FastQCFastQC Report
Thu 26 May 2016
SRR1513197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719720
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT49900.18347476946156221No Hit
CTGTAGGACGTGGAATATGGCAAGA43670.1605679996470225No Hit
CTTTAGGACGTGAAATATGGCGAGG40800.1500154427661671No Hit
GTCCTAAAGTGTGTATTTCTCATTT40440.14869177709470094No Hit
GTATCAACGCAGAGTACTTTTTTTT37000.13604341623402408No Hit
GTACATGGGAAGCAGTGGTATCAAC36530.13431529716294324No Hit
CCCATGTACTCTGCGTTGATACCAC33710.12394658273645817No Hit
GTCCTACAGTGTGCATTTCTCATTT28330.10416513464621359No Hit
TATCAACGCAGAGTACTTTTTTTTT27360.10059859103142971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC707.3486754E-613.556818
TAGGACC9800.012.62840754
TCCAACG1551.8189894E-1212.24598718
CCTACGC600.005786048711.1067263
TGGCGAG26050.010.92971918
GGCGAGG11350.010.86773819
TATACCG803.700466E-410.709865
GTAGGAC26050.010.7077743
CGTCCAA803.8149237E-410.67461310
AGGACGT23600.010.5686765
GTCCTAC25900.010.5179221
TTTAGAA8700.010.5056612
GGACGTG22850.010.4565116
TGTAGGA28350.010.4107022
AGGACCT19850.010.3591595
GGCGAGA16150.010.34026819
GTCTTAT2500.010.2869691
GGACCTG18350.010.1157436
CTGTAGG28900.010.0852631
ATTTAGA7850.010.070991
Produced by FastQC (version 0.11.5)