FastQCFastQC Report
Thu 26 May 2016
SRR1513195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950413
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC34220.17545002007267177No Hit
CCCATGTACTCTGCGTTGATACCAC31770.16288857795759154No Hit
GTATCAACGCAGAGTACTTTTTTTT29900.1533008649962854No Hit
TATCAACGCAGAGTACTTTTTTTTT21420.10982289392041583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA500.001477844113.32603556
AAAGGCG1950.011.7167025
AGGACGT5500.011.5968725
ACCGTTC600.005927163611.0705748
TTAGGAC4100.010.9143693
TAGGACG6150.010.8355674
GGACGTG5750.010.7601526
TTTATAC2350.010.5339223
GACGTGA3250.010.512587
AAATCCG1002.4333593E-510.43663319
AAGGCGT1658.9858077E-1010.3839246
AGGACCG1303.7336758E-710.2521145
GGCGTGC1502.4891051E-810.1216688
GGACCGC856.477029E-410.0785146
TAGGACT2850.010.0208644
ACGTGAA3700.010.00198658
GCGGCTT951.6613802E-49.98976911
GTCTTAA2201.8189894E-129.9574061
GTAGGAC7800.09.8872393
CCTACAC2800.09.8610823
Produced by FastQC (version 0.11.5)