##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513194_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2019023
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.276653113907074	31.0	30.0	34.0	23.0	34.0
2	29.205967935976954	31.0	30.0	34.0	22.0	34.0
3	29.364595153200334	31.0	30.0	34.0	22.0	34.0
4	32.4672066638171	37.0	33.0	37.0	20.0	37.0
5	32.574104901231934	37.0	33.0	37.0	23.0	37.0
6	32.92335698998971	37.0	35.0	37.0	26.0	37.0
7	32.87601280421273	37.0	35.0	37.0	26.0	37.0
8	32.80269318378245	37.0	35.0	37.0	25.0	37.0
9	34.015992388397756	39.0	35.0	39.0	23.0	39.0
10	33.63877578412925	39.0	34.0	39.0	17.0	39.0
11	34.030528626964625	39.0	35.0	39.0	25.0	39.0
12	34.019239008173756	39.0	35.0	39.0	25.0	39.0
13	34.04818865362108	39.0	35.0	39.0	25.0	39.0
14	34.33301304640908	40.0	34.0	41.0	16.0	41.0
15	34.726354281253855	40.0	34.0	41.0	18.0	41.0
16	34.54546926904745	39.0	34.0	41.0	17.0	41.0
17	34.19143764087878	39.0	33.0	41.0	16.0	41.0
18	33.93778030265133	39.0	33.0	41.0	11.0	41.0
19	34.546823884621425	39.0	34.0	41.0	18.0	41.0
20	34.74499745669068	40.0	35.0	41.0	18.0	41.0
21	34.78634616841908	40.0	35.0	41.0	18.0	41.0
22	34.49164769296833	40.0	35.0	41.0	16.0	41.0
23	34.024225083121884	39.0	34.0	41.0	10.0	41.0
24	34.257892059674404	40.0	34.0	41.0	2.0	41.0
25	33.88350553708403	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7734836141803463
1101	2	1.0049143494326955
1101	3	0.9984047718539024
1101	4	1.3847408118778333
1101	5	1.1836829459638096
1101	6	0.9120379530368155
1101	7	0.8617112607888018
1101	8	0.893670251904652
1101	9	1.102313195142301
1101	10	1.4713930679165799
1101	11	1.1198852521453873
1101	12	1.0006745970382127
1101	13	1.0056733577572388
1101	14	1.98680272885575
1101	15	1.5499887475026313
1101	16	1.608624522822339
1101	17	2.0176988727457044
1101	18	2.0257742995434356
1101	19	1.5313239337903255
1101	20	1.1632868909978953
1101	21	1.2492494592838952
1101	22	1.5267119963832556
1101	23	1.7179142130628335
1101	24	1.2971108411567513
1101	25	1.4838106512441556
1102	1	0.8089114153069481
1102	2	1.000419842972093
1102	3	0.8664719114342141
1102	4	1.2898633199566802
1102	5	1.2038049916331275
1102	6	0.8056409453402651
1102	7	0.7976683019919619
1102	8	0.8050847645611654
1102	9	1.1386648638698347
1102	10	1.3157939735542072
1102	11	0.9505048314402487
1102	12	0.9187675972996701
1102	13	0.9450335808707919
1102	14	1.8186247632217274
1102	15	1.5058666957439755
1102	16	1.5667922545532633
1102	17	1.9740597820521657
1102	18	2.162133732184067
1102	19	1.5806327742613178
1102	20	1.3746677980893551
1102	21	1.1919188600658401
1102	22	1.384726771414357
1102	23	1.735155428328362
1102	24	1.4118177096699327
1102	25	1.729486170674683
1103	1	0.5411095224413067
1103	2	0.8240820459251594
1103	3	0.5043318023129153
1103	4	0.998471595555408
1103	5	0.9006107855311356
1103	6	0.5881194403435757
1103	7	0.5086149178902843
1103	8	0.5888412483788485
1103	9	0.9311884891958684
1103	10	1.087375283420819
1103	11	0.6098979277973555
1103	12	0.6284104441678053
1103	13	0.5787353844771062
1103	14	1.4159938361539517
1103	15	1.1135508440123445
1103	16	1.183674709098817
1103	17	1.4384709413735237
1103	18	1.7043869454637885
1103	19	0.9628498802938523
1103	20	0.6941481884989642
1103	21	0.7653924751358332
1103	22	1.0441605930399191
1103	23	1.3252759639142084
1103	24	1.060139697840178
1103	25	1.2505962443500565
1104	1	0.5604636311859288
