##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513196_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2724857 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.698784927062228 31.0 30.0 34.0 25.0 34.0 2 29.54544330216228 31.0 30.0 34.0 23.0 34.0 3 29.754617948758412 31.0 30.0 34.0 25.0 34.0 4 32.84987725961399 37.0 35.0 37.0 25.0 37.0 5 32.99264181569895 37.0 35.0 37.0 26.0 37.0 6 33.35864414169257 37.0 35.0 37.0 28.0 37.0 7 33.34439018267748 37.0 35.0 37.0 28.0 37.0 8 33.22421323394219 37.0 35.0 37.0 27.0 37.0 9 34.488481413887044 39.0 35.0 39.0 26.0 39.0 10 34.068703054875904 39.0 34.0 39.0 23.0 39.0 11 34.52098954183651 39.0 35.0 39.0 27.0 39.0 12 34.52014509385263 39.0 35.0 39.0 27.0 39.0 13 34.56073621478118 39.0 35.0 39.0 27.0 39.0 14 34.81042161111574 40.0 34.0 41.0 18.0 41.0 15 35.24608447342374 40.0 35.0 41.0 25.0 41.0 16 35.08583422909899 40.0 35.0 41.0 24.0 41.0 17 34.699012461938366 39.0 34.0 41.0 18.0 41.0 18 34.42783896549434 39.0 33.0 41.0 17.0 41.0 19 35.06400042277448 40.0 34.0 41.0 25.0 41.0 20 35.27957503824971 40.0 35.0 41.0 25.0 41.0 21 35.3283453039921 40.0 36.0 41.0 26.0 41.0 22 35.01766037630598 40.0 35.0 41.0 22.0 41.0 23 34.55495939786932 40.0 34.0 41.0 18.0 41.0 24 34.80298782651713 40.0 35.0 41.0 22.0 41.0 25 34.44208558467472 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9558409455491095 1101 2 1.2000297939834468 1101 3 1.1157401721620097 1101 4 1.5146207664709408 1101 5 1.3455392316933157 1101 6 1.0283167912333298 1101 7 1.0418428226245453 1101 8 1.0296846758601035 1101 9 1.4804970445718482 1101 10 1.8177354393650802 1101 11 1.3689098351737243 1101 12 1.2669234845585464 1101 13 1.1576286937385305 1101 14 2.176371170190258 1101 15 1.7749134109061728 1101 16 1.83906485704901 1101 17 2.233949074567647 1101 18 2.2519205381563765 1101 19 1.8210751045931275 1101 20 1.4127280855719633 1101 21 1.3830045643847697 1101 22 1.6735298070086841 1101 23 2.0096672418855945 1101 24 1.5728141050179545 1101 25 1.8818444875696656 1102 1 0.8719305136829583 1102 2 1.0954512754934846 1102 3 0.920020222567274 1102 4 1.2082758951826662 1102 5 1.220156313103118 1102 6 0.9041597301591082 1102 7 0.855908771120582 1102 8 0.8807139239253345 1102 9 1.2651021149753845 1102 10 1.5315806841149353 1102 11 1.1166991233318626 1102 12 0.9609888788857859 1102 13 0.9367692984089686 1102 14 2.095226040611138 1102 15 1.5679383533208124 1102 16 1.608997886954846 1102 17 1.8868256924995848 1102 18 2.2056634062449234 1102 19 1.5157261710066408 1102 20 1.3928123433230937 1102 21 1.2541765229618562 1102 22 1.5342886929848447 1102 23 1.786596532356782 1102 24 1.6563127051393849 1102 25 1.9031062576329845 1103 1 0.8290112170797173 1103 2 1.0808165997677754 1103 3 0.8318130711499343 1103 4 1.3291877676592208 1103 5 1.1138530921846197 1103 6 0.8738713081976286 1103 7 0.8327127424923191 1103 8 0.9512884155028374 1103 9 1.2416170685219399 1103 10 1.5100712608535645 1103 11 1.1072141816930028 1103 12 1.1958701536416498 1103 13 1.1669722653794565 1103 14 2.0170466889874206 1103 15 1.5932606461851861 1103 16 1.668465024473491 1103 17 2.093405894317904 1103 18 2.181408822162574 1103 19 1.6663423275756912 1103 20 1.2067754399764965 1103 21 1.2600795819203015 1103 22 1.5375790451618272 