FastQCFastQC Report
Thu 26 May 2016
SRR1513194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019023
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3300.011.8276654
GTATAAG2650.011.1613631
GTAGGAC7900.010.9661513
GGCGTGC803.8055624E-410.6771758
AGGACGT8150.010.6181565
GTCTTAA2700.010.6012951
GGACGTG7750.010.5312296
TTAGGAC6600.010.5297693
TAGGACG8350.010.4889134
GTGCGCC1002.4224244E-510.44093911
TGTAGGA8600.010.4126392
GTCCTAC8550.010.378111
GTCTTAG2700.010.2479191
TGGACCG1303.789064E-710.2411815
CCTACAC2650.010.0589363
AGGACCT7600.09.8850495
GTATTAA2550.09.7282471
ATTATAC2450.09.7143523
CGTGAAA4800.09.68950759
TGGCGAG8350.09.66299218
Produced by FastQC (version 0.11.5)