FastQCFastQC Report
Thu 26 May 2016
SRR1513194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019023
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27700.13719506910025295No Hit
GTACATGGGAAGCAGTGGTATCAAC22490.11139050917201043No Hit
TATCAACGCAGAGTACTTTTTTTTT20550.10178190144441149No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGG752.0360194E-411.4229426
CGAAATC855.3744145E-511.16622613
TTATACC1451.1768861E-911.1611784
TAGGACC4300.011.0695684
GGCGAGG4650.010.61076419
TAATACT1803.45608E-1110.5775874
GGACGTG7950.010.5368866
AGGACGT8100.010.4600595
CGTGAAA5500.010.3505479
GCCTAAG1303.7034624E-710.2582411
GACGTGA5100.010.2354547
AGGACCG856.4695324E-410.0798195
ACGTGAA5850.010.0564078
GTCCTAC9800.010.0115041
CTAGGAC3150.09.9743933
GCGTGCG1151.0421596E-59.9005239
TAGGACG8850.09.8963194
ATAGGAC2600.09.8872043
TAGAAAT3200.09.8173234
TTAGGAC6350.09.7459173
Produced by FastQC (version 0.11.5)