##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513195_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1950413 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.354925854165245 31.0 30.0 34.0 25.0 34.0 2 29.22604340721683 31.0 30.0 34.0 22.0 34.0 3 29.405591533690558 31.0 30.0 34.0 23.0 34.0 4 32.46606180332063 37.0 33.0 37.0 22.0 37.0 5 32.61121772670711 37.0 33.0 37.0 23.0 37.0 6 32.980343650293555 37.0 35.0 37.0 26.0 37.0 7 32.93594023419655 37.0 35.0 37.0 26.0 37.0 8 32.81882914028977 37.0 35.0 37.0 25.0 37.0 9 34.05432131553676 39.0 35.0 39.0 23.0 39.0 10 33.644566048319 39.0 34.0 39.0 18.0 39.0 11 34.08172935680802 39.0 35.0 39.0 25.0 39.0 12 34.07084345725752 39.0 35.0 39.0 25.0 39.0 13 34.107626436042004 39.0 35.0 39.0 25.0 39.0 14 34.34168609417595 39.0 34.0 41.0 17.0 41.0 15 34.76074862093311 40.0 34.0 41.0 19.0 41.0 16 34.604924700563416 39.0 34.0 41.0 17.0 41.0 17 34.213912643117126 39.0 33.0 41.0 17.0 41.0 18 33.93844944634803 39.0 33.0 41.0 13.0 41.0 19 34.57421376908378 39.0 34.0 41.0 18.0 41.0 20 34.78674619170401 40.0 34.0 41.0 19.0 41.0 21 34.832368836754064 40.0 35.0 41.0 19.0 41.0 22 34.51625886414826 40.0 34.0 41.0 17.0 41.0 23 34.06199917658465 39.0 34.0 41.0 10.0 41.0 24 34.30388538222417 39.0 34.0 41.0 10.0 41.0 25 33.94920921876546 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9148957561953956 1101 2 1.2014718427414053 1101 3 1.0978519873941295 1101 4 1.3900630463746566 1101 5 1.2960631653459984 1101 6 1.0203339554382467 1101 7 0.9990859133723617 1101 8 1.0873126908828254 1101 9 1.4171754131177607 1101 10 1.7252278544679598 1101 11 1.308684305389896 1101 12 1.1761788570538485 1101 13 1.1300912858991126 1101 14 1.9843957567141004 1101 15 1.64385598718399 1101 16 1.8068188367459967 1101 17 2.2163123311140964 1101 18 2.3525630214674393 1101 19 1.8567562012953402 1101 20 1.4987734021438328 1101 21 1.5607111242345795 1101 22 1.7363986895757577 1101 23 1.9434016797910374 1101 24 1.5561896635758714 1101 25 1.8239305810475415 1102 1 1.1484304025911456 1102 2 1.4094412881066383 1102 3 1.2605183644282185 1102 4 1.5706249680131066 1102 5 1.6604123348770514 1102 6 1.3345576261971317 1102 7 1.3280695517350125 1102 8 1.302947467408984 1102 9 1.6757702858282073 1102 10 1.9221991115185872 1102 11 1.4641445502551775 1102 12 1.301067406382714 1102 13 1.3405125559195312 1102 14 2.369410442791761 1102 15 1.851818223773833 1102 16 2.0091961947514463 1102 17 2.347357655208029 1102 18 2.5568026464477214 1102 19 2.0469477252412744 1102 20 1.778448295985605 1102 21 1.7351324908525925 1102 22 2.0404236252104084 1102 23 2.2519467578855057 1102 24 1.9501105513509813 1102 25 2.279064544724207 1103 1 0.8808390357419214 1103 2 1.0901534014295606 1103 3 0.8621244064353668 1103 4 1.4029065260386915 1103 5 1.1708208072152075 1103 6 0.764932453409422 1103 7 0.8519859287001523 1103 8 0.8484905362371364 1103 9 1.184238822744824 1103 10 1.4429798792768693 1103 11 0.9559025483441346 1103 12 1.0379333200405512 1103 13 1.036338331671665 1103 14 2.0199216386301444 1103 15 1.4476101436147886 1103 16 1.5569918244139629 1103 17 1.8823452348608924 1103 18 2.0636546494588686 1103 19 1.6275377607088615 1103 20 1.275011133515953 1103 21 1.237836286207994 1103 22 1.4751101963265896 1103 23 1.900099171300404 1103 24 1.4982945944051806 1103 25 1.7244006294769534 1104 1 0.8771761289048108 1104 2 1.1813005636855607 1104 3 0.9888919546036377 1104 4 