##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513198_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1428729 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.102872553157386 31.0 30.0 34.0 23.0 34.0 2 28.948049630125798 31.0 30.0 34.0 16.0 34.0 3 29.14967009138892 31.0 30.0 34.0 22.0 34.0 4 32.22932200578276 35.0 33.0 37.0 19.0 37.0 5 32.36776813517469 35.0 33.0 37.0 22.0 37.0 6 32.71281817615517 36.0 33.0 37.0 25.0 37.0 7 32.668240793040525 37.0 33.0 37.0 25.0 37.0 8 32.55024640782122 37.0 33.0 37.0 23.0 37.0 9 33.71964522313189 38.0 34.0 39.0 21.0 39.0 10 33.30309246890068 38.0 33.0 39.0 17.0 39.0 11 33.73453048128791 38.0 34.0 39.0 23.0 39.0 12 33.718629635151245 38.0 34.0 39.0 22.0 39.0 13 33.750156957687565 38.0 34.0 39.0 22.0 39.0 14 33.95322275952962 39.0 33.0 41.0 11.0 41.0 15 34.35297736659646 39.0 33.0 41.0 17.0 41.0 16 34.1931940906918 39.0 33.0 41.0 17.0 41.0 17 33.822998623251856 39.0 33.0 41.0 11.0 41.0 18 33.546884678619946 38.0 32.0 41.0 11.0 41.0 19 34.17027161904042 39.0 33.0 41.0 18.0 41.0 20 34.37739207365428 39.0 34.0 41.0 18.0 41.0 21 34.41414641964991 39.0 34.0 41.0 18.0 41.0 22 34.10686561272291 39.0 34.0 41.0 10.0 41.0 23 33.6191762048646 39.0 33.0 41.0 8.0 41.0 24 33.84741752984646 39.0 34.0 41.0 2.0 41.0 25 33.45765152103723 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0532866346990417 1101 2 1.258456625419285 1101 3 1.2480650904668913 1101 4 1.5509607518539141 1101 5 1.436864438528339 1101 6 1.2129355419470187 1101 7 1.003729336435093 1101 8 1.1689259709048727 1101 9 1.5296605073130891 1101 10 1.8357667263210828 1101 11 1.460400296957424 1101 12 1.2626307178246563 1101 13 1.1101017160911937 1101 14 2.150815913255343 1101 15 1.875248238215498 1101 16 1.8866679192139841 1101 17 2.2088331713397906 1101 18 2.369271633488566 1101 19 1.930426189959313 1101 20 1.509935633760442 1101 21 1.6321740244116754 1101 22 1.7812779832142667 1101 23 2.0380090275604914 1101 24 1.8375703201975497 1101 25 2.0280970016222497 1102 1 1.268566252507867 1102 2 1.3721745586964147 1102 3 1.276877969346014 1102 4 1.6940536098570433 1102 5 1.6208662049581477 1102 6 1.3350681044746224 1102 7 1.3585850110908382 1102 8 1.3740004419795824 1102 9 1.8324546780947344 1102 10 1.900229370345329 1102 11 1.480332851455799 1102 12 1.5800902705967417 1102 13 1.4474456480033524 1102 14 2.5982284950530854 1102 15 2.1125279363165888 1102 16 2.213341009261761 1102 17 2.454760346256485 1102 18 2.775490269323008 1102 19 2.21639110224001 1102 20 1.8937326549174571 1102 21 1.6685745222237074 1102 22 2.0498596203735246 1102 23 2.324086349010571 1102 24 2.2139242484965607 1102 25 2.447816781621448 1103 1 0.8904806153833285 1103 2 1.0314196161108065 1103 3 0.9210293289435967 1103 4 1.379446439757885 1103 5 1.1636631208671915 1103 6 0.8594604602170932 1103 7 0.9875468104187703 1103 8 0.9393322497599073 1103 9 1.423583793480141 1103 10 1.6420243690053198 1103 11 1.1586895834738584 1103 12 1.0260743553680172 1103 13 1.0568587224049821 1103 14 