##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513194_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2019023
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.35213021347454	31.0	31.0	34.0	26.0	34.0
2	30.47899701984574	31.0	31.0	34.0	26.0	34.0
3	30.569703762661447	31.0	31.0	34.0	27.0	34.0
4	33.93816266580421	37.0	35.0	37.0	30.0	37.0
5	33.65847243939272	37.0	35.0	37.0	28.0	37.0
6	33.5702862225938	37.0	35.0	37.0	28.0	37.0
7	33.42080402253961	37.0	35.0	37.0	27.0	37.0
8	33.378960021753095	37.0	35.0	37.0	27.0	37.0
9	34.893479172847464	39.0	35.0	39.0	27.0	39.0
10	34.7193578280188	39.0	35.0	39.0	27.0	39.0
11	34.73294657861748	39.0	35.0	39.0	27.0	39.0
12	34.66490723483586	39.0	35.0	39.0	27.0	39.0
13	34.63052030610845	39.0	35.0	39.0	27.0	39.0
14	35.757626832383785	40.0	36.0	41.0	27.0	41.0
15	35.72933146378224	40.0	36.0	41.0	27.0	41.0
16	35.68808775333416	40.0	36.0	41.0	27.0	41.0
17	35.612432844994835	40.0	36.0	41.0	26.0	41.0
18	35.56605893048271	40.0	36.0	41.0	26.0	41.0
19	35.64436264470489	40.0	36.0	41.0	26.0	41.0
20	35.60902476098588	40.0	36.0	41.0	26.0	41.0
21	35.545458372688174	40.0	36.0	41.0	25.0	41.0
22	35.48651303130276	40.0	36.0	41.0	25.0	41.0
23	35.41052528871637	40.0	36.0	41.0	25.0	41.0
24	35.34166921327791	40.0	36.0	41.0	25.0	41.0
25	35.28383232880458	40.0	36.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6092925097139847
1101	2	0.673807130327507
1101	3	0.6572183305881119
1101	4	0.8676670234479573
1101	5	0.8826809901231911
1101	6	0.7561982983548674
1101	7	0.772629028047426
1101	8	0.8232411742130807
1101	9	0.8508997048733349
1101	10	0.8943759514228304
1101	11	0.8600363882492843
1101	12	0.8983567829735506
1101	13	0.9617816032970765
1101	14	1.0608654955762162
1101	15	1.0985640130268166
1101	16	1.030925964618703
1101	17	1.0787587078726233
1101	18	1.0052940192764837
1101	19	1.1477524442621672
1101	20	0.9319088505367787
1101	21	1.0012530882716248
1101	22	0.9977960830661416
1101	23	1.0680045789855939
1101	24	1.1084644154232706
1101	25	1.116218785526172
1102	1	0.7652472598016118
1102	2	0.8192278868447218
1102	3	0.7553855903949049
1102	4	0.8049479260104704
1102	5	0.8718008554269119
1102	6	0.8301937184073083
1102	7	0.8399838286004879
1102	8	0.8722213804210881
1102	9	0.9692733097000499
1102	10	1.1054953973230042
1102	11	1.0312590256886196
1102	12	1.033652673694192
1102	13	1.0441017269412
1102	14	1.2546533375166433
1102	15	1.1306841588209267
1102	16	1.1692941194828919
1102	17	1.2639143343690336
1102	18	1.2409264984651998
1102	19	1.1457184132042357
1102	20	1.2564244337379549
1102	21	1.1902613663536812
1102	22	1.269398124670822
1102	23	1.2399935222599296
1102	24	1.299604724936998
1102	25	1.2631320021039585
1103	1	0.5591970167763769
1103	2	0.5682445957872062
1103	3	0.5767285079905022
1103	4	0.658378618889266
1103	5	0.6565272731613092
1103	6	0.5462834873605331
1103	7	0.6312722453253485
1103	8	0.6898347601289174
1103	9	0.6670794880595423
1103	10	0.7676262405116674
1103	11	0.6870602654554645
1103	12	0.7300151599680973
1103	13	0.7467579485105702
1103	14	0.891061690157116
1103	15	0.9334707386120016
1103	16	1.0074754410813682
1103	17	0.9303633637065474
1103	18	0.861463952448446
1103	19	0.8791806849792678
1103	20	0.9047469823476746
1103	21	0.9722517185749453
1103	22	0.9759949829965819
1103	23	0.8003451942937403
1103	24	0.93608462701701
1103	25	0.8497185931785438
1104	1	0.4515549217330488
