FastQCFastQC Report
Thu 26 May 2016
SRR1513196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724857
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT35610.13068575708743616No Hit
GTACATGGGAAGCAGTGGTATCAAC35460.1301352694838665No Hit
CCCATGTACTCTGCGTTGATACCAC29660.1088497488125065No Hit
GTCCTAAAGTGTGTATTTCTCATTT29090.1067578959189418No Hit
CTGTAGGACGTGGAATATGGCAAGA28140.10327147442966732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7550.012.3670964
GTCCTAC20500.011.1805641
GGCGAGG7700.011.09212619
AATCCCG1201.2953751E-711.07158619
GTAGGAC18100.011.0540483
AGGACGT15450.011.0184735
GGACGTG15500.010.8345016
TAGGACT5400.010.5863474
TTAGGAC11250.010.586153
TGTAGGA20150.010.5036142
CTAGACT3200.010.4209354
TCCTACA22900.010.4079532
GTCCTAA10450.010.2354771
TAGGACG16850.010.177974
GTCTTAC2350.010.1596441
CTGTAGG20000.010.123071
GTCCTAT3400.010.1118351
GACGTGG7850.010.0322827
ATGGCAA15250.010.01887717
TGGCAAG15300.09.86190318
Produced by FastQC (version 0.11.5)