2.0165866701618285 1103 15 1.5342248048097957 1103 16 1.5418636967089299 1103 17 1.9849580496807349 1103 18 2.1779899085503303 1103 19 1.6519055640702618 1103 20 1.343890364114067 1103 21 1.2383355876369535 1103 22 1.4952881152299398 1103 23 1.8777023219629285 1103 24 1.5925252751525036 1103 25 1.870741307978463 1104 1 0.7514011461887122 1104 2 0.9258282851873254 1104 3 0.840577442158672 1104 4 1.2097534961499719 1104 5 1.1213554899413083 1104 6 0.9569588443408037 1104 7 0.939864894667771 1104 8 1.004832692629698 1104 9 1.359031730345265 1104 10 1.5574645656235475 1104 11 1.1142122895280053 1104 12 0.9526852443792606 1104 13 1.0348109882226169 1104 14 1.9821728941182926 1104 15 1.4885521613957877 1104 16 1.7644783840268943 1104 17 2.1745988862657697 1104 18 2.129028059189899 1104 19 1.6210155952706415 1104 20 1.4641307107494512 1104 21 1.40752780908781 1104 22 1.5269840272624293 1104 23 1.8640838762364211 1104 24 1.6487601637168936 1104 25 1.7964078070727751 1105 1 0.7446532090296039 1105 2 0.8632421827791106 1105 3 0.7847766515838401 1105 4 1.2058227252993206 1105 5 1.2454341841264522 1105 6 0.8076357680112736 1105 7 0.7894458964376412 1105 8 1.0201852443152575 1105 9 1.1794837951151749 1105 10 1.5026005531749718 1105 11 0.9958382566479358 1105 12 1.1742905388836533 1105 13 1.1024760275504164 1105 14 1.9362936729904305 1105 15 1.440090470838797 1105 16 1.7694090278840235 1105 17 1.9831987586292428 1105 18 1.4514746455559404 1105 19 1.286899269209158 1105 20 1.3126547728058782 1105 21 1.1503546847740154 1105 22 1.315171701175231 1105 23 1.764487201216042 1105 24 1.5384172651461299 1105 25 1.9279043694064981 1106 1 0.6913353370114308 1106 2 0.9662963093508097 1106 3 0.8741881532355151 1106 4 1.332983610021671 1106 5 1.2041056317233654 1106 6 0.7955240417842688 1106 7 0.646451308441975 1106 8 0.768109228177174 1106 9 0.9611283524275791 1106 10 0.8107844960388277 1106 11 0.8684382710103122 1106 12 0.8727842847218312 1106 13 0.6049094709953025 1106 14 1.720382282320756 1106 15 1.3096825467243676 1106 16 1.4497425287784083 1106 17 1.6863831258360236 1106 18 1.803129256531406 1106 19 1.4238787962952983 1106 20 1.098931196313174 1106 21 1.0227229426178326 1106 22 1.2163960937120208 1106 23 1.598085204845809 1106 24 1.2981518549697313 1106 25 1.6098389487847413 1107 1 0.6265275738820151 1107 2 0.9089634374354993 1107 3 0.8441070766508894 1107 4 1.2047345702772674 1107 5 1.042155376032099 1107 6 0.7369456238719891 1107 7 0.7124935128463932 1107 8 0.8427224608061579 1107 9 1.0289378228575217 1107 10 1.3185238815398534 1107 11 0.9197321932142657 1107 12 0.8886226468116831 1107 13 0.7539909691555522 1107 14 1.5852541701719431 1107 15 1.0928604274103932 1107 16 1.2059698549551854 1107 17 1.7158500103436864 1107 18 1.8458355943798566 1107 19 1.4379494885515456 1107 20 1.202048836911068 1107 21 0.9386434392694554 1107 22 1.3915845913198623 1107 23 1.7002125061480058 1107 24 1.4955556517347475 1107 25 1.855392416203891 1108 1 0.5617782148362664 1108 2 0.8279360796175617 