1104	2	0.5646880061245838
1104	3	0.5620264726086397
1104	4	0.7596272468351302
1104	5	0.6687496111885025
1104	6	0.5700317373753165
1104	7	0.6616764954302781
1104	8	0.6870299950987615
1104	9	0.7678650213273315
1104	10	0.8163285519475352
1104	11	0.7790591124135631
1104	12	0.9500340518272239
1104	13	0.9418212766139007
1104	14	1.0677693987304764
1104	15	0.9050959549247253
1104	16	0.8752496101259482
1104	17	0.919494347576169
1104	18	0.8474709251594774
1104	19	0.7863071036292482
1104	20	0.9072486513476505
1104	21	0.7963111396690365
1104	22	0.7683216206118502
1104	23	0.7645811772760425
1104	24	0.7357871647420424
1104	25	0.7606664818818345
1105	1	0.5757377773161885
1105	2	0.6166264052912283
1105	3	0.43077478158244986
1105	4	0.5687035063305288
1105	5	0.5118507253689728
1105	6	0.5660899940925432
1105	7	0.6620915513969834
1105	8	0.5584797467475582
1105	9	0.7165852336176286
1105	10	0.7628391089614226
1105	11	0.7260684237346524
1105	12	0.653252620451994
1105	13	0.7829080952705212
1105	14	0.7886291343410647
1105	15	0.8727621502911589
1105	16	0.7505566432201931
1105	17	0.7590919656569142
1105	18	0.8555915729292778
1105	19	0.8303674561452823
1105	20	0.8572571691092108
1105	21	0.9151414052561861
1105	22	0.8492575341191113
1105	23	0.6837927140949631
1105	24	0.8608529089772858
1105	25	0.8000033657935717
1106	1	0.6465554185456028
1106	2	0.6155757792585774
1106	3	0.6278385899857177
1106	4	0.686077975152827
1106	5	0.6996330960457016
1106	6	0.622891357163958
1106	7	0.7064420165438605
1106	8	0.6752983727656883
1106	9	0.7358855061688487
1106	10	0.7372852465323732
1106	11	0.7126361770738399
1106	12	0.6967706113836414
1106	13	0.7523634964104318
1106	14	0.8798379027636898
1106	15	0.85423157239142
1106	16	0.8276845383781364
1106	17	0.750646824341267
1106	18	0.8099224502789397
1106	19	0.7666581432986632
1106	20	0.7988390172726838
1106	21	0.8197377770942893
1106	22	0.8234236003029096
1106	23	0.7180780958496555
1106	24	0.8826428642279751
1106	25	0.9731795024118384
1107	1	0.7219205251363157
1107	2	0.7823208764564491
1107	3	0.6881243330653639
1107	4	0.7921591501409893
1107	5	0.715930555809237
1107	6	0.8086095855768107
1107	7	0.853587761548745
1107	8	0.7367977895003648
1107	9	0.9346804142348049
1107	10	0.934207325729048
1107	11	0.9444608306833047
1107	12	1.020643350107214
1107	13	0.949225258905841
1107	14	1.0138599192065314
1107	15	0.9589957543205188
1107	16	1.0757212109324996
1107	17	1.1293131929060465
1107	18	1.158207174091018
1107	19	1.0585850492429074
1107	20	1.1680058347311046
1107	21	1.0946128410690434
1107	22	1.146187772634029
1107	23	1.17769192405094
1107	24	1.248853881031117
1107	25	1.2483410942420434
1108	1	0.3436634751667178
1108	2	0.44329223018335284
1108	3	0.3959310032774255
1108	4	0.5112503084735991
1108	5	0.4342795899696341
1108	6	0.42777610451876313
1108	7	0.4080897808266144
1108	8	0.46515121759595246
1108	9	0.5147431503318103
1108	10	0.7168447566239848
1108	11	0.5891362577757633
1108	12	0.5150929614161797
1108	13	0.5705876252569837
1108	14	0.7047369167457589
1108	15	0.6731348440730116
1108	16	0.7435909588987286
1108	17	0.6759349736624003
1108	18	0.739059642326005
1108	19	0.731747122721039
1108	20	0.7450068185357139
1108	21	0.6674187229491935
1108	22	0.6534699710914751
1108	23	0.6419734876421614
1108	24	0.6063790621444198
1108	25	0.5397754970463211
1109	1	0.33091275787054286
1109	2	0.3334803249169056