1104	2	0.7878865771544739
1104	3	0.6060750739660179
1104	4	1.0671654751376494
1104	5	0.866353798031497
1104	6	0.5664482619779463
1104	7	0.47032538230889287
1104	8	0.541326532540765
1104	9	0.8649042606118869
1104	10	0.951239156871658
1104	11	0.6220120977245642
1104	12	0.6741633908565206
1104	13	0.6566859933451994
1104	14	1.6319435605224797
1104	15	1.1860033772773235
1104	16	1.2269411040306935
1104	17	1.5810034457936766
1104	18	1.8364869558862367
1104	19	1.1030762070197326
1104	20	0.8835262125344414
1104	21	0.8363042702474246
1104	22	1.1989725242067024
1104	23	1.5043667509404273
1104	24	1.3737209915193844
1104	25	1.5750822346092903
1105	1	0.4507595386997387
1105	2	0.7395099861582963
1105	3	0.5746934453222892
1105	4	1.008978713313823
1105	5	1.008462032297139
1105	6	0.6281693460861888
1105	7	0.6074889247624213
1105	8	0.7167210337411234
1105	9	0.8525691103151445
1105	10	1.1631633549971738
1105	11	0.6377574066329927
1105	12	0.616079796542472
1105	13	0.6098414541847319
1105	14	1.3427397397934442
1105	15	0.940647752381409
1105	16	1.234583039285205
1105	17	1.5200412775390149
1105	18	1.0034270657629776
1105	19	0.9437729742309742
1105	20	0.7269186007573083
1105	21	0.7379449772264053
1105	22	0.8847633232109118
1105	23	1.1948860767154557
1105	24	1.0340357144583123
1105	25	1.2700041694438795
1106	1	0.40686639926384416
1106	2	0.729478060313479
1106	3	0.6420723526693024
1106	4	1.2090287076330881
1106	5	1.1324651439466322
1106	6	0.7070913798879701
1106	7	0.6564485756583593
1106	8	0.716299445559585
1106	9	0.7421928906405668
1106	10	0.660302967095916
1106	11	0.745572850768788
1106	12	0.7289650010046174
1106	13	0.5970392009239447
1106	14	1.5910735252606756
1106	15	1.1855210174509665
1106	16	1.3228741346490835
1106	17	1.6210492000906243
1106	18	1.6087061486347736
1106	19	1.0948696242771447
1106	20	0.981436889915777
1106	21	0.8419659366185357
1106	22	1.1084463355291803
1106	23	1.5197699369113877
1106	24	1.0177472365340279
1106	25	1.2963798994952
1107	1	0.7485921287783484
1107	2	0.8856502325964222
1107	3	0.8338362717831949
1107	4	1.1708192536065027
1107	5	0.9973484875883756
1107	6	0.704750508472543
1107	7	0.7312540825261351
1107	8	0.8419543781342611
1107	9	1.0908921153404165
1107	10	1.3151780480082778
1107	11	0.834908283989023
1107	12	0.8082889909892259
1107	13	0.726274179077727
1107	14	1.5538429708335073
1107	15	1.0349781530249373
1107	16	1.2449920055278696
1107	17	1.5847544484622915
1107	18	1.848539279328456
1107	19	1.2197517314735649
1107	20	1.0571180710767507
1107	21	0.7939223196452119
1107	22	1.1016442865680105
1107	23	1.4411356839318543
1107	24	1.2512882343416436
1107	25	1.4803372754420465
1108	1	0.5896792594770552
1108	2	0.7531945101049331
1108	3	0.6298983088832415
1108	4	1.048560317241268
1108	5	0.9688736031707208
1108	6	0.6839769126344848
1108	7	0.5784554762633363
1108	8	0.6105093727559634
1108	9	0.8042649968909572
1108	10	1.1258896162678766
1108	11	0.648479130663091
1108	12	0.5096803477910399
1108	13	0.5848261519938376
1108	14	1.4711014465216579
1108	15	0.9687087048208625
1108	16	1.0784702631099279
1108	17	1.4517302308501954
1108	18	1.663486276259981
1108	19	1.090461098564866
1108	20	0.7902275349522512
1108	21	0.8185748445056689
1108	22	1.1156161043325596
1108	23	1.350534644565947
1108	24	1.0408192986793239
1108	25	1.3245491689760698
1109	1	0.44699198042614086
1109	2	0.5822518164349653