1.4027101464180518 1104 5 1.2859278036982715 1104 6 1.0120259706947436 1104 7 0.9541138580343684 1104 8 0.9375349511317239 1104 9 1.2717675238193706 1104 10 1.4139110169324312 1104 11 1.0769597654589447 1104 12 1.090839323209991 1104 13 1.013696194172482 1104 14 1.9380376527858516 1104 15 1.58060873878118 1104 16 1.5407757099865123 1104 17 2.0869676532262176 1104 18 2.224044751317784 1104 19 1.5066963389484798 1104 20 1.2678750326140218 1104 21 1.246057445919135 1104 22 1.5005751008087813 1104 23 1.9580977109097333 1104 24 1.7165248689845924 1104 25 1.9531308393177582 1105 1 0.8386967464168116 1105 2 1.073647445145724 1105 3 0.9423170500041458 1105 4 1.3076709728557177 1105 5 1.194698049162767 1105 6 0.8395109295523753 1105 7 0.8399477945575669 1105 8 0.9298101446832803 1105 9 1.1328093726493762 1105 10 1.461541899481233 1105 11 0.996846037629787 1105 12 1.0058892673304811 1105 13 0.9118788651550886 1105 14 1.773166928436865 1105 15 1.4661592822311178 1105 16 1.7356092006317638 1105 17 1.998557931928886 1105 18 1.5197220514978724 1105 19 1.5155952520486053 1105 20 1.3064386107555777 1105 21 1.2485050263074484 1105 22 1.4557055345843253 1105 23 1.7881833542189582 1105 24 1.5778448993274594 1105 25 1.7790507086813108 1106 1 0.6414097517964983 1106 2 0.7702491714377366 1106 3 0.6432548156369009 1106 4 1.1021806112243553 1106 5 1.0108246743360496 1106 6 0.7701010977888245 1106 7 0.7027893875839339 1106 8 0.7541143508799095 1106 9 0.847978607455893 1106 10 0.738153894529411 1106 11 0.7864154915031065 1106 12 0.7338153779249836 1106 13 0.7163744125083156 1106 14 1.6465500842538248 1106 15 1.1701695738067457 1106 16 1.351268255107513 1106 17 1.5521809202510255 1106 18 1.63165035276036 1106 19 1.244101090977459 1106 20 0.975652423373603 1106 21 0.7350407473167593 1106 22 0.9928252625763818 1106 23 1.4881215134873642 1106 24 1.1587905376732763 1106 25 1.4431634596240457 1107 1 0.6820182105957038 1107 2 0.8569062401891685 1107 3 0.7802546657283997 1107 4 1.1924281299558501 1107 5 1.1046909276700987 1107 6 0.8369272520135596 1107 7 0.7257190522131651 1107 8 0.7771281905456959 1107 9 1.1404635529655707 1107 10 1.1788585210451146 1107 11 0.7609480174047505 1107 12 0.7527914180212676 1107 13 0.7491242717923186 1107 14 1.6051082473373057 1107 15 1.0673015696545605 1107 16 1.1875191849644793 1107 17 1.7278472069609307 1107 18 1.9131190034990126 1107 19 1.2875963914874333 1107 20 1.1240913667732428 1107 21 0.9741623400804187 1107 22 1.200092374065882 1107 23 1.4936640549738343 1107 24 1.2437870728070166 1107 25 1.47681740858161 1108 1 0.5704799093247814 1108 2 0.8186341379598439 1108 3 0.6079677013558751 1108 4 1.0664999404982467 1108 5 0.8808753849240745 1108 6 0.5991607404502588 1108 7 0.5865621663183163 1108 8 0.534211321318665 1108 9 0.8691113757310376 1108 10 1.0202363945085509 1108 11 0.6770477465526099 1108 12 0.43898073672395554 1108 13 0.40556985829138625 1108 14 1.4303822246968423 1108 15 0.8847361137201233 1108 16 0.9188477494481262 1108 17 1.2993535615688856 1108 18 1.6010667776499972 1108 19 0.9265407626243132 1108 20 0.7965380644605844 1108 21 0.6896592495880256 1108 22 1.067822077517505 1108 23 1.2392297192560733 1108 24 0.9684346177754222 1108 25 1.2602301455772391 1109 1 0.3102071413404133 1109 2 0.5378644165631634 1109 3 0.4403415970526545 1109 4 0.8317365429762305 1109 5 0.7642446802055929 1109 6 0.4249268656521181 1109 7 0.2530328742937229 1109 