1109	3	0.3360489661226609
1109	4	0.428626703122724
1109	5	0.3963217565572563
1109	6	0.3897922028984979
1109	7	0.3778977506345811
1109	8	0.37297992087919596
1109	9	0.2372647364897631
1109	10	0.42099582441142047
1109	11	0.4501960733810364
1109	12	0.46809198259702356
1109	13	0.4832599697475075
1109	14	0.505014921569213
1109	15	0.47937826395279615
1109	16	0.5073398362839683
1109	17	0.5123880828427829
1109	18	0.5400482160418534
1109	19	0.49411774872802994
1109	20	0.5715514859894668
1109	21	0.49161809260311173
1109	22	0.4922111961963367
1109	23	0.528257237562272
1109	24	0.511829672140486
1109	25	0.47522725067989313
1110	1	0.10116781145893228
1110	2	0.12607970084961195
1110	3	0.1355978740049011
1110	4	0.2149616644576824
1110	5	0.24046773403656374
1110	6	0.1627470258533208
1110	7	0.22282042055725526
1110	8	0.15924365714293742
1110	9	0.26969927725763654
1110	10	0.3176482460638397
1110	11	0.2760202492052102
1110	12	0.2243292638342993
1110	13	0.22961731610485714
1110	14	0.3398812897689183
1110	15	0.3346285692031046
1110	16	0.40589837650917815
1110	17	0.37079264124734124
1110	18	0.31032022798053305
1110	19	0.36274140901836205
1110	20	0.20232750009881784
1110	21	0.3420386596903455
1110	22	0.3253376376561121
1110	23	0.3202461128844831
1110	24	0.3533640636748743
1110	25	0.42044838756769565
1111	1	0.38659662618973556
1111	2	0.3190281911483446
1111	3	0.31207015255213477
1111	4	0.5297727560937062
1111	5	0.4065411742155831
1111	6	0.3801010486048071
1111	7	0.3948206840437507
1111	8	0.36532675849358753
1111	9	0.3979495168776026
1111	10	0.6005728889250719
1111	11	0.6655947757887049
1111	12	0.5756200612819171
1111	13	0.5313362791136385
1111	14	0.5440273834861031
1111	15	0.5331335697186219
1111	16	0.5500175678729278
1111	17	0.5842934603466787
1111	18	0.62690679857009
1111	19	0.6433912851033341
1111	20	0.6291580630052636
1111	21	0.6626202011245539
1111	22	0.7747418335831497
1111	23	0.6924656620552625
1111	24	0.6680104762489094
1111	25	0.7647692033829969
1112	1	-0.5036198076445899
1112	2	-0.4443884958203803
1112	3	-0.26881849429155125
1112	4	-1.1189454201229339
1112	5	-0.5979378929321868
1112	6	-0.42643240714544817
1112	7	-0.37416701854587586
1112	8	-0.33119480033197135
1112	9	-0.22200065035089978
1112	10	-0.14216128613079348
1112	11	-0.18678130036337848
1112	12	-0.15237584856811281
1112	13	-0.18836413602873847
1112	14	-0.29433345852401516
1112	15	-0.09416171077576507
1112	16	-0.1968784230056002
1112	17	-0.23748933327868826
1112	18	-0.2509850683353321
1112	19	-0.331078414502997
1112	20	-0.20622251848818252
1112	21	-0.2915402176550401
1112	22	-0.23510972201206215
1112	23	-0.278087905232951
1112	24	-0.36466141029355015
1112	25	-0.34219223773801133
1113	1	-0.7398248494779409
1113	2	-0.5960809320502101
1113	3	-0.5782216772274822
1113	4	-1.3418774324441713
1113	5	-0.7581182943263087
1113	6	-0.7041967927641366
1113	7	-0.5588145375421405
1113	8	-0.5439856101030713
1113	9	-0.4930855733584565
1113	10	-0.5010797754884493
1113	11	-0.5239815388718938
1113	12	-0.5671931646128101
1113	13	-0.5915314638305347
1113	14	-0.6375625149412727
1113	15	-0.6326818052202441
1113	16	-0.5690583122899113
1113	17	-0.5227655129802713
1113	18	-0.5728586353587204
1113	19	-0.5527784048626856
1113	20	-0.5363525811196155
1113	21	-0.5805997871461486
1113	22	-0.6139763168949415
1113	23	-0.5679643000818615
1113	24	-0.7181566362977065