1103 23 1.988615086451631 1103 24 1.6515600938684187 1103 25 1.785594278140266 1104 1 0.6843805637280553 1104 2 0.9399130623508434 1104 3 0.7829881109305532 1104 4 1.1779298704379357 1104 5 1.044962435921022 1104 6 0.8095480934370443 1104 7 0.7803374625649653 1104 8 0.8056416392048789 1104 9 1.121850639310999 1104 10 1.3428389520217578 1104 11 1.0822998976037326 1104 12 0.888696903797964 1104 13 0.9208148070993118 1104 14 1.8789881665573276 1104 15 1.4138322767422977 1104 16 1.4745177422997742 1104 17 1.8532797785010402 1104 18 2.0247854620518666 1104 19 1.430038688370196 1104 20 1.2655519889353002 1104 21 1.176389101407075 1104 22 1.3919516582805613 1104 23 1.814066034341458 1104 24 1.541145591618836 1104 25 1.7485201857735504 1105 1 0.4714315208245097 1105 2 0.7146258772147824 1105 3 0.6629606943978317 1105 4 1.0891337203673146 1105 5 0.9283294193718135 1105 6 0.7281102428857125 1105 7 0.5878393546522886 1105 8 0.6795316770840927 1105 9 0.9424028993250104 1105 10 1.2530450068885415 1105 11 0.7516770730358218 1105 12 0.7533193433916736 1105 13 0.5908676778266582 1105 14 1.4521795137235074 1105 15 1.0341800367731722 1105 16 1.3400414992358733 1105 17 1.5874986461710705 1105 18 1.2410831248556846 1105 19 1.029643036049677 1105 20 0.8102954050334077 1105 21 0.9204025652007672 1105 22 1.05895412361415 1105 23 1.3564517176097866 1105 24 1.1212458675638501 1105 25 1.3805924475859825 1106 1 0.36511399547312706 1106 2 0.5880113509013185 1106 3 0.499920491586785 1106 4 0.9396853271527448 1106 5 0.8672010064732945 1106 6 0.4865792489567724 1106 7 0.5432235510936962 1106 8 0.4576048062793774 1106 9 0.6348249237861907 1106 10 0.6442065120472833 1106 11 0.5530357356329958 1106 12 0.5160631119836054 1106 13 0.4363040837362675 1106 14 1.510345657880805 1106 15 1.065470469046005 1106 16 1.0976755961516815 1106 17 1.4481876950381363 1106 18 1.5097683641041542 1106 19 1.1118898816412468 1106 20 0.8561926006233165 1106 21 0.6690292502243764 1106 22 1.0141993989130071 1106 23 1.3121680116083922 1106 24 0.9608206306376417 1106 25 1.2219102908663473 1107 1 0.7936232651471933 1107 2 0.9512715471516557 1107 3 0.8601528941810308 1107 4 1.1823343211197326 1107 5 1.1680399400542782 1107 6 0.8292079596621491 1107 7 0.833215633357689 1107 8 0.8994391730532669 1107 9 1.169793099181085 1107 10 1.3353964242816687 1107 11 0.9228613386968334 1107 12 0.9202624176590675 1107 13 0.8347406521660119 1107 14 1.7750007614120449 1107 15 1.2120677703105471 1107 16 1.3373097039927018 1107 17 1.7589180433235327 1107 18 1.8708352860158328 1107 19 1.3935049768580257 1107 20 1.1509339834500452 1107 21 0.9641999900270051 1107 22 1.284708821689378 1107 23 1.5204287191310257 1107 24 1.2972557943257996 1107 25 1.6292080184146513 1108 1 0.6735766739335531 1108 2 0.9166518015479284 1108 3 0.7457158026272346 1108 4 1.2572101122426602 1108 5 1.1257326442000704 1108 6 0.6948974477772651 1108 7 0.6807778408741285 1108 8 0.6991113853112907 1108 9 0.9203528122121938 1108 10 1.0932787917750204 1108 11 0.753823880185692 1108 12 0.5424921989277465 1108 13 0.5844406954587384 1108 14 1.5783549164824464 1108 15 1.1358890744522228 1108 16 1.2993028183885613 1108 17 1.6081769131773669 1108 18 1.813544585704534 1108 19 1.168698247932305 1108 