##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513197_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2719720 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.512934787404586 31.0 30.0 34.0 25.0 34.0 2 29.35953554042328 31.0 30.0 34.0 22.0 34.0 3 29.564780565646462 31.0 30.0 34.0 23.0 34.0 4 32.63974673863486 37.0 33.0 37.0 23.0 37.0 5 32.796468386451544 37.0 33.0 37.0 25.0 37.0 6 33.14830938478961 37.0 35.0 37.0 27.0 37.0 7 33.11844160428279 37.0 35.0 37.0 26.0 37.0 8 32.99975732796023 37.0 35.0 37.0 26.0 37.0 9 34.243423955407174 39.0 35.0 39.0 25.0 39.0 10 33.82776278440428 39.0 34.0 39.0 19.0 39.0 11 34.27679430235465 39.0 35.0 39.0 26.0 39.0 12 34.265927742561736 39.0 35.0 39.0 26.0 39.0 13 34.31102797346786 39.0 35.0 39.0 26.0 39.0 14 34.53894408247908 39.0 34.0 41.0 17.0 41.0 15 34.97618137161179 40.0 34.0 41.0 23.0 41.0 16 34.82466577441795 40.0 34.0 41.0 19.0 41.0 17 34.44337284720486 39.0 34.0 41.0 17.0 41.0 18 34.185593369905725 39.0 33.0 41.0 17.0 41.0 19 34.809845498801344 39.0 34.0 41.0 20.0 41.0 20 35.02465106702161 40.0 35.0 41.0 24.0 41.0 21 35.061513685232306 40.0 35.0 41.0 24.0 41.0 22 34.7480773756122 40.0 35.0 41.0 18.0 41.0 23 34.28777484446928 39.0 34.0 41.0 11.0 41.0 24 34.53915660435633 40.0 34.0 41.0 18.0 41.0 25 34.1815749415381 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9490769978169666 1101 2 1.266275372482017 1101 3 1.168276828477996 1101 4 1.5513748656094464 1101 5 1.3657785785869265 1101 6 1.0816201228241056 1101 7 1.0304844031848006 1101 8 1.185649893895345 1101 9 1.5870427474866773 1101 10 1.749690578879374 1101 11 1.4869629982963488 1101 12 1.3692924690221133 1101 13 1.2198675624011273 1101 14 2.213261587798776 1101 15 1.8380056755210035 1101 16 1.8765634132014668 1101 17 2.193958090468911 1101 18 2.148821267713899 1101 19 1.7920237631442149 1101 20 1.4672214781557713 1101 21 1.4840875811428873 1101 22 1.8013303103458398 1101 23 1.9943070633501776 1101 24 1.5928701989835545 1101 25 1.871586758106858 1102 1 0.7722394733079163 1102 2 0.974719665963601 1102 3 0.9159977742815144 1102 4 1.291536460266343 1102 5 1.1763236319529469 1102 6 0.9484874470106561 1102 7 0.9941701117133377 1102 8 1.0129180171416863 1102 9 1.267125688210605 1102 10 1.5143891609859708 1102 11 1.1647847973986885 1102 12 1.0260742676940637 1102 13 1.0559116246454323 1102 14 1.9991620559955834 1102 15 1.6710813245431169 1102 16 1.7263814507853468 1102 17 2.032516087840591 1102 18 2.317204118006835 1102 19 1.694462465457029 1102 20 1.593496755672298 1102 21 1.4599238232827503 1102 22 1.6299063572349652 1102 23 1.7910703772230008 1102 24 1.612278932397004 1102 25 1.8671300727915536 1103 1 0.7703183442460748 1103 2 1.109661576556995 1103 3 0.9168191208927929 1103 4 1.375633772117851 1103 5 1.1790174249820282 1103 6 0.964387535542059 1103 7 0.8794216294805111 1103 8 0.9956906194883715 1103 9 1.2367589713007163 1103 10 1.4414977031832876 1103 11 1.1245285834713883 1103 12 1.096180223753798 1103 13 1.0371573130120026 1103 14 2.01106938542339 1103 15 1.6384163535649492 