1113	25	-0.671088544171468
1114	1	-0.6395497861214103
1114	2	-0.6863609449243668
1114	3	-0.5245234679791864
1114	4	-1.3637851334067577
1114	5	-0.7998105425162194
1114	6	-0.8026232248110503
1114	7	-0.6223536319697374
1114	8	-0.6364469267829449
1114	9	-0.6801381220895664
1114	10	-0.5493051164777611
1114	11	-0.5108396229487795
1114	12	-0.600373865280595
1114	13	-0.6679825785538611
1114	14	-0.725700441499086
1114	15	-0.6119993127188934
1114	16	-0.5605873345843762
1114	17	-0.6901903844415642
1114	18	-0.7012431723083594
1114	19	-0.8482294465603388
1114	20	-0.7591782417990842
1114	21	-0.8077785891465084
1114	22	-0.657953998086505
1114	23	-0.7306668448912177
1114	24	-0.6577278944759044
1114	25	-0.7407104064342391
1115	1	-0.6096497379036769
1115	2	-0.5304852245535301
1115	3	-0.4340657364024665
1115	4	-0.32801999798651593
1115	5	-0.46615751227380287
1115	6	-0.5114295937630402
1115	7	-0.5768508304983655
1115	8	-0.5546542798820369
1115	9	-0.7594950364915078
1115	10	-0.6622860041472833
1115	11	-0.6403568771067683
1115	12	-0.7834913417876095
1115	13	-0.784865733720217
1115	14	-0.7874460919015718
1115	15	-0.9066309313397838
1115	16	-0.8561607781938392
1115	17	-0.835806466003234
1115	18	-0.9674766514178685
1115	19	-0.8877481956786326
1115	20	-0.7406531569969346
1115	21	-0.7390306289250859
1115	22	-0.8060704533882443
1115	23	-0.8326814618129106
1115	24	-0.8361280342862827
1115	25	-0.8969009106990811
1116	1	-1.3762082239305116
1116	2	-1.4549120160348537
1116	3	-1.4330216443556303
1116	4	-1.1737359454008
1116	5	-1.6917359809520356
1116	6	-1.600273490092242
1116	7	-1.7392335438276056
1116	8	-1.5645832776529218
1116	9	-1.6513239244937807
1116	10	-1.9039155023280614
1116	11	-1.7736344328992004
1116	12	-1.8626112873201421
1116	13	-1.8242353482596911
1116	14	-2.3091211251993684
1116	15	-2.1920624728444267
1116	16	-2.2463332121788397
1116	17	-2.3335001341606088
1116	18	-2.431345310196285
1116	19	-2.354822971327863
1116	20	-2.272279221186757
1116	21	-2.297400932247726
1116	22	-2.350025736077157
1116	23	-2.4470649214893214
1116	24	-2.3832328623187635
1116	25	-2.4729505110262053
1201	1	0.675756611335089
1201	2	0.6418039935854019
1201	3	0.697476236170619
1201	4	0.8057459440350669
1201	5	0.6762837485882116
1201	6	0.6447567785527255
1201	7	0.6739553328990624
1201	8	0.7264327898493121
1201	9	0.8244082443229246
1201	10	0.9398501501622931
1201	11	0.8908757002675571
1201	12	0.8437272668529943
1201	13	0.8136240501115921
1201	14	0.9063486453167329
1201	15	1.035673501282517
1201	16	0.9151624734169559
1201	17	1.0158176035136819
1201	18	1.003567838182164
1201	19	0.9139305030235434
1201	20	0.9425496449186639
1201	21	1.07083427871585
1201	22	0.9686510398775638
1201	23	0.9949568215032372
1201	24	0.9760507001546159
1201	25	1.0884492776950196
1202	1	0.5368515576924864
1202	2	0.5761747543124898
1202	3	0.6131387695362598
1202	4	0.6951340005186424
1202	5	0.7289422198837414
1202	6	0.6840731907278723
1202	7	0.6787575049331664
1202	8	0.6913597050228333
1202	9	0.7796932284009088
1202	10	0.7549586087698188
1202	11	0.7557287769660803
1202	12	0.7527500613816969
1202	13	0.7290503766760779
1202	14	0.8286127581007108
1202	15	0.8259655543927948
1202	16	0.9063279956190584
1202	17	0.9645055873035631
1202	18	0.9763874567024544
1202	19	0.9827445273730717
1202	20	1.0436287932767598
1202	21	0.8765032419435244
1202	22	0.9335676520080582
1202	23	0.9724612718454324
