FastQCFastQC Report
Thu 26 May 2016
SRR1513199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63800
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC28534.471786833855799No Hit
GTACATGGGAAGCAGTGGTATCAAC28314.43730407523511No Hit
GTATCAACGCAGAGTACTTTTTTTT11731.8385579937304073No Hit
GAGTACATGGGAAGCAGTGGTATCA11191.7539184952978057No Hit
CATGTACTCTGCGTTGATACCACTG9651.512539184952978No Hit
TATCAACGCAGAGTACTTTTTTTTT9651.512539184952978No Hit
TATCAACGCAGAGTACATGGGAAGC8511.3338557993730409No Hit
GGTATCAACGCAGAGTACTTTTTTT8001.2539184952978055No Hit
ACGCAGAGTACATGGGAAGCAGTGG7751.2147335423197492No Hit
GTATCAACGCAGAGTACATGGGAAG7571.1865203761755487No Hit
GCGTTGATACCACTGCTTCCCATGT6991.0956112852664577No Hit
CTTATACACATCTCCGAGCCCACGA6711.0517241379310345No Hit
GGTATCAACGCAGAGTACATGGGAA6611.036050156739812No Hit
ACTCTGCGTTGATACCACTGCTTCC5890.9231974921630094No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5470.8573667711598745No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5050.7915360501567399No Hit
ATACACATCTCCGAGCCCACGAGAC4080.6394984326018809No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3510.5501567398119123No Hit
ATACCACTGCTTCCCATGTACTCTG3480.5454545454545455No Hit
GTGGTATCAACGCAGAGTACATGGG3290.5156739811912225No Hit
GTACTCTGCGTTGATACCACTGCTT3170.49686520376175547No Hit
GCTTCCCATGTACTCTGCGTTGATA3140.49216300940438873No Hit
GGGAAGCAGTGGTATCAACGCAGAG3070.48119122257053293No Hit
ACACATCTCCGAGCCCACGAGACTA3040.4764890282131662No Hit
CATGGGAAGCAGTGGTATCAACGCA2980.4670846394984326No Hit
ACATGGGAAGCAGTGGTATCAACGC2810.44043887147335425No Hit
CAGTGGTATCAACGCAGAGTACATG2810.44043887147335425No Hit
GCAGAGTACATGGGAAGCAGTGGTA2780.43573667711598746No Hit
TACCACTGCTTCCCATGTACTCTGC2740.4294670846394984No Hit
CTGCTTCCCATGTACTCTGCGTTGA2730.4278996865203762No Hit
GATACCACTGCTTCCCATGTACTCT2620.4106583072100313No Hit
CCACTGCTTCCCATGTACTCTGCGT2460.38557993730407525No Hit
GCAGTGGTATCAACGCAGAGTACAT2450.384012539184953No Hit
AAGCAGTGGTATCAACGCAGAGTAC2090.3275862068965517No Hit
ATGGGAAGCAGTGGTATCAACGCAG2030.3181818181818182No Hit
GTTGATACCACTGCTTCCCATGTAC2010.3150470219435737No Hit
CTTCCCATGTACTCTGCGTTGATAC1960.3072100313479624No Hit
CCATGTACTCTGCGTTGATACCACT1830.28683385579937304No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1770.2774294670846395No Hit
TTCCCATGTACTCTGCGTTGATACC1680.2633228840125392No Hit
ATCAACGCAGAGTACATGGGAAGCA1550.24294670846394983No Hit
TGATACCACTGCTTCCCATGTACTC1500.23510971786833856No Hit
ATCAACGCAGAGTACTTTTTTTTTT1250.1959247648902821No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1220.19122257053291536No Hit
AAAAAGTACTCTGCGTTGATACCAC1080.16927899686520376No Hit
GTGGTATCAACGCAGAGTACTTTTT1070.1677115987460815No Hit
GTACATGGTAAGCAGTGGTATCAAC980.1536050156739812No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA970.15203761755485892No Hit
ATTCCATTCCATTCCATTCCATTCC870.13636363636363638No Hit
GGAAGCAGTGGTATCAACGCAGAGT860.13479623824451412No Hit
CATCTCCGAGCCCACGAGACTAGGC850.13322884012539185No Hit
CACATCTCCGAGCCCACGAGACTAG800.12539184952978058No Hit
GTACTGGTTCACTATCGGTCAGTCA770.1206896551724138No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT760.11912225705329155No Hit
CTGCGTTGATACCACTGCTTCCCAT740.11598746081504703No Hit
ACCATGTACTCTGCGTTGATACCAC740.11598746081504703No Hit
AAAGTACTCTGCGTTGATACCACTG720.11285266457680251No Hit
TCAACGCAGAGTACATGGGAAGCAG700.109717868338558No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT700.109717868338558No Hit
TGGGAAGCAGTGGTATCAACGCAGA680.10658307210031348No Hit
CCCCATGTACTCTGCGTTGATACCA640.10031347962382445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC659.0058165E-917.6156271
TTATACA806.848495E-915.5054222
TATACAC801.15944204E-714.3126973
ATACACA1105.512251E-610.40103154
CCATGTA7600.09.7928982
TTGATAC7750.09.66549716
TGATACC7950.09.54161117
TACACAT1201.5521515E-59.5342795
CCCATGT7250.09.4759921
TGCGTTG7850.09.42157912
CTGCGTT7900.09.24192511
ATACCAC7900.09.24192519
TACTCTG8150.09.1983267
CTCTGCG8100.09.1307919
CGTTGAT8300.09.02501314
GATACCA8100.09.01372918
TCTGCGT8100.09.01372910
ACTCTGC8400.08.9175728
GTACTCT8450.08.9137056
TGTACTC8300.08.8450545
Produced by FastQC (version 0.11.5)