1108 3 0.7294980359559915 1108 4 1.1672516765922438 1108 5 1.0601139847937802 1108 6 0.809929199035345 1108 7 0.6076445339145522 1108 8 0.6331980926548724 1108 9 0.9011829283608321 1108 10 1.1401307277912878 1108 11 0.722031345359305 1108 12 0.5170069030966786 1108 13 0.6327395458586196 1108 14 1.6475560331700265 1108 15 1.054572204283403 1108 16 1.1327326327234957 1108 17 1.544158291405246 1108 18 1.8051143410145727 1108 19 1.0892345081953252 1108 20 0.9837510544601926 1108 21 0.840273359477635 1108 22 1.139179402810548 1108 23 1.374398090988791 1108 24 1.1408948274055533 1108 25 1.592127566257929 1109 1 0.7586882967489039 1109 2 0.946545268078566 1109 3 0.8654935299867432 1109 4 1.0592710011613917 1109 5 1.2663658235698918 1109 6 0.8564258466561583 1109 7 0.8937108692144093 1109 8 1.0033673374792045 1109 9 1.2123936559742177 1109 10 1.4669532452499823 1109 11 1.053959853744125 1109 12 1.1033740234571567 1109 13 0.8980749386211997 1109 14 1.9497540480660547 1109 15 1.6049573794897327 1109 16 1.7553890641816707 1109 17 2.0023003920230735 1109 18 1.9629235263786882 1109 19 1.508934961889139 1109 20 1.2689614878694044 1109 21 1.2313891675326332 1109 22 1.4536925723186087 1109 23 1.8325150294072117 1109 24 1.5099387412501812 1109 25 1.8528892453895622 1110 1 0.36143537797510206 1110 2 0.5403877479437078 1110 3 0.41915058186911835 1110 4 0.8041202238622702 1110 5 0.6421368793231537 1110 6 0.362413235055989 1110 7 0.27661497110614874 1110 8 0.4119301145285874 1110 9 0.515440418788728 1110 10 0.8627795547960915 1110 11 0.47691359039892944 1110 12 0.3443929408574178 1110 13 0.15697173577422774 1110 14 1.1347762120168525 1110 15 0.8350816224815603 1110 16 1.0386864981580644 1110 17 1.276677616222365 1110 18 0.908937136917487 1110 19 0.5260511509382724 1110 20 0.4295112162829611 1110 21 0.31010709223763655 1110 22 0.7084593825827028 1110 23 -1.002226183054546 1110 24 -0.15027072369331052 1110 25 0.336164827583481 1111 1 0.30748546457007464 1111 2 0.6823931957245257 1111 3 0.5690715338069658 1111 4 0.8918717421919311 1111 5 0.7992400401563913 1111 6 0.4927094941220034 1111 7 0.39616524520685914 1111 8 0.5575438807174891 1111 9 0.8912750527324036 1111 10 1.0873248728322764 1111 11 0.7210236928346347 1111 12 0.6129297503344731 1111 13 0.3185493592595918 1111 14 1.2754603667705524 1111 15 0.743386064889286 1111 16 0.9401156653369398 1111 17 1.2211788353986748 1111 18 1.6580690475931803 1111 19 1.061414460811342 1111 20 0.6369734778355749 1111 21 0.4677836627343481 1111 22 0.7721636790316069 1111 23 1.2990854234728317 1111 24 0.9562119176323307 1111 25 1.0570619530660537 1112 1 0.33797755553060327 1112 2 0.5129501444119384 1112 3 0.42655612522763064 1112 4 0.8513469594312326 1112 5 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25 0.4810961461791905 2210 1 0.20927209540190717 2210 2 0.2569258210801557 2210 3 0.14661556707776668 2210 4 0.5144769751931904 2210 5 0.2888129191529032 2210 6 0.1471986884325247 2210 7 0.15438294779176687 2210 8 0.15102803160907285 