1202	24	0.944069863814633
1202	25	0.9464590350583606
1203	1	0.5340977110554981
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2211	4	-1.383216097526045
2211	5	-0.8135492182390891
2211	6	-0.719344218604661
2211	7	-0.7622674620252994
2211	8	-0.6558847822925387
2211	9	-0.6860998638471187
2211	10	-0.5328326262187417
2211	11	-0.6410965477623307
2211	12	-0.6188496827028516
2211	13	-0.6459611408168868
2211	14	-0.7697825978978159
2211	15	-0.782205627162007
2211	16	-0.7913648350219802
2211	17	-0.7737036979185064
2211	18	-0.7240798615190585
2211	19	-0.6241462426702853
2211	20	-0.6914786016674839
2211	21	-0.6334160772071797
2211	22	-0.7231686028721569
2211	23	-0.7437019096634003
2211	24	-0.5592519943569627
2211	25	-0.6419133675268256
2212	1	-0.0868800911212162
2212	2	-0.2678719256179072
2212	3	-0.308487469285712
2212	4	0.0420708581200131
2212	5	-0.13835118479143205
2212	6	-0.18219696445851952
2212	7	-0.18520006586922477
2212	8	-0.3037899486699587
2212	9	-0.42412367830464603
2212	10	-0.35303244825446
2212	11	-0.4056176557296993
2212	12	-0.40494967450457864
2212	13	-0.40226783880482486
2212	14	-0.44154132267095036
2212	15	-0.4301004650082234
2212	16	-0.4679847936728123
2212	17	-0.47154260953477234
2212	18	-0.5037032187177601
2212	19	-0.5119390161843214
2212	20	-0.5444145189411103
2212	21	-0.47690203395516306
2212	22	-0.488865780112242
2212	23	-0.5212531975067449
2212	24	-0.595853819057993
2212	25	-0.6103426441543078
2213	1	-0.4116834131558065
2213	2	-0.3763635191678887
2213	3	-0.5466194843127532
2213	4	-0.30382925149629614
2213	5	-0.6471860235644584
2213	6	-0.6786263980725877
2213	7	-0.7709042974677374
2213	8	-0.7239122094020374
2213	9	-0.9202156325817228
2213	10	-0.724321522609614
2213	11	-0.7920069961722049
2213	12	-0.9522532892085493
2213	13	-0.9433801261198482
2213	14	-0.9886946190676156
2213	15	-0.9691235857507579
2213	16	-0.9383606198363239
2213	17	-0.9142550776001954
2213	18	-0.8944538164218514
2213	19	-0.9343609996472821
2213	20	-0.9468024431493731
2213	21	-0.9510938450890478
2213	22	-0.9825600100169112
2213	23	-0.864252679590173
2213	24	-0.9793247553540709
2213	25	-1.1463686094313346
2214	1	-0.5738561663203825
2214	2	-0.6219157567998757
2214	3	-0.6116822785321396
2214	4	-0.42628606244883827
2214	5	-0.5788015767077823
2214	6	-0.6216566755485147
2214	7	-0.7775484535996853
2214	8	-0.7782123196542443
2214	9	-1.0087377935401136
2214	10	-1.0244764920988132
2214	11	-1.0843243402568419
2214	12	-1.0764827196843285
2214	13	-1.0885455639909054
2214	14	-1.1613574982214772
2214	15	-1.012398569806635
2214	16	-1.0531435481583955
2214	17	-1.1846340097666328
2214	18	-1.2535820884647748
2214	19	-1.2538126579966544
2214	20	-1.2362878502836736
2214	21	-1.2415233257465133
2214	22	-1.3364166534072695
2214	23	-1.2401069167558916
2214	24	-1.285118545721346
2214	25	-1.2690587443829813
2215	1	-0.9397639649338387
2215	2	-0.8571688604131324
2215	3	-0.957115906829447
2215	4	-0.7803764686464021
2215	5	-0.9701645939716457
2215	6	-1.1355229075229971
2215	7	-1.1903413388811046
2215	8	-1.082084518227795
2215	9	-1.5080313385935469
2215	10	-1.442185911110741
2215	11	-1.4099780314739192
2215	12	-1.3634116201831787
2215	13	-1.4987550601398638
2215	14	-1.6533214229373385
2215	15	-1.517273429507405
2215	16	-1.5725784723577405
2215	17	-1.7181249029893522
2215	18	-1.6139514485961115
2215	19	-1.6001827498277876
