##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513197_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2719720 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.774193299310223 31.0 31.0 34.0 27.0 34.0 2 30.884799538187753 33.0 31.0 34.0 27.0 34.0 3 30.994582898239525 33.0 31.0 34.0 27.0 34.0 4 34.38752665715588 37.0 35.0 37.0 31.0 37.0 5 34.13630484020414 37.0 35.0 37.0 30.0 37.0 6 34.05835674260586 37.0 35.0 37.0 30.0 37.0 7 33.927385172003 37.0 35.0 37.0 30.0 37.0 8 33.89094796523172 37.0 35.0 37.0 29.0 37.0 9 35.447256702895885 39.0 35.0 39.0 30.0 39.0 10 35.29729016222258 39.0 35.0 39.0 29.0 39.0 11 35.31135668377627 39.0 35.0 39.0 29.0 39.0 12 35.24593414027915 39.0 35.0 39.0 29.0 39.0 13 35.216506110923184 39.0 35.0 39.0 29.0 39.0 14 36.38189372435398 40.0 37.0 41.0 28.0 41.0 15 36.36818165105232 40.0 37.0 41.0 28.0 41.0 16 36.33637286191225 40.0 37.0 41.0 28.0 41.0 17 36.27495661318077 40.0 36.0 41.0 28.0 41.0 18 36.23093296368744 40.0 36.0 41.0 27.0 41.0 19 36.303460282676156 40.0 37.0 41.0 28.0 41.0 20 36.27207322812642 40.0 37.0 41.0 28.0 41.0 21 36.2154762254938 40.0 37.0 41.0 27.0 41.0 22 36.15698086567735 40.0 37.0 41.0 27.0 41.0 23 36.079061815186854 40.0 37.0 41.0 27.0 41.0 24 36.025711102613506 40.0 37.0 41.0 27.0 41.0 25 35.968780242083746 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5366675767332012 1101 2 0.6083951788501203 1101 3 0.5771519069894708 1101 4 0.6172624424807438 1101 5 0.5726266605435697 1101 6 0.596636497003928 1101 7 0.6161731637597399 1101 8 0.649889689510573 1101 9 0.6077353017305782 1101 10 0.7972056852146636 1101 11 0.7126546024685751 1101 12 0.7674963365300016 1101 13 0.7202031073316064 1101 14 0.8576625965347517 1101 15 0.9264281244074226 1101 16 0.8499460303521857 1101 17 0.8188909276850893 1101 18 0.8112569705249939 1101 19 0.8340376514346488 1101 20 0.8535954994852162 1101 21 0.8472235126378678 1101 22 0.7485626927011424 1101 23 0.8547425776138553 1101 24 0.938248663690338 1101 25 0.9375806690448414 1102 1 0.38008079892787094 1102 2 0.5185870916098878 1102 3 0.4759837625659422 1102 4 0.4931339228737528 1102 5 0.48988804915082795 1102 6 0.4421479358642131 1102 7 0.44761846279092055 1102 8 0.5309454236620397 1102 9 0.4741102440802081 1102 10 0.567683670965792 1102 11 0.589165311131616 1102 12 0.5285038277766887 1102 13 0.5663387963702817 1102 14 0.6490697448381582 1102 15 0.5482915897484872 1102 16 0.5927482319971631 1102 17 0.6726070594688238 1102 18 0.6601687418622291 1102 19 0.6739343233882735 1102 20 0.6850952698619039 1102 21 0.600265226806485 1102 22 0.6618894283379504 1102 23 0.6303330445537227 1102 24 0.6840266147656564 1102 25 0.7054570519812131 1103 1 0.4375485239596344 1103 2 0.38388378013099 1103 3 0.395043120007994 1103 4 0.5421120326536553 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2214 10 -0.7252997982600391 2214 11 -0.7030682102702457 2214 12 -0.807027870150101 2214 13 -0.7733109549900377 2214 14 -0.9140606054060783 2214 15 -0.7866582464143903 2214 16 -0.8850390905933381 2214 17 -0.8124289882213844 2214 18 -0.8575336813069185 2214 19 -0.8545187829125638 2214 20 -0.8541146838269214 2214 21 -0.8803274928545903 2214 22 -0.8861523635585158 2214 23 -0.944711602274019 2214 24 -0.8606002892190077 2214 25 -0.8493566157600867 2215 1 -0.4056367541105459 2215 2 -0.45289396442919383 2215 3 -0.4773172547743023 2215 4 -0.29021009463548353 2215 5 -0.435101939892796 2215 6 -0.5364893164219282 2215 7 -0.6750066370457191 2215 8 -0.6354843789005074 2215 9 -0.7823773905873495 2215 10 -0.8029712246211389 2215 11 -0.7177074014685658 2215 12 -0.7695828240922538 2215 13 -0.7771078045115516 2215 14 -0.9954402117511947 2215 15 -0.9812729369243272 2215 16 -0.9115813701715823 2215 17 -0.8575783211724115 2215 18 -0.8082846276051612 2215 19 -0.883089153996572 