1109	3	0.5455071108556808
1109	4	0.8208940441204504
1109	5	0.8503683710291199
1109	6	0.5453436603648711
1109	7	0.3801251870239568
1109	8	0.4383271778464959
1109	9	0.6849541730346758
1109	10	0.9887582291364936
1109	11	0.5679620046914167
1109	12	0.4665189637205671
1109	13	0.4143762315439119
1109	14	1.463006847517974
1109	15	0.9327572825058041
1109	16	1.0008660491420827
1109	17	1.3344804772368093
1109	18	1.5695734550744334
1109	19	0.9282840500241818
1109	20	0.788884601791132
1109	21	0.6933835374961816
1109	22	0.8826764850293074
1109	23	1.2330317770630757
1109	24	0.9861324030833387
1109	25	1.2553708725250488
1110	1	0.4657072158080453
1110	2	0.7405478413976603
1110	3	0.5769343256162287
1110	4	0.8558146790410888
1110	5	0.8397592270866454
1110	6	0.4693125926671371
1110	7	0.47913142769686345
1110	8	0.5750793145986322
1110	9	0.6655572119710484
1110	10	1.045558527603454
1110	11	0.6849983633944419
1110	12	0.519615558483828
1110	13	0.38951189834624955
1110	14	1.2542970388081613
1110	15	0.9123292537444385
1110	16	1.0317709467136424
1110	17	1.3894865822429097
1110	18	0.9063798585475027
1110	19	0.7259166643234636
1110	20	0.626091222331091
1110	21	0.49708724119988545
1110	22	0.7950391223919411
1110	23	-0.39458320055189944
1110	24	0.406702881987151
1110	25	1.0022912361120788
1111	1	0.49290226837169016
1111	2	0.8751411366209148
1111	3	0.7109317213003976
1111	4	1.2253269998989111
1111	5	1.1111421111564042
1111	6	0.8585834415137157
1111	7	0.7263339838977672
1111	8	0.8058677120279185
1111	9	1.0414824696341185
1111	10	1.2782504240570773
1111	11	0.8358633819339616
1111	12	0.7857736771138519
1111	13	0.6424744985379434
1111	14	1.5597569373085776
1111	15	1.165684792497963
1111	16	1.354920335921392
1111	17	1.6797862694958923
1111	18	1.8668881965586763
1111	19	1.3740961253599195
1111	20	0.9147620665952942
1111	21	0.7091927274470393
1111	22	1.1420018676921444
1111	23	1.555330307140757
1111	24	1.1540696399194346
1111	25	1.2995416208405501
1112	1	0.1930486882895046
1112	2	0.30395787054989754
1112	3	0.21352723125298212
1112	4	0.6681231840595743
1112	5	0.3823616023092029
1112	6	0.19277085018508444
1112	7	0.07335820862464715
1112	8	0.21437320436795915
1112	9	0.5105714946107938
1112	10	0.7254723426334877
1112	11	0.1923319119456366
1112	12	0.14382120893089478
1112	13	0.10784503602697981
1112	14	0.8204237043009144
1112	15	0.4767561872812536
1112	16	0.429691159583669
1112	17	0.8972432295399742
1112	18	1.0203709759194979
1112	19	0.5347895096992161
1112	20	0.41233488807818475
1112	21	0.3037726905160838
1112	22	0.6102574706927015
1112	23	0.9158529090918179
1112	24	0.6623117528760005
1112	25	0.8495129692027987
1113	1	0.06677271108553384
1113	2	0.2071272896368015
1113	3	0.19974025567460174
1113	4	0.5555357390131945
1113	5	0.5907204178665211
1113	6	0.1837446596445318
1113	7	0.1968370664973378
1113	8	0.23090129364236844
1113	9	0.33950661733607745
1113	10	0.6588720530160046
1113	11	0.2283474119387492
1113	12	0.058493818247725926
1113	13	-0.017075278359477863
1113	14	0.644540098088882
1113	15	0.25988981988938775
1113	16	0.4502123792415489
1113	17	0.8025019954675301
1113	18	0.5900443834951474
1113	19	0.5130810521851998
1113	20	0.2898131246861837
1113	21	0.21836297460951215
1113	22	0.47604348530007456
1113	23	0.5228088293924316
1113	24	0.48700472926947214
1113	25	0.8877754275237422
1114	1	-0.8459764890129158