2215	20	-1.5650961453390124
2215	21	-1.644019319630047
2215	22	-1.685923317044555
2215	23	-1.679927013175167
2215	24	-1.6708711967306016
2215	25	-1.8682082736066974
2216	1	-1.9066511185822215
2216	2	-1.6141466365989459
2216	3	-1.7030852535671173
2216	4	-1.619811998093752
2216	5	-2.0356639532062317
2216	6	-2.2049484473977117
2216	7	-2.536541719360443
2216	8	-2.361949758494923
2216	9	-2.5491278832361886
2216	10	-2.614656280685132
2216	11	-2.66770403063018
2216	12	-2.680711888429073
2216	13	-2.647234535746996
2216	14	-2.835633113523265
2216	15	-2.941708879356568
2216	16	-2.8979082078529643
2216	17	-2.9379522147197363
2216	18	-3.0709554921840763
2216	19	-3.015859414026906
2216	20	-3.0285366974320453
2216	21	-3.264854344424883
2216	22	-3.156349605142239
2216	23	-3.0933341340290994
2216	24	-2.995812891057639
2216	25	-3.158461077452884
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65835.0
3	0.0
4	1059.0
5	8762.0
6	9127.0
7	7167.0
8	5616.0
9	4784.0
10	4486.0
11	3855.0
12	3571.0
13	3477.0
14	3252.0
15	3196.0
16	3434.0
17	3388.0
18	3927.0
19	4403.0
20	5793.0
21	7278.0
22	9167.0
23	10923.0
24	12781.0
25	13903.0
26	15857.0
27	17259.0
28	19274.0
29	23313.0
30	29307.0
31	37287.0
32	47420.0
33	62098.0
34	82206.0
35	110119.0
36	159385.0
37	262010.0
38	954304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.17409750651284	19.474754993177026	13.55876442128768	27.792383079022454
2	13.981330224015517	20.68069492064404	39.03187810258966	26.306096752750786
3	16.823895009836885	24.699692708818326	29.925376387119528	28.55103589422526
4	11.31170576281193	17.346781595019706	37.15037714270997	34.1911354994584
5	12.733327947110599	38.13143341761285	35.971376356696155	13.163862278580396
6	32.09979850481129	37.5656598532638	17.630207880424656	12.704333761500259
7	27.841554000660913	31.56365668547172	22.37071504620673	18.224074267660644
8	25.34186522738013	36.60466876496574	20.229911362220115	17.82355464543402
9	26.185898102662097	14.781711536880355	19.583651570581306	39.448738789876245
10	14.084655633901876	29.18538095926806	34.93255920386576	21.797404202964305
11	34.65834713126101	22.30895834272319	24.133949935191428	18.898744590824375
12	22.636542525766178	24.842015172685006	31.601026833275302	20.920415468273518
13	28.635830300100594	21.489997885115724	25.846709027088842	24.02746278769484
14	21.04617972335851	21.285357178765953	27.668210497953694	30.000252599921843
15	23.348371960101495	29.87410247431555	23.22618414946239	23.551341416120568
16	22.748081621655622	27.980810520732057	26.51198128996054	22.75912656765178
17	22.08966433217922	28.19556191428301	27.201741031907194	22.513032721630577
18	22.115054963130014	27.55231257344767	28.28911775605367	22.043514707368647
19	23.34822337338406	27.153826380382988	27.326434617139086	22.171515629093875
20	23.55926604105055	27.048329811002645	27.41855838195008	21.97384576599672
21	23.30303474563847	26.607020449565532	27.0420838665964	23.047860938199598
22	23.19126627086467	27.34778157554421	26.828570055913183	22.632382097677937
23	22.621466990073422	27.081765872200176	27.10717545939097	23.189591678335432
24	22.917321892816474	27.025843687763835	27.42955379904043	22.62728062037926
25	22.897186309602525	26.94801094193671	27.15836041328941	22.996442335171352
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	122.0
1	122.0
2	222.5
3	323.0
4	323.0
5	323.0
6	832.0
7	1341.0
