FastQCFastQC Report
Thu 26 May 2016
SRR1513197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719720
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50500.18568087891400586No Hit
GTCCTAAAGTGTGTATTTCTCATTT40470.1488020825673231No Hit
CTGTAGGACGTGGAATATGGCAAGA40050.14725780595061258No Hit
CTTTAGGACGTGAAATATGGCGAGG32380.11905637345020811No Hit
GTACATGGGAAGCAGTGGTATCAAC31290.11504860794493552No Hit
CCCATGTACTCTGCGTTGATACCAC27340.10052505404968158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9900.012.8009494
GGCGAGG10450.012.2595419
AGGACGT22250.011.7959085
GTAGGAC25150.011.55546953
GGACGTG22050.011.5304726
TGTAGGA26850.011.5055482
GTCCTAA12900.011.2525581
TAGGACT6300.011.1922384
GTCGTAC600.005652891511.1415131
CTGTAGG26600.011.0577431
TAGGACG24200.011.024744
GTCCTAC26450.010.9760311
GACGTGG11300.010.9154517
GGACCTG16850.010.8662156
AGGACCT18300.010.8084785
TTAGGAC16650.010.7589363
TGGCGAG24750.010.65904418
TCCTACA28550.010.3862992
GACGTGA12800.010.3775497
CGTGGAA12150.010.3093279
Produced by FastQC (version 0.11.5)