1114	2	-0.5887781340067484
1114	3	-0.48751378981227944
1114	4	-0.29574748428578346
1114	5	-3.5821353997099443
1114	6	-1.3466515992774468
1114	7	-0.9041885384662436
1114	8	-0.6344839979058605
1114	9	-0.25329548580321415
1114	10	-0.13235431844664447
1114	11	-0.4959969122086747
1114	12	-0.5644908761127994
1114	13	-0.5694455022778229
1114	14	-6.036239004669056
1114	15	-2.2740049095505057
1114	16	-1.1197976009333388
1114	17	-0.2382409190140038
1114	18	-6.205439114055785
1114	19	-2.33302816717039
1114	20	-1.3640817129398854
1114	21	-0.8700007905940268
1114	22	-0.7559969330558758
1114	23	-0.41724115262161376
1114	24	-0.4593714394793267
1114	25	-0.332433479985184
1115	1	-0.5445453277235437
1115	2	-0.15127063288552733
1115	3	-0.22146918103917912
1115	4	-3.0827303806766118
1115	5	-0.7645336108109717
1115	6	-0.3863806956265847
1115	7	-0.27377704537990866
1115	8	-0.02070425398623854
1115	9	0.10726409257586766
1115	10	-0.1993091685400259
1115	11	-0.24911411499056868
1115	12	-0.2286783651901132
1115	13	-0.3627089163881365
1115	14	-6.241058251335403
1115	15	-2.334728091331364
1115	16	-1.0396047721105006
1115	17	-0.01509177461557698
1115	18	0.29409450044238383
1115	19	0.13633609569837546
1115	20	-0.05918645491184549
1115	21	-0.1308654672088494
1115	22	-1.4492515037390703
1115	23	-0.5294848774731236
1115	24	-0.18225738664195745
1115	25	0.18534307187835708
1116	1	0.38663054620730364
1116	2	0.6921502969915814
1116	3	0.6100600168162664
1116	4	0.7498427107290837
1116	5	0.17300566670115103
1116	6	0.3437562471686846
1116	7	0.40958993312489866
1116	8	0.4072446609321645
1116	9	0.4945644794457138
1116	10	0.6870096527078431
1116	11	0.48101310147194454
1116	12	0.14425444173026847
1116	13	0.29689504322177385
1116	14	1.2813198568730257
1116	15	0.8905161090946123
1116	16	0.8195674574230125
1116	17	1.1758262125496373
1116	18	1.1458860313870076
1116	19	0.7443855292883654
1116	20	0.3775729295105421
1116	21	0.2830646262631973
1116	22	0.36500154367977444
1116	23	-0.19239618721314855
1116	24	0.1335705326969716
1116	25	0.7646120883476186
1201	1	0.7930283195659307
1201	2	1.010409291144164
1201	3	0.9265840798636837
1201	4	1.3798481168676275
1201	5	1.2234602217071782
1201	6	0.9464121321229939
1201	7	0.9326600085242944
1201	8	0.9435049502656483
1201	9	1.1095033786183635
1201	10	1.5172399007216129
1201	11	0.979952695992452
1201	12	1.0954534392067643
1201	13	0.838477609093566
1201	14	1.8738488790496604
1201	15	1.496427903072977
1201	16	1.495322167736127
1201	17	1.9811097100729356
1201	18	1.222375059600175
1201	19	1.2783219431655226
1201	20	1.188547199729598
1201	21	1.1464312560611418
1201	22	1.308945426815491
1201	23	1.6371014621672444
1201	24	1.331236532670232
1201	25	1.6417683830604872
1202	1	0.7184941983678002
1202	2	0.8862114498224756
1202	3	0.8913493898994211
1202	4	1.264295101033376
1202	5	1.092149464757874
1202	6	0.7643485852431979
1202	7	0.7425228826535459
1202	8	0.8291707462615747
1202	9	1.035096709878367
1202	10	1.3242528386724857
1202	11	0.8937043964715414
1202	12	0.9213413611783849
1202	13	0.9404599908899982
1202	14	1.6571148292096396
1202	15	1.2419297662065887
1202	16	1.3328695092648317
1202	17	1.7314660236441455
1202	18	1.8796048384472641
1202	19	1.340865230256
1202	20	1.1994522075808973
1202	21	0.9850924710188735
1202	22	1.2130634004740415
1202	23	1.511153820705509
1202	24	1.2229410566600976
1202	25	1.6168689808277819