20 0.972378311072859 1108 21 0.9750376010934545 1108 22 1.2181514176033659 1108 23 1.5260753268371303 1108 24 1.1529920798103674 1108 25 1.5665490207442119 1109 1 0.47074736467205014 1109 2 0.6404952226229668 1109 3 0.5476497010067618 1109 4 0.8588785401970327 1109 5 0.9156935956001817 1109 6 0.5500606649193855 1109 7 0.461373044076538 1109 8 0.39072436183373327 1109 9 0.7419703331258063 1109 10 1.070460032313143 1109 11 0.7027686089160099 1109 12 0.5904649926348 1109 13 0.5735096196211842 1109 14 1.4602597917088858 1109 15 0.9639555749393764 1109 16 1.1644299717084294 1109 17 1.4106896164089022 1109 18 1.5522331299845646 1109 19 0.9993753084372443 1109 20 0.8573318274187756 1109 21 0.7752304912276387 1109 22 1.0877857644166014 1109 23 1.3392786654857005 1109 24 1.0233414735778297 1109 25 1.3076821920211472 1110 1 0.3199270352654864 1110 2 0.576166458363371 1110 3 0.418233031257099 1110 4 0.749344684671108 1110 5 0.6590744969551707 1110 6 0.3236266373845993 1110 7 0.3337238222617671 1110 8 0.3587408642547345 1110 9 0.4884459677288717 1110 10 0.9939577628569509 1110 11 0.43439928550947826 1110 12 0.4164349996194545 1110 13 0.131549072901322 1110 14 1.0146922515961379 1110 15 0.7156492538475376 1110 16 0.9148418859188396 1110 17 1.2356632957984601 1110 18 0.8933941519463389 1110 19 0.6056535206326217 1110 20 0.3934184206670608 1110 21 0.1427974817499944 1110 22 0.4447275078350046 1110 23 -0.7220979447851832 1110 24 0.13920369755129514 1110 25 0.5406443884036563 1111 1 0.3814083025026562 1111 2 0.6904207097322157 1111 3 0.6510794925796439 1111 4 0.9063569438666477 1111 5 0.8321587240897443 1111 6 0.5699762648667956 1111 7 0.5231050040333116 1111 8 0.6420007461016937 1111 9 0.7751692762313098 1111 10 1.1292636117182369 1111 11 0.6897952463493056 1111 12 0.6194907349555905 1111 13 0.39834674920090407 1111 14 1.3102338656636334 1111 15 0.8964141999316482 1111 16 0.8876927307620832 1111 17 1.374824709607708 1111 18 1.5769536467814191 1111 19 0.9955841413167761 1111 20 0.6927061202755525 1111 21 0.4868758351188873 1111 22 0.904508542504459 1111 23 1.3060169208348213 1111 24 1.0014080610659377 1111 25 1.034848224940042 1112 1 0.3511757205027983 1112 2 0.48465300445380066 1112 3 0.3775506732231335 1112 4 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13 0.7322161043434292 1203 14 1.6519047175797326 1203 15 1.2837723708850461 1203 16 1.473288293785302 1203 17 1.856871040927615 1203 18 1.4498925728327663 1203 19 1.133210194057341 1203 20 1.1812939466558348 1203 21 1.0154832416048478 1203 22 1.285866404904084 1203 23 1.6284525213006162 1203 24 1.327842022699464 1203 25 1.1283216484770264 1204 1 0.20399638783297647 1204 2 0.45795151526253974 1204 3 0.40838117329952794 1204 4 0.5955871969368189 1204 5 0.3564797064106884 1204 6 0.11479073037969556 1204 7 0.1483067134225564 1204 8 0.38770732787864404 1204 9 0.47400438031340997 1204 10 0.9358452764595881 1204 11 0.30652002783664045 1204 12 0.3312627311113161 1204 13 0.17597393650156334 1204 14 0.552932577674639 1204 15 0.49944136961551777 1204 16 0.35159711537802707 1204 17 0.7688274284275352 1204 18 1.2282186610235257 1204 19 0.6712202391202382 1204 20 0.4825587395942108 1204 21 0.4050003094906742 1204 22 0.5898747724730811 1204 23 