FastQCFastQC Report
Thu 26 May 2016
SRR1513195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950413
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC29920.1534034073808983No Hit
CCCATGTACTCTGCGTTGATACCAC25140.1288957774584152No Hit
GTATCAACGCAGAGTACTTTTTTTT20940.10736187668970623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTA658.106446E-411.67824613
TAGGACC3500.011.4346764
TGTGCGA600.005927170611.07057210
AGAGCGT803.8173876E-410.6735526
GTATTAA3150.010.6122621
GCTACAC3150.010.5879353
ATTACAC2100.010.4366793
TTAGGAC3750.010.4185293
GTCCTAA3700.010.0673071
AGGACCG951.6260346E-410.011085
GTCCTAC7650.09.988011
GTAGGAC7300.09.9207513
TAAGACC2500.09.9100524
GCGCCAC1356.5503264E-79.84000413
TGCGCCA1451.6452213E-79.81601912
TAGGACA4600.09.7360464
GTGCTAG2650.09.7312431
TTATACT3550.09.6631074
GTATTAG2500.09.5510361
GTAAGAC2106.366463E-119.5291423
Produced by FastQC (version 0.11.5)