8 0.32755415403453725 1109 9 0.4797159435769842 1109 10 0.8983446603284122 1109 11 0.4193658673033269 1109 12 0.4071795180888813 1109 13 0.2914057361021847 1109 14 1.1511953718422774 1109 15 0.740416608773657 1109 16 0.884982722103473 1109 17 1.2215514472777471 1109 18 1.2728099143461122 1109 19 0.6445518633313441 1109 20 0.6594646885157331 1109 21 0.5500340680819633 1109 22 0.8348494493097718 1109 23 1.2495493056258198 1109 24 0.8676564826399158 1109 25 1.1558824138940835 1110 1 0.5844303008421754 1110 2 0.9331971741596021 1110 3 0.789027267349482 1110 4 1.0387759507650642 1110 5 1.0057284641554745 1110 6 0.7294400391376001 1110 7 0.71158628682862 1110 8 0.7070003468934587 1110 9 0.9246942564091256 1110 10 1.3899283357076797 1110 11 0.930011177796942 1110 12 0.8334566243533104 1110 13 0.6074046450052961 1110 14 1.4129888677404239 1110 15 1.148631209849448 1110 16 1.3457272771310897 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94161.0 3 1.0 4 772.0 5 4714.0 6 5597.0 7 4981.0 8 4772.0 9 4392.0 10 4105.0 11 3851.0 12 3811.0 13 3793.0 14 3803.0 15 3946.0 16 3899.0 17 4121.0 18 4759.0 19 5733.0 20 7368.0 21 9214.0 22 11346.0 23 13754.0 24 15869.0 25 18323.0 26 20351.0 27 23086.0 28 25441.0 29 30576.0 30 36698.0 31 43828.0 32 56455.0 33 73238.0 34 95188.0 35 127859.0 36 183253.0 37 264976.0 38 732379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.00760316452706 18.140254863169744 12.137021309815088 25.71512066248811 2 14.770017359799187 20.0163573825926 40.61750918496334 24.596116072644875 3 18.520709967917067 24.496175597127596 29.646357756836967 27.33675667811837 4 11.490785768407216 16.444687568088785 38.76817696928864 33.29634969421535 5 12.920174384803168 37.71937535244246 36.08800048114661 13.27244978160776 6 32.33581328754956 36.7583983064928 18.013154628940185 12.892633777017451 7 28.24245059799325 31.432568453073863 22.243543918991683 18.0814370299412 8 25.639149294916603 35.717952281296974 20.742256748919132 17.9006416748673 9 26.55643280431746 14.819264875109997 19.767891464567832 38.85641085600471 10 13.800459796522347 28.554358913083277 35.53402499393587 22.1111562964585 11 34.818651096052406 22.319920328123413 24.006024621563714 18.855403954260467 12 22.985768136010464 24.642408390389004 31.47047208759648 20.901351386004052 13 28.9066073945215 21.32161224611206 25.613782620023017 24.157997739343426 14 20.78132876970156 20.942002934495918 28.57697461778986 29.699693678012668 15 23.81150706740786 29.129937927447642 23.33747423901882 23.72108076612568 16 22.902161287593046 27.606634888807406 26.799145638834972 22.692058184764576 17 22.135076856160392 27.635390764129962 27.673449540832657 22.55608283887699 18 22.010974509575643 27.214352717804115 28.68707466595138 22.087598106668867 19 23.56774886724089 26.869276906073946 27.333611982245102 22.229362244440058 20 23.79691705507779 26.74379841462614 27.2652300925622 22.194054437733868 21 23.60738338507557 26.484501179394677 26.910509101521292 22.99760633400847 22 23.462630618948648 26.819432447460045 26.847490002892954 22.87044693069835 23 22.79344639629752 26.758616657469275 27.290766159886925 23.15717078634628 24 23.188785677609776 26.809188169111476 27.194670242271506 22.807355911007242 25 23.128682625393907 26.52685341714811 27.148591370141805 23.195872587316178 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 167.0 1 167.0 2 273.5 3 380.0 4 380.0 5 380.0 6 865.0 7 