1203	1	0.5218345552687147
1203	2	0.700918060101209
1203	3	0.5369458522944228
1203	4	0.9121005103269226
1203	5	0.9283826990434534
1203	6	0.6254111154323283
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2211	11	-0.48166557691910583
2211	12	-0.6401266413258924
2211	13	-0.6392339228125081
2211	14	-0.15716154130713278
2211	15	-0.46908294076298773
2211	16	-7.265818581081998
2211	17	-2.3231966004829125
2211	18	-0.8129930426306586
2211	19	-0.7570588586662765
2211	20	-0.8082559383131596
2211	21	-0.7568482965309613
2211	22	-0.6644889176798046
2211	23	-0.08693888245981896
2211	24	-0.5832531391166995
2211	25	-8.029363227774084
2212	1	-1.4938127492069277
2212	2	-3.7723983018391287
2212	3	-1.712778729855227
2212	4	-3.6294108734869184
2212	5	-1.215168952682589
2212	6	-0.9459504678502384
2212	7	-0.8066247871919572
2212	8	-0.7422836887316748
2212	9	-0.6972333487986191
2212	10	-0.3106740219306374
2212	11	-0.7617831820019987
2212	12	-0.9164736869832524
2212	13	-1.1207674773055132
2212	14	-6.63367349303158
2212	15	-5.178462787161134
2212	16	-2.7501478476483854
2212	17	-7.547494949697747
2212	18	-3.0533268054280107
2212	19	-1.9652967107227894
2212	20	-1.895693687010585
2212	21	-1.487663995049985
2212	22	-1.3973844319525952
2212	23	-0.8446689811553938
2212	24	-1.103176184181649
2212	25	-1.006214499414547
2213	1	-0.2735824287701121
2213	2	-0.29298624537139517
2213	3	-0.19328768627498505
2213	4	0.11959117229734773
2213	5	-0.11764442440989598
2213	6	-0.36362646392317544
2213	7	-0.44384113161836325
2213	8	-0.4524111359979983
2213	9	-0.3257165864191691
2213	10	-4.288126828475672
2213	11	-1.8281960575755605
2213	12	-1.147383975783221
2213	13	-0.8769890387535142
2213	14	-0.15119372728497638
2213	15	-0.7288384401638481
2213	16	-0.39418728004572046
2213	17	0.0892854532161067
2213	18	0.16870318989020205
2213	19	-0.39775308594602166
2213	20	-0.8266804520915372
2213	21	-0.7769203532518816
2213	22	-1.0347812136118435
2213	23	-1.2845526649965677
2213	24	-0.7403754888489473
2213	25	-0.47576053536327834
2214	1	-0.7939360715646835
2214	2	-0.49553123540029276
2214	3	-0.54223025018808
2214	4	-0.18795992216048774
2214	5	-3.4195687045325656
2214	6	-1.2839823378548516
2214	7	-1.0311442975830083
2214	8	-0.6720548612179584
2214	9	-0.44453513125263555
2214	10	-4.095991511144636
2214	11	-1.633237217328194
2214	12	-1.1541053708565343
2214	13	-0.7671418395018108
2214	14	0.05795240330877505
2214	15	-0.14954741990455744
2214	16	-0.1504041639677851
2214	17	0.07131449467989626
2214	18	0.5691302824746387
2214	19	-0.24769550180993605
2214	20	-0.6019562575083981
2214	21	-0.6006569293224047
2214	22	-0.28220174495147177
2214	23	-0.02640650925525989
2214	24	-0.560570134762763
2214	25	-0.339880882142495
2215	1	-2.0336396810565844
2215	2	-4.766206462874376
2215	3	-2.8058844054294525
2215	4	-1.5935083383670374
2215	5	-1.5612926629723383
2215	6	-1.8612692302905742
2215	7	-1.9333485732583178
2215	8	-2.054138394406312
2215	9	-8.212237878412694
2215	10	-3.7050438316868473
2215	11	-2.862204755262578
2215	12	-3.1674292393765704
2215	13	-2.4519601507499154
2215	14	-7.463297344743669
2215	15	-4.309205837116892
2215	16	-9.114982641174695
2215	17	-4.216326871442881
2215	18	-2.5091752194189247
2215	19	-3.1638844198038605
2215	20	-2.858819349104305
2215	21	-2.921273672905709
2215	22	-9.087901990868318
2215	23	-10.348727480228096
2215	24	-6.384553058027652