1103 16 1.6733439667140217 1103 17 1.8617664377057253 1103 18 2.0841308747582517 1103 19 1.541544804476949 1103 20 1.4043240043771803 1103 21 1.4400552868816092 1103 22 1.5757864214936532 1103 23 1.973571921347407 1103 24 1.6647646597770844 1103 25 1.8659132473957953 1104 1 0.5697985630664952 1104 2 0.8522409696144493 1104 3 0.715126765326147 1104 4 0.9980559800754207 1104 5 0.9910470159489932 1104 6 0.7660652188958608 1104 7 0.6841260580534438 1104 8 0.6538746625549194 1104 9 1.0215693224266147 1104 10 1.162694270246277 1104 11 0.8009986535101561 1104 12 0.6898863255001828 1104 13 0.7432297149705036 1104 14 1.6998241264573295 1104 15 1.1949826949675 1104 16 1.25477032829086 1104 17 1.6385014193579437 1104 18 1.7862289109968188 1104 19 1.2034966597752543 1104 20 0.9849066535835149 1104 21 1.0045386701495786 1104 22 1.2654185488292171 1104 23 1.5664314243863302 1104 24 1.2769164991404054 1104 25 1.4911001452299928 1105 1 0.7662747994078885 1105 2 1.0212806420172598 1105 3 0.8546877755171955 1105 4 1.280729642182493 1105 5 1.1563279951511234 1105 6 0.8354436157873977 1105 7 0.7866383743558316 1105 8 0.8990670421512874 1105 9 1.1205089388938205 1105 10 1.5130320674602658 1105 11 0.9606684240545249 1105 12 0.8923203319552115 1105 13 0.8875089383330348 1105 14 1.6432330875207697 1105 15 1.2588387221037465 1105 16 1.5159589150344246 1105 17 1.8762270238064644 1105 18 1.5367946383242028 1105 19 1.3473496957026612 1105 20 1.1724712412603324 1105 21 1.1308294814551516 1105 22 1.2218424390159583 1105 23 1.5875020378533478 1105 24 1.486725749752857 1105 25 1.7078859118618581 1106 1 0.6942695351466703 1106 2 0.8446977331472318 1106 3 0.7748161426111757 1106 4 1.2117791924658903 1106 5 1.168812153351233 1106 6 0.7884257199653604 1106 7 0.7672807949533649 1106 8 0.7721565148911225 1106 9 1.0095909172811375 1106 10 0.792027884623451 1106 11 0.8412796181832434 1106 12 0.8098537877485228 1106 13 0.6966160606930174 1106 14 1.6972888537235846 1106 15 1.3026470276838253 1106 16 1.4225073662570153 1106 17 1.7185233289023998 1106 18 1.7362570994960862 1106 19 1.2373429278049812 1106 20 1.1299686786726184 1106 21 1.0637334498591997 1106 22 1.2219253124362126 1106 23 1.6407656993154873 1106 24 1.161942869266234 1106 25 1.567699534553391 1107 1 0.7056532254817753 1107 2 0.864939314302589 1107 3 0.9042078992316469 1107 4 1.178509888568854 1107 5 1.1200977758135906 1107 6 0.8610766861968102 1107 7 0.8187082976033793 1107 8 0.8780952455897761 1107 9 1.164598480038542 1107 10 1.3811612175671257 1107 11 1.0083859724214435 1107 12 0.9839568138179189 1107 13 0.9397123716866105 1107 14 1.827319207183315 1107 15 1.2557812534478003 1107 16 1.3377287892220693 1107 17 1.7150110752215255 1107 18 1.912363762934369 1107 19 1.329897629396811 1107 20 1.233095847174738 1107 21 1.0283125346813549 1107 22 1.402977209612807 1107 23 1.672517648511537 1107 24 1.4618676438895832 1107 25 1.7698572453454702 1108 1 0.6443627674372081 1108 2 0.8399454357963272 1108 3 0.7000903913276879 1108 4 1.1330811598319173 1108 5 1.0030993665211696 