507726606339
2216	12	-4.239414875400843
2216	13	-3.5986312056756304
2216	14	-3.509767416877061
2216	15	-3.0855320855295183
2216	16	-3.816510539030208
2216	17	-3.1071167496422802
2216	18	-8.5891384694418
2216	19	-6.129847721902529
2216	20	-6.469352184906633
2216	21	-5.015002044199928
2216	22	-4.884336299214613
2216	23	-3.679845030801708
2216	24	-4.565265426144922
2216	25	-4.092148722852588
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8093.0
3	1.0
4	49.0
5	376.0
6	653.0
7	540.0
8	463.0
9	351.0
10	304.0
11	287.0
12	319.0
13	249.0
14	228.0
15	193.0
16	182.0
17	184.0
18	223.0
19	271.0
20	422.0
21	572.0
22	659.0
23	752.0
24	800.0
25	786.0
26	790.0
27	801.0
28	898.0
29	1015.0
30	1175.0
31	1332.0
32	1660.0
33	2115.0
34	2727.0
35	3548.0
36	5215.0
37	7639.0
38	17928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.03847663301989	18.00442693207328	11.004536820458078	21.952559614448752
2	13.610648395511834	24.570198321651947	35.98547946066568	25.83367382217054
3	21.85314960011313	31.4818597489119	26.420816114890876	20.244174536084095
4	12.732997481108313	19.330919395465994	31.05478589420655	36.88129722921914
5	11.522026016464526	41.80544209137184	32.35562119022183	14.31691070194181
6	31.709649604138907	31.74414047189778	18.438504350552638	18.107705573410676
7	24.403856706122127	34.5426040605158	21.594418750489925	19.45912048287215
8	29.043990844385913	32.45539773618035	21.473364061079234	17.027247358354497
9	29.47936099832254	14.987379873641965	18.803204414692647	36.730054713342845
10	17.775617709770476	31.96256114386053	29.251849993728836	21.009971152640162
11	29.48812416712393	28.044211021400017	17.35360978286431	25.11405502861174
12	25.13052885746092	32.13440160554414	26.478935072672822	16.256134464322113
13	30.40812806723216	23.288229668072564	21.453770049702882	24.849872214992395
14	21.92154526355398	28.73694772819918	25.176382051362456	24.165124956884387
15	33.213109612670536	24.381370550415557	21.442684647953584	20.962835188960327
16	19.099132997820735	29.71794992396093	30.31685558847969	20.866061489738644
17	23.58088189173932	31.065357837295366	26.309352066737752	19.04440820422756
18	24.126630707476167	25.631899147014554	29.93602609131962	20.305444054189664
19	21.922039638735573	31.334671349724037	30.180632212744605	16.562656798795786
20	24.67856246864024	27.039952333166084	27.036816357250377	21.2446688409433
21	21.29831438651509	29.345354762838106	29.74362994903959	19.612700901607212
22	22.06435829883327	29.35798519633672	23.057019194580352	25.520637310249654
23	18.257365507941735	30.33695532872352	28.221772739388808	23.183906423945935
24	21.388244150304246	34.492817263659745	24.502854275139576	19.616084310896433
25	21.417819173958037	26.45121836485088	23.009376862044093	29.121585599146986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	297.0
1	297.0
2	259.0
3	221.0
4	221.0
5	221.0
6	466.0
7	711.0
8	711.0
9	711.0
10	628.5
11	546.0
12	546.0
13	546.0
14	482.5
15	419.0
16	419.0
17	419.0
18	565.5
19	712.0
20	712.0
21	712.0
22	962.0
23	1212.0
24	1212.0
25	1212.0
26	1668.0
27	2124.0
28	2124.0
29	2124.0
30	2578.5
31	3033.0
32	3033.0
33	3033.0
34	3328.0
35	3623.0
36	3623.0
37	3623.0
38	3972.5
39	4322.0
40	4322.0
41	4322.0
42	5281.0
43	6240.0
44	6240.0
45	6240.0
46	10837.5
47	15435.0
48	15435.0
49	15435.0
50	13504.0
51	11573.0
52	11573.0
53	11573.0
54	8654.0
55	5735.0
56	5735.0
57	5735.0
58	4487.5
59	3240.0
60	3240.0
61	3240.0
62	2687.0
63	2134.0
64	2134.0
65	2134.0
66	1643.5
67	1153.0
68	1153.0
69	1153.0
70	881.5
71	610.0
72	610.0
73	610.0
74	446.0
75	282.0
76	282.0
77	282.0
78	198.0
79	114.0
80	114.0
81	114.0
82	77.5
83	41.0
84	41.0
85	41.0
86	29.0
87	17.0
88	17.0
89	17.0
90	11.0
91	5.0
92	5.0
93	5.0
94	3.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15517241379310345
2	0.2601880877742947
3	0.24608150470219436
4	0.438871473354232
5	0.23197492163009406
6	0.023510971786833857
7	0.023510971786833857
8	0.0219435736677116
9	0.02037617554858934
10	0.025078369905956112
11	0.023510971786833857
12	0.0329153605015674
13	0.0329153605015674
14	0.02821316614420063
15	0.047021943573667714
16	0.02664576802507837
17	0.0438871473354232
18	0.037617554858934164
19	0.037617554858934164
20	0.037617554858934164
21	0.03918495297805642
22	0.050156739811912224
23	0.03605015673981191
24	0.05642633228840126
25	0.04075235109717868
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	63800.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.29780564263322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.53414929853935	48.07210031347962
2	6.740372957681427	7.31974921630094
3	1.9975751977368512	3.2539184952978055