2215	25	-4.63745970115659
2216	1	-1.2456096566199406
2216	2	-1.3619212944048122
2216	3	-1.2119545632726592
2216	4	-6.442333469107059
2216	5	-2.9710432420491593
2216	6	-1.976057777703236
2216	7	-1.480539354196143
2216	8	-7.095702578405486
2216	9	-2.961809043172984
2216	10	-1.4571163695158873
2216	11	-1.2924707724080235
2216	12	-1.44797703410876
2216	13	-1.251640365028237
2216	14	-0.5200856772412195
2216	15	-1.0589670425518563
2216	16	-0.7209039504056989
2216	17	-0.33829119553486464
2216	18	-6.36697405091752
2216	19	-3.4373549406148065
2216	20	-2.7834766789034795
2216	21	-1.8961226353433247
2216	22	-1.3775946224640236
2216	23	-0.9611469865601308
2216	24	-1.3744699575190182
2216	25	-0.6773383935175517
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102996.0
3	0.0
4	838.0
5	4893.0
6	5904.0
7	5217.0
8	4760.0
9	4432.0
10	4165.0
11	4018.0
12	3917.0
13	3848.0
14	3825.0
15	3799.0
16	3954.0
17	4246.0
18	4895.0
19	5793.0
20	7537.0
21	9435.0
22	11522.0
23	13542.0
24	15858.0
25	18178.0
26	20412.0
27	22469.0
28	25594.0
29	30576.0
30	36922.0
31	44333.0
32	56287.0
33	73215.0
34	97720.0
35	132430.0
36	190692.0
37	280284.0
38	760517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.620009485425584	18.417402382469028	12.323661374275446	25.63892675782994
2	14.74940108983032	20.069461167036977	40.9748395665504	24.2062981765823
3	18.50817674763148	24.593247890151858	29.524865872343636	27.373709489873026
4	11.540292911028251	16.614743367353533	38.82761033866781	33.017353382950404
5	12.8261496628225	37.8461449860765	36.15556584903047	13.172139502070527
6	32.02321943235443	37.06251789558785	18.119301276989518	12.7949613950682
7	28.15274413351365	31.37684713202257	22.439180252344364	18.031228482119417
8	25.36354111711337	36.15204947775408	20.70783703252365	17.7765723726089
9	26.47419283248723	14.982731152881312	19.917133311933043	38.62594270269842
10	13.837266560124917	28.78965468385734	35.52105553612528	21.852023219892462
11	34.61507023379892	22.372468251746866	24.2980459374373	18.714415577016915
12	22.8839636181501	24.689976957897507	31.632828481247515	20.79323094270488
13	28.751180333099164	21.566877945878566	25.80042189786663	23.881519823155635
14	20.753975744010685	21.23441891295398	28.634868641051288	29.376736701984047
15	23.485459209332586	29.669178617384933	23.394289717815557	23.451072455466925
16	22.621002059841416	27.811079152799813	27.044409943119053	22.523508844239725
17	21.92897224045882	27.91388472543943	27.798139454223392	22.359003579878358
18	21.834767297491993	27.354100793989737	28.918546938338714	21.892584970179556
19	23.364405881193083	27.113076925363462	27.413894065995475	22.10862312744798
20	23.760978732174422	27.042361259740154	27.352203276973196	21.84445673111222
21	23.47562032975254	26.65656606693943	26.95126047755556	22.91655312575247
22	23.30033778443734	27.207874931063138	27.007939445561803	22.48384783893772
23	22.643808965156094	27.03646692848356	27.44489732018337	22.87482678617698
24	23.05073659486539	26.938213243346365	27.39929734845669	22.61175281333155
25	22.976595223915503	26.843996896407184	27.285059847218413	22.894348032458904
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	166.0
2	288.0
3	410.0
4	410.0
5	410.0
6	840.0
7	1270.0
8	1270.0
9	1270.0
10	2136.5
11	3003.0
12	3003.0
13	3003.0
14	5612.5
15	8222.0
16	8222.0
17	8222.0
18	15145.5
19	22069.0