0.9375970286787236 1204 24 0.607907588947306 1204 25 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-2.200236097110512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 106538.0 3 2.0 4 936.0 5 5378.0 6 6670.0 7 5977.0 8 5572.0 9 5371.0 10 5064.0 11 4895.0 12 4702.0 13 4722.0 14 4714.0 15 5069.0 16 4975.0 17 5300.0 18 6071.0 19 7443.0 20 9833.0 21 12259.0 22 15383.0 23 18439.0 24 21771.0 25 24608.0 26 28252.0 27 31966.0 28 35691.0 29 42777.0 30 51879.0 31 61792.0 32 79564.0 33 103359.0 34 136538.0 35 183154.0 36 260529.0 37 379645.0 38 1038019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70069664852099 18.276646925412027 12.387615840013117 25.63504058605387 2 14.544165524000766 19.786472626238062 41.24763296160974 24.421728888151435 3 18.736591372929354 24.09868087256014 29.418937111400467 27.74579064311004 4 11.564457331892712 16.33871133627282 38.55754212474062 33.539289207093844 5 12.937134590599825 37.73591222705704 36.03084311958773 13.296110062755403 6 32.607509910439 36.600132139186606 17.86029951548965 12.932058434884745 7 28.73899389075597 31.223654710989152 21.781011277362026 18.25634012089285 8 25.42893281398425 36.518200464242874 20.372840470468017 17.680026251304856 9 26.542731798848035 15.039267534580244 19.916399573041726 38.50160109353 10 14.024292211295988 28.667231943266785 35.40220898349319 21.906266861944037 11 34.892603779762595 22.251060894870008 24.102841373963926 18.75349395140347 12 23.407720762465946 24.411626185089645 31.41866431536168 20.76198873708272 13 29.063167632094792 21.479968284034097 25.549009243148397 23.907854840722713 14 20.561330866590318 21.402409479341028 28.30995066513963 29.726308988929024 15 23.36772875661022 30.136698127477228 23.1126817856739 23.38289133023865 16 22.47724775046929 27.74908179798014 27.471106827106397 22.302563624444176 17 21.837988342773563 28.083481777690317 28.020041089593818 22.058488789942302 18 21.752500758973785 27.198169799414334 29.424321594905862 21.62500784670602 19 23.637935401455834 26.85083014892391 27.279394760315835 22.231839689304422 20 23.912522369568208 26.935988620199147 27.44925434772633 21.70223466250631 21 23.373814288341727 26.554801638915166 26.822129392947204 23.249254679795904 22 23.439334562524643 27.429837334079366 26.825998938225425 22.304829165170563 23 22.470769663368866 27.125891887308246 27.610008153034777 22.79333029628811 24 23.286542596313136 26.75307385475779 27.542392172758568 22.417991376170505 25 22.91062838732557 26.856675676896085 27.471556836216347 22.761139099561994 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 173.0 1 173.0 2 318.5 3 464.0 4 464.0 5 464.0 6 902.0 7 1340.0 8 1340.0 9 1340.0 10 2271.5 11 3203.0 12 3203.0 13 3203.0 14 6414.5 15 9626.0 16 9626.0 17 9626.0 18 18350.0 19 27074.0 20 27074.0 21 27074.0 22 43905.5 23 60737.0 24 60737.0 25 60737.0 26 87774.0 27 114811.0 28 114811.0 29 114811.0 30 150597.5 31 186384.0 32 186384.0 33 186384.0 34 218302.5 35 250221.0 36 250221.0 37 250221.0 38 276586.5 39 302952.0 40 302952.0 41 302952.0 42 322126.0 43 341300.0 44 341300.0 45 341300.0 46 355675.5 47 370051.0 48 370051.0 49 370051.0 50 355661.0 51 341271.0 52 341271.0 53 341271.0 54 306372.5 55 271474.0 56 271474.0 57 271474.0 58 234503.0 59 197532.0 60 197532.0 61 197532.0 62 160891.0 63 124250.0 64 124250.0 65 124250.0 66 96576.5 67 68903.0 68 68903.0 69 68903.0 70 50555.0 71 32207.0 72 32207.0 73 32207.0 74 22592.5 75 12978.0 76 12978.0 77 12978.0 78 9093.5 79 5209.0 80 5209.0 81 5209.0 82 3545.0 83 1881.0 84 1881.0 85 1881.0 86 1249.5 87 618.0 88 618.0 89 618.0 90 389.5 91 161.0 92 161.0 93 161.0 94 93.5 95 26.0 96 26.0 97 26.0 98 18.