FastQCFastQC Report
Thu 26 May 2016
SRR1513198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428729
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25980.1818399430542811No Hit
CCCATGTACTCTGCGTTGATACCAC19560.13690489938959732No Hit
GTCCTACAGTGGACATTTCTAAATT19190.13431518503509063No Hit
TATCAACGCAGAGTACTTTTTTTTT19160.13410520819553604No Hit
GTACATGGGAAGCAGTGGTATCAAC18960.13270536259850538No Hit
GGTATCAACGCAGAGTACTTTTTTT16640.11646715367294987No Hit
CTGTAGGACGTGGAATATGGCAAGA15730.11009785620646044No Hit
GTCCTAAAGTGTGTATTTCTCATTT15440.10806808009076599No Hit
CTTTAGGACGTGAAATATGGCGAGG15030.10519839661685315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG3950.011.5296119
GTCCTAC11400.011.1955561
TAGGACC5200.011.1683534
GTCCTAG2300.010.7669141
TAGAACA3200.010.7106344
GTCCTAA5250.010.7038111
GTTCAGG2500.010.6675271
TATACAC3500.010.6093733
TAGACTG2001.8189894E-1210.472625
TAGCACC1002.3414415E-510.472624
TGGCGAG8450.010.44523718
TGTAGGA11700.010.33610252
CTAGACT1855.820766E-1110.29250054
CTTATAC1951.4551915E-1110.2572371
ACTGTTC1951.6370905E-1110.2184618
ATAGGAC2151.8189894E-1210.1855043
CTATACT2250.010.1552684
AAAGGCG1605.9499143E-910.1155995
AAGGCGT1605.9535523E-910.1152446
GGTCTAA951.6013016E-410.0258711
Produced by FastQC (version 0.11.5)