20	22069.0
21	22069.0
22	34909.0
23	47749.0
24	47749.0
25	47749.0
26	67925.5
27	88102.0
28	88102.0
29	88102.0
30	112868.5
31	137635.0
32	137635.0
33	137635.0
34	160946.5
35	184258.0
36	184258.0
37	184258.0
38	203770.5
39	223283.0
40	223283.0
41	223283.0
42	236889.0
43	250495.0
44	250495.0
45	250495.0
46	259514.5
47	268534.0
48	268534.0
49	268534.0
50	261484.0
51	254434.0
52	254434.0
53	254434.0
54	228766.0
55	203098.0
56	203098.0
57	203098.0
58	174624.0
59	146150.0
60	146150.0
61	146150.0
62	119556.5
63	92963.0
64	92963.0
65	92963.0
66	71560.0
67	50157.0
68	50157.0
69	50157.0
70	36589.5
71	23022.0
72	23022.0
73	23022.0
74	16000.0
75	8978.0
76	8978.0
77	8978.0
78	6155.0
79	3332.0
80	3332.0
81	3332.0
82	2258.5
83	1185.0
84	1185.0
85	1185.0
86	781.5
87	378.0
88	378.0
89	378.0
90	240.0
91	102.0
92	102.0
93	102.0
94	59.5
95	17.0
96	17.0
97	17.0
98	14.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1633958602749944
2	0.2450690259595854
3	0.24254305176315474
4	0.3507141820573614
5	0.23783780571098
6	0.018276166244762936
7	0.017483703751765087
8	0.016740770164579602
9	0.01778087718663928
10	0.020257322477257565
11	0.0202077935714452
12	0.02694372476192693
13	0.026002675551491983
14	0.02327858573181187
15	0.03982124027314201
16	0.020851669347005955
17	0.03952406683826781
18	0.0301631036397307
19	0.025804559928242522
20	0.02847912084211027
21	0.032738606741973715
22	0.043189205868382874
23	0.027934302878174243
24	0.047201047239184495
25	0.03551222546746619
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2019023.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.31875962900399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.06125788965386	63.31389677102555
2	10.960965806957738	16.511326978319634
3	2.7370843873320183	6.184614031612798
4	0.9636765558966358	2.903316914947406
5	0.43454351342447817	1.6364639217980572
6	0.22524869651927548	1.0179271457929069
7	0.15280207678317972	0.8056204024431449
8	0.08878988569117134	0.5350035246288061
9	0.07304394188287734	0.49514211909282385
>10	0.27843568233871585	3.5742719599212833
>50	0.014053533319892654	0.7160935085336382
>100	0.008504932655061685	1.2157124222787157
>500	9.956859656883748E-4	0.45096290925279847
>1k	5.974115794129312E-4	0.6396473903524966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	4.9528905812365684E-5	0.0	0.0	0.0
8	0.0	4.9528905812365684E-5	0.0	0.0	0.0
9	0.0	4.9528905812365684E-5	0.0	0.0	0.0
10	9.905781162473137E-5	4.9528905812365684E-5	0.0	9.905781162473137E-5	0.0
11	9.905781162473137E-5	4.9528905812365684E-5	0.0	1.4858671743709704E-4	0.0
12	9.905781162473137E-5	4.9528905812365684E-5	0.0	1.9811562324946274E-4	0.0
13	9.905781162473137E-5	4.9528905812365684E-5	0.0	1.9811562324946274E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	330	0.0	11.827665	4
GTATAAG	265	0.0	11.161363	1
GTAGGAC	790	0.0	10.966151	3
GGCGTGC	80	3.8055624E-4	10.677175	8
AGGACGT	815	0.0	10.618156	5
GTCTTAA	270	0.0	10.601295	1
GGACGTG	775	0.0	10.531229	6
TTAGGAC	660	0.0	10.529769	3
TAGGACG	835	0.0	10.488913	4
GTGCGCC	100	2.4224244E-5	10.440939	11
TGTAGGA	860	0.0	10.412639	2
GTCCTAC	855	0.0	10.37811	1
GTCTTAG	270	0.0	10.247919	1
TGGACCG	130	3.789064E-7	10.241181	5
CCTACAC	265	0.0	10.058936	3
AGGACCT	760	0.0	9.885049	5
GTATTAA	255	0.0	9.728247	1
ATTATAC	245	0.0	9.714352	3
CGTGAAA	480	0.0	9.6895075	9
TGGCGAG	835	0.0	9.662992	18
>>END_MODULE