2215 20 -0.8688481735839417 2215 21 -0.918154714581938 2215 22 -1.0225782180414456 2215 23 -0.8691253048459018 2215 24 -0.9312602466237578 2215 25 -1.0330267455533573 2216 1 -0.560831788640769 2216 2 -0.5103023497950154 2216 3 -0.5298109889155356 2216 4 -0.3572608570709548 2216 5 -0.5786714924732763 2216 6 -0.7373784124128733 2216 7 -0.9353026098517887 2216 8 -0.7993225685939436 2216 9 -0.9530513613806875 2216 10 -0.8582252997516093 2216 11 -0.901535507480979 2216 12 -0.9926610677977905 2216 13 -0.9700923482978681 2216 14 -1.1937311211850883 2216 15 -1.1112292343041261 2216 16 -1.1852985757000383 2216 17 -1.3017008756249098 2216 18 -1.2359202587836648 2216 19 -1.2882300020794943 2216 20 -1.2880608728483551 2216 21 -1.508208731443517 2216 22 -1.347883017402964 2216 23 -1.3237804184697595 2216 24 -1.271024324310396 2216 25 -1.3998061613153254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63582.0 3 0.0 4 1102.0 5 8729.0 6 9774.0 7 7462.0 8 6173.0 9 5373.0 10 4719.0 11 4384.0 12 4073.0 13 3930.0 14 3822.0 15 3871.0 16 3962.0 17 4127.0 18 4579.0 19 5535.0 20 6938.0 21 9284.0 22 11481.0 23 14117.0 24 16411.0 25 18409.0 26 20386.0 27 22177.0 28 25578.0 29 30516.0 30 38436.0 31 49721.0 32 63352.0 33 82685.0 34 110181.0 35 148131.0 36 211978.0 37 346718.0 38 1348024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.784924847468154 19.290641729648332 13.300725426468471 27.62370799641505 2 13.680041419348102 20.126883524998327 40.15191016590538 26.0411648897482 3 17.11103233938781 24.185153916188522 29.82020435869113 28.883609385732544 4 11.253368082928379 16.848283307251528 37.451979079645604 34.44636953017449 5 12.641811596922775 38.20289426291824 36.05729354108203 13.09800059907695 6 32.457231227134436 37.408016657068835 17.481806232455977 12.652945883340754 7 28.311132230499158 31.25074017979105 22.025139264138076 18.412988325571714 8 25.163459742200633 37.42183719189034 20.034868960079784 17.379834105829243 9 26.255281126934577 14.794141764015901 19.685614375696336 39.26496273335319 10 14.370947032150175 29.115329686734725 34.84555885177738 21.668164429337715 11 34.664083067374584 22.02171547071022 24.50803759210507 18.806163869810128 12 23.217390025443795 24.5431515008898 31.775035665436146 20.46442280823026 13 29.019200505934435 21.491403526833643 25.68065830306061 23.80873766417131 14 20.646575830033008 21.821897494595873 27.447373423574312 30.084153251796806 15 23.004757842719105 31.056873501683995 22.9344564881679 23.003912167429 16 22.21361022458194 27.97089406262409 27.616335505125527 22.199160207668438 17 21.74523838506498 28.703736733655905 27.608669189719155 21.94235569155996 18 21.74196723264995 27.471582529278106 29.39888764902024 21.387562589051708 19 23.480873031047313 26.942074919477005 27.349102113452854 22.227949936022824 20 23.669274778286002 27.17515038312768 27.789809245069346 21.36576559351698 21 22.836591228226926 26.709222815302127 27.0608423499495 23.39334360652145 22 23.13547833434263 27.93288571356085 26.823469630502267 22.108166321594254 23 22.089996117053197 27.67327650111194 27.48611552355066 22.75061185828421 24 23.116350212521876 26.91015986939832 27.97637256776433 21.997117350315474 25 22.75514162329509 27.12843073630877 27.633373349781138 22.48305429061501 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 97.0 1 97.0 2 250.0 3 403.0 4 403.0 5 403.0 6 989.0 7 1575.0 8 1575.0 9 1575.0 10 2464.5 11 3354.0 12 3354.0 13 3354.0 14 6202.0 15 9050.0 16 9050.0 17 9050.0 18 18076.5 19 27103.0 20 27103.0 21 27103.0 22 44734.5 23 62366.0 24 62366.0 25 62366.0 26 91610.5 27 120855.0 28 120855.0 29 120855.0 30 158249.5 31 195644.0 32 195644.0 33 195644.0 34 225545.0 35 255446.0 36 255446.0 37 255446.0 38 280265.5 39 305085.0 40 305085.0 41 305085.0 42 322080.5 43 339076.0 44 