4	0.82270076785405	1.7868338557993733
5	0.4099070492465793	1.1128526645768027
6	0.2511402343975521	0.8181818181818182
7	0.1732001616534842	0.658307210031348
8	0.14144679868367876	0.6144200626959248
9	0.10680676635298192	0.5219435736677116
>10	0.65816061428324	6.636363636363636
>50	0.03752670169158825	1.3338557993730409
>100	0.09526008890941631	10.888714733542319
>500	0.02309335488713123	8.04858934169279
>1k	0.00866000808267421	8.934169278996865
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2414	3.7836990595611284	No Hit
CCCATGTACTCTGCGTTGATACCAC	2264	3.54858934169279	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1022	1.6018808777429467	No Hit
CATGTACTCTGCGTTGATACCACTG	816	1.2789968652037618	No Hit
TATCAACGCAGAGTACATGGGAAGC	704	1.103448275862069	No Hit
GCGTTGATACCACTGCTTCCCATGT	677	1.061128526645768	No Hit
GTATCAACGCAGAGTACATGGGAAG	631	0.9890282131661442	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	595	0.9326018808777429	No Hit
GGTATCAACGCAGAGTACATGGGAA	576	0.9028213166144201	No Hit
GTATCAACGCAGAGTACTTTTTTTT	568	0.890282131661442	No Hit
ACTCTGCGTTGATACCACTGCTTCC	568	0.890282131661442	No Hit
TATCAACGCAGAGTACTTTTTTTTT	406	0.6363636363636364	No Hit
GGTATCAACGCAGAGTACTTTTTTT	387	0.6065830721003135	No Hit
GTACTCTGCGTTGATACCACTGCTT	310	0.48589341692789967	No Hit
GCTTCCCATGTACTCTGCGTTGATA	304	0.4764890282131662	No Hit
GTGGTATCAACGCAGAGTACATGGG	303	0.47492163009404387	No Hit
ATACCACTGCTTCCCATGTACTCTG	274	0.4294670846394984	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	267	0.41849529780564265	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	266	0.41692789968652033	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	262	0.4106583072100313	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	256	0.4012539184952978	No Hit
TACCACTGCTTCCCATGTACTCTGC	228	0.3573667711598746	No Hit
GCAGTGGTATCAACGCAGAGTACAT	226	0.3542319749216301	No Hit
GATACCACTGCTTCCCATGTACTCT	222	0.34796238244514105	No Hit
GTTGATACCACTGCTTCCCATGTAC	214	0.335423197492163	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	208	0.32601880877742945	No Hit
CCACTGCTTCCCATGTACTCTGCGT	207	0.32445141065830724	No Hit
CAGTGGTATCAACGCAGAGTACATG	206	0.3228840125391849	No Hit
CATGGGAAGCAGTGGTATCAACGCA	203	0.3181818181818182	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	195	0.3056426332288401	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	187	0.2931034482758621	No Hit
ACATGGGAAGCAGTGGTATCAACGC	184	0.2884012539184953	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	182	0.2852664576802508	No Hit
CTTCCCATGTACTCTGCGTTGATAC	162	0.25391849529780564	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	161	0.25235109717868337	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	161	0.25235109717868337	No Hit
GTACATGGGAAGCAGTTGTATCAAC	135	0.2115987460815047	No Hit
TTCCCATGTACTCTGCGTTGATACC	131	0.20532915360501566	No Hit
GTACATGGGAAGCAGTGTTATCAAC	123	0.19278996865203762	No Hit
TGATACCACTGCTTCCCATGTACTC	122	0.19122257053291536	No Hit
CCATGTACTCTGCGTTGATACCACT	122	0.19122257053291536	No Hit
CCCATGTACTCTGCGTTTATACCAC	121	0.1896551724137931	No Hit
ATCAACGCAGAGTACATGGGAAGCA	107	0.1677115987460815	No Hit
GTACATGGTAAGCAGTGGTATCAAC	105	0.164576802507837	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	78	0.12225705329153606	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	75	0.11755485893416928	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	74	0.11598746081504703	No Hit
ACCATGTACTCTGCGTTGATACCAC	73	0.11442006269592477	No Hit
AAAAAGTACTCTGCGTTGATACCAC	68	0.10658307210031348	No Hit
ATTCCATTCCATTCCATTCCATTCC	67	0.10501567398119123	No Hit
GAGTACATGTGAAGCAGTGGTATCA	65	0.10188087774294671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.001567398119122257	0.0
9	0.0	0.0	0.0	0.001567398119122257	0.0
10	0.0	0.0	0.0	0.001567398119122257	0.0
11	0.0	0.0	0.0	0.001567398119122257	0.0
12	0.0	0.0	0.0	0.001567398119122257	0.0
13	0.0	0.0	0.0	0.001567398119122257	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACAAA	50	4.206904E-6	17.190851	1
AACAAAA	80	1.8527771E-6	13.100904	2
CATGGTA	60	3.9251655E-4	12.69392	4
ACATGGT	75	0.0025426159	10.155136	3
TGGTAAG	80	0.0044523515	9.490596	6
ACAAAAA	125	2.5591053E-5	9.139622	3
ATGGTAA	85	0.0071919593	8.953376	5
GGTAAGC	85	0.007332641	8.932325	7
GGAAGCA	695	0.0	8.329875	8
GAAGCAG	690	0.0	8.252691	9
AAGCAGT	780	0.0	7.787155	10
GTGGTAT	720	0.0	7.5133886	15
TACATGG	1225	0.0	7.4667864	2
GTACATG	1260	0.0	7.428146	1
AGTGGTA	730	0.0	7.410465	14
CAGTGGT	730	0.0	7.410465	13
TGGTATC	745	0.0	7.3886514	16
AGCAGTG	780	0.0	7.300458	11
TGGGAAG	785	0.0	7.253958	6
GGGAAGC	775	0.0	7.2250986	7
>>END_MODULE