1350.0 8 1350.0 9 1350.0 10 2182.5 11 3015.0 12 3015.0 13 3015.0 14 5435.0 15 7855.0 16 7855.0 17 7855.0 18 14530.5 19 21206.0 20 21206.0 21 21206.0 22 33193.5 23 45181.0 24 45181.0 25 45181.0 26 63632.5 27 82084.0 28 82084.0 29 82084.0 30 104202.5 31 126321.0 32 126321.0 33 126321.0 34 147998.0 35 169675.0 36 169675.0 37 169675.0 38 189575.0 39 209475.0 40 209475.0 41 209475.0 42 224108.0 43 238741.0 44 238741.0 45 238741.0 46 250874.0 47 263007.0 48 263007.0 49 263007.0 50 256751.0 51 250495.0 52 250495.0 53 250495.0 54 226204.5 55 201914.0 56 201914.0 57 201914.0 58 174474.0 59 147034.0 60 147034.0 61 147034.0 62 120032.0 63 93030.0 64 93030.0 65 93030.0 66 71933.5 67 50837.0 68 50837.0 69 50837.0 70 37134.5 71 23432.0 72 23432.0 73 23432.0 74 16463.5 75 9495.0 76 9495.0 77 9495.0 78 6641.0 79 3787.0 80 3787.0 81 3787.0 82 2590.0 83 1393.0 84 1393.0 85 1393.0 86 901.5 87 410.0 88 410.0 89 410.0 90 257.5 91 105.0 92 105.0 93 105.0 94 61.0 95 17.0 96 17.0 97 17.0 98 12.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17068179918817195 2 0.2622008774551851 3 0.2636364708397657 4 0.46215852745034003 5 0.2594322330706368 6 0.019329239499531636 7 0.020047036191821938 8 0.01845762923032199 9 0.019329239499531636 10 0.021533900768708987 11 0.021277544807176738 12 0.028865681268531333 13 0.027532630268563632 14 0.025840680922450787 15 0.03891483496059553 16 0.021431358384096086 17 0.04086314026824062 18 0.03189068161461188 19 0.02707118953780558 20 0.03019873226849903 21 0.035069495537611774 22 0.043529242268176026 23 0.029993647499273232 24 0.04645170022964367 25 0.036761444883724625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1950413.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.266899171572 #Duplication Level Percentage of deduplicated Percentage of total 1 81.11569302790129 57.80863916251627 2 12.49683178194363 17.812209011357464 3 3.4491987601528726 7.374411007875778 4 1.2733092066851999 3.629791953882739 5 0.5594844671398638 1.9936361553858681 6 0.31912241924658274 1.3645719165500603 7 0.19092512078128543 0.9524648932428054 8 0.118406754573399 0.6750785791532399 9 0.08344114338716241 0.5351932397280227 >10 0.3638921681644717 4.501277080241891 >50 0.01904627622554452 0.9384886375868748 >100 0.008899457166527482 1.168937922631384 >500 0.0012391701144473495 0.6096112406812971 >1k 5.102465177136085E-4 0.635689199166337 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2992 0.1534034073808983 No Hit CCCATGTACTCTGCGTTGATACCAC 2514 0.1288957774584152 No Hit GTATCAACGCAGAGTACTTTTTTTT 2094 0.10736187668970623 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.0254238461289994E-4 0.0 12 0.0 0.0 0.0 1.538135769193499E-4 0.0 13 5.127119230644997E-5 0.0 0.0 1.538135769193499E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTTA 65 8.106446E-4 11.678246 13 TAGGACC 350 0.0 11.434676 4 TGTGCGA 60 0.0059271706 11.070572 10 AGAGCGT 80 3.8173876E-4 10.673552 6 GTATTAA 315 0.0 10.612262 1 GCTACAC 315 0.0 10.587935 3 ATTACAC 210 0.0 10.436679 3 TTAGGAC 375 0.0 10.418529 3 GTCCTAA 370 0.0 10.067307 1 AGGACCG 95 1.6260346E-4 10.01108 5 GTCCTAC 765 0.0 9.98801 1 GTAGGAC 730 0.0 9.920751 3 TAAGACC 250 0.0 9.910052 4 GCGCCAC 135 6.5503264E-7 9.840004 13 TGCGCCA 145 1.6452213E-7 9.816019 12 TAGGACA 460 0.0 9.736046 4 GTGCTAG 265 0.0 9.731243 1 TTATACT 355 0.0 9.663107 4 GTATTAG 250 0.0 9.551036 1 GTAAGAC 210 6.366463E-11 9.529142 3 >>END_MODULE