339076.0 45 339076.0 46 353441.5 47 367807.0 48 367807.0 49 367807.0 50 354389.0 51 340971.0 52 340971.0 53 340971.0 54 302680.5 55 264390.0 56 264390.0 57 264390.0 58 227830.0 59 191270.0 60 191270.0 61 191270.0 62 155665.0 63 120060.0 64 120060.0 65 120060.0 66 92511.0 67 64962.0 68 64962.0 69 64962.0 70 47665.5 71 30369.0 72 30369.0 73 30369.0 74 21475.5 75 12582.0 76 12582.0 77 12582.0 78 8715.0 79 4848.0 80 4848.0 81 4848.0 82 3271.5 83 1695.0 84 1695.0 85 1695.0 86 1127.0 87 559.0 88 559.0 89 559.0 90 349.0 91 139.0 92 139.0 93 139.0 94 76.0 95 13.0 96 13.0 97 13.0 98 7.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18534996249613933 2 0.008015531010545203 3 0.006912476284323387 4 0.0026473313429323606 5 0.08111129086817762 6 0.3323503890106334 7 0.028900033827011604 8 0.014560322386127984 9 0.004779903813627874 10 0.003970997014398541 11 0.0 12 0.0 13 0.0 14 0.001433971144088362 15 0.0 16 0.0 17 0.01937699469062992 18 0.029194181754004088 19 0.0 20 0.0 21 6.986013266071508E-4 22 3.676849087406057E-5 23 0.005000514758872237 24 0.0 25 1.8384245437030284E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2719720.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.75448259054622 #Duplication Level Percentage of deduplicated Percentage of total 1 81.28968765874754 55.89030414924297 2 12.392336957380845 17.040574311848477 3 3.356592791765531 6.923424019949882 4 1.2315501607647197 3.3869837635072924 5 0.5484818127393661 1.8855291622609989 6 0.3130729790173691 1.2915102411252084 7 0.18757885322596643 0.9027820898935532 8 0.11462788010709524 0.6304944469771598 9 0.09002859115313844 0.5570882282780873 >10 0.4325897340127047 5.120532977309783 >50 0.024473919731989143 1.1796741426327502 >100 0.015665070993885415 2.1029455619434536 >500 0.0020309102206247215 1.058904790929351 >1k 0.001282680139270286 2.0292521141011073 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4990 0.18347476946156221 No Hit CTGTAGGACGTGGAATATGGCAAGA 4367 0.1605679996470225 No Hit CTTTAGGACGTGAAATATGGCGAGG 4080 0.1500154427661671 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4044 0.14869177709470094 No Hit GTATCAACGCAGAGTACTTTTTTTT 3700 0.13604341623402408 No Hit GTACATGGGAAGCAGTGGTATCAAC 3653 0.13431529716294324 No Hit CCCATGTACTCTGCGTTGATACCAC 3371 0.12394658273645817 No Hit GTCCTACAGTGTGCATTTCTCATTT 2833 0.10416513464621359 No Hit TATCAACGCAGAGTACTTTTTTTTT 2736 0.10059859103142971 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.353698174812113E-5 2 0.0 0.0 0.0 0.0 7.353698174812113E-5 3 0.0 0.0 0.0 0.0 7.353698174812113E-5 4 0.0 0.0 0.0 0.0 7.353698174812113E-5 5 0.0 3.676849087406057E-5 0.0 0.0 7.353698174812113E-5 6 0.0 3.676849087406057E-5 0.0 0.0 7.353698174812113E-5 7 0.0 3.676849087406057E-5 0.0 0.0 7.353698174812113E-5 8 0.0 3.676849087406057E-5 0.0 0.0 7.353698174812113E-5 9 0.0 3.676849087406057E-5 0.0 0.0 7.353698174812113E-5 10 0.0 3.676849087406057E-5 0.0 0.0 7.353698174812113E-5 11 0.0 3.676849087406057E-5 0.0 0.0 7.353698174812113E-5 12 0.0 3.676849087406057E-5 0.0 0.0 7.353698174812113E-5 13 0.0 3.676849087406057E-5 0.0 0.0 7.353698174812113E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCC 70 7.3486754E-6 13.55681 8 TAGGACC 980 0.0 12.6284075 4 TCCAACG 155 1.8189894E-12 12.245987 18 CCTACGC 60 0.0057860487 11.106726 3 TGGCGAG 2605 0.0 10.929719 18 GGCGAGG 1135 0.0 10.867738 19 TATACCG 80 3.700466E-4 10.70986 5 GTAGGAC 2605 0.0 10.707774 3 CGTCCAA 80 3.8149237E-4 10.674613 10 AGGACGT 2360 0.0 10.568676 5 GTCCTAC 2590 0.0 10.517922 1 TTTAGAA 870 0.0 10.505661 2 GGACGTG 2285 0.0 10.456511 6 TGTAGGA 2835 0.0 10.410702 2 AGGACCT 1985 0.0 10.359159 5 GGCGAGA 1615 0.0 10.340268 19 GTCTTAT 250 0.0 10.286969 1 GGACCTG 1835 0.0 10.115743 6 CTGTAGG 2890 0.0 10.085263 1 ATTTAGA 785 0.0 10.07099 1 >>END_MODULE