1108 6 0.6669661513120815 1108 7 0.6050955843533572 1108 8 0.5634899042149968 1108 9 0.8394722386140998 1108 10 1.0870140815766547 1108 11 0.7477649605909491 1108 12 0.5596670903525975 1108 13 0.585293653332208 1108 14 1.5826036800577796 1108 15 1.0718920930292057 1108 16 1.116521673276118 1108 17 1.4952119738664678 1108 18 1.6650948526614044 1108 19 1.1648881273724427 1108 20 0.9114947564947471 1108 21 0.9162359825111963 1108 22 1.1379105357634387 1108 23 1.3659249432408984 1108 24 1.17571740342526 1108 25 1.4400208774616843 1109 1 0.42317804224986233 1109 2 0.5968974259046149 1109 3 0.574840855354271 1109 4 0.8129340821694697 1109 5 0.970836299425379 1109 6 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1110 19 0.6027551101295572 1110 20 0.42376628110018544 1110 21 0.31815881460396866 1110 22 0.5301760901146082 1110 23 -0.5707061119728678 1110 24 0.1331535376117472 1110 25 0.6393662226510557 1111 1 -0.05052284669536533 1111 2 0.3233690353198284 1111 3 0.15174811957436418 1111 4 0.5660883772684215 1111 5 0.34635255450250924 1111 6 0.13354561119227526 1111 7 0.1289554603565506 1111 8 0.15675425488549877 1111 9 0.3194968130675875 1111 10 0.5928663625412653 1111 11 0.19811877649422627 1111 12 0.1340498487660895 1111 13 -0.14931266756120465 1111 14 0.8820011153403726 1111 15 0.43517599632117765 1111 16 0.5103685233857362 1111 17 0.8539293726126047 1111 18 1.1418518737172718 1111 19 0.6351286185528124 1111 20 0.17399864965485534 1111 21 0.1482230679688854 1111 22 0.4298676115026936 1111 23 0.7847990385807435 1111 24 0.4067680357295913 1111 25 0.5540113376345843 1112 1 0.05520987469607164 1112 2 0.2040093344363889 1112 3 0.06895764703116924 1112 4 0.5076680483560239 1112 5 0.1795897187735065 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-8.987195152790822 2215 17 -4.303363998587695 2215 18 -2.9035956580621587 2215 19 -3.4164344769327 2215 20 -3.3281148903996716 2215 21 -3.363411071919895 2215 22 -9.310813715464008 2215 23 -10.531549012984211 2215 24 -6.607474933447886 2215 25 -5.050590509953096 2216 1 -2.0409727916493843 2216 2 -2.086775436760867 2216 3 -1.8902117361425113 2216 4 -7.025164234556467 2216 5 -3.6499596133941985 2216 6 -2.8434917116735363 2216 7 -2.350245811021626 2216 8 -8.257791508861327 2216 9 -4.125106144796135 2216 10 -2.5434810904757192 2216 11 -2.470845334719538 2216 12 -2.7324429117177544 2216 13 -2.48382237047203 2216 14 -1.865134653806635 2216 15 -2.4434020173095377 2216 16 -2.184984641086885 2216 17 -1.8161198455554484 2216 18 -7.879826695544697 2216 19 -5.043162976965139 2216 20 -4.458780113237687 2216 21 -3.6664179695807277 2216 22 -3.1838502248869247 2216 23 -2.5016726781278784 2216 24 -2.9314800202571973 2216 25 -2.332772853278996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 119827.0 3 4.0 4 1003.0 5 6108.0 6 7331.0 7 6449.0 8 6069.0 9 5723.0 10 5478.0 11 5180.0 12 5004.0 13 5170.0 14 4983.0 15 5257.0 16 5200.0 17 5572.0 18 6345.0 19 7769.0 20 10070.0 21 12953.0 22 15796.0 23 19102.0 24 21844.0 25 24868.0 26 28214.0 27 31759.0 28 35797.0 29 42922.0 30 51140.0 31 61511.0 32 78271.0 33 102330.0 34 134118.0 35 180130.0 36 256598.0 37 374810.0 38 1029015.