2210 9 0.24637731559194265 2210 10 0.5292165675950429 2210 11 0.20439658148166728 2210 12 0.02027185495537509 2210 13 -0.12061913892460296 2210 14 -5.666981388736435 2210 15 -2.1361535198292927 2210 16 -0.6811273173131838 2210 17 -0.15872432037573247 2210 18 0.7521652982561875 2210 19 0.25249093853719273 2210 20 0.12763269187002635 2210 21 -0.1784049705607842 2210 22 0.18654358032434715 2210 23 0.4323150727623002 2210 24 0.3749939816092862 2210 25 0.12400616209546911 2211 1 -0.2903441374712372 2211 2 -0.22700742118514583 2211 3 -0.216482865407702 2211 4 -0.3384769856403338 2211 5 -0.03815704651632501 2211 6 -0.2336862156126216 2211 7 -0.40157859293285014 2211 8 -0.44709913998143946 2211 9 -0.1944077623018572 2211 10 -0.15503022614221607 2211 11 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-0.8323698778996693 2214 9 -0.6378116445110393 2214 10 -4.155642872704476 2214 11 -1.7627422739154497 2214 12 -1.2891623707197724 2214 13 -0.9222442103149717 2214 14 -0.10858488437142455 2214 15 -0.4253132438771914 2214 16 -0.5345269492561826 2214 17 -0.15397620372160503 2214 18 0.12419079028313007 2214 19 -0.682466680514743 2214 20 -0.778087784247063 2214 21 -0.8105929153341052 2214 22 -0.47463329554253164 2214 23 -0.24218075260100136 2214 24 -0.8473942188095407 2214 25 -0.49288529735027 2215 1 -2.289039326351027 2215 2 -4.9341688194249045 2215 3 -3.1274974051718907 2215 4 -2.0312155403241334 2215 5 -1.8731479432725209 2215 6 -2.102193460839292 2215 7 -2.137826453882436 2215 8 -2.5001229347743674 2215 9 -8.11816164264139 2215 10 -3.9772571269018364 2215 11 -2.979388125166242 2215 12 -3.5177919186388564 2215 13 -2.907065658918345 2215 14 -8.294757714644767 2215 15 -5.051604609842119 2215 16 -9.20562518056905 2215 17 -4.579377991105222 2215 18 -3.038029977768325 2215 19 -3.4622602713609503 2215 20 -3.3692241563818257 2215 21 -3.329904884821449 2215 22 -9.56603964486759 2215 23 -10.494072088651556 2215 24 -6.702997928191433 2215 25 -5.098263901097042 2216 1 -2.434319708701615 2216 2 -2.63178891689844 2216 3 -2.285668963625927 2216 4 -7.4021495404839825 2216 5 -4.227664154287375 2216 6 -3.371865756137229 2216 7 -2.9788747209746553 2216 8 -8.864781189177243 2216 9 -5.061375718919326 2216 10 -3.167993952842334 2216 11 -2.9816431099758525 2216 12 -3.1446970036251116 2216 13 -2.8771550051703194 2216 14 -2.1381781610976347 2216 15 -2.8370754939560143 2216 16 -2.6082670421034635 2216 17 -2.3797512175834648 2216 18 -8.136331285408062 2216 19 -5.8059900934677025 2216 20 -5.203687975595084 2216 21 -4.299295553979885 2216 22 -3.9409292978908965 2216 23 -3.1039779019503086 2216 24 -3.525570541665843 2216 25 -2.8788855800234856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71184.0 3 0.0 4 615.0 5 3499.0 6 4419.0 7 3908.0 8 3706.0 9 3465.0 10 3177.0 11 3079.0 12 2944.0 13 2994.0 14 2940.0 15 3037.0 16 3069.0 17 3230.0 18 3808.0 19 4541.0 20 5944.0 21 7525.0 22 9537.0 23 11333.0 24 13022.0 25 15034.0 26 16788.0 27 18691.0 28 20814.0 29 24848.0 30 29628.0 31 35422.0 32 44960.0 33 57901.0 34 75200.0 35 98492.0 36 137044.0 37 195413.0 38 487518.