FastQCFastQC Report
Thu 26 May 2016
SRR1513196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724857
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC39100.14349376866382346No Hit
CCCATGTACTCTGCGTTGATACCAC38120.13989724965383504No Hit
GTCCTACAGTGGACATTTCTAAATT35760.1312362446910058No Hit
GTATCAACGCAGAGTACTTTTTTTT33990.12474049096888387No Hit
CTGTAGGACGTGGAATATGGCAAGA30850.11321695046749242No Hit
GTCCTAAAGTGTGTATTTCTCATTT29850.10954703311036137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1251.0004442E-1012.9466796
TGGACCG1352.7284841E-1112.6937575
TAGGACC8700.011.9277554
CGTACAC907.2907333E-611.6359443
AACCGTG907.545759E-611.6000627
AGGACGT17100.011.1348755
GGACGTG16950.010.9517726
GACGTGA9800.010.7499647
GTCCTAA8900.010.7019131
TAGGACG18050.010.5488294
GGCGAGG7900.010.4488119
GTAGGAC20650.010.4193313
TGTAGGA22350.010.3946952
ACCGTGC1201.5468195E-610.2801758
GTCCTAC17350.010.2658181
CAATACT2800.010.2003414
TGGCGAG18900.010.14359218
ACGTGAA10900.010.0988038
GTACTAG1803.765308E-1010.0538521
TTAGGAC12600.09.89810853
Produced by FastQC (version 0.11.5)