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.616471132497175 18.341696882012084 12.278025868559196 25.76380611693154 2 14.284071245993907 19.766236523782947 41.84880095490098 24.100891275322166 3 18.73896803476553 24.15644635423502 29.446075532741617 27.65851007825783 4 11.380981351028192 16.413769513971307 38.88018995082422 33.32505918417628 5 12.670529947801468 37.98423722315609 36.275988675572854 13.069244153469581 6 32.419346237935265 36.96302384721893 17.87943814047224 12.738191774373572 7 28.625746037711842 31.1344995346056 21.965512638096733 18.27424178958583 8 25.071233243455147 37.141903093368875 20.438498166396737 17.34836549677924 9 26.53872009791086 14.90202294197428 20.017299154753886 38.54195780536097 10 13.91757563008367 28.73959728927305 35.70985939216431 21.632967688478967 11 34.64697557897152 22.11444681611632 24.577291576870856 18.661286028041303 12 23.511673470168372 24.39484755656886 31.719568964851412 20.373910008411357 13 29.129848244227418 21.60314883481784 25.54301732933084 23.7239855916239 14 20.188898316273935 21.71617147150692 28.506941264694124 29.58798894752502 15 23.002088206397335 30.89898076253191 23.111776976466942 22.98715405460382 16 22.033448884828776 27.805212983251288 28.143041130006836 22.01829700191311 17 21.489816412184258 28.405901588691197 28.356537764061517 21.74774423506303 18 21.419878117093365 27.167331349888684 30.155187563515163 21.25760296950279 19 23.55489288583021 26.85460071615407 27.362514012669514 22.227992385346205 20 23.80503672057058 27.16752142033284 27.7398091589577 21.28763270013888 21 23.05556730512823 26.71868764425155 26.864304696048773 23.361440354571453 22 23.187838030212905 27.93787178035591 27.01950004175086 21.854790147680326 23 22.043211656495874 27.609331140286354 27.90882770065497 22.438629502562804 24 23.207350189780335 26.846284182715902 28.018552048516664 21.9278135789871 25 22.733487254768175 27.067759884032938 27.84132727995666 22.357425581242232 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 189.0 1 189.0 2 338.0 3 487.0 4 487.0 5 487.0 6 975.5 7 1464.0 8 1464.0 9 1464.0 10 2432.5 11 3401.0 12 3401.0 13 3401.0 14 6455.0 15 9509.0 16 9509.0 17 9509.0 18 18746.0 19 27983.0 20 27983.0 21 27983.0 22 45896.5 23 63810.0 24 63810.0 25 63810.0 26 93171.0 27 122532.0 28 122532.0 29 122532.0 30 159777.0 31 197022.0 32 197022.0 33 197022.0 34 228748.5 35 260475.0 36 260475.0 37 260475.0 38 284303.0 39 308131.0 40 308131.0 41 308131.0 42 325198.0 43 342265.0 44 342265.0 45 342265.0 46 354670.5 47 367076.0 48 367076.0 49 367076.0 50 352070.5 51 337065.0 52 337065.0 53 337065.0 54 299188.0 55 261311.0 56 261311.0 57 261311.0 58 224005.0 59 186699.0 60 186699.0 61 186699.0 62 151921.0 63 117143.0 64 117143.0 65 117143.0 66 90512.5 67 63882.0 68 63882.0 69 63882.0 70 46833.5 71 29785.0 72 29785.0 73 29785.0 74 21022.5 75 12260.0 76 12260.0 77 12260.0 78 8526.5 79 4793.0 80 4793.0 81 4793.0 82 3245.0 83 1697.0 84 1697.0 85 1697.0 86 1125.5 87 554.0 88 554.0 89 554.0 90 352.0 91 150.0 92 150.0 93 150.0 94 88.0 95 26.0 96 26.0 97 26.0 98 18.