8	1341.0
9	1341.0
10	2136.0
11	2931.0
12	2931.0
13	2931.0
14	5464.0
15	7997.0
16	7997.0
17	7997.0
18	15027.0
19	22057.0
20	22057.0
21	22057.0
22	34796.0
23	47535.0
24	47535.0
25	47535.0
26	67546.5
27	87558.0
28	87558.0
29	87558.0
30	112228.0
31	136898.0
32	136898.0
33	136898.0
34	159782.0
35	182666.0
36	182666.0
37	182666.0
38	201743.5
39	220821.0
40	220821.0
41	220821.0
42	233711.5
43	246602.0
44	246602.0
45	246602.0
46	257492.5
47	268383.0
48	268383.0
49	268383.0
50	262044.5
51	255706.0
52	255706.0
53	255706.0
54	230667.0
55	205628.0
56	205628.0
57	205628.0
58	177118.0
59	148608.0
60	148608.0
61	148608.0
62	121520.0
63	94432.0
64	94432.0
65	94432.0
66	73000.5
67	51569.0
68	51569.0
69	51569.0
70	37618.0
71	23667.0
72	23667.0
73	23667.0
74	16398.5
75	9130.0
76	9130.0
77	9130.0
78	6279.5
79	3429.0
80	3429.0
81	3429.0
82	2302.5
83	1176.0
84	1176.0
85	1176.0
86	763.5
87	351.0
88	351.0
89	351.0
90	218.5
91	86.0
92	86.0
93	86.0
94	46.0
95	6.0
96	6.0
97	6.0
98	3.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18687256163005572
2	0.007181691342793024
3	0.004507130428925277
4	0.0012877515511215078
5	0.0757792258929195
6	0.32530585337561785
7	0.029420170052545217
8	0.012134581924029593
9	0.0044080726173005465
10	0.0033184366894285005
11	0.0
12	0.0
13	0.0
14	9.905781162473138E-4
15	0.0
16	0.0
17	0.022288007615564556
18	0.02867723646535973
19	0.0
20	0.0
21	3.467023406865598E-4
22	0.0
23	0.004952890581236568
24	0.0
25	9.905781162473137E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2019023.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.83431297090246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.52585503157533	60.107139535610834
2	11.834576328586657	17.239264723886286
3	3.0269797423865423	6.614039697406893
4	1.111405731840575	3.237938916421253
5	0.5164274568871001	1.8806819510841146
6	0.2831986587860066	1.237594784817589
7	0.16080702369400515	0.8198588364153948
8	0.10636540697700118	0.6197641072832254
9	0.0850115196066942	0.5572580062649377
>10	0.3200649261217113	3.9880514616560307
>50	0.0176557191619715	0.9034579490585929
>100	0.009813981607122749	1.3976876194397883
>500	0.0012256485128953332	0.5912105202463318
>1k	6.128242564476145E-4	0.8060518904087731
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2770	0.13719506910025295	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2249	0.11139050917201043	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2055	0.10178190144441149	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.905781162473137E-5	0.0
11	0.0	0.0	0.0	1.4858671743709704E-4	0.0
12	0.0	0.0	0.0	1.4858671743709704E-4	9.905781162473137E-5
13	0.0	0.0	0.0	1.4858671743709704E-4	9.905781162473137E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGG	75	2.0360194E-4	11.422942	6
CGAAATC	85	5.3744145E-5	11.166226	13
TTATACC	145	1.1768861E-9	11.161178	4
TAGGACC	430	0.0	11.069568	4
GGCGAGG	465	0.0	10.610764	19
TAATACT	180	3.45608E-11	10.577587	4
GGACGTG	795	0.0	10.536886	6
AGGACGT	810	0.0	10.460059	5
CGTGAAA	550	0.0	10.350547	9
GCCTAAG	130	3.7034624E-7	10.258241	1
GACGTGA	510	0.0	10.235454	7
AGGACCG	85	6.4695324E-4	10.079819	5
ACGTGAA	585	0.0	10.056407	8
GTCCTAC	980	0.0	10.011504	1
CTAGGAC	315	0.0	9.974393	3
GCGTGCG	115	1.0421596E-5	9.900523	9
TAGGACG	885	0.0	9.896319	4
ATAGGAC	260	0.0	9.887204	3
TAGAAAT	320	0.0	9.817323	4
TTAGGAC	635	0.0	9.745917	3
>>END_MODULE