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22441046467275 18.72510882003186 12.494110256007538 25.556370459287848 2 14.556858563074645 20.201847285024222 41.21887135305033 24.022422798850794 3 18.66365331802803 24.353044525243224 29.717151248865083 27.26615090786366 4 11.621057513914657 16.450286726260753 38.79497666835329 33.1336790914713 5 12.663345211709427 38.100329830159566 36.200280787130104 13.036044171000905 6 32.3170578039376 37.03940681169687 17.870203656463776 12.773331727901748 7 28.174274265585055 31.51249856491217 22.097832387342105 18.21539478216067 8 25.046254650841266 36.763679511097266 20.624499039205325 17.565566798856146 9 26.36931688514973 14.870359247483 20.215108863510036 38.54521500385723 10 13.914962252738636 28.949321221353525 35.61501713748068 21.52069938842716 11 34.48269344259885 22.3920803501985 24.45340989200159 18.671816315201053 12 22.930595831017154 24.82192839610238 31.78075804525196 20.46671772762851 13 28.70764959033179 21.75193632377476 25.782388222502732 23.75802586339072 14 20.364356058564127 21.889437216768894 28.556136554411527 29.190070170255456 15 23.063888425973257 30.73145182635389 23.365188153031973 22.839471594640884 16 22.087662912711618 28.18133514042153 27.89774083638968 21.833261110477167 17 21.500496790641428 28.486024393505797 28.29851130855145 21.714967507301317 18 21.43586785064409 27.589087113078335 29.796547347077944 21.178497689199624 19 23.202247623661655 27.332319111592163 27.650167710642958 21.81526555410322 20 23.542878746508162 27.372245909558718 27.87339060301191 21.211484740921215 21 22.968781506956738 26.9730356462707 27.256318195057467 22.8018646517151 22 22.97581169194918 28.001971812050456 27.164511864482737 21.857704631517624 23 22.105489406181892 27.640893265085943 27.90931806518508 22.344299263547086 24 22.835821209067692 27.148808277529152 28.041978657744533 21.973391855658626 25 22.601295739658976 27.223275982184802 27.88213852357376 22.293289754582453 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 115.0 1 115.0 2 210.5 3 306.0 4 306.0 5 306.0 6 673.0 7 1040.0 8 1040.0 9 1040.0 10 1563.0 11 2086.0 12 2086.0 13 2086.0 14 3904.0 15 5722.0 16 5722.0 17 5722.0 18 10746.0 19 15770.0 20 15770.0 21 15770.0 22 25532.0 23 35294.0 24 35294.0 25 35294.0 26 50558.5 27 65823.0 28 65823.0 29 65823.0 30 85511.5 31 105200.0 32 105200.0 33 105200.0 34 121618.5 35 138037.0 36 138037.0 37 138037.0 38 150898.5 39 163760.0 40 163760.0 41 163760.0 42 172327.0 43 180894.0 44 180894.0 45 180894.0 46 186200.0 47 191506.0 48 191506.0 49 191506.0 50 182922.0 51 174338.0 52 174338.0 53 174338.0 54 154715.5 55 135093.0 56 135093.0 57 135093.0 58 115368.0 59 95643.0 60 95643.0 61 95643.0 62 77749.5 63 59856.0 64 59856.0 65 59856.0 66 46278.0 67 32700.0 68 32700.0 69 32700.0 70 23831.5 71 14963.0 72 14963.0 73 14963.0 74 10703.0 75 6443.0 76 6443.0 77 6443.0 78 4552.0 79 2661.0 80 2661.0 81 2661.0 82 1825.0 83 989.0 84 989.0 85 989.0 86 683.5 87 378.0 88 378.0 89 378.0 90 233.0 91 88.0 92 88.0 93 88.0 94 52.0 95 16.0 96 16.0 97 16.0 98 12.