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17516509052402454 2 0.2565705293191946 3 0.2628211727677849 4 0.4593487564896386 5 0.25737943611842395 6 0.019671142617622404 7 0.019156383745385554 8 0.017795949583045312 9 0.01882546732751901 10 0.021950789051814155 11 0.021619872633947615 12 0.02856911740914506 13 0.027429294192049182 14 0.025407027193975848 15 0.04114394128807377 16 0.021546335652199492 17 0.041548394687688436 18 0.03239304046004736 19 0.027649905137293547 20 0.029708940626240934 21 0.034746223875987234 22 0.04460017943023546 23 0.03029723648022591 24 0.04831379700851558 25 0.03739355521891959 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2719720.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.50432505415448 #Duplication Level Percentage of deduplicated Percentage of total 1 82.93878132982653 59.30481579803355 2 11.607727319779169 16.60005414826958 3 2.874490157203516 6.166154356969435 4 1.0471229224524252 2.9949527127477764 5 0.5036675574683814 1.800720437422559 6 0.2733893908454882 1.1729114321623844 7 0.16250522994335137 0.813387874940866 8 0.10910878006214864 0.62413997406609 9 0.0773147149969915 0.49755028613527735 >10 0.3683094075164563 4.557173836155202 >50 0.021585177398906624 1.0638383487989092 >100 0.012881532821629776 1.751109463945584 >500 0.002181535779568568 1.0959729967551832 >1k 8.83002577398619E-4 1.3696598384470522 >5k 5.19413280822717E-5 0.18755849515043152 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5050 0.18568087891400586 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4047 0.1488020825673231 No Hit CTGTAGGACGTGGAATATGGCAAGA 4005 0.14725780595061258 No Hit CTTTAGGACGTGAAATATGGCGAGG 3238 0.11905637345020811 No Hit GTACATGGGAAGCAGTGGTATCAAC 3129 0.11504860794493552 No Hit CCCATGTACTCTGCGTTGATACCAC 2734 0.10052505404968158 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.676849087406057E-5 0.0 0.0 0.0 6 0.0 3.676849087406057E-5 0.0 0.0 0.0 7 0.0 7.353698174812113E-5 0.0 0.0 0.0 8 0.0 7.353698174812113E-5 0.0 0.0 0.0 9 0.0 7.353698174812113E-5 0.0 0.0 0.0 10 0.0 7.353698174812113E-5 0.0 0.0 0.0 11 0.0 7.353698174812113E-5 0.0 0.0 0.0 12 0.0 7.353698174812113E-5 0.0 0.0 0.0 13 0.0 7.353698174812113E-5 0.0 0.0 3.676849087406057E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 990 0.0 12.800949 4 GGCGAGG 1045 0.0 12.25954 19 AGGACGT 2225 0.0 11.795908 5 GTAGGAC 2515 0.0 11.5554695 3 GGACGTG 2205 0.0 11.530472 6 TGTAGGA 2685 0.0 11.505548 2 GTCCTAA 1290 0.0 11.252558 1 TAGGACT 630 0.0 11.192238 4 GTCGTAC 60 0.0056528915 11.141513 1 CTGTAGG 2660 0.0 11.057743 1 TAGGACG 2420 0.0 11.02474 4 GTCCTAC 2645 0.0 10.976031 1 GACGTGG 1130 0.0 10.915451 7 GGACCTG 1685 0.0 10.866215 6 AGGACCT 1830 0.0 10.808478 5 TTAGGAC 1665 0.0 10.758936 3 TGGCGAG 2475 0.0 10.659044 18 TCCTACA 2855 0.0 10.386299 2 GACGTGA 1280 0.0 10.377549 7 CGTGGAA 1215 0.0 10.309327 9 >>END_MODULE