FastQCFastQC Report
Thu 26 May 2016
SRR1513198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428729
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18140.12696599565067973No Hit
GTACATGGGAAGCAGTGGTATCAAC17290.12101665186329948No Hit
GTATCAACGCAGAGTACTTTTTTTT17030.11919685258715963No Hit
CCCATGTACTCTGCGTTGATACCAC15790.11051780988556963No Hit
GTCCTAAAGTGTGTATTTCTCATTT15080.10554835801611083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGGA400.005106721514.321361
CGCTCGC400.005313092414.23610818
TAGGACC5250.013.6077534
ACCGTCT604.1327838E-412.651668
CGGTAGG550.002955543612.1514571
CTGTCCG658.0933835E-411.680099
TGGCGAG10750.011.47717118
GGCGAGG4800.011.46797619
AGGACCT9050.011.4536955
AGGGCCG600.005830706611.0946725
TTAGGAC7300.010.9607653
GGACCTG9150.010.8879616
ACTCCGT700.001506973410.8435216
GTCCTAA5150.010.7526061
TGTAGGA11350.010.7487542
GTAGGAC11200.010.7161053
CTACACT1605.18412E-1010.7161054
TAGTACC803.6782655E-410.7161054
GGCGAGA6500.010.65882919
GTCCTAC10600.010.6284291
Produced by FastQC (version 0.11.5)