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17645053750571313 2 0.2375537978161009 3 0.2460228636781363 4 0.40378546246349023 5 0.2414733654877867 6 0.017148108563625433 7 0.017148108563625433 8 0.01567827068674325 9 0.01700812400392237 10 0.019667830638280596 11 0.01973782291813213 12 0.02596713582491851 13 0.02645708178387924 14 0.024217328828630202 15 0.04080549915344338 16 0.019597838358429067 17 0.03933566127656119 18 0.030516634015268114 19 0.026317097224176172 20 0.027926919660761416 21 0.033106348369774816 22 0.04206536019077096 23 0.02771694282120682 24 0.046894827500526697 25 0.03569606272428151 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1428729.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.44580141345895 #Duplication Level Percentage of deduplicated Percentage of total 1 86.79978368498827 68.09078593683785 2 9.585360101858695 15.038625100537997 3 2.019184899936803 4.7518973303249235 4 0.6773171573985017 2.1253074889284553 5 0.2966928997559589 1.1637156147519618 6 0.15325837733691777 0.7213485740112485 7 0.10427453694856928 0.5725929732563498 8 0.07177872267373368 0.4504591539660365 9 0.04742997601220395 0.3348614231368625 >10 0.21952062348563764 3.0316577488246352 >50 0.014284791128909477 0.7741074547446863 >100 0.009307095156095218 1.5109309959057349 >500 0.0010842799918818905 0.5681821823039545 >1k 7.228533279212603E-4 0.8655280224692599 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1814 0.12696599565067973 No Hit GTACATGGGAAGCAGTGGTATCAAC 1729 0.12101665186329948 No Hit GTATCAACGCAGAGTACTTTTTTTT 1703 0.11919685258715963 No Hit CCCATGTACTCTGCGTTGATACCAC 1579 0.11051780988556963 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1508 0.10554835801611083 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 6.999227985153237E-5 0.0 0.0 0.0 5 0.0 6.999227985153237E-5 0.0 0.0 0.0 6 0.0 6.999227985153237E-5 0.0 0.0 0.0 7 0.0 6.999227985153237E-5 0.0 0.0 0.0 8 0.0 6.999227985153237E-5 0.0 0.0 0.0 9 0.0 6.999227985153237E-5 0.0 0.0 0.0 10 0.0 6.999227985153237E-5 0.0 0.0 0.0 11 0.0 6.999227985153237E-5 0.0 0.0 0.0 12 0.0 6.999227985153237E-5 0.0 0.0 0.0 13 0.0 6.999227985153237E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCGGA 40 0.0051067215 14.32136 1 CGCTCGC 40 0.0053130924 14.236108 18 TAGGACC 525 0.0 13.607753 4 ACCGTCT 60 4.1327838E-4 12.65166 8 CGGTAGG 55 0.0029555436 12.151457 1 CTGTCCG 65 8.0933835E-4 11.68009 9 TGGCGAG 1075 0.0 11.477171 18 GGCGAGG 480 0.0 11.467976 19 AGGACCT 905 0.0 11.453695 5 AGGGCCG 60 0.0058307066 11.094672 5 TTAGGAC 730 0.0 10.960765 3 GGACCTG 915 0.0 10.887961 6 ACTCCGT 70 0.0015069734 10.843521 6 GTCCTAA 515 0.0 10.752606 1 TGTAGGA 1135 0.0 10.748754 2 GTAGGAC 1120 0.0 10.716105 3 CTACACT 160 5.18412E-10 10.716105 4 TAGTACC 80 3.6782655E-4 10.716105 4 GGCGAGA 650 0.0 10.658829 19 GTCCTAC 1060 0.0 10.628429 1 >>END_MODULE