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17740380504371422 2 0.24948098193776774 3 0.2541050778077529 4 0.4455279671557076 5 0.25116914392204803 6 0.01677152232208883 7 0.01691831901637407 8 0.015450352073521656 9 0.017211912404944553 10 0.019340464472080553 11 0.018569781827083034 12 0.025542624805632002 13 0.024845340507777106 14 0.022643390093498486 15 0.03835063638201931 16 0.018753277694939587 17 0.038644229770589796 18 0.028772152079907316 19 0.023744365300637796 20 0.026863795054199176 21 0.0315612892713269 22 0.04091957853201104 23 0.026350006624200832 24 0.04554367440199614 25 0.03361644299132028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2724857.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.4280364760705 #Duplication Level Percentage of deduplicated Percentage of total 1 81.36509082167255 57.30383584267543 2 12.361647526273185 17.41213125769389 3 3.298066824013896 6.96829111746506 4 1.2448239989345582 3.5068204001300396 5 0.5739364796817992 2.021060966298862 6 0.3178325183945119 1.343059211926202 7 0.190004817974177 0.9367166375640131 8 0.13699651507274638 0.7718716448510339 9 0.09262136043094184 0.587082649580428 >10 0.3840607587637993 4.624509608605716 >50 0.02117497308266937 1.0043240530996635 >100 0.011267524664699963 1.510424609636548 >500 0.0016857062403977197 0.8427334605920109 >1k 7.901748000476206E-4 1.1671385398812368 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3561 0.13068575708743616 No Hit GTACATGGGAAGCAGTGGTATCAAC 3546 0.1301352694838665 No Hit CCCATGTACTCTGCGTTGATACCAC 2966 0.1088497488125065 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2909 0.1067578959189418 No Hit CTGTAGGACGTGGAATATGGCAAGA 2814 0.10327147442966732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.669917357131035E-5 0.0 4 0.0 0.0 0.0 3.669917357131035E-5 0.0 5 0.0 0.0 0.0 7.33983471426207E-5 0.0 6 0.0 0.0 0.0 7.33983471426207E-5 0.0 7 0.0 0.0 0.0 7.33983471426207E-5 0.0 8 0.0 0.0 0.0 7.33983471426207E-5 0.0 9 0.0 0.0 0.0 7.33983471426207E-5 0.0 10 0.0 0.0 0.0 7.33983471426207E-5 0.0 11 0.0 0.0 0.0 7.33983471426207E-5 0.0 12 0.0 0.0 0.0 7.33983471426207E-5 7.33983471426207E-5 13 0.0 0.0 0.0 7.33983471426207E-5 7.33983471426207E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 755 0.0 12.367096 4 GTCCTAC 2050 0.0 11.180564 1 GGCGAGG 770 0.0 11.092126 19 AATCCCG 120 1.2953751E-7 11.071586 19 GTAGGAC 1810 0.0 11.054048 3 AGGACGT 1545 0.0 11.018473 5 GGACGTG 1550 0.0 10.834501 6 TAGGACT 540 0.0 10.586347 4 TTAGGAC 1125 0.0 10.58615 3 TGTAGGA 2015 0.0 10.503614 2 CTAGACT 320 0.0 10.420935 4 TCCTACA 2290 0.0 10.407953 2 GTCCTAA 1045 0.0 10.235477 1 TAGGACG 1685 0.0 10.17797 4 GTCTTAC 235 0.0 10.159644 1 CTGTAGG 2000 0.0 10.12307 1 GTCCTAT 340 0.0 10.111835 1 GACGTGG 785 0.0 10.032282 7 ATGGCAA 1525 0.0 10.018877 17 TGGCAAG 1530 0.0 9.861903 18 >>END_MODULE